CN-122012727-A - Molecular marker related to chicken breast muscle color traits and application thereof

Abstract

The invention discloses a molecular marker related to chicken breast muscle color traits and application thereof, and belongs to the technical field of genetic engineering. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, and the mutation site of the molecular marker is positioned at the 392bp position of the CDS sequence of the INHA gene, wherein the mutation site has C/T mutation, and the mutation site has CC, CT and TT genotypes. Experiments show that the molecular marker is obviously related to chicken breast muscle redness, and the chicken breast muscle redness of a chicken individual with the mutation site genotype TT is extremely higher than that of a chicken individual with CC and CT genotypes. The invention can provide theoretical basis for breeding and marking auxiliary selection of the meat quality character of the local chicken species.

Inventors

• WU YANPING
• ZHOU MIN
• WEN ZHENGYA
• ZHU XUENONG
• TAN YUWEN
• NIE RUI

Assignees

• 江西省农业科学院畜牧兽医研究所
• 南昌师范学院

Dates

Publication Date

20260512

Application Date

20260212

Claims (9)

1. 1. A molecular marker related to chicken breast muscle color traits is characterized in that the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, the 130 th base of the molecular marker has C/T mutation, and mutation sites have CC, CT and TT genotypes.
2. 2. Use of a molecular marker according to claim 1 for identifying the muscle color trait of chicken breast.
3. 3. The use of a molecular marker according to claim 1 in chicken breeding, wherein the character index of the chicken breeding is chicken breast muscle color.
4. 4. The use according to claim 2 or 3, wherein the chicken individuals of the TT genotype have a higher pectoral muscle tone than the chicken individuals of the CC and CT genotypes.
5. 5. Use according to claim 2 or 3, wherein the chicken pectoral muscle is 16w Ningdu yellow cock's pectoral muscle redness.
6. 6. A method for detecting chicken breast muscle tone, comprising the steps of: Extracting genomic DNA of the chicken to be detected; Amplifying the molecular marker of claim 1 by using a primer pair, sequencing, analyzing the genotype of the mutation site of the molecular marker according to the sequencing result, and judging the pectoral muscle flesh color of the chicken to be detected according to the genotype result.
7. 7. The method of claim 6, wherein the primer pair has a nucleotide sequence set forth in SEQ ID NO. 2-3.
8. 8. The method of claim 6, wherein the mutation site of the molecular marker is a chicken individual with a TT genotype that has a higher muscle tone than chicken individuals with CC and CT genotypes.
9. 9. The method of claim 6, wherein the chicken pectoral muscle meat color is 16w Ningdu yellow cock's pectoral muscle redness.

Description

Molecular marker related to chicken breast muscle color traits and application thereof Technical Field The invention relates to the technical field of genetic engineering, in particular to a molecular marker related to chicken breast muscle color traits and application thereof. Background Inhibin (inhibin, INH) is a heterodimeric glycoprotein hormone secreted by female ovarian granulosa cells and male testis support cells, belongs to a transforming growth factor beta superfamily member, is formed by joining two different subunits, alpha and beta, via disulfide bonds, and plays a key role in follicular development, oocyte maturation and embryo development. INH exists in two forms, which are classified into INHA (αβA) and INHB (αβB). The functional activities of INHA and INHB are substantially identical, while at the amino acid level, the remaining amino acids are approximately identical except for the significant differences in histidine, isoleucine and lysine levels. Inhibin INHA is mainly derived from dominant follicles and luteal cells, produced and secreted by the granular layer of large preovulatory follicles, and mRNA encoding alpha-and beta-subunits is also found in chicken musculature such as skeletal and cardiac muscles, while mRNA of beta-subunits is also detected in small intestinal muscles. The chicken INHA gene is located on chromosome 7 and contains 2 exons and 1 intron, the full length of which is 1679 bp, the full length of which is 987, 987 bp, and the total code of which is 329 amino acids. Researchers at home and abroad have found important regulating effects of inhibin on reproductive function on animals such as pigs, cattle, sheep, mice and the like, and are also mostly related to growth and reproduction traits of hens on chickens. Active immunization against INHA resulted in increased ovulation in sheep, pigs, chickens, mice and cattle. INHA is believed to be a functional center of inhibin and has the potential to increase the ovulation rate of poultry. Numerous studies have reported that the INHA gene is not only closely related to human reproductive performance but also to animals, and that the INHA gene has been shown to act as a major or candidate gene affecting reproductive performance. Through single nucleotide polymorphism analysis related to chicken reproduction traits, INHA is found to be a candidate gene for improving chicken reproduction traits. Gui Taotao and the like, through SNPs screening, INHA is possibly one of main genes affecting follicular development, plays a key role in the chicken follicular development process, and can be used as a proper molecular marker for breeding egg yield. Jin Hengdeng through researching the relationship between the INHA gene polymorphic locus of Ningdu yellow hen and the growth and reproduction traits thereof, and then carrying out correlation analysis, the INHA gene is found to be closely related to the growth and development of the organism. At present, the polymorphism research of INHA genes at home and abroad mainly focuses on the characters in the aspect of growth and propagation, and few researches and reports on the correlation of the characters of the meat quality of poultry are provided. The quality of the poultry meat is determined by the nutritional value, organoleptic properties and safety, the content of high-value proteins, cholesterol, vitamins, micronutrients, unsaturated fatty acids and other bioactive substances in the poultry meat determines the nutritional quality, and the basic organoleptic characteristics comprise meat color, aroma, flavor and the like. Mass production of broiler chickens has been achieved, and emphasis is now being placed on improving the quality of meat by changing various characteristics of broiler chickens. Appearance, texture, juiciness, water feel, firmness, tenderness, smell and flavor are the most important and easily perceived meat characteristics affecting the consumer's preliminary and final judgment of quality before and after purchasing a meat product. The quantifiable properties of meat, such as water retention, shear, drip loss, cook loss, pH, shelf life, collagen content, protein solubility, tack and fat binding capacity, are essential to the processors involved in the manufacture of value added meat products. Common indexes for evaluating chicken quality include meat color, tenderness, water loss rate, water retention capacity, pH value and the like. Along with the improvement of the living standard of people, the requirements on chicken quality are also increasingly improved, so the research on the quality character of the bred chicken is a trend. At present, research and report on the chicken quality and characteristics of the INHA gene are not yet reported. Disclosure of Invention The invention aims to provide a molecular marker related to chicken breast muscle color traits and application thereof, so as to solve the problems in the prior art, and the C+392T locus on Ningdu Huang