CN-122016777-A - Rapid detection system for atrazine screening in food samples

CN122016777ACN 122016777 ACN122016777 ACN 122016777ACN-122016777-A

Abstract

The invention relates to the technical field of material detection, in particular to a rapid detection system for atrazine screening in food samples. The system comprises a detection zone image and a water absorption pad area image, wherein the background gray value of the background area image of the test paper is removed from the gray value in the detection zone area image and the water absorption pad area image at each sampling time, the color development intensity of the detection zone and the color development intensity of the water absorption pad are sequentially obtained, the detection zone area is divided into an upstream area and a downstream area, the color development intensity difference of the detection zone area and the downstream area is obtained, the blocking growth degree is obtained according to the change trend distribution of the color development intensity difference along with the detection zone excessive amount, and the blocking detection is sequentially carried out according to the detection zone excessive amount, the detection zone color development intensity and the blocking growth degree at the final time. The invention can effectively distinguish false positive caused by physical blockage from true positive caused by chemical inhibition, and improves the detection accuracy.

Inventors

QIN LEI
CHEN XIAOTING
Su Yingshi
ZHU WEI

Assignees

齐齐哈尔医学院

Dates

Publication Date: 20260512
Application Date: 20260325

Claims (10)

1. A rapid detection system for atrazine screening in a food sample, the system comprising: the optical data acquisition module is used for continuously acquiring a plurality of test paper images at the moment of liquid contact, wherein the test paper images comprise a detection zone area image, a test paper background area image and a water absorption pad area image; the flow data quantization module is used for respectively removing the background gray value of the test paper background area image from the gray value in the detection area image and the water absorption pad area image under each sampling time to sequentially obtain the color development intensity of the detection area and the color development intensity of the water absorption pad; The detection zone material change analysis module is used for dividing the detection zone into an upstream zone and a downstream zone, and obtaining the color development intensity difference between the color development intensity of the upstream zone and the color development intensity of the downstream zone; And the color development detection module is used for carrying out blocking detection according to the detection band excess, the detection band color development intensity and the blocking growth degree at the final moment in sequence.
2. The rapid detection system for atrazine screening in a food sample according to claim 1, wherein the method for obtaining the background gray value comprises: And for each sampling time, taking the average gray value in the background area image of the test paper as the background gray value at the corresponding sampling time.
3. The rapid detection system for atrazine screening in a food sample according to claim 1, wherein the method for obtaining the color development intensity of the test strip comprises: and for each sampling moment, obtaining a first difference value of subtracting the average gray value in the detection zone region image from the background gray value, if the first difference value is larger than 0, taking the first difference value as the color development intensity of the detection zone, and if the first difference value is smaller than or equal to 0, setting the color development intensity of the detection zone at the corresponding sampling moment to be 0.
4. The rapid detection system for atrazine screening in a food sample according to claim 1, wherein the method for obtaining the development intensity of the absorbent pad comprises: And for each sampling moment, obtaining a second difference value of the background gray value minus the average gray value in the image of the water absorption pad area, if the second difference value is larger than 0, taking the first difference value as the color development intensity of the water absorption pad, and if the first difference value is smaller than or equal to 0, setting the color development intensity of the water absorption pad at the corresponding sampling moment to be 0.
5. A rapid detection system for atrazine screening in a food sample according to claim 1, wherein the method of obtaining an excess of detection bands comprises: For each sampling time, taking the sum value of the color development intensity of the detection band and the color development intensity of the water absorption pad as the excess of the detection band.
6. A rapid detection system for atrazine screening in a food sample according to claim 1, wherein the method of obtaining the extent of clogging comprises: The detection band-pass excess is used as a transverse axis, the color development intensity difference is used as a vertical axis, a two-dimensional coordinate system is constructed, data points in the two-dimensional coordinate system are divided into a first point set and a second point set according to the transverse axis, the second point set is arranged behind the first point set on the transverse axis, and the variation trend difference of the data points between the second point set and the first point set is analyzed to obtain the blocking growth degree.
7. A rapid detection system for atrazine screening in a food sample according to claim 1, wherein the occlusion detection is performed in sequence based on the detection band excess at the final time, the detection band color intensity, and the occlusion increase level, and comprising a three-stage detection process comprising: the first-stage detection process, wherein if the detection band-pass excess under the final moment is smaller than a preset throughput threshold, the feedback detection is invalid, otherwise, the second-stage detection process is carried out; A secondary detection process, namely feeding back a negative detection result if the color development intensity of the detection zone at the final moment is not less than a preset color development intensity threshold value, otherwise, entering a tertiary detection process; And in the three-stage detection process, if the blocking growth degree is not smaller than a preset blocking threshold value, feeding back a false positive detection result, and otherwise, feeding back a positive detection result.
8. A rapid test system for atrazine screening in a food sample according to claim 1, wherein the dividing the test strip region into an upstream region and a downstream region comprises: And constructing an area to be analyzed according to a preset analysis size by taking the T line position as a center, wherein the T line position divides the area to be analyzed into the upstream area and the downstream area.
9. The rapid detection system for atrazine screening in a food sample according to claim 1, wherein the method for obtaining the difference in chromogenic intensity comprises: subtracting the color development intensity of the downstream region from the color development intensity of the upstream region to obtain the color development intensity difference.
10. A rapid detection system for atrazine screening in a food sample, according to claim 6, wherein the analysis of the difference in trend of change in data points between the second set of points and the first set of points, to obtain the degree of blockage growth, comprises: The method comprises the steps of obtaining a first point set, obtaining a second point set, obtaining a first fitting slope by linearly fitting the data points in the first point set, obtaining a second fitting slope by linearly fitting the data points in the second point set, and taking the difference value of subtracting the first fitting slope from the second fitting slope as the occlusion growth degree.

Description

Rapid detection system for atrazine screening in food samples Technical Field The invention relates to the technical field of material detection, in particular to a rapid detection system for atrazine screening in food samples. Background Agricultural food materials such as corn steep liquor and sugarcane juice often contain residual herbicide such as atrazine. In situ rapid screening for such samples relies primarily on competition-based lateral flow immunochromatographic techniques. In the competition method detection mode, target small molecules in the sample compete with the gold-labeled antibody for binding to the antigen on the detection zone, and the lighter the T line (detection zone) is developed, the higher the concentration of the target in the sample is represented. However, practical food matrices typically have high viscosity and contain a large amount of suspended particles. These tiny particles, when flowing through the porous chromatographic membrane, create a depth filtration effect, resulting in gradual packing of the membrane pores. This non-specific physical obstruction can impede fluid transport, resulting in a reduction in the total amount of gold-labeled complex reaching the detection zone, as well as a lightening of the T-line. Existing optical detection devices generally only read a single gray value of the reaction endpoint, and cannot distinguish whether the weak color development is caused by the chemical competition inhibition of atrazine or by the physical blockage of matrix particles. This limitation results in the easy misjudgment of the reduction of the signal caused by physical blockage as atrazine exceeding in the detection of high-viscosity food samples, thereby generating false positive results and affecting the screening accuracy. Disclosure of Invention In order to solve the technical problems that the prior art cannot analyze the influence of particle physical blockage on the atrazine detection result and further cause inaccurate detection, the invention aims to provide a rapid detection system for atrazine screening in a food sample, and the adopted technical scheme is as follows: The invention provides a rapid detection system for atrazine screening in a food sample, which comprises: the optical data acquisition module is used for continuously acquiring a plurality of test paper images at the moment of liquid contact, wherein the test paper images comprise a detection zone area image, a test paper background area image and a water absorption pad area image; the flow data quantization module is used for respectively removing the background gray value of the test paper background area image from the gray value in the detection area image and the water absorption pad area image under each sampling time to sequentially obtain the color development intensity of the detection area and the color development intensity of the water absorption pad; The detection zone material change analysis module is used for dividing the detection zone into an upstream zone and a downstream zone, and obtaining the color development intensity difference between the color development intensity of the upstream zone and the color development intensity of the downstream zone; And the color development detection module is used for carrying out blocking detection according to the detection band excess, the detection band color development intensity and the blocking growth degree at the final moment in sequence. Further, the method for obtaining the background gray value comprises the following steps: And for each sampling time, taking the average gray value in the background area image of the test paper as the background gray value at the corresponding sampling time. Further, the method for obtaining the color development intensity of the detection zone comprises the following steps: and for each sampling moment, obtaining a first difference value of subtracting the average gray value in the detection zone region image from the background gray value, if the first difference value is larger than 0, taking the first difference value as the color development intensity of the detection zone, and if the first difference value is smaller than or equal to 0, setting the color development intensity of the detection zone at the corresponding sampling moment to be 0. Further, the method for obtaining the color development intensity of the water absorption pad comprises the following steps: And for each sampling moment, obtaining a second difference value of the background gray value minus the average gray value in the image of the water absorption pad area, if the second difference value is larger than 0, taking the first difference value as the color development intensity of the water absorption pad, and if the first difference value is smaller than or equal to 0, setting the color development intensity of the water absorption pad at the corresponding sampling moment to be 0. Further, the method for acqui