CN-122016962-A - Application of potential sensor based on polyaniline modified miniature two-electrode system in detection of methylmalonic acid

Abstract

The invention belongs to the field of medicine analysis and detection, and particularly relates to application of a potential sensor based on a polyaniline modified miniature two-electrode system to detection of methylmalonic acid. In the potential sensor based on the polyaniline modified micro two-electrode system, carbon fiber loaded with polyaniline is used as a working electrode, and an open-circuit potential method is adopted to detect the methylmalonic acid, so that the potential sensor is particularly suitable for detecting the methylmalonic acid in body fluid or excrement, is free from pretreatment, is instant, quick and accurate in detection, has strong anti-interference capability and high sensitivity, and provides a novel efficient and reliable analysis method for detecting the methylmalonic acid.

Inventors

• LIU YIMAN
• WANG HAN
• ZHANG ZHONGBO
• WU DONGQING
• LU CHENG
• Yin Haize

Assignees

• 上海工程技术大学

Dates

Publication Date

20260512

Application Date

20260123

Claims (10)

1. 1. The application of the potential sensor based on the polyaniline modified micro two-electrode system for detecting the methylmalonic acid is characterized in that the potential sensor based on the polyaniline modified micro two-electrode system uses carbon fiber loaded with polyaniline as a working electrode and adopts an open circuit potential method for detecting the methylmalonic acid.
2. 2. The use according to claim 1, characterized in that an Ag/AgCl electrode is used as reference electrode.
3. 3. The method according to claim 1, wherein the polyaniline-loaded carbon fiber electrode is prepared by oxidizing aniline into polyaniline in situ by cyclic voltammetry in an electrolyte solution formed by aniline, hydrochloric acid and deionized water, and loading the polyaniline on the surface of the carbon fiber by using a three-electrode system.
4. 4. Use according to claim 3, characterized in that in the three-electrode system carbon fibres are used as working electrode, ag/AgCl electrode as reference electrode and platinum wire electrode as counter electrode.
5. 5. The use according to claim 4, wherein the carbon fibers are ultrasonically cleaned with ethanol and water, respectively.
6. 6. The use according to claim 3, wherein the cyclic voltammetry scans not less than 8 turns over the range +0.2V to +1.2V.
7. 7. The use according to claim 3, wherein the concentration of aniline in the electrolyte solution is 0.08-0.35M and the concentration of hydrochloric acid is 0.08-0.2M.
8. 8. The electrochemical detection method of the methylmalonic acid is characterized by comprising the steps of detecting the methylmalonic acid in a sample liquid to be detected by using a polyaniline-modified miniature two-electrode system-based potential sensor and taking polyaniline-loaded carbon fibers as working electrodes through an open-circuit potential method by an external standard or an internal standard.
9. 9. The electrochemical detection method of claim 8, wherein an Ag/AgCl electrode is used as the reference electrode.
10. 10. The electrochemical detection method according to claim 8, wherein the sample liquid to be detected is selected from any one of a body fluid, an excreta, a dilution of a body fluid, or a dilution of an excreta.

Description

Application of potential sensor based on polyaniline modified miniature two-electrode system in detection of methylmalonic acid Technical Field The invention belongs to the field of medicine analysis and detection, and particularly relates to application of a potential sensor based on a polyaniline modified miniature two-electrode system to detection of methylmalonic acid. Background Methylmalonate (Methylmalonic acid, MMA) is a key blood or urine metabolic marker for diagnosing hereditary metabolic diseases such as methylmalonate blood. Currently, detection mainly depends on methods such as gas chromatography-mass spectrometry (GC-MS), high Performance Liquid Chromatography (HPLC), genetic gene detection and the like. For example, CN117929564A is a method for rapidly and quantitatively detecting methylmalonic acid in a biological sample based on liquid chromatography-mass spectrometry, CN115508483a is a LC-MS/MS method for rapidly detecting methylmalonic acid in serum samples, CN112834682A is a method for detecting methylmalonic acid content in blood, CN104991018a is a urine treatment method and a gas chromatography-mass spectrometry method for detecting methylmalonic acid in urine, CN101680904A uses a high-performance stationary phase HILIC and mass spectrometry for quantitatively detecting methylmalonic acid and succinic acid, CN120843667a is a probe combination and detection device for detecting methylmalonic acid disease pathogenic genes, CN120683245A combined methylmalonic acid disease detection kit, CN114457148a simple methylmalonic acid disease gene mutation detection primer pair, kit and the like, and the methods have high accuracy, but generally rely on large-scale instruments, the pretreatment of samples is complex and time-consuming, and the requirement of clinical immediate detection is difficult to be satisfied. The potentiometric electrochemical sensor is considered as a potential platform for realizing the instant detection of MMA because of its simple equipment, rapid response and easy miniaturization. Among them, sensors based on materials such as conductive polymer Polyaniline (PANI) are attracting attention due to their good stability and ion-electron conversion characteristics. However, the existing MMA detection scheme based on the potential sensing principle still has the limitation that first, the system integration level is insufficient. The traditional three-electrode system is adopted in most of the sensor, the volume of a conventional reference electrode is large, the overall miniaturization and the integrated portability of the sensor are restricted, and second, the selectivity in complex physiological samples is poor. For a real sample containing high-concentration electrolyte (such as Na +、K+、NH4+) and an interfering substance such as urea, the sensor is easily interfered by ions, so that the sensitivity and the specificity are reduced. Therefore, how to construct a potential sensor which has simple structure, quick response and high sensitivity and can be directly used for in-situ detection of complex real samples becomes a technical problem to be solved at present. The open circuit potentiometric method (OCPT) provides a potential solution to the above problems as a simplified potentiometric strategy. Disclosure of Invention The invention provides a potential sensor based on a polyaniline modified micro two-electrode system, which can directly detect methyl malonic acid in complex real samples such as body fluid or excrement, has no need of pretreatment, and has the advantages of rapid and accurate detection, strong anti-interference capability and high sensitivity. According to the technical scheme, the application of the electric potential sensor based on the polyaniline modified micro-electrode system in detecting the methylmalonic acid is realized, wherein in the electric potential sensor based on the polyaniline modified micro-electrode system, polyaniline-loaded carbon fibers are used as working electrodes, ag/AgCl electrodes are used as reference electrodes, and an open circuit electric potential method is adopted for detecting the methylmalonic acid, so that the electric potential sensor is particularly suitable for quantitatively detecting the methylmalonic acid. The preparation method of the polyaniline-loaded carbon fiber electrode comprises the steps of oxidizing aniline into polyaniline in situ by using a cyclic voltammetry in an electrolyte solution formed by aniline, hydrochloric acid and deionized water, and loading the polyaniline to the surface of carbon fibers. In the three-electrode system, carbon fiber is used as a working electrode, an Ag/AgCl electrode is used as a reference electrode, and a platinum wire electrode is used as a counter electrode. The carbon fibers are respectively subjected to ultrasonic cleaning by ethanol and water. The cyclic voltammetry scans not less than 8 cycles, preferably not less than 9 cycles, more preferably 10 to 15 cycles