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CN-122016936-A - Plant root system controllable uniform electromagnetic field platform and electromagnetic field exposure experiment method

CN122016936ACN 122016936 ACN122016936 ACN 122016936ACN-122016936-A

Abstract

The invention relates to the technical field of microfluidics, and discloses a plant root system controllable uniform electromagnetic field platform and an electromagnetic field exposure experiment method, wherein the platform comprises a Helmholtz coil assembly, a magnetic field sensor and a magnetic field sensor, wherein the Helmholtz coil assembly is used for generating a uniform electromagnetic field in a central area; the micro-fluidic chip culture unit is arranged in a coil central working area, the chip is provided with a seed inlet, a root system growth channel and a culture solution inlet and outlet, the current regulation and control unit is electrically connected with the coil and outputs controllable direct current, and the control unit is electrically connected with the current regulation and control unit, receives an electromagnetic field parameter instruction and converts the electromagnetic field parameter instruction into a current control signal. The method comprises seed inoculation culture, chip placement coil workbench, parameter setting and current output, electromagnetic field exposure and in-situ observation record. The invention integrates microfluidic visual culture and Helmholtz coil uniform magnetic field, digitally and accurately regulates and controls magnetic field intensity, and solves the problems of uneven field intensity and poor repeatability of the traditional device.

Inventors

  • ZHANG HUALI
  • WANG YUJIA
  • YAN XU
  • WANG KAIJIN

Assignees

  • 南通大学

Dates

Publication Date
20260512
Application Date
20260227

Claims (10)

  1. 1. The utility model provides a controllable even electromagnetic field platform of plant root system which characterized in that includes: a magnetic field generating unit (1), wherein the magnetic field generating unit (1) adopts a Helmholtz coil assembly and is used for generating an electromagnetic field which is uniformly distributed in a central area of the Helmholtz coil assembly; The micro-fluidic chip culture unit (2) is arranged in the central working area of the Helmholtz coil assembly and is used for accommodating plant root systems and enabling root system growth channels to be completely arranged in the coverage range of the uniform electromagnetic field; The output end of the current regulation unit (3) is electrically connected with the power input end of the Helmholtz coil assembly and used for outputting controllable direct current to the Helmholtz coil assembly so as to regulate the intensity of an electromagnetic field; The signal output end of the control unit (4) is electrically connected with the signal input end of the current regulation and control unit (3) and is used for receiving an externally set electromagnetic field parameter instruction and converting the electromagnetic field parameter instruction into a corresponding current control signal so as to drive the current regulation and control unit (3) to output direct current with corresponding size.
  2. 2. The plant root system controllable uniform electromagnetic field platform according to claim 1, wherein the helmholtz coil assembly is a one-dimensional helmholtz coil (11) comprising two identical, coaxially parallel arranged circular coils.
  3. 3. The plant root system controllable uniform electromagnetic field platform according to claim 1 or 2, wherein the microfluidic chip cultivation unit (2) comprises: the chip comprises a chip body (21), wherein the chip body (21) is provided with at least one seed inlet (211), a root growth channel communicated with the seed inlet (211) and a culture solution inlet and outlet (212); the extension path of the root system growth channel is positioned in the projection range of the center working area of the Helmholtz coil assembly.
  4. 4. A plant root system controllable uniform electromagnetic field platform according to claim 3, wherein the microfluidic chip culture unit (2) further comprises a culture flask, the chip body (21) being sealingly arranged in the culture flask, the culture flask being arranged on a central table (12) of the helmholtz coil assembly.
  5. 5. A plant root system controllable uniform electromagnetic field platform according to claim 1, wherein the control unit (4) comprises: The man-machine interaction module is used for receiving electromagnetic field parameters input by a user; The singlechip control core is in communication connection with the man-machine interaction module and is used for converting the electromagnetic field parameters into current control signals; And the communication interface is used for realizing data transmission between the man-machine interaction module and the singlechip control core.
  6. 6. The plant root system controllable uniform electromagnetic field platform of claim 5, wherein the human-computer interaction module is a computer, and the communication interface is a serial port or a USB interface.
  7. 7. The plant root system controllable uniform electromagnetic field platform according to claim 1, wherein the current regulation unit (3) is a programmable direct current power supply or a digital current source.
  8. 8. The plant root system controllable uniform electromagnetic field platform according to claim 1, further comprising a climate box, wherein the microfluidic chip culturing unit (2) is arranged in the climate box, and the climate box is used for providing temperature, humidity and illumination environment for plant root system growth.
  9. 9. The plant root system electromagnetic field exposure experiment method is characterized by comprising the following steps of: Placing plant seeds into a seed inlet (211) of the microfluidic chip, introducing a culture solution, and placing the chip in a climatic box for culturing until the seeds germinate and the root system extends to a growth channel; Placing a microfluidic chip culture unit (2) containing germinated seeds on a central workbench (12) of a Helmholtz coil assembly; The control unit (4) is used for inputting target electromagnetic field parameters, the control unit (4) is used for converting the parameters into current control signals, and the current regulation and control unit (3) is driven to output direct current with corresponding magnitude to the Helmholtz coil assembly; the Helmholtz coil component generates a uniform electromagnetic field in a central area and acts on plant root systems in the microfluidic chip; in the electromagnetic field action process, the plant root system is observed and recorded in situ through the microfluidic chip.
  10. 10. The method of plant root electromagnetic field exposure assay of claim 9, wherein: In the step of inputting a target electromagnetic field parameter through the control unit (4), converting the parameter into a current control signal by the control unit (4), and driving the current regulation and control unit (3) to output direct current with a corresponding magnitude to the Helmholtz coil assembly, the electromagnetic field parameter comprises one or more of magnetic field intensity and application duration; in the in-situ observation and recording steps of plant root systems through the microfluidic chip in the electromagnetic field action process, a control group is synchronously arranged, the growth state of the plant root systems under the electromagnetic field-free condition is observed and recorded, and comparison analysis is carried out with an experimental group.

Description

Plant root system controllable uniform electromagnetic field platform and electromagnetic field exposure experiment method Technical Field The invention relates to the technical field of microfluidics, in particular to a plant root system controllable uniform electromagnetic field platform and a plant root system electromagnetic field exposure experiment method. Background The plant root system is a core organ for regulating and controlling nutrient absorption, signal transmission and growth and development, and the association of the physiological characteristics and the external physical environment is a research hot spot in the fields of plant biology and agricultural physical regulation. Electromagnetic fields have a long history of research on plant applications and remain an attractive area of research for developing strategies for controlling plant growth. The method is used for accurately analyzing the influence of an electromagnetic field on root system morphogenesis and physiological metabolism, and is an important basis for developing novel electric plant stimulation technology and optimizing crop cultivation environment. The field intensity of the traditional device is unevenly distributed in space, the field receiving difference of different areas of the plant root system is large, and the experimental repeatability and accuracy are poor. Disclosure of Invention The invention provides a plant root system controllable uniform electromagnetic field platform and an electromagnetic field exposure experiment method, which are used for solving the problems. In a first aspect, the present invention provides a plant root system controllable uniform electromagnetic field platform, comprising: the magnetic field generation unit adopts a Helmholtz coil assembly and is used for generating an evenly distributed electromagnetic field in a central area of the Helmholtz coil assembly; the microfluidic chip culture unit is arranged in the central working area of the Helmholtz coil assembly and is used for accommodating plant root systems and enabling root system growth channels to be completely arranged in the coverage range of the uniform electromagnetic field; the output end of the current regulation unit is electrically connected with the power input end of the Helmholtz coil assembly and used for outputting controllable direct current to the Helmholtz coil assembly so as to regulate the intensity of an electromagnetic field; The signal output end of the control unit is electrically connected with the signal input end of the current regulation and control unit and is used for receiving an externally set electromagnetic field parameter instruction and converting the electromagnetic field parameter instruction into a corresponding current control signal so as to drive the current regulation and control unit to output direct current with corresponding size. According to the platform provided by the invention, the micro-fluidic chip culture unit is accurately arranged in the uniform magnetic field area of the Helmholtz coil assembly, and a digital closed-loop regulation and control link is formed by the control unit and the current regulation and control unit, so that the accurate control and repeatability guarantee of the plant root system electromagnetic field exposure experiment are realized. The inherent magnetic field uniformity characteristic of the Helmholtz coil assembly ensures that root systems at different space positions in a chip are subjected to the action of an equal-intensity electromagnetic field, and fundamentally solves the problem of experimental deviation caused by uneven field intensity distribution in the traditional experimental device. In an alternative embodiment, the helmholtz coil assembly is a one-dimensional helmholtz coil comprising two identical, coaxially parallel arranged circular coils. In an alternative embodiment, the microfluidic chip culturing unit includes: The chip body is provided with at least one seed inlet, a root growth channel communicated with the seed inlet and a culture solution inlet and outlet; the extension path of the root system growth channel is positioned in the projection range of the center working area of the Helmholtz coil assembly. In an alternative embodiment, the microfluidic chip culturing unit further comprises a culture flask, the chip body is hermetically placed in the culture flask, and the culture flask is placed on a center workbench of the helmholtz coil assembly. In an alternative embodiment, the control unit comprises: The man-machine interaction module is used for receiving electromagnetic field parameters input by a user; The singlechip control core is in communication connection with the man-machine interaction module and is used for converting the electromagnetic field parameters into current control signals; And the communication interface is used for realizing data transmission between the man-machine interaction module and the singl