CN-122016423-A - Sample separation device and sample separation method

CN122016423ACN 122016423 ACN122016423 ACN 122016423ACN-122016423-A

Abstract

The sample separation device comprises a fluid distribution component, at least one extraction component and a detection module, wherein the fluid distribution component is used for being communicated with a storage component, the storage component is used for storing a sample, the sample is provided with a plurality of sample layers, the extraction component is communicated with the fluid distribution component and is used for extracting one sample layer or sequentially extracting different sample layers, the detection module is arranged on an extraction pipeline between the extraction component and the fluid distribution component, a sample layering state detector in the detection module is used for emitting detection signals towards the sample layers flowing through the extraction pipeline and receiving the detection signals reflected by the sample layers, and the type of the sample layers flowing through the extraction pipeline is judged according to the difference value of the detection signals. The sample separation device has high automation degree, does not depend on manual visual layering, can accurately extract layering liquid of different layers, improves recovery purity and recovery rate of different sample layers, and can perform quantitative split charging.

Inventors

YANG YIJIE
CHEN HAIYUN
XIANG YUAN
LAI CHUAN
LUO JINGHUA
CHEN GUANFENG

Assignees

生命技术探索公司

Dates

Publication Date: 20260512
Application Date: 20260106

Claims (10)

1. A sample separation device is characterized by comprising a fluid distribution assembly, at least one extraction assembly and a detection module, Wherein the fluid dispensing assembly is configured to communicate with a storage assembly, the storage assembly configured to store a sample, the sample having a plurality of sample layers; the extraction assembly is communicated with the fluid distribution assembly and is used for extracting one sample layer in the storage assembly or sequentially extracting different sample layers in the storage assembly; The detection module is arranged on the extraction pipeline between the extraction component and the fluid distribution component, and comprises a sample layering state detector, wherein the sample layering state detector is used for emitting detection signals towards the sample layer flowing through the extraction pipeline and receiving detection signals reflected back by the sample layer so as to judge the type of the sample layer flowing through the extraction pipeline according to the difference value between the emitted detection signals and the reflected detection signals.
2. The sample separation apparatus of claim 1, wherein the detection module further comprises a flow detector for detecting flow through the sample layer of the extraction line.
3. The sample separation apparatus of claim 2, wherein the flow detector comprises an ultrasonic flow sensor that emits ultrasonic waves in a direction that is at an acute angle to the axis of the extraction line.
4. The sample separation apparatus of claim 1, wherein the extraction assembly comprises a power source and the extraction conduit, the power source for providing a driving force for fluid transfer, the power source in communication with the fluid distribution assembly through the extraction conduit or the power source in communication with a conduit of the fluid distribution assembly at an end remote from the extraction conduit.
5. The sample separation apparatus of claim 1, further comprising a storage assembly including a centrifuge and a storage container disposed on the centrifuge, the extraction assembly further configured to inject the sample into the storage container, the centrifuge configured to centrifugally layer the sample in the storage container to form the plurality of sample layers.
6. The sample separation apparatus of claim 5, wherein the sample dispensing system comprises a plurality of the extraction assemblies, one of the plurality of extraction assemblies being configured to inject the sample into the storage assembly, the other of the plurality of extraction assemblies being configured to extract the sample layer.
7. The sample separation apparatus of claim 1, wherein the sample stratification state detector comprises an infrared photosensor, and/or The sample separation device also comprises a user interaction module for displaying the split charging data and realizing user interaction.
8. A method of sample separation comprising: A plurality of sample layers are separated and recycled, In the process of separating and recycling each sample layer, a sample layering state detector is used for transmitting detection signals towards the sample layer flowing through an extraction pipeline and receiving the detection signals reflected by the sample layer so as to judge the type of the sample layer flowing through the extraction pipeline according to the difference value between the transmitted detection signals and the reflected detection signals.
9. The sample separation method of claim 8, wherein during separation of each of the sample layers, the method further comprises: The flow of the sample layer through the extraction line is detected by a flow detector.
10. The sample separation method of claim 9, wherein the flow detector comprises an ultrasonic flow sensor, and wherein the method of flow detection of the sample layer comprises: Transmitting ultrasonic waves by the ultrasonic flow sensor toward the sample layer flowing through the extraction line, the ultrasonic waves having a direction at an acute angle to the axis of the extraction line, and/or Before the step of separating and recovering the plurality of sample layers, the method further comprises: the sample is centrifuged such that the sample forms the plurality of sample layers.

Description

Sample separation device and sample separation method Technical Field The present application relates to the field of sample processing technologies, and in particular, to a sample separation device and a sample separation method. Background In order to accurately detect specific indexes and treat the diseases, the whole blood is generally divided into three layers by utilizing a centrifugal mode, the centrifugal separation is realized based on the density difference of blood components, and the three layers after the separation are respectively a light yellow and semitransparent platelet-rich plasma layer at the uppermost layer, an off-white platelet-rich plasma layer at the middle layer and a dark red cell layer at the lowermost layer. In various application scenarios, the blood products layered and positioned on different layers need to be transferred and extracted and used for subsequent biochemical application, such as clinical detection or component transfusion. The split charging of the separated blood is mainly manually and visually extracted at the present stage, the accuracy is low, components in other layers are easy to mix when one layer is extracted, the efficiency is low, and the loss of the blood components in each layer is easy to cause. Disclosure of Invention In view of the above, in order to solve at least one of the above problems, it is necessary to provide a sample separation device and a sample separation method. In a first aspect, the application provides a sample separation device comprising a fluid distribution assembly, at least one extraction assembly and a detection module, wherein the fluid distribution assembly is used for being communicated with a storage assembly, the storage assembly is used for storing a sample, the sample is provided with a plurality of sample layers, the extraction assembly is communicated with the fluid distribution assembly and is used for extracting one layer of the sample layers in the storage assembly or sequentially extracting different sample layers in the storage assembly, the detection module is arranged on an extraction pipeline between the extraction assembly and the fluid distribution assembly, the detection module comprises a sample layering state detector, and the sample layering state detector is used for transmitting detection signals towards the sample layers flowing through the extraction pipeline and receiving the detection signals reflected by the sample layers so as to judge the type of the sample layers flowing through the extraction pipeline according to the difference between the transmitted detection signals and the reflected detection signals. In some possible embodiments, the detection module further comprises a flow detector for detecting a flow of the sample layer through the extraction line. In some possible embodiments, the flow detector comprises an ultrasonic flow sensor that emits ultrasonic waves in a direction that is at an acute angle to the axis of the extraction line. In some possible embodiments, the extraction assembly includes a power source for providing a driving force for fluid transfer and the extraction line through which the power source communicates with the fluid distribution assembly or on a line of the fluid distribution assembly that is distal from the extraction line. In some possible embodiments, the sample separation device further comprises a storage assembly comprising a centrifuge and a storage container disposed on the centrifuge, the extraction assembly further configured to inject the sample into the storage container, the centrifuge configured to centrifugally stratify the sample in the storage container to form the plurality of sample layers. In some possible embodiments, the sample sub-packaging system comprises a plurality of the extraction assemblies, one of the plurality of extraction assemblies being configured to inject the sample into the storage assembly, and the other of the plurality of extraction assemblies being configured to extract the sample layer. In some possible embodiments, the sample layering state detector comprises an infrared photosensor, and/or the sample separation device further comprises a user interaction module for displaying the dispensing data and enabling user interaction. In a second aspect, an embodiment of the present application provides a sample separation method, including separating and recovering a plurality of sample layers, wherein during separation and recovery of each sample layer, a detection signal is emitted toward the sample layer flowing through an extraction pipeline by a sample layering state detector, and a detection signal reflected back by the sample layer is received, so as to determine a type of the sample layer flowing through the extraction pipeline according to a difference between the emitted detection signal and the reflected detection signal. In some possible embodiments, during separation of each of the sample layers, the method