CN-122012740-A - Application of double type high-association SNP locus of puffer green in breeding

Abstract

The invention discloses an application of double-type high-association SNP loci of Fugu green in breeding, and belongs to the technical field of aquatic breeding. By analyzing the pre-sequencing data of the W group and the M group of the puffer fish, two linkage disequilibrium SNP loci at a position 269 and a position 469 of MYF6 genes with higher genetic diversity are identified, three haplotypes are detected in the W group and the M group together, and five double types are randomly formed by the three haplotypes, wherein the homozygous double V type (G 269 G 269 C 469 C 469 ) has obvious advantages in terms of average height and MYF6 gene expression compared with other four double types, can be used as a high-related molecular marker, and is applied to molecular assisted breeding of a new line of puffer fish for improving the yield of the improved variety of puffer fish.

Inventors

• WANG KAI
• Lv Wuhong
• LIU QIANGDE
• LI MINGZHU
• TANG YU
• SU XIAOLEI
• QU XUEWEI
• HOU CHUNYU
• HOU YUPING
• KONG QING
• YANG TAO
• LI ZHONGHU
• LIU CONG

Assignees

• 鲁东大学
• 烟台市牟平区渔业技术推广站
• 烟台市海洋经济研究院(烟台市渔业技术推广站、烟台市海洋捕捞增殖管理站)
• 烟台普胜水产有限公司

Dates

Publication Date

20260512

Application Date

20260316

Claims (1)

1. 1. The application of the homozygous double V type of the high-association SNP locus of the puffer fish in molecular assisted breeding of the quick-growing new strain of the puffer fish, which is characterized in that the double type is randomly formed by a single type I, a single type II and a single type III, wherein bases at a position 269 and a position 469 of a MYF6 gene of the single type I are A, A respectively, bases at a position 269 and a position 469 of the single type II are A, C respectively, bases at a position 269 and a position 469 of the MYF6 gene of the single type III are G, C respectively, the nucleotide sequence of the MYF6 gene is shown as SEQ ID NO:1 or SEQ ID NO:2, and three single types randomly form five types of homozygous double type I A 269 A 269 A 469 A 469 , heterozygous double type II A 269 A 269 A 469 C 469 , homozygous double type III A 269 A 269 C 469 C 469 , heterozygous double type IV A 269 G 269 C 469 C 469 and homozygous double V type G 269 G 269 C 469 C 469 respectively, and the homozygous double V type has obvious advantages in terms of average gene expression compared with other four types of MYF 6.

Description

Application of double type high-association SNP locus of puffer green in breeding Technical Field The invention relates to an application of double-type high-association SNP loci of puffer fish in breeding, belonging to the technical field of aquatic breeding. Background The body height is an important index for evaluating the growth performance of the puffer fish. At present, as the parent of the puffer fish mainly comes from wild fishing, the control of germplasm resources is lacking, and the unordered selfing causes the obvious decline of the growth performance of the cultivated species of the puffer fish, thereby greatly restricting the development of industrial cultivation of the puffer fish. At present, china lacks an artificially cultivated excellent puffer fish culture strain. The research team screens out the quick-growing population of the Fugu obscurus from the existing culture population of the Fugu obscurus, and positions high-association SNP loci so as to improve the culture yield of the Fugu obscurus. At present, no application of high-association SNP loci of the puffer fish in breeding exists, and the most similar SNP molecular marker related to the growth trait of the puffer fish and the application thereof (CN 117925860A) are disclosed. However, the patent only identifies that single base mutation is related to the growth of the puffer fish, and does not establish linkage disequilibrium relationship between the body height and the genotype, so that the patent has great limitation in practical production. Disclosure of Invention In order to solve the defects of the prior art, the invention aims to provide the application of double-type high-association SNP loci of the Fugu similis in breeding so as to overcome the limitation of various factors, strictly regulate and control the growth process of the Fugu similis and promote the molecular assisted breeding of quick-growing new lines of the Fugu similis. In order to achieve the above object, the present invention adopts the following technical scheme: The application of the homozygous double V type of the high-association SNP locus of the puffer fish in molecular assisted breeding of a quick-growing new strain of the puffer fish, wherein the double type is randomly formed by a single type I, a single type II and a single type III, wherein bases at a position 269 and a position 469 of a MYF6 gene of the single type I are A, A respectively, bases at a position 269 and a position 469 of the single type II are A, C respectively, bases at a position 269 and a position 469 of the MYF6 gene of the single type III are G, C respectively, the nucleotide sequence of the MYF6 gene is shown as SEQ ID NO:1 or SEQ ID NO:2, and three single types randomly form five double types of homozygous double type I A 269A269A469A469, heterozygous double type II A 269A269A469C469, homozygous double type III A 269A269C469C469, heterozygous double type IV A 269G269C469C469 and homozygous double type V type G 269G269C469C469 respectively, and the homozygous double type V type has obvious advantages in terms of average high and F6 gene expression compared with other four double types. The invention has the advantages that 2 SNP loci of the puffer fish flesh engendering factor 6 (MYF 6) gene (which are respectively positioned at 269 and 469 of the MYF6 gene) are subjected to correlation analysis with the puffer fish body height, and the 2 SNP loci are found to be obviously related to the puffer fish body height, wherein single III type (G 269C469) individuals are higher. Through further high contrast analysis of five types of individuals, namely homozygous double type I (DI), heterozygous double type II (DII), homozygous double type III (DIII), heterozygous double type IV (DIV) and homozygous double type V (DV), the expression of the high and MYF6 genes of the Fugu similis is found to have significant differences between fast-growing populations and normal populations, wherein the relative expression quantity of the high and MYF6 genes of the Fugu similis in the homozygous double type V (DV) is significantly higher than that of other four types of double types, and the double type V (DV) can be used as a high-correlation molecular marker to be applied to molecular assisted breeding of the quick-growing new variety of the Fugu similis so as to improve the yield of the quick-growing fine variety of the Fugu similis. Drawings Fig. 1 is a high contrast plot of the average and fast-growing populations of puffer fish for 1-5 months of age, representing P <0.05; FIG. 2 is a graph showing the relative expression levels of MYF6 genes of five kinds of double-type 5 months of age of Fugu green. Detailed Description The present invention will be described in detail with reference to the drawings and examples. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the examples described below, unless otherwise s