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CN-122012805-A - Molecular marker related to oil yield of apocarya and application thereof

CN122012805ACN 122012805 ACN122012805 ACN 122012805ACN-122012805-A

Abstract

The invention discloses a group of molecular markers related to the oil yield of apocarya and application thereof, and relates to the technical field of biology. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO. 1-2. The molecular marker can be used for molecular marker assisted selection (MARKER ASSISTED selection, MAS) and genome selection (Genomic selection, GS) of apocarya, and can accelerate the genetic improvement progress of apocarya varieties.

Inventors

  • ZHANG FAN
  • ZHANG JIYU
  • LI XINZHI
  • SUN YULUN
  • WANG JIAJIA

Assignees

  • 江苏省中国科学院植物研究所

Dates

Publication Date
20260512
Application Date
20260415

Claims (6)

  1. 1. The molecular marker sequence related to the oil yield of apocarya is characterized by comprising at least one of SEQ ID NO. 1-SEQ ID NO.2, wherein the polymorphic site of SEQ ID NO.1 corresponds to the G > A mutation at position 301 of SEQ ID NO.1, AA is a genotype with high oil yield, GG is a genotype with low oil yield, and the polymorphic site of SEQ ID NO.2 corresponds to the C > T mutation at position 301 of SEQ ID NO.2, CC and/or CT are genotypes with high oil yield, and TT is a genotype with low oil yield.
  2. 2. The primer group for detecting the molecular marker is characterized in that the nucleotide sequence of the primer group is shown as SEQ ID NO. 3-SEQ ID NO.6, wherein SEQ ID NO. 3-SEQ ID NO.4 is used for detecting a molecular marker SNP1, SEQ ID NO. 5-SEQ ID NO.6 is used for detecting a molecular marker SNP2, the molecular marker SNP1 corresponds to the 301 th G > A mutation of the SEQ ID NO.1, the nucleotide sequence is shown as SEQ ID NO.1, wherein AA is a genotype with high oil yield, GG is a genotype with low oil yield, the SNP2 corresponds to the 301 th C > T mutation of the SEQ ID NO.2, the nucleotide sequence is shown as SEQ ID NO.2, CC and/or CT is a genotype with high oil yield, and TT is a genotype with low oil yield.
  3. 3. A kit for detecting a molecular marker, characterized in that the kit comprises the primer set according to claim 2.
  4. 4. A method for evaluating the oil yield traits of apocarya, comprising the steps of: (1) Extracting genome DNA of a to-be-detected apocarya sample; (2) Detecting the genotype of the apocarya to be detected by adopting the primer set as defined in claim 2 or the kit as defined in claim 3; (3) Determining the genotype of the apocarya to be detected based on the detection result; (4) And evaluating the oil yield of apocarya according to the genotype result obtained by detection, wherein the genotype individual with any locus with the evaluation criterion of the genotype individual with the AA type SNP1 locus and the genotype individual with the CC or CT type SNP2 locus is evaluated, the SNP1 corresponds to the 301 th G & gtA mutation of SEQ ID NO.1, the nucleotide sequence is shown as SEQ ID NO.1, the SNP2 corresponds to the 301 th C & gtT mutation of SEQ ID NO.2, and the nucleotide sequence is shown as SEQ ID NO. 2.
  5. 5. A genetic breeding method for improving the oil yield traits of apocarya, which is characterized by comprising the following steps: (1) Extracting genome DNA of a pecan resource group to be detected; (2) Detecting the genotype of the apocarya to be detected by adopting the primer set as defined in claim 2 or the kit as defined in claim 3; (3) Determining the genotype of the apocarya to be detected based on the detection result; (4) According to the breeding target, selecting individuals with different genotypes as parents, and when the breeding target is to select a variety with high oil yield, selecting the following genotype individuals as parents: The SNP1 locus is an AA type individual and/or the SNP2 locus is a CC or CT type individual, wherein the SNP1 corresponds to the 301 th G > A mutation of SEQ ID NO.1, the nucleotide sequence of the SNP1 is shown as SEQ ID NO.1, the SNP2 corresponds to the 301 th C > T mutation of SEQ ID NO.2, and the nucleotide sequence of the SNP2 is shown as SEQ ID NO. 2.
  6. 6. Use of the primer set of claim 2 or the kit of claim 3 in the performance-related breeding of apocarya or the prediction of apocarya oil yield.

Description

Molecular marker related to oil yield of apocarya and application thereof Technical Field The invention belongs to the technical field of biology, and particularly relates to a molecular marker related to oil yield of apocarya and application thereof. Background The apocarya (Carya illinoensis) is taken as a world famous dry fruit oil tree species, the kernel oil content of the apocarya is up to 51% -69%, the oil and fat mainly contains unsaturated fatty acids such as oleic acid, linoleic acid and the like, the total amount of the unsaturated fatty acids is more than 90%, and the apocarya is an important source of high-quality high-grade edible oil and has remarkable economic value. The oil yield is used as the key economic character of the apocarya, directly determines the development value and market competitiveness of the apocarya for oil, and the variety with high oil yield plays a dominant role in improving the economic benefit of oil processing and industry, thereby being a key requirement for high-quality development of industry. However, the current apocarya oil yield evaluation and oil breeding field has outstanding technical bottlenecks that on one hand, the traditional oil yield evaluation depends on physical and chemical detection after fruit ripening, the traditional oil yield evaluation can be implemented by waiting for a complete growth period of plants to a fruiting period, the seedling period can not judge the oil yield related genotype through phenotype, so that a large number of plants need to be cultivated synchronously in breeding, and after fruiting, target individuals are screened, so that resources such as land, manpower and the like are seriously wasted, and on the other hand, the traditional detection method is complex in operation, time-consuming and labor-consuming, high in cost, easy to be interfered by factors such as planting environment, maturity and the like, and poor in stability. On the breeding level, traditional breeding relies on phenotype selection, multiple generations of screening and verification are needed for breeding aiming at oil yield, the period is long, the efficiency is low, and urgent requirements of industries on high-oil varieties are difficult to meet. Modern technologies such as molecular marker assisted breeding (MAS), genome Selection (GS) and the like become important means for crop genetic improvement, and SNP molecular markers have remarkable advantages due to the characteristics of wide distribution, high polymorphism, accurate detection and the like. The prior researches develop SNP markers aiming at the characters of phenols, flowering types and the like of apocarya, and also measure the oil content and fatty acid composition of partial varieties, but specific SNP molecular markers closely related to the oil yield are not found yet, and the accurate molecular markers and a matched detection system which can be directly applied to breeding practice are lacking. The existing molecular markers can not meet the requirements of directional breeding of oil yield, and the GS model is insufficient in prediction precision due to the lack of core character marker support, so that the genetic improvement process of the oil-used apocarya is restricted. In addition, in industrial scenes such as breeding selection, seedling identification, resource evaluation and the like, rapid and accurate oil yield evaluation technology is lacked, so that high-quality seedling screening efficiency is low, and industrial development quality is affected. Therefore, the SNP molecular marker related to the oil yield of apocarya is developed, a high-efficiency detection system is constructed, and the SNP molecular marker is applied to molecular marker assisted selection (MARKER ASSISTED selection, MAS) breeding and genome selection (Genomic selection, GS) model construction, so that the seedling stage precise prediction and directional breeding are realized, and the method has important significance in reducing the breeding cost, accelerating the cultivation of high-oil seeds and promoting the development of industry high quality. Disclosure of Invention In order to solve the technical problems, the invention develops a set of molecular markers for evaluating the oil yield of the apocarya. The SNP molecular marker for evaluating the oil yield of the apocarya is developed through a high-throughput sequencing technology and verified in genetic groups and natural groups, and the marker can be used for commercial identification of apocarya (seedlings) sold in the market, and can accelerate the genetic improvement progress of apocarya varieties when being applied to MAS breeding and GS model construction. The primary purpose of the invention is to determine a molecular marker related to the oil yield performance of the apocarya, wherein the molecular marker is positioned on the genome of the apocarya (self sequencing assembly of the subject group), and specific SNP information is as