CN-122016731-A - Dual-function SPR biosensor based on anisotropic material and method thereof

CN122016731ACN 122016731 ACN122016731 ACN 122016731ACN-122016731-A

Abstract

The invention discloses a dual-function SPR biosensor based on an anisotropic material, which comprises a Kretschmann prism coupling structure, wherein the Kretschmann prism coupling structure comprises a prism layer, a coupling medium layer, an anisotropic functional layer, a metal layer and a sensing medium layer, the prism layer comprises an incidence surface, a reflection surface and a detection surface, the incidence surface receives irradiation of detection light, the reflection surface outputs the detection light reflected by the detection surface, the detection surface of the prism layer is sequentially covered with the coupling medium layer, the anisotropic functional layer, the metal layer and the sensing medium layer, the anisotropic functional layer is a two-dimensional material layer with in-plane optical anisotropy, the anisotropic functional layer comprises a first crystal orientation and a second crystal orientation, the performance optimization mode of the SPR biosensor under surface plasmon resonance is switched by changing the crystal orientation of the anisotropic functional layer in the plane, and when the anisotropic functional layer is in the first crystal orientation or the second crystal orientation, the SPR biosensor is in an optimized high-sensitivity mode or an optimized high-quality factor mode.

Inventors

Wei Chenwai
CAO YUE
SUN HAO

Assignees

无锡学院

Dates

Publication Date: 20260512
Application Date: 20260409

Claims (9)

1. The dual-function SPR biosensor based on the anisotropic material is characterized by comprising a Kretschmann prism coupling structure, wherein the Kretschmann prism coupling structure comprises a prism layer (1), a coupling medium layer (2), an anisotropic functional layer (3), a metal layer (4) and a sensing medium layer (5), the prism layer (1) comprises an incidence surface, a reflection surface and a detection surface, the incidence surface receives irradiation of detection light, the reflection surface outputs the detection light reflected by the detection surface, the detection surface of the prism layer (1) is sequentially covered with the coupling medium layer (2), the anisotropic functional layer (3), the metal layer (4) and the sensing medium layer (5), the anisotropic functional layer (3) is a two-dimensional material layer with in-plane optical anisotropy, and the anisotropic functional layer (3) comprises a first crystal orientation and a second crystal orientation; The performance optimization mode of the SPR biosensor under the surface plasmon resonance is switched by changing the crystal orientation of the anisotropic functional layer (3) in the plane, the SPR biosensor is in an optimized high-sensitivity mode when the anisotropic functional layer (3) is in a first crystal orientation, and the SPR biosensor is in an optimized high-quality factor mode when the anisotropic functional layer (3) is in a second crystal orientation.
2. The dual function SPR biosensor based on anisotropic material according to claim 1, wherein the first crystal orientation of the anisotropic functional layer (3) is the X crystal axis direction of the anisotropic functional layer, and the second crystal orientation of the anisotropic functional layer (3) is the Y crystal axis direction of the anisotropic functional layer.
3. The dual-function SPR biosensor based on anisotropic material according to claim 1, wherein the anisotropic functional layer (3) is made of alpha-phase molybdenum trioxide, and the thickness of the anisotropic functional layer is 100-500 nm.
4. The dual-function SPR biosensor based on anisotropic material according to claim 1, wherein the coupling medium layer (2) is made of low refractive index optical medium, and is made of fluoropolymer CYTOP, and the thickness of the coupling medium layer is 80-120 nm.
5. The dual-function SPR biosensor based on anisotropic material according to claim 1, wherein the metal layer (4) comprises one of gold and silver, and the thickness of the metal layer is 50nm-100nm.
6. The dual function SPR biosensor based on anisotropic material according to claim 5, wherein the metal layer (4) is a stepped metal layer.
7. The dual-function SPR biosensor based on anisotropic materials according to claim 1, wherein the sensing medium layer (5) is provided with an object to be detected, and the object to be detected is subjected to two detection modes of wavelength detection and angle detection.
8. The dual-function SPR biosensor based on anisotropic materials according to claim 7, wherein the SPR biosensor can dynamically switch between an optimized high-sensitivity mode and an optimized high-quality factor mode when detecting the wavelength of the target to be detected, and wherein the SPR biosensor can dynamically switch between the optimized high-sensitivity mode and the optimized high-quality factor mode when detecting the angle of the target to be detected.
9. The method for operating the dual-function SPR biosensor based on the anisotropic material according to the claim 1-8, is characterized in that the performance optimization mode of the SPR biosensor under the surface plasmon resonance is switched according to the detection requirement of wavelength detection or angle detection, comprising the following steps: step 1, judging that the current detection requirement is to detect the tiny change of the refractive index or detect a high-precision resonance signal; Step 2, when the detection requirement is that the refractive index is detected to be slightly changed, adjusting the anisotropic functional layer (3) to a first crystal orientation, wherein the SPR biosensor is in an optimized high-sensitivity mode; and 3, when the detection requirement is that the high-precision resonance signal is detected, adjusting the anisotropic functional layer (3) to a second crystal orientation, and optimizing the SPR biosensor in a high-quality factor mode.

Description

Dual-function SPR biosensor based on anisotropic material and method thereof Technical Field The invention relates to the technical field of optical sensing and biological detection, in particular to a dual-function SPR biosensor based on an anisotropic material and a method thereof. Background The surface plasma resonance biosensor has the advantages of no need of marking, real-time monitoring and the like, and is widely applied to the fields of biomolecule interaction analysis, clinical diagnosis and the like. The performance core indicators include sensitivity (S) and figure of merit (FOM). Conventional SPR sensors are structurally fixed, and S and FOM are usually constrained to each other, and are difficult to dynamically optimize according to different detection requirements, for example, high sensitivity is required to detect low concentration analytes, or high FOM is required to achieve high-precision measurement dynamic optimization. Users often need to replace sensors with different designs or complex external tuning devices to realize detection of different emphasis points, and the detection method is complex in operation and high in cost. In recent years, there have been attempts to introduce anisotropic materials into SPR structures to enhance performance, but most focus has been on achieving single index enhancement using their fixed directional characteristics. How to utilize the anisotropic property of material, realize that the performance mode can initiatively, convenient switch on same sensor, make it can intelligent adaptation different detection scenes, keep the compatibility to wavelength and angle dual detection mode simultaneously, be the technical problem who needs to solve in the field. Disclosure of Invention The invention aims to: in order to overcome the defects in the prior art, the invention provides the dual-function SPR biosensor based on the anisotropic material and the method thereof, and the high-sensitivity model and the high-quality factor model are optimized by dynamically switching under the condition of compatible wavelength and angle dual-detection mode by introducing the anisotropic functional layer into the SPR biosensor. The technical scheme is that the dual-function SPR biosensor based on the anisotropic material comprises a Kretschmann prism coupling structure, wherein the Kretschmann prism coupling structure comprises a prism layer, a coupling medium layer, an anisotropic function layer, a metal layer and a sensing medium layer, the prism layer comprises an incidence surface, a reflection surface and a detection surface, the incidence surface receives irradiation of detection light, the reflection surface outputs the detection light reflected by the detection surface, the detection surface of the prism layer is sequentially covered with the coupling medium layer, the anisotropic function layer, the metal layer and the sensing medium layer, the anisotropic function layer is a two-dimensional material layer with in-plane optical anisotropy, and the anisotropic function layer comprises a first crystal orientation and a second crystal orientation. The performance optimization mode of the SPR biosensor under the surface plasmon resonance is switched by changing the in-plane crystal orientation of the anisotropic functional layer, the SPR biosensor is in an optimized high-sensitivity mode when the anisotropic functional layer is in a first crystal orientation, and the SPR biosensor is in an optimized high-quality factor mode when the anisotropic functional layer is in a second crystal orientation. Further, the first crystal direction of the anisotropic functional layer is the X crystal axis direction of the anisotropic functional layer, and the second crystal direction of the anisotropic functional layer is the Y crystal axis direction of the anisotropic functional layer. Further, the material of the anisotropic functional layer is alpha-phase molybdenum trioxide, and the thickness of the anisotropic functional layer is 100nm-500nm. Furthermore, the coupling medium layer is made of low refractive index optical medium, fluoropolymer CYTOP is selected, and the thickness of the coupling medium layer is 80-120 nm. Further, the metal layer comprises one of gold and silver, and the thickness of the metal layer is 50nm-100nm. Further, the metal layer is a stepped metal layer. Furthermore, the sensing medium layer is provided with a target to be detected, and two detection modes of wavelength detection and angle detection are carried out on the target to be detected. Furthermore, when the SPR biosensor detects the wavelength of the target to be detected, the optimal high-sensitivity mode and the optimal high-quality factor mode can be dynamically switched, and when the SPR biosensor detects the angle of the target to be detected, the optimal high-sensitivity mode and the optimal high-quality factor mode can be dynamically switched. Further, the working method of the dual-fun