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CN-122012723-A - Structural variation molecular marker located on chromosome 6 of pig and related to lactation power of sow and application thereof

CN122012723ACN 122012723 ACN122012723 ACN 122012723ACN-122012723-A

Abstract

The invention discloses a structural variation molecular marker related to lactation capacity of a sow, which is positioned on a chromosome 6 of the sow, wherein the structural variation molecular marker is a DNA fragment which is deleted after 147139167 bp th site on chromosome 6 of an international pig reference genome 11.1 version 6, has a nucleotide sequence shown as SEQ ID NO. 1 and has the size of 283 bp, and the genotype of A/A, A/DEL and DEL/DEL. The structural variation molecular marker provided by the invention is obviously related to the lactation performance of the sow, and by breeding individuals with the genotype of the structural variation molecular marker A/A, mutant individuals with the deletion type structural variation, namely individuals with the genotypes of A/DEL and DEL/DEL, are eliminated, so that the frequency of wild individuals in the population can be increased generation by generation, thereby improving the lactation performance of offspring pigs, increasing the reproductive performance of the sow and improving the economic effect of cultivation.

Inventors

  • LIU LANGQING
  • ZHENG ENQIN
  • CAI GENGYUAN
  • WU JIAJIN
  • WU ZHENFANG
  • LUO JUN
  • YANG JIE
  • DENG SHAOXIONG
  • Liu Yidie
  • WU DAIQI
  • QIU YIBIN
  • ZHANG ZEBIN

Assignees

  • 华南农业大学

Dates

Publication Date
20260512
Application Date
20260107

Claims (10)

  1. 1. The structural variation molecular marker is located on a chromosome 6 of a pig and is related to the lactation capacity of a sow, and is characterized by being a DNA fragment deleted after 147139167 bp th site on chromosome 6 of an international pig reference genome 11.1 version 6, wherein the nucleotide sequence of the DNA fragment is shown as SEQ ID NO. 1, and the genotypes of the structural variation molecular marker are A/A, A/DEL and DEL/DEL.
  2. 2. The use of a structurally mutated molecular marker product according to claim 1, characterized in that it comprises at least one of the following (1) - (6): (1) Identifying the total weight gain character of 7-day-old litter of pigs; (2) Preparing a product for identifying the total weight gain character of the 7-day-old litter of the pig; (3) Identifying the lactation performance of the pigs; (4) Preparing a product for identifying the lactation performance of pigs; (5) Pig genetic improvement, based on breeding pigs with the structural variation molecular marker genotype A/A to improve total weight gain and/or lactation capacity of 7-day litter of the pigs; (6) A product for assisting in genetic improvement of pigs is prepared, which realizes assisting in genetic improvement of pigs based on identification of genotypes of the structural variation molecular markers.
  3. 3. The use according to claim 2, wherein the product for detecting the structural variant molecular marker according to claim 1 comprises at least one of a reagent, a kit, a chip and a device for detecting the structural variant molecular marker.
  4. 4. The method of claim 3, wherein the reagent for detecting the structural variant molecular marker comprises at least one of a primer and a probe for detecting the structural variant molecular marker.
  5. 5. The use according to any one of claims 2 to 4, wherein the pig is a white pig.
  6. 6. The primer pair for detecting the structural mutation molecular marker according to claim 1, wherein the primer pair comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is shown as SEQ ID NO.3, and the nucleotide sequence of the downstream primer is shown as SEQ ID NO. 4.
  7. 7. Kit for detecting a structurally mutated molecular marker according to claim 1, characterized in that its composition comprises a primer pair according to claim 6.
  8. 8. A method for genetic modification of pigs comprising the steps of: (1) Determining the genotype of a pig with the structural variant molecular marker of claim 1; (2) And breeding individuals with the structural variation molecular marker genotype of A/A.
  9. 9. The method for genetic modification of swine according to claim 8, wherein in step (1), the method for determining the genotype of swine according to the structural variant molecular marker of claim 1 comprises the steps of: Extracting pig whole genome DNA, carrying out PCR amplification by using a primer pair with nucleotide sequences shown as SEQ ID NO. 3 and SEQ ID NO. 4, sequencing the amplified products, and determining the genotype of the pig structurally mutated molecular marker according to claim 1 based on the sequencing result.
  10. 10. The method of genetic modification of pigs according to claim 8 or 9, wherein the pigs are white pigs.

Description

Structural variation molecular marker located on chromosome 6 of pig and related to lactation power of sow and application thereof Technical Field The invention belongs to the technical field of genetic breeding, and relates to a structural variation molecular marker related to lactation performance of a sow on a chromosome 6 of the sow and application thereof. Background Pigs are the first six animals and are also the core source of animal protein supply in daily life. With the rapid development of large-scale and intensive pig industry, the industry has the theory that the Sow is obtained from the world, and the annual productivity (PIGS WEANED PER Sow per Year, PSY) of the Sow has become the primary index for measuring the economic benefit of a pig farm, and the lactation power is a key factor for determining the PSY. The lactation power of the sow directly influences the weaning weight, the survival rate and the subsequent growth potential of the piglet, thereby obviously influencing the reproductive performance of the whole pig group and the economic benefit of a farm. At present, the milk yield of sows can not meet the maximum growth requirement of piglets, and the assessment of the lactation power in traditional breeding mainly refers to the number of weaned piglets and the weight of weaned litter, so that the method has the advantages of high measurement cost, long period, easiness in interference by various factors such as environment, feeding management and the like, and slow genetic progress. Disclosure of Invention The invention aims to provide a structural variation molecular marker which is positioned on a chromosome 6 of a pig and affects the lactation capacity of the sow and application thereof. According to one aspect of the present invention, there is provided a structural variation molecular marker related to sow lactation on chromosome 6 of swine, which is a 283 bp sized DNA fragment (designated as g.147139168_147139450 DEL) deleted after 147139167 bp th site on chromosome 6 of International swine reference genome 11.1 version 6, the genotype of which is designated as A/A, A/DEL or DEL/DEL. The deletion type structural variation provided by the invention causes a significant difference in the lactation capacity of sows, and the deletion type structural variation generated after 147139167 bp th site on chromosome 6 causes a significant reduction in the lactation capacity of sows, and the total weight gain of 7-day litter of sows carrying g.147139168_147139450del is significantly lower than that of sows not carrying g.147139168_147139450 del. Thus, the total weight gain and lactation traits of the 7-day litter of the pig can be identified by detecting the above structural variation molecular markers of the pig, i.e., detecting whether the pig carries g.147139168_147139450del, or by identifying the genotype of the pig with the above structural variation molecular markers. According to a second aspect of the present invention, there is provided an application of a product for detecting a structural variant molecular marker of the present invention, the application comprising at least one of the following (1) to (6): (1) Identifying the total weight gain character of 7-day-old litter of pigs; (2) Preparing a product for identifying the total weight gain character of the 7-day-old litter of the pig; (3) Identifying the lactation performance of the pigs; (4) Preparing a product for identifying the lactation performance of pigs; (5) Pig genetic improvement, based on breeding pigs with structural variation molecular marker genotype A/A to improve total weight gain and lactation capacity of 7-day litter of pigs; (6) A product for assisting in genetic improvement of pigs is prepared, and the product realizes assisting in genetic improvement of pigs based on identification of genotypes of the structural variation molecular markers. In some embodiments, products for detecting the structural variant molecular markers of the present invention may include at least one of reagents, kits, chips, and devices for detecting the structural variant molecular markers of the present invention. In some embodiments, the reagents for detecting the structural variant molecular markers of the present invention may include at least one of primers, probes for detecting the structural variant molecular markers of the present invention. In some embodiments, the pig is a white pig. According to a third aspect of the present invention, there is provided a primer set for detecting the structural variant molecular marker of the present invention, the primer set comprising an upstream primer and a downstream primer, wherein the nucleotide sequence of the upstream primer is shown as SEQ ID NO.3, and the nucleotide sequence of the downstream primer is shown as SEQ ID NO. 4. According to a fourth aspect of the present invention, there is provided a kit for detecting the structural variant molecular marker of the present inventio