CN-122016996-A - Electrospray ionization mass spectrometry imaging detection method for plastic additive on plastic surface

Abstract

The application discloses an electrospray ionization mass spectrometry imaging detection method of a plastic additive on a plastic surface, which belongs to the technical field of plastic additive detection and comprises the following steps of acquiring a standard substance map, processing a sample, acquiring data, processing the acquired data, converting mass spectrometry information distributed on the surface of a pretreated sample by a target plastic additive into image information according to the mass spectrometry map, analyzing the extracted image information, focusing on migration visualization analysis of the plastic additive, detecting the plastic additive only by simple steps without complex sample pretreatment, and having high sensitivity, detecting trace additives in the plastic, and carrying out DESI-MSI (deep-drawing-MSI) with the advantage of high flux.

Inventors

• JIANG JIE
• KAN GUANGFENG
• ZHANG YAN
• ZHANG HONG
• YU KAI
• JIANG YANXIAO
• WANG XIAOFEI
• JU YUN

Assignees

• 青岛哈尔滨工业大学(威海)研究院
• 哈尔滨工业大学（威海）

Dates

Publication Date

20260512

Application Date

20260206

Claims (10)

1. 1. The electrospray ionization mass spectrometry imaging detection method of the plastic additive on the plastic surface is characterized by comprising the following steps of: Firstly, obtaining a standard substance spectrum, namely carrying out mass spectrum detection on the plastic additive to obtain a primary mass spectrum and a secondary mass spectrum; Secondly, sample treatment, namely flushing a plastic sample to be detected, and airing to obtain a pretreated sample for later use; thirdly, data acquisition, namely performing electrospray ionization on the pretreated sample, and acquiring data to obtain electrospray ionization data; And fourthly, data processing analysis, namely processing the electrospray ionization data, converting mass spectrum information of the distribution of the target plastic additive on the surface of the pretreated sample into image information according to the primary mass spectrum and the secondary mass spectrum, and analyzing the extracted image information.
2. 2. The method for electrospray ionization mass spectrometry imaging detection of a plastic additive on a plastic surface according to claim 1, wherein the plastic additive in step (one) is triethyl phosphate, 2- (2H-benzotriazol-2-yl) -4, 6-di-tert-amylphenol, pentaerythritol tetrakis (3, 5-di-tert-butyl-4-hydroxyhydrocinnamate), diethyl phthalate, dioctyl phthalate, octyl methoxycinnamate, dimethyl phthalate, triethyl phosphate, acetyl tributyl citrate, or triisobutyl phosphate.
3. 3. The method for electrospray ionization mass spectrometry imaging detection of plastic additives on plastic surfaces according to claim 1, wherein in step (two), the plastic sample to be detected is rinsed with ultra-pure water.
4. 4. A method of electrospray ionization mass spectrometry imaging detection of a plastic additive as recited in claim 3, wherein the number of flushes is three.
5. 5. The method for detecting the electrospray ionization mass spectrum imaging of the plastic additive on the plastic surface according to claim 1, wherein in the step (III), a mass spectrometer is adopted for electrospray ionization, and a solvent adopted by the mass spectrometer is a mixed system of acetonitrile, water and ammonia water.
6. 6. The electrospray ionization mass spectrometry imaging detection method for the plastic additive on the plastic surface according to claim 5, wherein the volume ratio of acetonitrile to water to ammonia water is 5:4:1.
7. 7. The method for electrospray ionization mass spectrometry imaging detection of a plastic additive on a plastic surface according to claim 5, wherein the flow rate of the solvent is 5 μl/min.
8. 8. The method for electrospray ionization mass spectrometry imaging detection of a plastic additive as recited in claim 5, wherein the mass spectrometer parameters of step (three) are specifically as follows: Scanning mode, negative; Mass-to-charge ratio 50-1300 m/z; electrospray voltage 4.5 kV; Nitrogen pressure 0.4 Mpa.
9. 9. The electrospray ionization mass spectrometry imaging detection method for the plastic additive on the plastic surface, which is disclosed in claim 1, is characterized in that the specific step of processing the data in the step (four) is to import the data acquired in the step (three) into imzMLConvert software and convert the data into imzML format.
10. 10. The method of electrospray ionization mass spectrometry imaging detection of a plastic additive according to claim 1, wherein in step (four), mass spectrometry information is converted into image information by a MSIREADER procedure.

Description

Electrospray ionization mass spectrometry imaging detection method for plastic additive on plastic surface Technical Field The application belongs to the technical field of plastic additive detection, and particularly relates to an electrospray ionization mass spectrometry imaging detection method for a plastic additive on a plastic surface. Background In order to keep the stability of the plastic and improve the service performance of the plastic, various additives are added into the plastic, and the plastic additives are not combined with macromolecular polymers in a covalent bond mode except a few reactive additives, so the plastic additives are easy to migrate into a contact medium in the use process, the released plastic additives enter a human body along with micro plastic or directly in the form of the plastic additives in a feeding, breathing, skin contact mode and the like mode, participate in chemical-mediated biotoxicity, and the detection of the plastic additives on the surface of the plastic is of great significance for defining the migration path of the plastic additives from the plastic into the environment and further exploring the environmental toxicity of plastic products. The patent CN112229936B discloses a high-throughput analysis method for simultaneously detecting 33 plastic additives in food contact materials based on liquid chromatography-tandem mass spectrometry, which can detect and analyze the 33 plastic additives in 12 min, has the advantages of high sensitivity, better separation degree, short detection time and high detection efficiency, can meet the high-throughput detection requirement of various plastic additives in the food contact materials, but has the advantages of complex detection method, need of preparing standard solution, need of extracting samples with methanol to prepare sample solution, and needs of adding solvents to extract the plastic additives after homogenizing treatment of plastic samples by traditional methods such as GC-MS and HPLC, needs lengthy sample pretreatment and chromatographic separation steps, and is difficult to detect the plastic additives with low concentration due to the limitation of spatial resolution in infrared spectrum detection. Disclosure of Invention The application aims to provide an electrospray ionization mass spectrometry imaging detection method for plastic additives on the surface of plastics, which aims to solve the technical problems that the pretreatment of a plastic additive on the surface of plastics in the prior art for detecting samples is complex, and the migration process of the plastic additive on the surface can not be visualized. In order to achieve the aim, the technical scheme adopted by the application is that an electrospray ionization mass spectrometry imaging detection method of a plastic additive on a plastic surface is provided, which comprises the following steps: Firstly, obtaining a standard substance spectrum, namely carrying out mass spectrum detection on the plastic additive to obtain a primary mass spectrum and a secondary mass spectrum; Secondly, sample treatment, namely flushing a plastic sample to be detected, and airing to obtain a pretreated sample for later use; Thirdly, data acquisition, namely performing electrospray ionization on the pretreated sample, and acquiring data to obtain electrospray ionization data; and fourthly, data processing analysis, namely processing electrospray ionization data, converting mass spectrum information of the distribution of the target plastic additive on the surface of the pretreated sample into image information according to a primary mass spectrum and a secondary mass spectrum, and analyzing the extracted image information. In one of the embodiments of the present invention, The plastic additive in the step (one) is triethyl phosphate, 2- (2H-benzotriazole-2-yl) -4, 6-di-tert-amyl phenol, pentaerythritol tetra (3, 5-di-tert-butyl-4-hydroxy hydrocinnamate), diethyl phthalate, dioctyl phthalate, octyl methoxycinnamate, dimethyl phthalate, triethyl phosphate, acetyl tributyl citrate or triisobutyl phosphate. In one of the embodiments of the present invention, And (II) flushing the plastic sample to be tested by ultrapure water. In one of the embodiments of the present invention, The number of flushes was three. In one of the embodiments of the present invention, Electrospray ionization is carried out by adopting a mass spectrometer, and a solvent adopted by the mass spectrometer is a mixed system of acetonitrile, water and ammonia water. In one of the embodiments of the present invention, The volume ratio of acetonitrile, water and ammonia water is 5:4:1. In one of the embodiments of the present invention, The flow rate of the solvent was 5. Mu.l/min. In one of the embodiments of the present invention, The mass spectrometer parameters in step (three) are specifically as follows: Scanning mode, negative; Mass-to-charge ratio 50-1300 m/z; electrospray voltage 4.5