CN-122016613-A - Liquid drop dispensing monitoring system

CN122016613ACN 122016613 ACN122016613 ACN 122016613ACN-122016613-A

Abstract

The invention discloses a liquid drop distribution monitoring system, which abandons the traditional light shielding detection mode, adopts a charge detector induction detection method (EC: ELECTRIC CHARGE), and captures the image charge signal generated by charged liquid drops by arranging charge detectors consisting of a plurality of groups of induction electrodes at key positions of the flight path of the liquid drops, thereby accurately acquiring the charge state and the space position of the liquid drops. The invention effectively solves the problems of complex optical system, frequent calibration, easy interference of ambient light and the like in the prior art, and has the advantages of strong environmental adaptability, good stability, accurate detection, simple maintenance and the like.

Inventors

MIAO RUIFENG
LI JINDONG
LIU ZHENG

Assignees

深圳市特博赛科技有限公司

Dates

Publication Date: 20260512
Application Date: 20260205

Claims (10)

1. A liquid drop distribution monitoring system is characterized in that, A droplet generation module for dividing the continuous liquid stream into droplets; the charging module is used for charging the liquid drops; A plurality of charge detectors, each of the electrical measurement detectors including a plurality of sensing electrodes, the plurality of charge detectors being disposed on a flight path of the droplet; a plurality of said charge detectors are used to detect the charge state and spatial position of the droplet at the location.
2. The dispensing system of claim 1, wherein the plurality of charge detectors includes a first detector disposed in a central path of the droplet without the offset, the first detector being capable of generating a first charge pulse T1 if a charged droplet is detected; the device also comprises a processor, wherein the processor is used for recording the initial charging time T0 of the liquid drop, monitoring whether charge pulses exist in a set first time window, if so, determining that the disconnection position of the charged liquid drop and the liquid flow is stable, otherwise, prompting abnormality, wherein the first time window is a T0+ M+/-delta 1, delta 1<1/32 liquid drop period, and M is a fixed constant.
3. The dispensing system of claim 2, wherein the monitoring system further comprises at least one second detector disposed in the deflection path of the droplet, the second detector being capable of generating a second charge pulse T2 if a charged droplet is detected; the deflection electric field module is used for generating a deflection electric field, and the liquid drops deflect under the action of the deflection electric field; The processor is used for recording initial charging time T0 of the liquid drop, monitoring whether charge pulses exist in a set second time window, if so, determining that the deflection angle of the charged liquid drop is correct, otherwise, prompting abnormality, wherein the second time window is T0+ Q + -delta 2, delta 2<1.5 liquid drop periods, and Q is a fixed constant.
4. A distribution system according to claim 3, wherein the monitoring system further comprises a third detector for determining the charging phase and the charging voltage in a calibration scenario; The charging module and the electric field deflection module are used for charging the single liquid drop with a fixed first voltage, and the first voltage is smaller than a set value; The processor is used for controlling the charging module to gradually increase the charging phase and the charging voltage so as to deflect the liquid drops; The third detector detects that the charged liquid drop can generate a charge pulse, the amplitude of the charge pulse changes along with the increase of the charging voltage and the charging phase, and the ideal charging phase and the ideal voltage corresponding to the maximum amplitude of the charge pulse are determined.
5. The dispensing system of claim 4, wherein the third detector is further configured to determine whether a core module of the dispensing system is malfunctioning based on the presence or absence of a charge pulse detected by the third detector and whether the charge pulse is stable in an unsorted state.
6. The dispensing system of claim 5, further comprising a fourth detector disposed on a first offset path of the drop, the third detector disposed on a second offset path of the drop, the first offset path being on one side of the center path, the second offset path being on the other side of the center path, the first detector being at a greater vertical distance from the drop firing module than the third detector and the fourth detector are from the drop firing module, The processor determines whether the flow is bifurcated based on the first detector, the third detector, and the fourth detector.
7. The drop dispensing monitoring system of claim 6, wherein the processor controls the charging module to turn off the high voltage and charge the drops, records a pulse time T0 at which any one of the third detector and the fourth detector generates a pulse, and monitors whether the first detector, the third detector, and the fourth detector each generate a charge pulse within a set third time window, and if so, confirms that a bifurcation occurs.
8. The drop dispensing monitoring system of claim 7 wherein τ is between flights from the third detector to the first detector and the third time window is t0+3τ.
9. The drop dispensing monitoring system of claim 8, wherein the second and third and fourth detected detection signals determine whether a first anomaly is present based on the first detector, the first anomaly being a nozzle blockage.
10. The drop dispensing monitoring system of claim 9, wherein the number of second detectors is four, two of the second detectors are disposed on one side of the central path and two other of the second detectors are disposed on the other side of the central path, the second detectors being offset from the central path by an angle greater than the third and fourth detectors.

Description

Liquid drop dispensing monitoring system Technical Field The invention relates to the field of flow cell sorting, in particular to a liquid drop distribution monitoring system. Background In SIDE STREAM Monitor (bypass monitoring system), a light shielding detection mode is adopted for monitoring liquid flow and liquid drops, when a light source is shielded by the liquid flow and the liquid drops, shadows can be formed, when the shadows just fall on the photodiode PD, the photocurrent of the photodiode can be changed, and the liquid drops can be judged according to the change of the photocurrent. The method monitors each charged liquid drop, ensures that the deflection angle of each charged liquid drop is a set deflection angle, and is used for realizing the automatic control of the flow cell sorting module. The original process (piezoelectric driving voltage, droplet charging voltage and droplet charging phase) which needs manual adjustment is automated, stable liquid flow and breakpoint positions are maintained, whether a nozzle is blocked or not can be automatically monitored, and the sorting process is timely stopped. The optical system of the light shielding detection mode has high complexity, the light source, the liquid flow and the photoelectric detector must be aligned accurately, the installation and the debugging are complex, the light path offset can be caused by changing the nozzle, adjusting the liquid flow or micro vibration each time, and the frequent calibration is needed. Sensitive to ambient light, is susceptible to ambient light interference, and laboratory environment and light changes can affect detection signals. Disclosure of Invention The invention provides a liquid drop monitoring system with stronger environmental adaptability, higher signal-to-noise ratio and higher response speed. The application provides a liquid drop distribution monitoring system which comprises a liquid drop generation module, a charging module, a plurality of charge detectors, a plurality of sensing electrodes and a plurality of charge detectors, wherein the liquid drop generation module is used for dividing continuous liquid flow into liquid drops, the charging module is used for charging the liquid drops, each of the plurality of charge detectors comprises a plurality of sensing electrodes, the plurality of charge detectors are arranged on the flight path of the liquid drops, and the plurality of charge detectors are used for detecting the charge states and the space positions of the liquid drops on positions. In some embodiments, the plurality of charge detectors includes a first detector disposed on a central path of the droplet under an unbiased condition, the first detector being capable of generating a first charge pulse T1 if the first detector detects a charged droplet, and a processor configured to record an initial charging time T0 of the droplet, monitor whether there is a charge pulse within a set first time window, and if so, determine that a disconnected position of the charged droplet from the liquid stream is stable, and otherwise, prompt for an anomaly, wherein the first time window is t0+m±δ1, δ 1<1/32 droplet periods, and M is a fixed constant. In some embodiments, the monitoring system further comprises at least one second detector, the at least one second detector is arranged on a deflection path of the liquid drop, if charged liquid drops are detected, a second charge pulse T2 can be generated, a deflection electric field module is used for generating a deflection electric field, the liquid drops deflect under the action of the deflection electric field, the processor is used for recording initial charging time T0 of the liquid drops and monitoring whether charge pulses exist in a set second time window, if yes, the deflection angle of the charged liquid drops is determined to be correct, otherwise, abnormality is prompted, the second time window is T0 +Q+/-delta 2, delta 2<1.5 liquid drop periods, and Q is a fixed constant. In some embodiments, the monitoring system further comprises a third detector for determining a charging phase and a charging voltage in a calibration scenario, the charging module for charging individual droplets with a fixed first voltage, the first voltage being less than a set value, the processor for controlling the charging module to gradually increase the charging phase and the charging voltage to deflect the droplets, the third detector detecting that a charged droplet is capable of generating a charge pulse, the amplitude of the charge pulse varying with increasing charging voltage and charging phase, determining an ideal charging phase and an ideal voltage corresponding to the maximum amplitude of the charge pulse. In some embodiments, the third detector is further configured to determine, in an unsorted state, whether a core module of the distribution system is malfunctioning based on the presence or absence of a charge pulse detected by the third detec