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CN-122016970-A - CuO/Cu(OH)2-Ni(OH)2Ni electrode and application thereof in passion fruit juice detection

CN122016970ACN 122016970 ACN122016970 ACN 122016970ACN-122016970-A

Abstract

The invention provides a CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode and application thereof in passion fruit juice detection, wherein the electrode is prepared by pretreating a nickel electrode, placing the treated nickel electrode in a mixed solution containing hydrochloric acid and cupric chloride, carrying out electrochemical deposition by a square wave pulse method to form a deposition layer on the surface of the nickel electrode, placing the obtained electrode in an alkaline solution containing sodium hydroxide and glucose, and carrying out electrochemical activation treatment by a cyclic voltammetry to obtain the CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode. The electrode has excellent electrocatalytic oxidation performance on glucose, has wide linear detection range, high sensitivity and low detection limit, has good anti-interference performance, repeatability and long-term stability, and can realize rapid and accurate quantitative detection of the glucose in the passion fruit juice substrate.

Inventors

  • CAO XIAOHUANG
  • LIU XING
  • YANG NA
  • JIN YAMEI
  • ZHANG BIAOMING
  • WAN JUNXI
  • CHEN FUHUA
  • Pei Yuanjiao
  • Chen Zhongsuzhi
  • CHEN ZHILIN
  • HAN CONGYING
  • He Silian
  • LI NA
  • ZHANG TIANYU

Assignees

  • 玉林师范学院
  • 江南大学

Dates

Publication Date
20260512
Application Date
20260331

Claims (10)

  1. 1. The preparation method of the CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode is characterized by comprising the following steps: step S1, preprocessing a nickel electrode to obtain a clean nickel electrode; Step S2, placing the nickel electrode treated in the step S1 into a mixed solution containing hydrochloric acid and copper chloride, and performing electrochemical deposition on the surface of the nickel electrode by a square wave pulse method to form a deposition layer, wherein the concentration of hydrochloric acid in the mixed solution is 0.05-2.0 mol/L, the concentration of copper chloride is 0.05-2.0 mol/L, the potential range of the square wave pulse method is-0.6V-0V, and the deposition time is 20-200 seconds; And S3, placing the electrode obtained in the step S2 into an alkaline solution containing sodium hydroxide and glucose, and performing electrochemical activation treatment by adopting a cyclic voltammetry to obtain the CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode, wherein the concentration of the sodium hydroxide in the alkaline solution is 0.05-0.2 mol/L, and the concentration of the glucose in the alkaline solution is 0.1-10 mmol/L.
  2. 2. The method for preparing a CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode according to claim 1, further comprising the step of S4, soaking the obtained CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode in deionized water for 5-10 min to wash off residual liquid on the surface of the electrode, performing ultrasonic treatment for 5-20S by an ultrasonic cleaner, taking out and airing.
  3. 3. The preparation method of the CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode according to claim 1, wherein the pretreatment in the step S1 comprises polishing a nickel electrode by sand paper, polishing the nickel electrode to a mirror surface by aluminum oxide powder on a polishing cloth, and finally performing ultrasonic cleaning in distilled water, wherein in the step S2, the concentration of hydrochloric acid is 0.1-1 mol/L, the concentration of cupric chloride is 0.1-0.5 mol/L, and the deposition time is 50-100 seconds.
  4. 4. The method for producing a CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode according to claim 3, wherein in step S2, the concentration of hydrochloric acid is 0.1 mol/L, the concentration of cupric chloride is 0.3 mol/L, and the deposition time is 100 seconds.
  5. 5. The method for producing a CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode according to claim 1, wherein in step S3, the cyclic voltammetry is performed at a scanning rate of 10 to 150 mV/S and a scanning number of1 to 30 cycles.
  6. 6. The method for producing a CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode according to claim 5, wherein in step S3, the concentration of sodium hydroxide is 0.1: 0.1 mol/L, the concentration of glucose is 10: 10 mmol/L, the sweep rate by cyclic voltammetry is 50: 50 mV/S, and the number of turns is 10.
  7. 7. A CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode, wherein the CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode according to any one of claims 1 to 6 is prepared.
  8. 8. The CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode according to claim 7, wherein the CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode has a porous structure composed of nanoparticles, and the electrode surface contains Ni (OH) 2 , cuO and Cu (OH) 2 as active components.
  9. 9. Use of the CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode according to claim 7 or 8 in passion fruit juice detection.
  10. 10. A method for detecting the content of glucose in passion fruit juice is characterized in that the CuO/Cu (OH) 2 -Ni(OH) 2 /Ni electrode as claimed in claim 7 or 8 is used as a working electrode, and an electrochemical analysis method is used for quantitatively detecting the glucose in passion fruit juice samples.

Description

CuO/Cu (OH) 2-Ni(OH)2/Ni electrode and application thereof in passion fruit juice detection Technical Field The invention relates to the technical field of electrochemical sensors, in particular to a CuO/Cu (OH) 2-Ni(OH)2/Ni electrode and application thereof in passion fruit juice detection. Background The passion fruit is used as a tropical and subtropical special fruit, is rich in various nutritional ingredients, and the quality of the juice is closely related to the content of glucose. Glucose is the main soluble sugar in the passion fruit juice, and the content directly determines the sweetness, flavor and nutritive value of the fruit juice, and is a core index of fruit juice quality grading, process optimization and adulteration identification. Therefore, the establishment of a rapid, accurate and sensitive glucose detection method has important practical significance. Currently, the methods for detecting glucose mainly comprise high performance liquid chromatography, gas chromatography, spectrophotometry and electrochemical sensor methods. The method has the advantages of high accuracy, complicated sample pretreatment, long detection period, high instrument cost and the like, and the spectrophotometry has simple and convenient operation and limited selectivity and sensitivity. The electrochemical sensor method is an ideal technical scheme for detecting glucose because of the advantages of quick response, convenient operation, low cost, high sensitivity, easy microminiaturization and the like. Among electrochemical sensors, enzyme-free glucose sensors are receiving attention because of their high stability and low cost. Among them, nickel-based and copper-based materials have been studied extensively for their good catalytic performance. However, the catalytic activity and stability of the single component electrode material remain to be improved. In addition, the components of fructose, glucose, polyphenol, pigment and the like in the passion fruit juice are complex, and competition adsorption or side reaction is easy to occur between the electrode surface and ascorbic acid, so that the sensor selectivity is reduced. In addition, the existing material has insufficient exposure of active sites in dimensional matching and structural design, and has difficult compatibility between conductivity and catalytic performance, so that the rapid and sensitive detection of low-concentration targets in complex matrixes is restricted. Therefore, the composite electrode material which has the advantages of simple preparation process, high sensitivity, good stability, strong anti-interference capability, simple operation and reliable result is developed, and is applied to the rapid and accurate detection of glucose in complex matrixes (if juice), so that the composite electrode material has important application value. Disclosure of Invention Aiming at the technical problems, the invention discloses a CuO/Cu (OH) 2-Ni(OH)2/Ni electrode and application thereof in passion fruit juice detection, the electrode has wide linear detection range, high sensitivity and low detection limit, and is excellent in anti-interference performance, stability, repeatability and reproducibility, the technical problems that the sensitivity, stability and anti-interference capability of a glucose detection electrode in the prior art are to be improved are solved, and the rapid and accurate quantitative detection of glucose in passion fruit juice matrixes is realized. In this regard, the invention adopts the following technical scheme: a preparation method of a CuO/Cu (OH) 2-Ni(OH)2/Ni electrode comprises the following steps: step S1, preprocessing a nickel electrode to obtain a clean nickel electrode; Step S2, placing the nickel electrode treated in the step S1 into a mixed solution containing hydrochloric acid and copper chloride, and performing electrochemical deposition on the surface of the nickel electrode by a square wave pulse method to form a deposition layer, wherein the concentration of hydrochloric acid in the mixed solution is 0.05-2.0 mol/L, the concentration of copper chloride is 0.05-2.0 mol/L, the potential range of the square wave pulse method is-0.6V-0V, and the deposition time is 20-200 seconds; And S3, placing the electrode obtained in the step S2 into an alkaline solution containing sodium hydroxide and glucose, and performing electrochemical activation treatment by adopting a cyclic voltammetry to obtain the CuO/Cu (OH) 2-Ni(OH)2/Ni electrode, wherein the concentration of the sodium hydroxide in the alkaline solution is 0.05-0.2 mol/L, and the concentration of the glucose in the alkaline solution is 0.1-10 mmol/L. By adopting the technical scheme, a Cu (OH) 2 nanometer film is formed on the surface of the Ni electrode by a square wave pulse method, and then a CuO/Cu (OH) 2-Ni(OH)2/Ni electrode is successfully constructed on a nickel substrate by cyclic voltammetry activation. The electrode has a unique porous structur