Search

CN-122013369-A - Method for directly preparing chitin nanofiber from shrimp and crab shells based on eutectic solvent system and chitin nanofiber

CN122013369ACN 122013369 ACN122013369 ACN 122013369ACN-122013369-A

Abstract

The application relates to a method for directly preparing chitin nanofibers by shrimp and crab shells based on a eutectic solvent system and the chitin nanofibers. A method for directly preparing chitin nanofibers from shrimp and crab shells based on a eutectic solvent system comprises the steps of mixing a hydrogen bond donor and a hydrogen bond acceptor to obtain the eutectic solvent system, reacting shrimp and crab shell powder with the eutectic solvent system at 40-100 ℃ for 2-6h and stirring speed of 200-500r/min, performing first ultrasonic treatment under the conditions of power of 800-2400W, frequency of 20-25kHz and total ultrasonic time of 30-180 min, and separating, washing and performing second ultrasonic treatment after the reaction is finished to obtain the chitin nanofibers. The application can synchronously realize three processes of deproteinization, demineralization and chitin nanocrystallization in the same reaction system, simplifies the process flow, reduces the energy consumption and the environmental pollution, and improves the yield and the quality of the chitin nanofiber.

Inventors

  • ZHAO YAN
  • ZHANG WENCHANG
  • AN GUOQING

Assignees

  • 海湾(佛山)生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260331

Claims (10)

  1. 1. The method for directly preparing the chitin nanofiber by using the shrimp and crab shells based on the eutectic solvent system is characterized by comprising the following steps of: The hydrogen bond donor and the hydrogen bond acceptor are mixed according to the molar ratio of 1:1-3:1 to prepare a eutectic solvent system; The shrimp and crab shell powder reacts with the eutectic solvent system at the reaction temperature of 40-100 ℃, the reaction time of 2-6h and the stirring rate of 200-500 r/min; The first ultrasonic treatment is carried out under the conditions that the ultrasonic power is 800-2400W, the ultrasonic frequency is 20-25kHz, the pulse ultrasonic mode is 2s-5s of ultrasonic, the intermittent ultrasonic mode is 2s-4s, the system temperature is controlled to be less than 70 ℃, and the total ultrasonic time is 30min-180min; after the reaction is finished, separating, washing and performing ultrasonic treatment for the second time to obtain the chitin nanofiber.
  2. 2. The method for directly preparing chitin nanofibers according to claim 1, wherein the mass ratio of said shrimp and crab shell powder to said eutectic solvent system is 1 (8-20).
  3. 3. The method for directly preparing chitin nanofibers based on shrimp and crab shells of eutectic solvent system according to claim 1, wherein said second ultrasonic treatment is the same as said first ultrasonic treatment.
  4. 4. The method for directly preparing chitin nanofibers by shrimp and crab shells based on a eutectic solvent system according to claim 1, wherein the hydrogen bond donor comprises one or more of lactic acid, citric acid, acetic acid, formic acid and malic acid, and the hydrogen bond acceptor comprises quaternary ammonium salt compounds.
  5. 5. The method for directly preparing chitin nanofibers by shrimp and crab shells based on eutectic solvent system according to claim 4, wherein said quaternary ammonium salt compound comprises choline chloride and betaine.
  6. 6. The method for directly preparing chitin nanofibers according to claim 1, wherein fresh shrimp and crab shells are used as raw materials, sediment, meat residue and impurities attached to the surfaces of the fresh shrimp and crab shells are removed, the shrimp and crab shells are washed clean by clean water, and the shrimp and crab shells are dried to constant weight at low temperature, so that the shrimp and crab shell powder is obtained.
  7. 7. The method for directly preparing chitin nanofibers by shrimp and crab shells based on eutectic solvent system according to claim 6, wherein the low temperature drying temperature is 60-80 ℃.
  8. 8. The method for directly preparing chitin nanofibers according to claim 6, wherein said shrimp and crab shell powder is obtained by drying at low temperature to constant weight and pulverizing to 100-200 mesh powder.
  9. 9. A chitin nanofiber prepared by a method for directly preparing the chitin nanofiber based on the shrimp and crab shells based on the eutectic solvent system according to any one of claims 1-8.
  10. 10. The chitin nanofiber according to claim 9, characterized in that the diameter is 20-60nm.

Description

Method for directly preparing chitin nanofiber from shrimp and crab shells based on eutectic solvent system and chitin nanofiber Technical Field The application relates to the field of biomass nano material preparation, and mainly relates to a method for directly preparing chitin nano fibers from shrimp and crab shells based on a eutectic solvent system and the chitin nano fibers. Background Chitin is a natural polymer material widely existing in shells of crustaceans such as shrimps and crabs, has excellent biocompatibility, biodegradability and bioactivity, and a nanocrystallization product, namely chitin nanofiber, further extends the core advantages, has wide application prospects in various fields such as biological medicine, food, cosmetics and environmental protection, and also becomes an important direction of current chitin application research. The traditional chitin nanofiber preparation method generally comprises three core steps of deproteinizing, namely treating shrimp and crab shells by adopting an acid-base solution to remove protein components, demineralizing, stripping mineral substances mainly containing calcium carbonate from the shells by adopting a strong acid solution, and further refining the chitin microfibers into nanofibers by means of mechanical grinding, high-pressure homogenization or chemical treatment and the like. Although the method can successfully prepare the chitin nanofiber, the preparation method has the outstanding problems that the preparation process flow is complicated, the production period is prolonged, the production cost is obviously increased due to the fact that multiple steps are required to be independently operated, meanwhile, the reaction condition is relatively harsh, the steps are carried out under high-temperature and high-pressure environments, the energy consumption is large, the development requirements of current energy conservation and consumption reduction are not met, in addition, the environmental protection pressure is outstanding, a large amount of strong acid and strong alkali are required to be used in the preparation process, a large amount of wastewater and waste residues are generated, the subsequent treatment cost is high, serious pollution is easily caused to the ecological environment, in addition, the product yield is relatively low, a large amount of chitin is easily lost in the multiple steps of operation, in addition, the product quality stability is insufficient, the molecular structure of the chitin is easily damaged due to the multiple steps of complex operation, and the performance of core products such as biological activity is further influenced. Therefore, the development of the preparation method which is efficient, energy-saving and environment-friendly and can improve the yield and the product quality is important to promote the industrialized development of the chitin nanofiber and fully release the application value of the chitin nanofiber. Disclosure of Invention In view of the shortcomings of the prior art, the application aims to provide a method for directly preparing chitin nanofibers by shrimp and crab shells based on a eutectic solvent system and the chitin nanofibers, and aims to synchronously realize three processes of deproteinization, demineralization and chitin nanocrystallization in the same reaction system, simplify the process flow, reduce energy consumption and environmental pollution and improve the yield and quality of the chitin nanofibers. The technical scheme of the application is as follows: A method for directly preparing chitin nanofibers from shrimp and crab shells based on a eutectic solvent system comprises the following steps: The hydrogen bond donor and the hydrogen bond acceptor are mixed according to the molar ratio of 1:1-3:1 to prepare a eutectic solvent system; The shrimp and crab shell powder reacts with the eutectic solvent system at the reaction temperature of 40-100 ℃, the reaction time of 2-6h and the stirring rate of 200-500 r/min; the first ultrasonic treatment is carried out under the conditions that the ultrasonic power is 800-2400W, the ultrasonic frequency is 20-25kHz, the pulse ultrasonic mode is 2s-5s of ultrasonic, the intermittent ultrasonic mode is 2s-4s, the system temperature is controlled to be less than 70 ℃, and the total ultrasonic time is 30min-180min; after the reaction is finished, separating, washing and performing ultrasonic treatment for the second time to obtain the chitin nanofiber. Further, the second sonication is the same as the first sonication. Further, the mass ratio of the shrimp and crab shell powder to the eutectic solvent system is 1 (8-20). Further, the hydrogen bond donor comprises one or more than two of lactic acid, citric acid, acetic acid, formic acid and malic acid, and the hydrogen bond acceptor comprises a quaternary ammonium salt compound. Further, the quaternary ammonium salt compound comprises choline chloride and betaine. Further, fr