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CN-122016422-A - Batch processing system and method for brain-spinal cord samples

CN122016422ACN 122016422 ACN122016422 ACN 122016422ACN-122016422-A

Abstract

The disclosure belongs to the technical field of experimental devices, and provides a batch processing system and a batch processing method for brain-spinal cord samples. The system comprises a fixing device of brain-spinal cord samples, an inner processing box and an outer processing box, wherein the fixing device comprises a base, a brain fixing groove and a spinal cord fixing groove which are arranged on the base, the two grooves are communicated, a frame is arranged in the inner processing box and comprises a plurality of guide rails for embedding the fixing device, and the outer processing box is used for accommodating the inner processing box so as to perform unified reagent processing on the whole sample group. Methods of batch processing brain-spinal cord samples using the above system are also disclosed. The method realizes synchronous and standardized treatment of a plurality of samples by designing the modularized sample bearing unit and the reagent processing outer box. The method can process a plurality of samples simultaneously, greatly improves the processing efficiency, ensures the consistency of the heights of the samples, eliminates batch errors to the maximum extent, ensures the data comparability, and reduces human intervention and injury to the maximum extent.

Inventors

  • REN MIAO
  • LI XIANGNING
  • LUO QINGMING
  • GONG HUI

Assignees

  • 海南大学三亚研究院

Dates

Publication Date
20260512
Application Date
20251222

Claims (10)

  1. 1. A batch processing system for brain-spinal cord samples, characterized in that the batch processing system comprises a fixing device for brain-spinal cord samples, an internal processing box and an external processing box; The fixing device comprises a base, and a brain fixing groove and a spinal cord fixing groove which are arranged on the base, wherein the brain fixing groove is communicated with the spinal cord fixing groove; the spinal cord fixing groove is a spiral bionic groove which is matched with the side anatomical outline of the spinal cord and is used for accommodating the side of the spinal cord and keeping the spinal cord at a natural connection angle with the tail end of the brainstem; a frame is arranged in the internal processing box, and comprises a plurality of guide rails for embedding a plurality of fixing devices; The external process cartridge is for accommodating the internal process cartridge to perform unified reagent processing on the entire sample group.
  2. 2. The batch processing system according to claim 1, wherein a transition arc is arranged at the joint of the brain fixing groove and the spinal cord fixing groove, the radius of the transition arc is 2-3 mm, and the transition arc is used for enabling the tail end of the brain stem of the brain to be in smooth transition connection with the starting end of the spinal cord, so that nerve fiber bending at the joint is avoided.
  3. 3. The batch processing system of claim 1, wherein the base is integrally formed with the brain fixation groove and the spinal cord fixation groove, the integrally formed method comprising any one of precision injection molding and CNC milling.
  4. 4. The batch processing system of claim 1, wherein the securing means further comprises a snap securing system disposed along a trajectory of the spinal cord securing slot for confining the spinal cord within the spinal cord securing slot, the snap securing system comprising a plurality of snaps.
  5. 5. The batch processing system of claim 4, wherein the clasp comprises a positioning support unit coupled to the base for securing the clasp to the base, an elastic restraining unit for providing elastic pressure to restrain the spinal cord within the spinal cord securing slot, and an adapter coupling unit for coupling the positioning support unit and the elastic restraining unit.
  6. 6. The batch processing system according to claim 1, wherein each of the guide rails is a groove structure arranged in parallel along a length direction of the internal processing cartridge, one fixing device corresponds to a pair of the groove structures, and both side edges of a widthwise corresponding surface of the fixing device form fit-fit engagement with a wall of the groove structure.
  7. 7. The batch processing system of claim 1, wherein the frame further comprises a limit structure that is a stop or a slot disposed at an end of the rail.
  8. 8. The batch processing system according to claim 1, wherein the bottom of the external processing box is provided with a diversion trench and a bayonet matched with the shaking table, and the cover of the external processing box is provided with a sealing ring for preventing the reagent from volatilizing.
  9. 9. A method of processing brain-spinal cord samples using the batch processing system of any one of claims 1-8, comprising the steps of: s1, fixing a plurality of brain-spinal cord samples on a fixing device of the brain-spinal cord samples; s2, embedding a fixing device for loading a sample on a guide rail of an internal processing box; S3, integrally arranging the internal processing box embedded with the fixing device in the step S2 into an external processing box, adding a chemical reagent into the external processing box, and simultaneously processing a plurality of brain-spinal cord samples; And S4, embedding, slicing, staining or three-dimensional imaging is carried out on a plurality of brain-spinal cord samples after the treatment is completed.
  10. 10. The method according to claim 9, wherein step S3 is to take out the whole internal process cartridge when the chemical reagent is replaced by performing a different process, discard the old reagent, add a new reagent directly to the external reagent cartridge, and put the internal process cartridge in.

Description

Batch processing system and method for brain-spinal cord samples Technical Field The disclosure relates to the technical field of experimental devices, in particular to a batch processing system and a batch processing method for brain-spinal cord samples. Background In neuroscience research, three-dimensional imaging and loop reconstruction of brain-spinal cord tissue of a large number of experimental animals is often required to compare differences between different groups, e.g., normal, disease model, treatment groups, in order to obtain statistically significant findings. Therefore, the preparation of a plurality of brain-spinal cord samples with high throughput and high quality is a precondition for quantitative neuro-anatomical studies. Currently, the method of total embedding of brain and spinal cord long segment tissue is basically based on a single sample manual operation mode. The typical procedure is 1. Individual handling of each ex vivo brain-spinal cord sample by the operator is required to be fixed, dehydrated, transparent and embedded, 2. Sequential handling is that due to lack of suitable bulk carrying tools, these steps can only be handled one by one or mixed placement using large containers, resulting in mutual compression and confusion of identification between samples. 3. Manual transfer-A single sample is transferred from one reagent bottle to another by hand or forceps at each processing step, e.g., changing fixative at a different concentration. The operation flow has the problems that 1. The treatment efficiency is extremely low, the time required for sequentially treating N samples is approximately N times of the time required for treating a single sample, the requirement of large-scale research cannot be met, and the experimental progress is severely restricted. 2. The processing conditions are inconsistent among samples, namely, even if the same reagents and flow paths are used, as the samples are processed sequentially, the reagent soaking time, the reagent concentration and the like of each sample in each reagent can be different, and a batch effect is introduced. 3. In the operation process, samples are easy to damage, namely, the slender spinal cord tissues are frequently clamped and transferred manually, so that the physical damage of the tissues or the falling of labels and the confusion of the samples are easily caused. 4. Reagent consumption is high, frequent reagent replacement for handling small amounts of samples, or the use of excessively large volumes of containers for holding multiple samples results in reagent waste. Therefore, an integrated system capable of realizing standardization and batch treatment of brain-spinal cord samples, including fixing, dehydration, embedding and other links is urgently needed to solve the problems of low efficiency, poor consistency, easy damage and the like. Disclosure of Invention The present disclosure provides a batch processing system and a processing method for brain-spinal cord samples, so as to at least solve the above technical problems in the prior art. According to a first aspect of the present disclosure, there is provided a batch processing system for brain-spinal cord samples, comprising a fixing device for brain-spinal cord samples, an internal processing cartridge, and an external processing cartridge; The fixing device comprises a base, and a brain fixing groove and a spinal cord fixing groove which are arranged on the base, wherein the brain fixing groove is communicated with the spinal cord fixing groove; the spinal cord fixing groove is a spiral bionic groove which is matched with the side anatomical outline of the spinal cord and is used for accommodating the side of the spinal cord and keeping the spinal cord at a natural connection angle with the tail end of the brainstem; a frame is arranged in the internal processing box, and comprises a plurality of guide rails for embedding a plurality of fixing devices; The external process cartridge is for accommodating the internal process cartridge to perform unified reagent processing on the entire sample group. Specifically, the brain fixing groove and spinal cord fixing groove integrated communication and anatomy matching design ensures that the brain and spinal cord can be maintained at a natural connection angle immediately after being isolated, the brain and spinal cord can be integrally fixed without cutting off, and the continuity of a long-range nerve loop can be ensured. The inside processing box carries out the integration to a plurality of samples that fixing device is fixed and bears, and outside processing box carries out unified reagent again to whole sample crowd and handles, when changing the reagent, only need wholly mention inside processing box, discard old reagent, add new reagent can, the reagent change of all samples can be accomplished to once operation, has greatly promoted efficiency and has reduced human error. This architecture enables high t