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CN-122012801-A - KASP molecular marker linked with first female flower node position character of balsam pear and application thereof

CN122012801ACN 122012801 ACN122012801 ACN 122012801ACN-122012801-A

Abstract

The invention discloses a KASP molecular marker linked with the first female flower node position character of balsam pear and application thereof, wherein the KASP molecular marker is positioned at 22054848 position of chromosome 4 of balsam pear genome, and the base at the position is A or C. The KASP molecular marker and the amplification primer thereof can be used for effectively detecting the first female flower node position character of the balsam pear, are favorable for screening low first female flower node position balsam pear varieties, and have important guiding significance for auxiliary breeding of the balsam pear molecular marker.

Inventors

  • LIU XIAOQIAN
  • DENG LITING
  • WANG XUETING
  • GONG HAO
  • HE MING
  • ZHENG XIAOMING
  • ZHENG YANGYI
  • WU HAIBIN
  • LI JUNXING
  • LUO JIANNING
  • ZHAO GANGJUN

Assignees

  • 广东省农业科学院蔬菜研究所

Dates

Publication Date
20260512
Application Date
20260413

Claims (10)

  1. 1. An application of a KASP molecular marker linked with the first female node position character of a balsam pear in detecting the first female node position character of the balsam pear or screening low first female node position balsam pear germplasm resources, wherein the KASP molecular marker is positioned at 22054848 position of chromosome 4 of a balsam pear genome, and the base is A or C.
  2. 2. The amplification primer of the KASP molecular marker linked with the first female flower node position character of the balsam pear is characterized by comprising an upstream primer F1 with a sequence shown as SEQ ID NO. 1, an upstream primer F2 with a sequence shown as SEQ ID NO. 2 and a downstream primer R with a sequence shown as SEQ ID NO. 3.
  3. 3. The amplification primer of the KASP molecular marker linked to the first female node trait of balsam pear according to claim 2, wherein the 5 'end of the upstream primer F1 is modified with a FAM group and the 5' end of the upstream primer F2 is modified with a HEX group.
  4. 4. Use of an amplification primer of a KASP molecular marker linked to the first female node trait of a balsam pear according to claim 2 or 3 for detecting the first female node trait of a balsam pear or for screening low first female node balsam pear germplasm resources.
  5. 5. Use of an amplification primer of a KASP molecular marker linked to the first female node trait of bitter gourd according to claim 2 or 3, in the preparation of a kit for detecting the first female node trait of bitter gourd or for screening low first female node germplasm resources.
  6. 6. A kit for detecting a first female node trait of a balsam pear or for screening germplasm resources of a balsam pear with a low first female node, comprising the amplification primer of the KASP molecular marker linked to the first female node trait of a balsam pear according to claim 2 or 3.
  7. 7. A method for detecting the first female node position character of balsam pear is characterized by comprising the following steps of taking DNA of balsam pear to be detected as a template, carrying out PCR amplification by using the amplification primer as set forth in claim 2 or 3, and analyzing genotyping data.
  8. 8. The method for detecting the first female node position character of the balsam pear according to claim 7, wherein the analysis of the genotyping data comprises the steps of determining that the balsam pear to be detected is a high first female node balsam pear variety when the genotype is CC and determining that the balsam pear to be detected is a low first female node balsam pear variety when the genotype is AA or AC.
  9. 9. A method for screening low first female flower node balsam pear germplasm resources is characterized by comprising the following steps of taking DNA of balsam pear to be detected as a template, carrying out PCR amplification by using the amplification primer of claim 2 or 3, and screening balsam pear germplasm resources with AA or AC genotype.
  10. 10. The method according to any one of claims 7 to 9, wherein the reaction system for PCR amplification comprises 2 xKASP master mix 5.0 + -0.1. Mu.L, 8. Mu.M-10. Mu.M upstream primer F1.15+ -0.01. Mu.L, 8. Mu.M-12. Mu.M upstream primer F2.15+ -0.01. Mu.L, 10. Mu.M downstream primer R0.4+ -0.05. Mu. L, DNA template 10 ng-100 ng, ddH 2 O added to 10. Mu.L; the PCR amplification reaction program comprises 94 ℃ and 15 min, 94 ℃ and 20 s, 65-57 ℃ and 1 min and 10 cycles, and 94 ℃ and 20 s and 57 ℃ and 1 min and 30 cycles.

Description

KASP molecular marker linked with first female flower node position character of balsam pear and application thereof Technical Field The invention belongs to the technical field of crop genetic breeding, and particularly relates to a KASP molecular marker closely linked with a first female flower node QTL site of balsam pear and application thereof. Background Balsam pear (Momordica charantia l.) is an important economic vegetable of the cucurbitaceae family, planted in tropical and subtropical asia areas, and is known for its characteristic bitter taste and remarkable medicinal value. Flowering time is a critical turning point in the plant development process, marks the transition of plants from vegetative to reproductive growth, and significantly affects crop fitness, regional distribution, and final yield. The first female node is a key agronomic trait that determines the precocity of cucurbitaceae crops, which directly affects female differentiation time, early yield and harvest time. Genetic control mechanisms with respect to flowering time and first female node position have been widely studied in arabidopsis and various vegetable crops. Lower female node position generally means earlier flowering and fruit setting, helps plants to circumvent later biotic and abiotic stress, shortens production cycle, and reduces cultivation cost. Currently, the available molecular markers closely linked to the first female node of balsam pear are very limited. Disclosure of Invention Based on this, the present invention aims to provide a molecular KASP marker closely linked to the first female node trait of Momordica charantia. The specific technical scheme for achieving the aim of the invention comprises the following steps. In a first aspect, the invention provides an application of a KASP molecular marker linked with a first female node position character of a balsam pear in detecting the first female node position character of the balsam pear or screening a balsam pear germplasm resource with low first female node position, wherein the KASP molecular marker is positioned at 22054848 position of a No. 4 chromosome of a balsam pear genome, and a base at the position is A or C. In a second aspect of the invention, a KASP molecular marker amplification primer linked with the first female flower node position character of balsam pear is provided, which comprises an upstream primer F1 with a sequence shown as SEQ ID NO. 1, an upstream primer F2 with a sequence shown as SEQ ID NO. 2 and a downstream primer R with a sequence shown as SEQ ID NO. 3. In a third aspect, the invention provides an application of the amplification primer of the KASP molecular marker linked with the first female node position character of the balsam pear in detecting the first female node position character of the balsam pear or screening low first female node position balsam pear germplasm resources. In a fourth aspect, the invention provides an application of the amplification primer of the KASP molecular marker linked with the first female node position character of the balsam pear in preparing a kit for detecting the first female node position character of the balsam pear or screening low first female node position balsam pear germplasm resources. In a fifth aspect of the invention, a kit for detecting the first female node position trait of bitter gourd or screening low first female node position bitter gourd germplasm resources is provided, which comprises the KASP molecular marker amplification primer linked with the first female node position trait of bitter gourd. In a sixth aspect of the invention, a method for detecting the node position of a first female flower of a balsam pear is provided, which comprises the steps of taking DNA of the balsam pear to be detected as a template, carrying out PCR amplification by using the amplification primer, and analyzing genotyping data. In a seventh aspect, the invention provides a method for screening low-first female-flower node balsam pear germplasm resources, which comprises the following steps of taking DNA of balsam pear to be detected as a template, carrying out PCR amplification by using the amplification primer, and screening balsam pear germplasm resources with AA or AC genotype. The inventor of the invention takes a balsam pear resource with low first female flower node position and an F 2 separation group created by balsam pear resource with high first female flower node position as research objects, finds a SNP locus variation A/C at a 22054848 position of a No.4 chromosome of the balsam pear, wherein the SNP locus is a KASP molecular marker closely linked with the first female flower node position character, A is closely linked with the low first female flower node position character, C is closely linked with the high first female flower node position character, and further designs an amplification primer according to the KASP molecular marker, and experiments prove that the KASP mol