CN-122016963-A - Aflatoxin electrochemical sensor and preparation method and application thereof

Abstract

The invention belongs to the technical field of sensors, and discloses an aflatoxin electrochemical sensor, a preparation method and application thereof. The aflatoxin electrochemical sensor comprises a nano graphite carbon paste electrode and a composite film loaded on the nano graphite carbon paste electrode, wherein the composite film comprises a perfluorosulfonic acid ion exchange polymer and a cationic quaternary ammonium salt surfactant. The aflatoxin electrochemical sensor provided by the invention has the advantages of low preparation cost, simplicity in operation, capability of rapidly and accurately measuring aflatoxin B1, and good application prospect.

Inventors

• ZHENG DONGYUN
• HUANG SIYU
• LI HONG
• HE RONG
• MA JUAN
• HU XIAOJING
• Xia Tingjuan

Assignees

• 中南民族大学
• 重庆市食品药品检验检测研究院
• 武汉食品化妆品检验所

Dates

Publication Date

20260512

Application Date

20260127

Claims (10)

1. 1. An aflatoxin electrochemical sensor is characterized by comprising a nano graphite carbon paste electrode and a composite film loaded on the nano graphite carbon paste electrode; The composition of the composite membrane comprises a perfluorinated sulfonic acid ion exchange polymer and a cationic quaternary ammonium salt surfactant.
2. 2. The aflatoxin electrochemical sensor according to claim 1, wherein the mass ratio of the perfluorosulfonic acid ion-exchange polymer to the cationic quaternary ammonium surfactant in the composite membrane is 1 (1-4).
3. 3. The aflatoxin electrochemical sensor of claim 1, wherein the cationic quaternary ammonium surfactant comprises cetyltrimethylammonium bromide.
4. 4. A method for preparing an aflatoxin electrochemical sensor according to any of claims 1-3, comprising the steps of: Immersing the nano graphite carbon paste electrode into a solution containing a perfluorosulfonic acid ion exchange polymer and a cationic quaternary ammonium salt surfactant, and then scanning by adopting a cyclic voltammetry to prepare the aflatoxin electrochemical sensor.
5. 5. The method according to claim 4, wherein the mass concentration of the perfluorosulfonic acid ion exchange polymer in the solution containing the perfluorosulfonic acid ion exchange polymer and the cationic quaternary ammonium surfactant is 0.01 to 0.1%.
6. 6. The method according to claim 4, wherein the concentration of the cationic quaternary ammonium salt surfactant in the solution containing the perfluorosulfonic acid ion exchange polymer and the cationic quaternary ammonium salt surfactant is 1-5 mmol/L.
7. 7. The method according to claim 4, wherein the scanning rate by cyclic voltammetry is 80-120 mV/s.
8. 8. The method of claim 4, wherein the cyclic voltammetry is used for scanning at a voltage in the range of-1.6V to 1.6V.
9. 9. The method according to claim 4, wherein the number of turns of the scan by cyclic voltammetry is 5 to 20.
10. 10. Use of an aflatoxin electrochemical sensor according to any of claims 1-3 in the field of food safety or environmental monitoring.

Description

Aflatoxin electrochemical sensor and preparation method and application thereof Technical Field The invention belongs to the technical field of sensors, and particularly relates to an aflatoxin electrochemical sensor, a preparation method and application thereof. Background Aflatoxin B1 (Aflatoxins B1, AFB 1) is the most common and most carcinogenic, listed as a class 1 carcinogen by the international agency for research on cancer (IARC). AFB1 is widely found in a variety of foods such as cereals, peanuts, milk, fruits and meats. The toxin has stable physicochemical properties, and toxicity may be further enhanced in the metabolic process after being ingested by a food chain, and can cause serious diseases such as liver hemorrhage, liver cancer and the like, and the toxicological mechanism of the toxin is reported in the literature. Currently, various methods for detecting AFB1 have been reported, mainly including High Performance Liquid Chromatography (HPLC), surface Plasmon Resonance (SPR), enzyme-linked immunosorbent assay (ELISA), surface Enhanced Raman Scattering (SERS), and Thin Layer Chromatography (TLC). However, these methods have certain drawbacks, such as High Performance Liquid Chromatography (HPLC), surface Plasmon Resonance (SPR), enzyme-linked immunosorbent assay (ELISA), surface Enhanced Raman Scattering (SERS), and Thin Layer Chromatography (TLC), which have drawbacks of complex methods, complex sample pretreatment techniques, expensive instruments, and long time consumption, which limit their wide application in high throughput and in-situ sample analysis. Therefore, developing a method for detecting aflatoxin B1 with low cost, simplicity, rapidness and accuracy is of great importance for human and animal health. Disclosure of Invention The present invention aims to solve at least one of the technical problems in the prior art described above. Therefore, the invention provides an aflatoxin electrochemical sensor, a preparation method and application thereof. The aflatoxin electrochemical sensor provided by the invention has the advantages of low preparation cost, simplicity in operation, capability of rapidly and accurately measuring aflatoxin B1, and good application prospect. A first aspect of the invention provides an aflatoxin electrochemical sensor. An aflatoxin electrochemical sensor comprises a nano graphite carbon paste electrode and a composite film loaded on the nano graphite carbon paste electrode; The composition of the composite membrane comprises a perfluorinated sulfonic acid ion exchange polymer and a cationic quaternary ammonium salt surfactant. Preferably, in the composite membrane, the mass ratio of the perfluorinated sulfonic acid ion exchange polymer to the cationic quaternary ammonium salt surfactant is 1 (1-4). For example 1:2. Preferably, the cationic quaternary ammonium surfactant comprises cetyl trimethylammonium bromide (CTAB). The second aspect of the invention provides a preparation method of the aflatoxin electrochemical sensor. A preparation method of an aflatoxin electrochemical sensor comprises the following steps: Immersing the nano graphite carbon paste electrode into a solution containing a perfluorosulfonic acid ion exchange polymer (Nafion) and a cationic quaternary ammonium salt surfactant, and then scanning by adopting a Cyclic Voltammetry (CV) method to prepare the aflatoxin electrochemical sensor. Preferably, the mass concentration of the perfluorinated sulfonic acid ion exchange polymer in the solution containing the perfluorinated sulfonic acid ion exchange polymer and the cationic quaternary ammonium salt surfactant is 0.01-0.1%, and more preferably 0.04-0.06%. Preferably, the concentration of the cationic quaternary ammonium salt surfactant in the solution containing the perfluorinated sulfonic acid ion exchange polymer and the cationic quaternary ammonium salt surfactant is 1-5 mmol/L. For example, 2-4 mmol/L. Preferably, the scanning is performed at a rate of 80-120 mV/s, more preferably 90-100 mV/s, using Cyclic Voltammetry (CV). Preferably, the voltage range for scanning by Cyclic Voltammetry (CV) is from-1.6V to 1.6V. Preferably, the number of turns scanned by Cyclic Voltammetry (CV) is 5-20, more preferably 10-12. Preferably, the process of scanning using cyclic voltammetry is performed using an electrochemical workstation. Preferably, after the scanning is finished, the method further comprises a cleaning and drying process. Preferably, the washing is performed using distilled water. Preferably, the drying process is natural drying at room temperature for 5-10 minutes. A third aspect of the invention provides the use of an aflatoxin electrochemical sensor. The aflatoxin electrochemical sensor is applied to the field of food safety or environmental monitoring. Compared with the prior art, the invention has the following beneficial effects: (1) The invention constructs a composite membrane on a nano graphite carbon paste electrode, wherein