CN-122016610-A - Counting cell

Abstract

The invention discloses a counting pool, which comprises a shell, wherein the shell comprises an upper cover and a bottom plate which are connected with each other, the upper cover and the bottom plate jointly form an inner cavity for accommodating a sample, the inner cavity comprises a first sub-inner cavity and a second sub-inner cavity which are communicated with each other, the first sub-inner cavity is smaller than the second sub-inner cavity in height, the upper cover and/or the bottom plate is provided with a window for an imaging mechanism to image the sample in the inner cavity, the upper cover is also provided with a sample inlet and an air outlet, the first sub-inner cavity is provided with a first communication opening leading to the sample inlet, the inner cavity is provided with a second communication opening leading to the air outlet, a transition structure is arranged between the first sub-inner cavity and the second sub-inner cavity, the transition structure comprises a first guide section and a demarcation section, the first guide section is arranged away from the second communication opening, and the transition structure is configured such that the sample in the first sub-inner cavity can be guided by the first guide section and can enter the second sub-inner cavity from the first guide section relative to the demarcation section more easily, and the problem of bubbles retained in the inner cavity can be improved.

Inventors

• YAN WEIWEI
• FENG XIANG
• Zhai Yashuang

Assignees

• 深圳迈瑞生物医疗电子股份有限公司

Dates

Publication Date

20260512

Application Date

20251110

Priority Date

20241111

Claims (20)

1. 1. The counting cell is characterized by comprising a shell, wherein the shell comprises an upper cover and a bottom plate which are connected with each other, the upper cover and the bottom plate jointly form an inner cavity for accommodating a sample, the inner cavity comprises a first sub-inner cavity and a second sub-inner cavity which are communicated with each other, the height of the first sub-inner cavity is smaller than that of the second sub-inner cavity, and the upper cover and/or the bottom plate is provided with a window which is used for an imaging mechanism to image the sample in the inner cavity; The upper cover is further provided with a sample inlet and an exhaust port, the sample inlet is communicated with the first sub-inner cavity and is used for allowing the sample to enter the first sub-inner cavity, the exhaust port is communicated with the inner cavity and is used for allowing gas to be discharged out of the inner cavity with the sample, the first sub-inner cavity is provided with a first communication port communicated with the sample inlet, and the inner cavity is provided with a second communication port communicated with the exhaust port; The transition structure comprises a first flow guide section and a demarcation section, the first flow guide section is arranged away from the second communication port relative to the demarcation Duan Yuan, the first flow guide section comprises a first flow guide surface, the first flow guide surface is respectively connected with a first cavity surface of the first sub-cavity and a second cavity surface of the second sub-cavity, the demarcation section comprises an interface, the interface is respectively connected with the first cavity surface and the second cavity surface, an included angle between the first flow guide surface and the first cavity surface is larger than an included angle between the interface and the first cavity surface, so that a sample in the first sub-cavity can be guided by the first flow guide surface and easily enter the second sub-cavity relative to the demarcation section from the first flow guide section.
2. 2. The counting chamber according to claim 1, wherein the first subchamber and the second communication port are disposed on a same side of the chamber, the transition structure further comprising a second flow guiding segment, the demarcation segment being disposed away from the second communication port relative to the second flow guiding segment.
3. 3. The counting chamber according to claim 2, wherein the second flow guiding section comprises a second flow guiding surface, the second flow guiding surface is respectively connected with the first cavity surface and the second cavity surface, and an included angle between the second flow guiding surface and the first cavity surface is larger than an included angle between the interface surface and the first cavity surface, so that a sample in the first sub-cavity can be guided by the second flow guiding surface to enter the second sub-cavity from the second flow guiding section more easily relative to the boundary section.
4. 4. A counter according to claim 3, wherein the second flow guiding surface is a sloping or curved surface and the dividing surface is a vertical surface; or the interface and the second diversion surface are inclined surfaces; or the interface and the second diversion surface are both curved surfaces.
5. 5. The counting cell of claim 2, wherein the second flow-directing segment includes a second flow-directing surface that is respectively coupled to the first and second cavity surfaces, an angle between the first flow-directing surface and the first cavity surface being greater than an angle between the second flow-directing surface and the first cavity surface, and an angle between the second flow-directing surface and the first cavity surface being greater than an angle between the interface surface and the first cavity surface, such that a sample within the first subchamber can be directed by the first flow-directing surface relative to the interface segment and the second flow-directing segment to more easily enter the second subchamber from the first flow-directing segment.
6. 6. The counting cell according to claim 2, wherein the axis of the second communication port intersects the side of the inner chamber where the first subchamber and the second communication port are provided.
7. 7. The counting cell according to claim 2, wherein the first flow guiding section extends to the furthest end of the transition structure from the second communication port and/or the second flow guiding section extends to the closest end of the transition structure from the second communication port.
8. 8. The counting assembly of claim 1, wherein the first subchamber and the second communication port are disposed on different sides of the chamber, the number of first flow-directing segments is two, and the demarcation segment is disposed between the two first flow-directing segments.
9. 9. The counting cell of claim 8, wherein the first subchamber is generally diagonally disposed with respect to the second communication port.
10. 10. The counting cell according to claim 1, wherein the first guide surface is an inclined surface or a curved surface, and the demarcation section is a vertical surface; or the first diversion surface and the interface are inclined surfaces; or the first diversion surface and the interface are both curved surfaces.
11. 11. The counting chamber according to claim 1, wherein the first subchamber is substantially fan-shaped in shape, the first communication port is provided in a center region of the fan-shaped, or the first subchamber is substantially triangular in shape, and the first communication port is provided in a vertex region of the triangle.
12. 12. The counting cell according to claim 1, wherein the second subchamber is provided with the second communication port to communicate the exhaust port with the second subchamber.
13. 13. The counting cell of claim 1, wherein an angle between the first flow-directing surface and the second cavity surface is greater than an angle between the interface surface and the second cavity surface.
14. 14. The counting cell according to claim 1, wherein the first cavity surface is a bottom surface of the first sub-cavity and is provided as a plane, and/or the second cavity surface is a bottom surface of the second sub-cavity and is provided as a plane.
15. 15. The counting cell of claim 14, wherein the top surface of the first subchamber is coplanar with the top surface of the second subchamber.
16. 16. The counting cell according to claim 1, wherein the first cavity surface is a top surface of the first sub-cavity and is provided as a plane, and/or the second cavity surface is a top surface of the second sub-cavity and is provided as a plane.
17. 17. The counting cell of claim 16, wherein the bottom surface of the first subchamber is coplanar with the bottom surface of the second subchamber.
18. 18. The counting cell is characterized by comprising a shell, wherein the shell comprises an upper cover and a bottom plate which are connected with each other, the upper cover and the bottom plate jointly form an inner cavity for accommodating a sample, the inner cavity comprises a first sub-inner cavity and a second sub-inner cavity which are communicated with each other, the height of the first sub-inner cavity is smaller than that of the second sub-inner cavity, and the upper cover and/or the bottom plate is provided with a window which is used for an imaging mechanism to image the sample in the inner cavity; The upper cover is further provided with a sample inlet and an exhaust port, the sample inlet is communicated with the first sub-inner cavity and is used for allowing the sample to enter the first sub-inner cavity, the exhaust port is communicated with the inner cavity and is used for allowing gas to be discharged out of the inner cavity with the sample, the first sub-inner cavity is provided with a first communication port communicated with the sample inlet, and the inner cavity is provided with a second communication port communicated with the exhaust port; The transition structure comprises a first diversion section and a demarcation section, the first diversion section is arranged away from the second communication opening relative to the demarcation Duan Yuan, and the arrangement mode of the first diversion section in the inner cavity is different from the arrangement mode of the demarcation section in the inner cavity, so that samples in the first sub-inner cavity can be guided by the first diversion section and enter the second sub-inner cavity from the first diversion section more easily relative to the demarcation section.
19. 19. The counting chamber of claim 18, wherein the first flow-directing segment is disposed in the cavity in a different configuration than the demarcation segment is disposed in the cavity, including a height variation of the cavity corresponding to the first flow-directing segment being less than a height variation of the cavity corresponding to the demarcation segment along the direction from the first subchamber to the second subchamber.
20. 20. The counting cell is characterized by comprising a shell, wherein the shell comprises an upper cover and a bottom plate which are connected with each other, the upper cover and the bottom plate jointly form an inner cavity for accommodating a sample, the inner cavity comprises a first sub-inner cavity and a second sub-inner cavity which are communicated with each other, the height of the first sub-inner cavity is smaller than that of the second sub-inner cavity, and the upper cover and/or the bottom plate is provided with a window which is used for an imaging mechanism to image the sample in the inner cavity; The upper cover is further provided with a sample inlet and an exhaust port, the sample inlet is communicated with the first sub-inner cavity and is used for allowing the sample to enter the first sub-inner cavity, the exhaust port is communicated with the inner cavity and is used for allowing gas to be discharged out of the inner cavity with the sample, the first sub-inner cavity is provided with a first communication port communicated with the sample inlet, and the inner cavity is provided with a second communication port communicated with the exhaust port; The transition structure comprises a first flow guide section and a demarcation section, the first flow guide section is arranged away from the second communication port relative to the demarcation Duan Yuan, the first flow guide section comprises a first flow guide surface, the first flow guide surface is respectively connected with a first cavity surface of the first sub-cavity and a second cavity surface of the second sub-cavity, the demarcation section comprises an interface, the interface is respectively connected with the first cavity surface and the second cavity surface, projections of the first cavity surface and the second cavity surface in the vertical direction are not overlapped, and an included angle between the first flow guide surface and the horizontal plane is smaller than an included angle between the interface and the horizontal plane, so that samples in the first sub-cavity can be guided by the first flow guide surface and easily enter the second sub-cavity relative to the demarcation section from the first flow guide section.

Description

Counting cell Technical Field The invention relates to the technical field of medical instruments, in particular to a counting cell. Background In the fields of life science, medicine and health, food, environmental protection and the like, image or counting analysis is often required to be carried out on liquid and components thereof, a flat counting tank is usually manufactured by transparent materials, a liquid sample is injected, and shooting counting is carried out after particles in the liquid sample are settled to the bottom of the tank in a certain mode (such as natural settlement and centrifugal settlement). The counting cell comprises a housing having an inner cavity for accommodating a sample, and a sample inlet and an exhaust port communicating with the inner cavity, the sample entering the inner cavity from the sample inlet and being exhausted from the inner cavity through the exhaust port, however, for a counting cell having inner cavities of different depths, the fluid follows different flow paths in the inner cavities of different depths, resulting in easy generation of bubbles in the inner cavities, affecting detection. Disclosure of Invention The present invention aims to solve at least one of the technical problems existing in the prior art. Therefore, the invention provides a counting tank which can reduce the retention of bubbles. According to a first embodiment of the present invention, a counting cell comprises a housing including an upper cover and a bottom plate connected to each other, the upper cover and the bottom plate together forming an inner cavity for accommodating a sample, the inner cavity including a first sub-inner cavity and a second sub-inner cavity communicated with each other, the first sub-inner cavity having a height smaller than that of the second sub-inner cavity (for ease of understanding, the first sub-inner cavity and the second sub-inner cavity are respectively named as a thin area and a thick area in some aspects of the present invention), the upper cover and/or the bottom plate having a window for an imaging mechanism to image the sample in the inner cavity; The upper cover is further provided with a sample inlet and an exhaust port, the sample inlet is communicated with the first subchamber and used for allowing the sample to enter the first subchamber, the exhaust port is communicated with the inner chamber and used for allowing gas to be discharged from the inner chamber with the sample, the first subchamber is provided with a first communication port leading to the sample inlet, and the inner chamber is provided with a second communication port leading to the exhaust port; The transition structure comprises a first flow guide section and a demarcation section, the first flow guide section is arranged away from the second communication port relative to the demarcation Duan Yuan, the first flow guide section comprises a first flow guide surface, the first flow guide surface is respectively connected with a first cavity surface of the first sub-cavity and a second cavity surface of the second sub-cavity, the demarcation section comprises an interface, the interface is respectively connected with the first cavity surface and the second cavity surface, an included angle between the first flow guide surface and the first cavity surface is larger than an included angle between the interface and the first cavity surface, so that a sample in the first sub-cavity can be guided by the first flow guide surface and easily enter the second sub-cavity relative to the demarcation section from the first flow guide section. The counting cell according to the embodiment of the invention has at least the following beneficial effects: Based on the above, in this embodiment, the sample inlet is communicated with the first sub-cavity, that is, the "thin-zone sample injection" scheme is adopted, because the height of the first sub-cavity is small, the flow of the liquid in the first sub-cavity is mainly affected by capillary action, unlike the situation that the liquid is usually driven by the liquid pressure, the liquid tends to fill the first sub-cavity first and then flow from the first sub-cavity to the second sub-cavity when flowing in the first sub-cavity, so that the embodiment can at least avoid the retention of bubbles in the first sub-cavity. On the other hand, in this embodiment, the first diversion section is further arranged through the transition structure between the first sub-inner cavity and the second sub-inner cavity, so that the sample breaks through randomly and becomes easier to break through from the specific first diversion section, and the first diversion section is arranged away from the second communication port relative to the demarcation section, so that the sample can firstly fill the area which is more away from the second communication port, and further the problem of easy bubble retention is solved. In other embodiments of the present invention