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CN-122016657-A - Light sheet microscope sample cell

CN122016657ACN 122016657 ACN122016657 ACN 122016657ACN-122016657-A

Abstract

The invention relates to the technical field of sample cells for light sheet microscopes and discloses a light sheet microscope sample cell which comprises a box body, at least one side surface of the box body is provided with a first mounting hole, the bottom of the box body is provided with a second mounting hole, at least one side surface connector is provided with a curved surface side window, each side surface connector is provided with a bottom connector, a light transmitting piece is arranged on each bottom connector, the side surface connectors are detachably arranged in the first mounting holes, the curved surface side window is fixed on the side surface connectors to replace the traditional bonding process, the curved surface side window is convenient to replace, the high-precision concentric mounting of the curved surface side window is ensured, the bottom connectors with various height specifications can be arranged, the working distance requirements of different detection objective lenses are different, the bottom connectors with corresponding height specifications can be conveniently replaced to adapt to the working distance of the detection objective lenses in observation, and the whole sample cell is not required to be replaced.

Inventors

  • ZHAO TENG
  • LIU XIAOWEI
  • HUANG GUOFENG
  • ZHAO LUWEI

Assignees

  • 光原科技(深圳)有限公司

Dates

Publication Date
20260512
Application Date
20260206

Claims (10)

  1. 1. A light sheet microscope cuvette, comprising: the device comprises a box body (1) for placing a sample, wherein at least one side surface of the box body (1) is provided with a first mounting hole (2), and the bottom of the box body (1) is provided with a second mounting hole (3); At least one side connector, each side connector is detachably arranged in each first mounting hole (2) in a one-to-one correspondence manner, and each side connector is provided with a curved side window (5); The bottom connector (8) is detachably arranged in the second mounting hole (3), and a light transmission piece is arranged on the bottom connector (8).
  2. 2. The light sheet microscope sample cell according to claim 1, characterized in that an axial stop for axially positioning the bottom connector (8) and a circumferential stop for preventing rotation of the bottom connector (8) in the second mounting hole (3) are provided in the second mounting hole (3) and/or in the bottom connector (8).
  3. 3. A light sheet microscope cuvette according to claim 2, characterized in that the axial stop comprises a first boss (4) and a second boss (80); The first boss (4) is fixedly arranged on the inner side of the second mounting hole (3), the second boss (80) is arranged on the outer side of the bottom connector (8), and the second boss (80) is axially abutted with the first boss (4); The circumferential rotation stopping piece comprises a first positioning hole (31) and a first positioning protrusion (801); the first positioning hole (31) is formed in the periphery of the second mounting hole (3), the first positioning protrusion (801) is arranged on the side wall of the second boss (80), and the first positioning protrusion (801) is in matched insertion connection with the first positioning hole (31).
  4. 4. A light sheet microscope cuvette according to claim 1, characterized in that the light transmissive element comprises a glass bottom window (813), the bottom connector (8) comprising a first connector (81); the first connector (81) comprises a first main body (810), a third mounting hole (811) is formed in the middle of the first main body (810), a third boss (812) is fixedly arranged in the third mounting hole (811), and the glass bottom window (813) is mounted in the third mounting hole (811).
  5. 5. The light sheet microscope sample cell according to claim 4, wherein second positioning holes (814) are formed at two circumferential ends of the third mounting hole (811), a positioning ring (815) and a third fixing collar (816) are further mounted in the first main body (810), and second positioning protrusions (817) matched with the second positioning holes (814) are formed at two ends of the positioning ring (815); The third fixing retainer ring (816) is connected with the third mounting hole (811) to press the positioning ring (815) and the glass bottom window (813).
  6. 6. A light sheet microscope cuvette according to claim 1, characterized in that the light transmissive element comprises a light transmissive film (823) and the bottom connector (8) comprises a second connector (82); the second connector (82) comprises a second main body (820), a fourth through hole (821) is formed in the middle of the second main body (820), a fourth boss (822) is fixedly arranged in the fourth through hole (821), and the light-transmitting film (823) covers the fourth boss (822) and the outer wall of the second main body (820).
  7. 7. The light sheet microscope cuvette as defined in claim 6, wherein the light transmissive film (823) is made of PDMS.
  8. 8. A light sheet microscope sample cell according to claim 1, characterised in that each side connector comprises a first fixing collar (6), the first fixing collar (6) being connected with each first mounting hole (2) to press each curved side window (5); the light sheet microscope sample cell further comprises a second fixed check ring (7), and the second fixed check ring (7) is connected with the second mounting hole (3) to press the bottom connector (8).
  9. 9. A light sheet microscope sample cell according to any one of claims 1 to 8, characterised in that the exterior of each side wall of the housing (1) is fitted with a heating element (9) and a mounting wall (11); The outer side wall of each side wall of the box body (1) is provided with a first magnetic attraction hole (10), and a first magnet is arranged in each first magnetic attraction hole (10); the mounting walls (11) are provided with second magnets magnetically attracted with the first magnets at positions corresponding to the first magnetic attraction holes (10), and the mounting walls (11) are attached to the outer side walls of the box body (1) to clamp the heating element (9); the mounting wall (11) and the heating element (9) are provided with avoidance holes (12) corresponding to the first mounting holes (2).
  10. 10. A light sheet microscope sample cell according to any one of claims 1-8 further comprising a base connector (13); the base connector (13) is magnetically connected with the bottom of the box body (1).

Description

Light sheet microscope sample cell Technical Field The invention relates to the technical field of sample cells for light sheet microscopes, in particular to a sample cell for a light sheet microscope. Background In order to realize high-resolution three-dimensional imaging of a sample in an inverted lens microscope system, the core structural characteristics of the inverted lens microscope system are that an illumination light path and a detection light path are separated in a space orthogonal mode, a plurality of illumination objective lenses are horizontally irradiated from the side of the sample, the detection objective lenses are vertically inverted to be positioned below the sample for observation, a sample cell arranged in the center is not only required to provide a light transmission opening for the illumination objective lenses from the side, but also required to provide an observation opening for the inverted detection objective lenses from the bottom. In the sample cell in the prior art, curved side windows are adhered in the light transmission openings at the sides, and bottom windows are adhered to the observation openings at the bottom, so that the bottom windows can be matched with the working distance of the corresponding detection objective lens, and the objective lens can be ensured to be accurately focused on the sample. However, the curved side window with a focusing function, which is fixed by the traditional bonding process, is difficult to realize and maintain high-precision concentricity, so that aberration is generated in an illumination light path, and the final imaging quality of the system is directly reduced; when the detection objective lens with different numerical apertures is required to be replaced according to the observation requirement, the working distance of the detection objective lens can be changed, the bottom window and the sample cell main body are adhered into a whole, the height of the detection objective lens cannot be independently adjusted or the bottom window of different types can not be replaced, for example, the detection objective lens is switched from a low-power mirror to a high-power mirror, the working distance is shortened, the height of the bottom window is required to be adapted to a new distance, at the moment, the original adhered bottom window cannot be adjusted, a user can only replace the whole sample cell, and the mode of 'one-time and full-change' is complex in operation, and greatly increases the use cost and complexity due to frequent replacement of integral parts. Disclosure of Invention The invention aims to solve the technical problems that in the design of a sample cell of a microscope system of the existing inverted lens, a curved side window and a bottom window are fixed in an adhesive mode, the curved side window is difficult to realize and maintain high-precision concentricity, so that aberration is generated in an illumination light path, the final imaging quality of the system is directly reduced, and the bottom window cannot be independently disassembled and replaced to adapt to different optical requirements, so that the mode of 'one-change-over-all-change' is complex in operation, and the production cost is greatly increased due to frequent replacement of integral parts. In order to solve the technical problems, the present invention provides a light sheet microscope sample cell, comprising: the box body is used for placing a sample, at least one side surface of the box body is provided with a first mounting hole, and the bottom of the box body is provided with a second mounting hole; At least one side connector, each side connector is detachably arranged in each first mounting hole in a one-to-one correspondence manner, and each side connector is provided with a curved side window; the bottom connector is detachably arranged in the second mounting hole, and a light-transmitting piece is arranged on the bottom connector. Preferably, an axial stop member is provided in the second mounting hole and/or in the bottom connector, the axial stop member being used for axially positioning the bottom connector, and a circumferential stop member being used for preventing the bottom connector from rotating in the second mounting hole. Preferably, the axial stop comprises a first boss and a second boss; the first boss is fixedly arranged at the inner side of the second mounting hole, the second boss is arranged at the outer side of the bottom connector, and the second boss is axially abutted with the first boss; The circumferential rotation stopping piece comprises a first positioning hole and a first positioning protrusion; The first locating hole is arranged on the periphery side of the second mounting hole, the first locating protrusion is arranged on the side wall of the second boss, and the first locating protrusion is matched and inserted with the first locating hole. Preferably, the light transmissive member comprises a glass bott