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CN-122012734-A - Molecular marker related to pheasant weight traits and application thereof

CN122012734ACN 122012734 ACN122012734 ACN 122012734ACN-122012734-A

Abstract

The invention relates to the technical field of animal breeding, in particular to a molecular marker related to pheasant weight characteristics and application thereof. Based on genomic version ASM414374v1, 9 SNP molecular markers were included. According to the invention, 9 SNP molecular markers related to the weight of the pheasant are obtained through research and screening, the SNP molecular markers are closely connected with the weight characters of the pheasant, and the prediction and identification of the weight of the pheasant can be realized by detecting the polymorphism of the SNP molecular markers. The molecular marker provided by the invention can be used for breeding pheasants, and can be used for retaining individuals with favorable genotypes and eliminating individuals with unfavorable genotypes so as to improve the body weight characteristics of the population, thereby achieving the purpose of improving the growth performance of the population and further accelerating the breeding process of the pheasants.

Inventors

  • ZHANG WENGANG
  • XUE YUN
  • LIU KE
  • PAN JUAN
  • GU XIN
  • WANG ZHIBO
  • QI LINA
  • LI XIANYU
  • HUANG SHIXIN
  • XING LEI
  • YUAN HONGYAN
  • ZHANG WEIYI
  • ZHOU JIN
  • WU HAOMIN

Assignees

  • 上海市动物疫病预防控制中心(上海市兽药饲料检测所、上海市畜牧技术推广中心)

Dates

Publication Date
20260512
Application Date
20260226

Claims (9)

  1. 1. A molecular marker, characterized in that based on genomic version ASM414374v1, the molecular marker comprises any one or more of ：NW_022205466.1:1015314、NW_022205466.1:1015332、NW_022205466.1:1015344、NW_022205466.1:1018235、NW_022205466.1:1018236、NW_022205466.1:1007748、NW_022205466.1:1007756、NW_022205466.1:1007763 and nw_022205466.1:1041908; the polymorphism of NW_022205466.1:1015314 is T or C; the polymorphism of NW_022205466.1:1015332 is T or C; The polymorphism of NW_022205466.1:1015344 is C or T; The polymorphism of NW_022205466.1:1018235 is C or T; the polymorphism of NW_022205466.1:1018236 is A or G; the polymorphism of NW_022205466.1:1007748 is G or A; the polymorphism of NW_022205466.1:1007756 is C or T; the polymorphism of NW_022205466.1:1007763 is G or T; the polymorphism of NW_022205466.1:1041908 is T or C.
  2. 2. The molecular marker combination according to claim 1, wherein, The nucleotide sequence of NW_022205466.1:1015314 is shown as SEQ ID NO. 1; The nucleotide sequence of NW_022205466.1:1015332 is shown as SEQ ID NO. 2; The nucleotide sequence of NW_022205466.1:1015344 is shown in SEQ ID NO. 3; the nucleotide sequence of NW_022205466.1:1018235 is shown as SEQ ID NO. 4; the nucleotide sequence of NW_022205466.1:1018236 is shown as SEQ ID NO. 5; the nucleotide sequence of NW_022205466.1:1007748 is shown in SEQ ID NO. 6; the nucleotide sequence of NW_022205466.1:1007756 is shown as SEQ ID NO. 7; the nucleotide sequence of NW_022205466.1:1007763 is shown in SEQ ID NO. 8; the nucleotide sequence of NW_022205466.1:1041908 is shown in SEQ ID NO. 9.
  3. 3. A primer pair is characterized in that, the primer pair is used for amplifying the molecular marker according to claim 1 or 2.
  4. 4. A kit comprising the molecular marker of claim 1 or 2, or the primer pair of claim 3.
  5. 5. Use of a molecular marker according to claim 1 or 2 as a target in any of the following: (1) Predicting or detecting the weight of pheasants, or preparing a reagent for predicting or detecting the weight of pheasants; (2) Identifying or breeding high weight pheasants, or preparing reagents for identifying or breeding high weight pheasants; (3) Molecular marker assisted breeding of pheasants; (4) Improving pheasant varieties related to weight; (5) Improving germplasm resources of pheasants.
  6. 6. Use of the molecular marker of claim 2, or the primer pair of claim 3, or the kit of claim 4, in any of the following: (1) Predicting or detecting the weight of pheasants, or preparing a reagent for predicting or detecting the weight of pheasants; (2) Identifying or breeding high weight pheasants, or preparing reagents for identifying or breeding high weight pheasants; (3) Molecular marker assisted breeding of pheasants; (4) Improving pheasant varieties related to weight; (5) Improving germplasm resources of pheasants.
  7. 7. A method of identifying body weight of a pheasant comprising: detecting the polymorphism of the molecular marker as set forth in claim 1 or 2 on the pheasants to be detected, and judging the weight of the pheasants to be detected according to genotype detection results.
  8. 8. The method of claim 7, wherein the method of detection comprises one or more of gene sequencing, molecular probes, liquid phase capture, or mass spectrometry.
  9. 9. The method of claim 7 or 8, wherein determining the weight of the test pheasant based on the genotype test results comprises: The genotype CC of NW_022205466.1:1015314 is greater than CT > TT according to the weight from big to small; genotype CC > CT > TT of NW_022205466.1:1015332; genotype TT > TC > CC of NW_022205466.1:1015344; genotype TT > TC > CC of NW_022205466.1:1018235; Genotype GG > GA > AA of NW_022205466.1:1018236; genotype AG > GG of NW_022205466.1:1007748; Genotype TC > CC of NW_022205466.1:1007756; genotype TG > GG of NW_022205466.1:1007763; genotype CC > CT > TT of NW_022205466.1:1041908.

Description

Molecular marker related to pheasant weight traits and application thereof Technical Field The invention relates to the technical field of animal breeding, in particular to a molecular marker related to pheasant weight characteristics and application thereof. Background Pheasant breeding is an important component of special poultry breeding. However, for a long time, the pheasant industry has the problems of more mixed variety sources, low system breeding degree, unhealthy fine variety breeding system and the like, which restricts the further development of the industry to a certain extent. In order to improve the productivity of pheasants, some breeding institutions bred excellent varieties with meat and egg performances by crossing and systematically breeding the pheasants with different sources (such as certain introduced varieties and local varieties). These new varieties generally exhibit stable genetic traits and well-ordered appearance and body shapes. Compared with common pheasants which are not bred systematically, the production performance of the common pheasants is obviously improved, for example, the growth speed is faster, the meat performance is better, the egg laying performance is better, the variety performance uniformity is high, considerable comprehensive benefits are brought to breeding, and the wide application prospect is shown. With the improvement of the social living standard, the demands of consumers on high-quality and special poultry products are increasing, and a wide market space is provided for pheasant varieties with excellent performances. Among the numerous economic traits of pheasants, the body weight trait is a core index reflecting the growth performance of the pheasants, and genetic improvement of the trait is directly related to the economic benefit of cultivation. In particular, the body weight trait in the later stages of production plays a critical role in pheasant breeding. On one hand, the physical development of the pheasants in this period is mature, the weight of the pheasants can comprehensively reflect the physical energy storage, fat deposition and skeletal development conditions of individuals, and on the other hand, the characteristics are closely related to a plurality of reproductive performance indexes such as the duration of egg laying peak, eggshell quality, final elimination value of the pheasants and the like, so that the pheasants are important indexes for comprehensively evaluating the later production performance. However, when breeding such traits as body weight in the later production period, the conventional breeding methods such as conventional family breeding have the limitations of low improvement efficiency, long breeding period and the like, and are difficult to efficiently meet the urgent demands of the market on high-yield and stable-yield pheasant varieties. Thus, there is a need in the industry to identify molecular markers that are closely related to key economic traits (such as body weight) and to use these markers to develop molecular marker-assisted selection or genomic selection. Through the modern biotechnology means, the breeding period can be obviously shortened, and the target characters can be precisely and efficiently bred, so that the overall efficiency of pheasant breeding work is effectively improved. Disclosure of Invention In order to solve the problems in the prior art, the invention provides a molecular marker related to the weight character of pheasants and application thereof. In a first aspect, the invention provides a molecular marker comprising any one or more of ：NW_022205466.1:1015314、NW_022205466.1:1015332、NW_022205466.1:1015344、NW_022205466.1:1018235、NW_022205466.1:1018236、NW_022205466.1:1007748、NW_022205466.1:1007756、NW_022205466.1:1007763 and nw_022205466.1:1041908, based on genomic version ASM414374v 1; the polymorphism of NW_022205466.1:1015314 is T or C; the polymorphism of NW_022205466.1:1015332 is T or C; The polymorphism of NW_022205466.1:1015344 is C or T; The polymorphism of NW_022205466.1:1018235 is C or T; the polymorphism of NW_022205466.1:1018236 is A or G; the polymorphism of NW_022205466.1:1007748 is G or A; the polymorphism of NW_022205466.1:1007756 is C or T; the polymorphism of NW_022205466.1:1007763 is G or T; the polymorphism of NW_022205466.1:1041908 is T or C. Further, the nucleotide sequence of NW_022205466.1:1015314 is shown in SEQ ID NO. 1; The nucleotide sequence of NW_022205466.1:1015332 is shown as SEQ ID NO. 2; The nucleotide sequence of NW_022205466.1:1015344 is shown in SEQ ID NO. 3; the nucleotide sequence of NW_022205466.1:1018235 is shown as SEQ ID NO. 4; the nucleotide sequence of NW_022205466.1:1018236 is shown as SEQ ID NO. 5; the nucleotide sequence of NW_022205466.1:1007748 is shown in SEQ ID NO. 6; the nucleotide sequence of NW_022205466.1:1007756 is shown as SEQ ID NO. 7; the nucleotide sequence of NW_022205466.1:1007763 is shown in SEQ ID NO. 8; the nucleotide sequ