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CN-122012729-A - Molecular biological identification method and application of fast large-size and small-size white-feather broiler chickens

CN122012729ACN 122012729 ACN122012729 ACN 122012729ACN-122012729-A

Abstract

The invention discloses a molecular biological identification method and application of quick-size and small-size white-feather broiler chickens, wherein molecular markers are formed by amplifying genome DNA of chickens to be detected by adopting sequences shown as SEQ ID NO.1 and SEQ ID NO.2, alleles are detected by capillary electrophoresis, the small-size white-feather broiler chickens contain 309 or 311 bp alleles, the quick-size white-feather broiler chickens do not contain 309 or 311 bp alleles, and the quick-size and small-size white-feather broiler chickens are identified by utilizing the difference of the alleles. The method is simple to operate, easy to operate in a laboratory, convenient for popularization and use in a basic layer, has wide market application prospect, and can generate considerable economic benefit and good social value based on the detection kit developed by the method.

Inventors

  • ZHANG JING
  • JIA XIAOXU
  • FAN YANFENG
  • MA LINA
  • GAO YUSHI
  • TANG MENGJUN
  • LIU YINYIN
  • ZHANG XIAOYAN
  • ZHANG WENTAO

Assignees

  • 江苏省家禽科学研究所

Dates

Publication Date
20260512
Application Date
20260212

Claims (10)

  1. 1. A molecular marker for identifying fast-size and small-size white-feather broiler chickens is characterized in that the molecular marker is formed by amplifying genome DNA of chickens to be detected by adopting sequences shown in SEQ ID NO.1 and SEQ ID NO.2, detecting alleles by capillary electrophoresis, wherein the small-size white-feather broiler chickens contain 309 or 311 bp alleles, and the fast-size white-feather broiler chickens do not contain 309 or 311 bp alleles.
  2. 2. A primer pair for identifying molecular markers of fast large-size and small-size white-feather broiler chickens is shown as SEQ ID NO.1 and SEQ ID NO. 2.
  3. 3. A kit for identifying fast large and small white-feather broiler chicks containing the primer pair of claim 2.
  4. 4. Use of the molecular marker of claim 1, the primer pair of claim 2 or the kit of claim 3 for identifying or screening fast large and small white broiler chicks.
  5. 5. A method for identifying fast-size and small-size white-feather broiler chickens is characterized in that the genome DNA of the white-feather broiler chickens to be detected is amplified through primer pairs SEQ ID NO.1 and SEQ ID NO.2, alleles are detected through capillary electrophoresis, the small-size white-feather broiler chickens contain 309 or 311 bp alleles, and the fast-size white-feather broiler chickens do not contain 309 or 311 bp alleles.
  6. 6. A method for screening fast-growing white-feather broiler chickens is characterized in that the genome DNA of the white-feather broiler chickens to be detected is amplified through primer pairs SEQ ID NO.1 and SEQ ID NO.2, alleles are detected through capillary electrophoresis, the fast-growing white-feather broiler chickens without 309 and 311 bp alleles are detected, and the small white-feather broiler chickens with 309 or 311 bp alleles are detected.
  7. 7. The identification or screening method for the fast large-size and small-size white-feather broiler chickens is characterized by comprising the following steps of: 1) Extracting genome DNA of fast large-sized and small-sized white-feather broiler chickens to be detected; 2) Taking the DNA extracted in the step 1) as a template, and carrying out PCR amplification reaction by using the primer pair; 3) After the reaction is finished, the PCR product is subjected to genotyping in an ABI 3730 analyzer, the small white-feather broiler chicks containing 309 or 311 bp alleles and the fast large white-feather broiler chicks without 309 or 311 bp alleles are obtained.
  8. 8. The method according to claim 7, wherein the PCR reaction system comprises 2 XPCR Mix 25. Mu.L, 10. Mu. Mol/L forward and reverse primers 1. Mu.L each, 50-100. Mu.g/ml template DNA 2. Mu.L, and ultra pure water 21. Mu.L.
  9. 9. The method of claim 7, wherein the PCR reaction is performed using a cycle of 95℃5min, (95℃30 s,58℃30 s,72℃60 s) 35, 72℃10 min.
  10. 10. The use according to any one of claims 5 to 9, wherein the chicken genomic DNA to be detected is taken from chicken blood, chicken, feathers.

Description

Molecular biological identification method and application of fast large-size and small-size white-feather broiler chickens Technical Field The invention belongs to the technical field of molecular biology, and particularly relates to a molecular biology identification method and application of fast large-sized and small-sized white feather broiler chickens. Background The Chinese broiler consists of 3 types of fast large white-feather broiler and small white-feather broiler and yellow-feather broiler. The white feather broiler chicken has high growth speed and high feed conversion efficiency, is 38-42 days old in the market, and has a weight of about 2.5-3.0 kg. Yellow feather broilers mainly refer to local chicken varieties in China and cultivated varieties containing blood-related varieties of the local chickens, the commercial ages of 60-150 days, and the weight of 1.0-2.5 kg. The small white-feather broiler chicken is also called 817 broiler chicken, is usually obtained by hybridization of fast large white-feather broiler chicken and high-yield brown-shell layer chicken, has the advantage of low seed production cost, is 35-49 days old in the market, and has a weight of 1.1-2.0 kg. The size of the quick large white feather broiler breeder is larger, the feed consumption is more, the egg laying rate is relatively less, the egg laying rate is about 180 eggs in one production period, and the cost of each chick is about 2.5 yuan. The small white feather broiler breeder is derived from high-yield laying hens, has relatively small body size and more eggs, generally lays about 300 eggs in one production period, and has the cost of about 0.8 yuan per chick. Fast large white feather broiler and small size the body forms and the appearance of the white feather broiler chickens are similar, the identification is impossible by the appearance. Some manufacturers can blend small white-feather broiler chickens into fast large white-feather broiler chickens to achieve profit, so that certain market confusion is caused. Therefore, a rapid, accurate and easy-to-operate technology is needed to identify a large and small white-feather broiler chicken, and ensure the orderly, healthy and stable development of the broiler chicken industry in China. Disclosure of Invention One of the purposes of the invention is to provide a molecular biology identification method for fast large-sized and small-sized white feather broiler chickens aiming at the defects of the prior art. The second object of the present invention is to provide a primer for detecting the above molecular marker. It is a further object of the present invention to provide the use of the molecular marker. The fourth object of the present invention is to provide a detection method using the above molecular marker. The aim of the invention is achieved by the following technical scheme: a molecular marker for identifying quick-large and small white-feather broiler chickens is characterized in that genome DNA of chickens to be detected is amplified by adopting sequences shown in SEQ ID NO.1 and SEQ ID NO.2, alleles are detected by capillary electrophoresis, 309 or 311 bp alleles are small white-feather broiler chickens, and 309 or 311 bp alleles are absent. The invention also provides a primer pair for identifying molecular markers of fast large-sized and small-sized white-feather broiler chickens, wherein: Forward primer (SEQ ID NO. 1): 5'-AACTTGGTAAGGGAATTCCCT-3' Reverse primer (SEQ ID NO. 2): 5'-GAATTAGTGCCCAGCTGTGCT-3'. The invention also provides a kit containing the primer pair for identifying genotypes of the fast large-size and small-size white-feather broiler chickens. The invention also provides application of the molecular marker or the primer or the kit pair in identifying fast large-sized and small-sized white-feather broiler chickens. The invention also provides application of the molecular marker or the primer or the kit to screening of fast large-size or small-size white feather broilers. The invention also provides a method for identifying the fast-size and small-size white-feather broiler chickens, wherein the primer pairs SEQ ID NO.1 and SEQ ID NO.2 are used for amplifying genome DNA of the white-feather broiler chickens to be detected, capillary electrophoresis is used for detecting alleles, 309 or 311 bp alleles are small-size white-feather broiler chickens, and 309 or 311 bp alleles are absent. The invention also provides a method for screening the fast large-size and small-size white-feather broiler chickens, which comprises the steps of amplifying genome DNA of the fast large-size white-feather broiler chickens to be detected through primer pairs SEQ ID NO.1 and SEQ ID NO.2, detecting alleles by capillary electrophoresis, wherein the small-size white-feather broiler chickens contain 309 or 311 bp alleles, and the fast large-size white-feather broiler chickens do not contain 309 or 311 bp alleles. In a specific embodiment, the invention also prov