CN-122016650-A - Biological material viscoelasticity measurement system based on stimulated Brillouin scattering

CN122016650ACN 122016650 ACN122016650 ACN 122016650ACN-122016650-A

Abstract

The application discloses a biological material viscoelasticity measurement system based on stimulated Brillouin scattering, which relates to the field of optical measurement and biomechanical characterization, and comprises a stimulated Brillouin scattering light source module, a laser source module and a control module, wherein the stimulated Brillouin scattering light source module is used for generating laser; the first optical fiber collimator is arranged on an output light path of the stimulated Brillouin scattering light source module; the beam splitter is arranged on the output light path of the first optical fiber collimator; the application is suitable for living biological samples.

Inventors

SHI JIULIN
HE XINGDAO
XIA NENGHAO
Ai Sizhu
WANG YIDI

Assignees

南昌航空大学

Dates

Publication Date: 20260512
Application Date: 20260316

Claims (8)

1. A biological material viscoelasticity measurement system based on stimulated Brillouin scattering is characterized in that, the stimulated Brillouin scattering-based biological material viscoelasticity measurement system comprises: The device comprises a stimulated Brillouin scattering light source module, a first optical fiber collimator, a beam splitter, an objective lens, a second optical fiber collimator, a third optical fiber and a viscoelasticity measurement module; The stimulated Brillouin scattering optical system comprises a stimulated Brillouin scattering light source module, a first optical fiber collimator, a beam splitter, an objective lens, a first optical fiber collimator, a second optical fiber collimator, a first optical fiber collimator, a beam splitter, a second optical fiber collimator, a lens and a lens, wherein the stimulated Brillouin scattering light source module is used for generating laser; The objective lens is also arranged on a backward scattering light path of the region to be detected of the biological material sample; the stimulated Brillouin backscattering light is transmitted to the objective lens through a backscattering light path, the objective lens is further used for focusing the stimulated Brillouin backscattering light onto the beam splitter, the beam splitter is further used for processing the stimulated Brillouin backscattering light output by the objective lens to generate reflected light, and the second optical fiber collimator is used for collimating the reflected light; The output end of the second optical fiber collimator is connected with the input end of the viscoelastic measurement module through a third optical fiber, and the viscoelastic measurement module is used for measuring the viscoelastic property of the region to be measured of the biological material sample according to the light transmitted by the third optical fiber.
2. The stimulated brillouin scattering-based biomaterial viscoelastic measurement system of claim 1, wherein the stimulated brillouin scattering-light source module includes: the laser comprises a laser, a first optical fiber, a modulator and a second optical fiber, wherein the output end of the laser is connected with the input end of the modulator through the first optical fiber, and the output end of the modulator is connected with the input end of the first optical fiber collimator through the second optical fiber.
3. The stimulated brillouin scattering-based biomaterial viscoelastic measurement system of claim 2, wherein the laser is a narrow linewidth continuous laser.
4. The stimulated brillouin scattering-based biomaterial viscoelastic measurement system as claimed in claim 3, wherein the modulator is an acousto-optic modulator.
5. The stimulated brillouin scattering-based biomaterial viscoelastic measurement system of claim 2, wherein the first and second optical fibers are both single-mode polarization maintaining fibers.
6. The stimulated brillouin scattering-based biomaterial viscoelastic measurement system of claim 2, wherein the viscoelastic measurement module comprises a brillouin spectrometer, an imaging device, and a data processing unit; the imaging device is arranged on an image plane of the Brillouin spectrometer and used for collecting and recording a spectrum image generated by the Brillouin spectrometer, and the data processing unit is used for measuring the viscoelasticity of a region to be measured of the biological material sample according to the spectrum image.
7. The stimulated brillouin scattering-based biomaterial viscoelastic measurement system of claim 6, wherein the brillouin spectrometer is a fabry-perot interferometer or a virtual imaging phased array spectrometer.
8. The stimulated brillouin scattering-based biomaterial viscoelastic measurement system of claim 6, wherein the imaging device is a camera.

Description

Biological material viscoelasticity measurement system based on stimulated Brillouin scattering Technical Field The application relates to the field of optical measurement and biomechanical characterization, in particular to a biological material viscoelasticity measurement system based on stimulated Brillouin scattering. Background The viscoelastic property of the biological material reflects the internal microstructure and mechanical response behavior of the biological material, and has important significance in disease diagnosis, tissue engineering, biological material design and basic life science research. Existing biomechanical measurement means, such as atomic force microscopy, micro-rheological testing and macro-mechanical stretching and compression experiments, generally need to be in contact with a sample, or high spatial resolution and in-situ measurement are difficult to achieve, so that the application of the method in living samples and micro-scale structures is limited. Optical techniques, such as brillouin scattering, can reflect its mechanical properties by detecting spontaneous thermal acoustic waves inside the material, but its signal is usually extremely weak, and it is difficult to obtain high quality spectral information especially in weakly scattering biological samples. Stimulated brillouin scattering can obviously enhance a brillouin scattering signal by inducing sound waves through strong laser. However, the existing biological material viscoelastic measurement system based on stimulated brillouin scattering can only perform elastic imaging on thinner samples such as cells, and limits the application of the system in living biological sample research. Thus, there is a need for a biological material viscoelastic measurement system suitable for use with biological samples of living subjects. Disclosure of Invention The application aims to provide a biological material viscoelasticity measurement system based on stimulated Brillouin scattering, which is suitable for a living biological sample. The application provides a biological material viscoelasticity measurement system based on stimulated Brillouin scattering, which comprises a stimulated Brillouin scattering light source module, a first optical fiber collimator, a beam splitter, an objective lens, a second optical fiber collimator, a third optical fiber and a viscoelasticity measurement module. The stimulated Brillouin scattering light source module is used for generating laser, the first optical fiber collimator is arranged on an output light path of the stimulated Brillouin scattering light source module, and the beam splitter is arranged on the output light path of the first optical fiber collimator. The optical path output by the first optical fiber collimator is processed by the beam splitter to generate transmission light, and the objective lens is used for focusing the transmission light to a region to be detected of a biological material sample to generate stimulated Brillouin back scattering light. The objective lens is also arranged on a backward scattering light path of the region to be detected of the biological material sample; the optical fiber collimator comprises an objective lens, a beam splitter, a second optical fiber collimator, a stimulated Brillouin backscattering light source, a beam splitter, a second optical fiber collimator and a second optical fiber collimator, wherein the beam splitter is further arranged on an output light path of the objective lens, the second optical fiber collimator is arranged on a reflection light path of the beam splitter, stimulated Brillouin backscattering light is transmitted to the objective lens through a backscattering light path, the objective lens is further used for focusing the stimulated Brillouin backscattering light onto the beam splitter, the beam splitter is further used for processing the stimulated Brillouin backscattering light output by the objective lens to generate reflection light, and the second optical fiber collimator is used for collimating the reflection light. The output end of the second optical fiber collimator is connected with the input end of the viscoelastic measurement module through a third optical fiber, and the viscoelastic measurement module is used for measuring the viscoelastic property of the region to be measured of the biological material sample according to the light transmitted by the third optical fiber. According to the specific embodiment of the application, the biological material viscoelastic measurement system based on stimulated Brillouin scattering has the technical effects that the biological material viscoelastic measurement system based on stimulated Brillouin scattering is provided, a first optical fiber collimator is arranged on an output light path of a stimulated Brillouin scattering light source module, a beam splitter is arranged on an output light path of the first optical fiber collimator, an objective lens is arr