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CN-122017075-A - Method for detecting ratio of chiral inositol to inositol

CN122017075ACN 122017075 ACN122017075 ACN 122017075ACN-122017075-A

Abstract

The invention discloses a method for detecting the proportion of chiral inositol to inositol, which relates to the technical field of chiral inositol content detection, and comprises the steps of dissolving chiral inositol, inositol and water, preparing at least 5 standard substance solutions with different proportions of chiral inositol and same baume degree, recording the value of the baume degree of each standard substance solution as Be b‌ , measuring the optical rotation of each standard substance solution, ‌ drawing a standard curve by taking the proportion of the chiral inositol to the inositol as an abscissa and the optical rotation of the corresponding standard substance solution as an ordinate, measuring the baume degree and the optical rotation of a sample solution, recording the value of the baume degree of the sample solution as Be Y‌ , recording the optical rotation B of the sample solution as Be b according to a formula I when Be Y ≠Be b ; Formula I; substituting the optical rotation B into a standard curve to calculate the ratio of chiral inositol to inositol; the method is simple and quick, and has better accuracy.

Inventors

  • ZHU LIPING
  • HE DONGSHENG
  • JI XIN
  • CUI XIN
  • SHEN YINGYING
  • Cui Huolan

Assignees

  • 诸城市浩天药业有限公司

Dates

Publication Date
20260512
Application Date
20260310

Claims (10)

  1. 1. A method of detecting a chiral inositol to inositol ratio, the method comprising: Dissolving chiral inositol, inositol and water, and preparing at least 5 standard solution with different ratios of chiral inositol to inositol and same baume degree, wherein the value of baume degree of the standard solution is expressed as Be b‌ ; Measuring the optical rotation of each standard solution; ‌ drawing a standard curve by taking the ratio of chiral inositol to inositol in the standard solution as an abscissa and the optical rotation of the corresponding standard solution as an ordinate; Measuring the baume degree and the optical rotation of a sample solution, wherein the baume degree value of the sample solution is marked as Be Y‌ , and the optical rotation value of the sample solution is marked as A; Substituting the optical rotation of the sample solution into the standard curve when Be Y ＝Be b‌ to calculate the ratio of chiral inositol to inositol; When Be Y ≠Be b , calculating the optical rotation B of the sample solution when the Baume degree is Be b according to a formula I; Formula I; substituting optical rotation B into the standard curve to calculate the ratio of chiral inositol to inositol.
  2. 2. The method of claim 1, wherein the standard solution has a baume of 5-10%.
  3. 3. The method according to claim 1 or 2, characterized in that the content of chiral inositol is 10-90% based on the total mass of chiral inositol and inositol in the standard solution.
  4. 4. The method of claim 1 or 2, wherein the sample solution has a baume of 5-10%.
  5. 5. The method according to claim 1 or 2, wherein the temperature of each standard solution is the same, denoted as Tb, and Tb is 20 ℃ to 25 ℃ when the optical rotation of each standard solution is measured.
  6. 6. The method according to claim 5, wherein the temperature of the sample solution is Ti at 20 to 40 ℃ when the optical rotation of the sample solution is measured.
  7. 7. The method of claim 6, wherein when Be Y ＝Be b and Ti = Tb, substituting the optical rotation a of the sample solution into the standard curve calculates the chiral inositol to inositol ratio.
  8. 8. The method of claim 6, wherein when Be Y ＝Be b and Ti++ Tb ‌ , the optical rotation A Tb at the sample solution temperature Tb is calculated according to equation II, A Tb =A Ti - [ -0.00075× (Ti-Tb) ] formula II; Wherein, A Tb is the optical rotation value when the temperature of the sample solution is Tb, -0.00075 is the temperature coefficient, and A Ti is the optical rotation value when the temperature of the sample solution is Ti; The chiral inositol to inositol ratio was calculated by substituting optical rotation a Tb into the standard curve.
  9. 9. The method of claim 8, wherein when Be Y ≠Be b and ti+.tb ‌ , calculating the optical rotation a Tb for the sample solution at a temperature Tb according to formula II, substituting optical rotation a Tb into formula I to calculate the optical rotation B for the sample solution at a baume of Be b , substituting optical rotation B into the standard curve to calculate the chiral inositol to inositol ratio.
  10. 10. The method according to claim 1 or 2, wherein the sample solution is a production fluid for producing D-chiro-inositol by enzymatic conversion of inositol.

Description

Method for detecting ratio of chiral inositol to inositol Technical Field The invention relates to the technical field of chiral inositol content detection, in particular to a method for detecting the ratio of chiral inositol to inositol. Background D-chiro-inositol (DCI) is one of the nine isomers of inositol that has optical activity. In recent years, research has found that D-chiro-inositol (DCI) has not only the function of promoting liver lipid metabolism, but also special physiological functions such as insulin sensitization, blood sugar reduction, improvement of ovulation condition of polycystic ovary syndrome (PCOS) patients, hormone balance adjustment, menstrual disorder improvement, antioxidation, anti-aging, anti-inflammatory and the like. In a bioconversion way, D-chiro-inositol can be generated by enzymatic conversion of inositol, and in industrial production, the content of D-chiro-inositol needs to be detected in time by means of liquid chromatography analysis, so that the material conversion condition is checked, whether a process can be downstream or not is determined, time is consumed, a liquid phase detection instrument is occupied, and the production cannot be separated from detection of the eyes. Therefore, there is a need to establish a simple and rapid quantitative analysis method for determining the content of chiral inositol in a feed solution. Disclosure of Invention The invention aims to provide a method for detecting the ratio of chiral inositol to inositol, which is simple and quick and has better accuracy. To achieve the above object, a first aspect of the present invention provides a method for detecting a ratio of chiral inositol to inositol, wherein the method comprises: Dissolving chiral inositol, inositol and water, and preparing at least 5 standard solution with different ratios of chiral inositol to inositol and same baume degree, wherein the value of baume degree of the standard solution is expressed as Be b‌; Measuring the optical rotation of each standard solution; ‌ drawing a standard curve by taking the ratio of chiral inositol to inositol as an abscissa and the optical rotation of the corresponding standard solution as an ordinate; Measuring the baume degree and the optical rotation of a sample solution, wherein the baume degree value of the sample solution is marked as Be Y‌, and the optical rotation value of the sample solution is marked as A; Substituting the optical rotation of the sample solution into the standard curve when Be Y＝Beb‌ to calculate the ratio of chiral inositol to inositol; When Be Y≠Beb, calculating the optical rotation B of the sample solution when the Baume degree is Be b according to a formula I; Formula I; substituting optical rotation B into the standard curve to calculate the ratio of chiral inositol to inositol. Compared with the prior art, the method has the advantages that the optical rotation of the chiral inositol and the inositol in different proportions is measured, the mass ratio of the chiral inositol to the inositol in the standard solution is taken as the abscissa, the optical rotation of the corresponding standard solution is taken as the ordinate, a standard curve is drawn, and when the baume degree Be b of the sample solution is measured to Be the same as the baume degree Be b of the standard solution, the optical rotation of the sample solution is measured, and the ratio of the chiral inositol to the inositol in the sample solution can Be directly measured by substituting the standard curve. The detection method does not need to dilute and/or concentrate the sample solution, thereby improving the detection efficiency. When the Baume degree Be b of the sample solution is measured to Be different from the Baume degree Be b of the standard substance solution, the optical rotation B of the sample solution when the Baume degree is Be b is calculated through a formula I, and the ratio of chiral inositol to inositol in the sample solution can Be directly measured by substituting the optical rotation B into a standard curve. The method is simple and quick, saves detection time and has better accuracy. In addition, the detection method of the invention aims at the production liquid of D-chiral inositol generated by enzymatic catalysis of inositol, can save the detection cost of liquid phase, has small occupation area of conventional polarimeter equipment, low price and easy operation, is convenient for first-line production personnel to automatically detect, and reduces the production cost of chiral inositol. Further, the baume degree of the standard solution is 5-10%. In the invention, when the Baume degree of the standard substance solution meets the range, the measured optical rotation data is more accurate, which is beneficial to improving the accuracy of the detection method. Further, the content of the chiral inositol is 10-90% based on the total mass of the chiral inositol and inositol in the standard solution. Further, the b