CN-122016775-A - Bionic colorimetric sensing platform for indicating food freshness

CN122016775ACN 122016775 ACN122016775 ACN 122016775ACN-122016775-A

Abstract

The application provides a bionic colorimetric sensing platform for indicating food freshness, which comprises a substrate and N gel columns arranged on the substrate, wherein each gel column is divided into an adsorption layer and a sensing layer, the adsorption layer is used for adsorbing target detection objects so as to realize enrichment and diffusion of the target detection objects, one surface of the sensing layer is directly combined with the substrate, the other surface of the sensing layer is combined with one surface of the adsorption layer, which is close to the substrate, the sensing layer is used for receiving the target detection objects diffused by the adsorption layer, the sensing layer is loaded with a dyeing substance, the dyeing substance and the target detection objects undergo colorimetric reaction, and the existence and the content of the target detection objects are reflected through the change of optical signals of the dyeing substance. The application realizes high-sensitivity detection of characteristic spoilage gas and food-borne pathogens, can effectively evaluate early spoilage of foods, and is simple and convenient to apply.

Inventors

CHEN SHUAI
ZHANG YU
ZHANG XIAO
YU YONGLIANG

Assignees

东北大学
东北大学佛山研究生创新学院

Dates

Publication Date: 20260512
Application Date: 20260226

Claims (9)

1. The bionic colorimetric sensing platform for indicating the freshness of the food comprises a substrate and is characterized by further comprising N gel columns arranged on the substrate, wherein each gel column is divided into an adsorption layer and a sensing layer; the adsorption layer is used for adsorbing the target detection object so as to realize enrichment and diffusion of the target detection object; The sensing layer is used for receiving target detection objects diffused by the adsorption layer, the sensing layer is loaded with dyeing substances, the dyeing substances react with the target detection objects in a colorimetric way, and the existence and the content of the target detection objects are reflected through the change of optical signals of the dyeing substances.
2. The biomimetic colorimetric sensing platform for indicating food freshness according to claim 1, wherein the material of the adsorption layer is a stimulus-responsive hydrogel, and the material of the sensing layer is a synthetic polymer-based double-network hydrogel.
3. The biomimetic colorimetric sensing platform for indicating food freshness according to claim 2, wherein the stimulus-responsive hydrogel is an agar hydrogel and the polymer-based dual-network hydrogel is a polyvinyl alcohol and polyacrylamide dual-network hydrogel.
4. The bionic colorimetric sensing platform for indicating food freshness according to claim 2, wherein the adsorption layer comprises a nano-porous material for capturing and selectively enriching a target detection object in a hydration microenvironment formed by a stimulus-responsive hydrogel.
5. The biomimetic colorimetric sensing platform for indicating food freshness according to claim 4, wherein the nano-porous material is a ZIF-8 nano-material, and the ZIF-8 nano-material is etched into a hollow structure.
6. The biomimetic colorimetric sensing platform for indicating food freshness according to claim 5, wherein the ratio of the feeding mass of the ZIF-8 nanomaterial to the feeding mass of the agar hydrogel is 1:5.
7. The biomimetic colorimetric sensing platform for indicating food freshness according to claim 3, wherein the ratio of the average thickness of the agar hydrogel to the average thickness of the polyvinyl alcohol and polyacrylamide double network hydrogel is 1:4.
8. The biomimetic colorimetric sensing platform for indicating the freshness of a food product according to claim 1, wherein the staining substance is a chemically responsive dye.
9. The biomimetic colorimetric sensing platform for indicating food freshness according to claim 8, wherein the chemically responsive dye comprises one or any of 2, 4-dinitrophenylhydrazine and sulfuric acid, zinc nitrate and bromophenol blue, p-Luo Shalin, tetraphenylcobalt porphyrin (II), tetraphenylzinc porphyrin (II), tetrachloro-p-benzoquinone, methyl orange, methyl red and bromothymol blue, phenol red.

Description

Bionic colorimetric sensing platform for indicating food freshness Technical Field The application belongs to the technical field of food detection, and particularly relates to a bionic colorimetric sensing platform for indicating food freshness. Background Currently, many techniques have been developed for the detection of the quality of refrigerated food. The traditional detection method has the problems of complex operation, long time consumption, destructiveness to samples and the like although the accuracy is high, and the fluorescent method, the enzyme detection based on a biosensor, the gas-sensitive electrochemical sensor and other novel methods realize the detection of the metabolic markers such as volatile nitrogen compounds, sulfides, organic acids and the like generated in the food spoilage process. The existing refrigerated food quality detection method mainly has the defects that the detection result of the traditional chemical index (such as volatile basic nitrogen) is often delayed from the actual sensory deterioration of the food, early spoilage cannot be effectively estimated, and many detection methods (such as high performance liquid chromatography) have complex steps, consume long time and damage samples. Emerging rapid and real-time detection techniques (e.g., mass spectrometry, smart tags) face challenges such as high cost, complex operation, insufficient portability, and potential for reduced stability and reproducibility in actual complex samples. Disclosure of Invention In order to solve the technical problems, the application provides a bionic colorimetric sensing platform for indicating food freshness. In a first aspect, the application provides a bionic colorimetric sensing platform for indicating food freshness, which comprises a substrate, and further comprises N gel columns arranged on the substrate, wherein each gel column is divided into an adsorption layer and a sensing layer; the adsorption layer is used for adsorbing the target detection object so as to realize enrichment and diffusion of the target detection object; The sensing layer is used for receiving target detection objects diffused by the adsorption layer, the sensing layer is loaded with dyeing substances, the dyeing substances react with the target detection objects in a colorimetric way, and the existence and the content of the target detection objects are reflected through the change of optical signals of the dyeing substances. Optionally, the material of the adsorption layer is stimulus-responsive hydrogel, and the material of the sensing layer is synthetic polymer-based double-network hydrogel. Optionally, the stimulus-responsive hydrogel is an agar hydrogel and the polymer-based double-network hydrogel is a polyvinyl alcohol and polyacrylamide double-network hydrogel. Optionally, the adsorption layer contains a nano porous material, and the nano porous material is used for capturing and selectively enriching target detection objects in a hydration microenvironment formed by the stimulus-responsive hydrogel. Optionally, the nano porous material is a ZIF-8 nano material, and the ZIF-8 nano material is etched into a hollow structure. Optionally, the charging mass ratio of the ZIF-8 nanomaterial to the agar hydrogel is 1:5. Alternatively, the ratio of the average thickness of the agar hydrogel to the average thickness of the polyvinyl alcohol and polyacrylamide dual-network hydrogel is 1:4. Optionally, the staining substance is a chemically responsive dye. Optionally, the chemical responsive dye comprises one or any of 2, 4-dinitrophenylhydrazine and sulfuric acid, zinc nitrate and bromophenol blue, p-Luo Shalin, tetraphenyl cobalt porphyrin (II), tetraphenyl zinc porphyrin (II), tetrachloro-p-benzoquinone, methyl orange, methyl red and bromothymol blue and phenol red. Advantageous effects 1. The application realizes high-sensitivity detection of characteristic spoilage gas and food-borne pathogens; 2. The detection limit of trimethylamine (TRIMETHYLAMINE) is as low as 0.02 ppm, and the application can rapidly identify and detect volatile spoilage markers such as 2-nonone (2-nonanone), glutaraldehyde and trimethylamine; 3. The application can effectively detect various common food-borne pathogens, wherein the common food-borne pathogens comprise staphylococcus aureus (S. Aureus), enterohemorrhagic escherichia coli (EHEC), listeria monocytogenes (L. Mono) and Salmonella (Salmonella). Drawings FIG. 1 is a schematic structural diagram of a bionic colorimetric sensing platform for indicating food freshness according to an embodiment of the application; FIG. 2 is a flow chart of a preparation process of the hollow ZIT-8 nanomaterial according to an embodiment of the present application; Wherein 1 is a bionic colorimetric sensing platform, 2 is an adsorption layer, 3 is a sensing layer, 4 is a nano porous material, 5 is a chemical responsive dye, 6 is a dyeing product, 7 is a substrate, and 8 is a gel column. Detail