CN-122017110-A - Method for detecting components of injection for preventing or treating vitamin or calcium deficiency of dairy cows

Abstract

The invention belongs to the technical field of gas chromatography detection, and relates to a gas chromatography method for simultaneously measuring the contents of ethanol and isopropyl myristate. The gas chromatograph is set under the following conditions that the temperature of a FID detector, a polyethylene glycol 20M capillary column, a sample inlet is 240-260 ℃, the temperature of the detector is 240-260 ℃, the carrier gas is nitrogen, the flow rate is 0.9-2.5mL/min, the sample injection amount is 1 mu L, the temperature of the column is programmed to rise, the initial temperature is 80-90 ℃, and the temperature is raised to 180-190 ℃ at the speed of 9-12 ℃/min for at least 10min. The method provided by the invention can realize simultaneous detection of ethanol and isopropyl myristate, and after two substances are dissolved by the N, N-dimethylformamide solvent, the detected spectrogram has no phenomena of peak overlapping, tailing and the like, and has the advantages of high separation efficiency, high selectivity, high sensitivity, high analysis speed and wide application range.

Inventors

• WANG HONGBO
• ZHANG JIANXIN
• BAO XIAOYUN
• LI HUIJUAN
• YAN XUE
• QI CHUNLI
• LIU LINGLING
• SHI HU
• ZHENG LEI

Assignees

• 济南深蓝动物保健品有限公司

Dates

Publication Date

20260512

Application Date

20260209

Claims (3)

1. 1. A method for detecting components of injection for preventing or treating cow vitamin or calcium deficiency is characterized by weighing ethanol and nutmeg standard substances, respectively diluting with organic solvent to obtain a series of ethanol and nutmeg solutions with known concentration, performing gas chromatographic analysis to obtain spectrograms of the ethanol and nutmeg standard substances with series of concentrations, respectively, fitting to obtain an ethanol standard curve by taking the ethanol concentration as an abscissa and the peak area as an ordinate; The sample to be detected is diluted by an organic solvent, then is detected under the detection condition set by gas chromatography to obtain a gas chromatography, and then the quantitative analysis is carried out on the gas chromatography, wherein the set condition of the gas chromatography is a FID detector, a polyethylene glycol 20M capillary column, the temperature of a sample inlet is 240-260 ℃, the temperature of the detector is 240-260 ℃, the carrier gas is nitrogen, the flow rate is 0.9-2.5mL/min, the sample injection amount is 1 mu L, the column temperature is programmed to rise, the initial temperature is 80-90 ℃, the initial temperature is raised to 180-190 ℃ at the speed of 9-12 ℃/min, and at least 10min is maintained.
2. 2. The method for detecting components of injection for preventing or treating vitamin or calcium deficiency of dairy cows according to claim 1, wherein the gas chromatography is set under the conditions of polyethylene glycol 20M capillary column, length of 30M, film thickness of 0.25 μm, sample inlet temperature of 250 ℃, detector temperature of 250 ℃, carrier gas of nitrogen, flow rate of 1.8mL/min, sample injection amount of 1 μL, temperature of column temperature programmed, initial temperature of 85 ℃ and temperature rise to 185 ℃ at a rate of 10 ℃/min for 10min.
3. 3. The method for detecting components of an injection for preventing or treating vitamin or calcium deficiency of dairy cows according to claim 1, wherein the organic solvent is N, N-dimethylformamide.

Description

Method for detecting components of injection for preventing or treating vitamin or calcium deficiency of dairy cows Technical Field The invention belongs to the technical field of gas chromatography detection, relates to a method for detecting components of injection for preventing or treating cow vitamin or calcium deficiency, and particularly relates to a gas chromatography method for determining the content of ethanol and isopropyl myristate in vitamin ADE injection comparison medicines. Background Ethanol and isopropyl myristate are solvents or cosolvents commonly used in injection, are often combined and applied in injection prescriptions, and in Chinese pharmacopoeia 2020 edition, it is recorded that the two substances can be detected by gas chromatography, but the detection conditions of the two substances are different. In actual test, two substances are required to be detected respectively, and the process is complicated. Therefore, a gas chromatography method capable of simultaneously detecting ethanol and isopropyl myristate is developed, which is favorable for quality control of injection and saves inspection time and labor cost. However, because the difference of boiling points and polarities of the ethanol and the isopropyl myristate is large, the solvent and the chromatographic column adopted in the separate detection of the two substances cannot be realized, and the method for simultaneously detecting the two substances is not reported at present. Disclosure of Invention The invention provides a novel method for detecting components of injection for preventing or treating milk cow vitamin or calcium deficiency aiming at the problems existing in the simultaneous detection of traditional ethanol and isopropyl myristate. In order to achieve the above purpose, the invention is realized by adopting the following technical scheme: An injection component detection method for preventing or treating cow vitamin or calcium deficiency comprises weighing ethanol and nutmeg standard, diluting with organic solvent to obtain a series of ethanol and nutmeg solutions with known concentration, performing gas chromatography analysis to obtain ethanol and nutmeg standard spectrograms with serial concentrations, taking ethanol concentration as abscissa, peak area as ordinate, fitting to obtain ethanol standard curve, taking nutmeg concentration as abscissa, peak area as ordinate, fitting to obtain nutmeg standard curve, diluting to obtain sample to be detected with organic solvent, detecting under detection conditions set by gas chromatography to obtain gas spectrograms, and quantitatively analyzing the spectrograms, wherein the gas chromatography is set under the conditions of FID detector, polyethylene glycol 20M capillary column, sample inlet temperature of 240-260 ℃, detector temperature of 240-260 ℃ and carrier gas of nitrogen, flow rate of 0.9-2.5mL/min, sample inlet amount of 1 μL, temperature of 80-90 ℃ and initial temperature of 80-90 ℃ at 9-12 ℃ to 180-190 ℃ at least 10 ℃. Preferably, the gas chromatograph is set under the conditions that a polyethylene glycol 20M capillary column is 30M in length, 0.25 mu M in film thickness, 250 ℃ in sample inlet temperature, 250 ℃ in detector temperature, 1.8mL/min in flow rate of carrier gas, 1 mu L in sample inlet amount, 85 ℃ in initial temperature and 185 ℃ in 10 ℃ rate, and the temperature is maintained for 10min. Preferably, the organic solvent is N, N-dimethylformamide. Compared with the prior art, the invention has the advantages and positive effects that: 1. The method provided by the invention can realize simultaneous detection of ethanol and isopropyl myristate, and after two substances are dissolved by the N, N-dimethylformamide solvent, the detected spectrogram has no phenomena of peak overlapping, tailing and the like, the accuracy is high, the detection is performed in the sample pretreatment process, and the detection time and the labor cost are effectively saved. 2. The method provided by the invention is suitable for measuring the content of ethanol and isopropyl myristate in the injection for dairy cow vitamin or calcium deficiency. The method has the advantages of high separation efficiency, high selectivity, high sensitivity, high analysis speed and wide application range. Drawings FIG. 1 is a graph of a linear fit of ethanol concentration to peak area. Fig. 2 is a plot of isopropyl myristate concentration versus peak area fit. FIG. 3 is a sample detection chromatogram. FIG. 4 is a sample detection chromatogram of comparative example 1. Detailed Description In order that the above objects, features and advantages of the application will be more clearly understood, a further description of the application will be provided with reference to specific examples. It should be noted that, without conflict, the embodiments of the present application and features in the embodiments may be combined with each other. In the following description, numerous s