CN-122017138-A - Comprehensive biomarker response Index (IBR) based photoaging micro-plastic composite toxicity detection and risk judgment method

CN122017138ACN 122017138 ACN122017138 ACN 122017138ACN-122017138-A

Abstract

The invention belongs to the technical field of toxicity detection and risk assessment of environmental pollutants, and particularly relates to a comprehensive biomarker response Index (IBR) based photoaging microplastic composite toxicity detection and risk judgment method, which comprises the following steps of S1, preparing photoaging microplastic; S2, biological exposure experiments, S3, multidimensional biological response detection, S4, comprehensive biomarker response Index (IBR) calculation, and S5, composite toxicity risk judgment. The method integrates multiple biological responses by introducing the IBR method to form a single comprehensive index, improves objectivity and comparability of toxicity judgment, and is applicable to environmental pollutants such as pesticides, flame retardants, medicines and the like. The method has the advantages of short experimental period, high sensitivity and visual result, can truly reflect the biological influence of the photo-aging microplastic under the coexistence condition of pollutants, and has good application and popularization values.

Inventors

HU GUOCHENG
DING PING
ZHOU HUI
LI XINTONG
ZHANG WEIZHENG
LI XIN
ZHANG LIJUAN
ZHANG JIAYI

Assignees

生态环境部华南环境科学研究所(生态环境部生态环境应急研究所)
广州市干部和人才健康管理中心(广州市人才研修院、广州市第十一人民医院、广州市公职人员心理健康服务中心)

Dates

Publication Date: 20260512
Application Date: 20260129

Claims (9)

1. The photoaging micro-plastic composite toxicity detection and risk determination method based on comprehensive biomarker response Index (IBR) is characterized by comprising the following steps: s1, preparing photo-aging microplastic: carrying out ultraviolet light aging treatment on the original micro plastic to obtain photo-aging micro plastic; s2, biological exposure experiment: Dividing zebra fish embryos or juvenile fish into at least four exposure groups, including a blank control group, a pollutant single exposure group, an original microplastic and pollutant composite exposure group and a photoaged microplastic and pollutant composite exposure group, and performing exposure culture; s3, multidimensional biological response detection: Detecting a plurality of biological indexes related to the neurotoxicity of each group of zebra fish after the exposure is finished; s4, calculating a comprehensive biomarker response Index (IBR): performing standardization processing on each biological index measured in the step S3, and calculating comprehensive biomarker response Index (IBR) of each exposure group based on the standardized value; S5, judging the composite toxicity risk: comparing the IBR values of the exposure groups, and judging the influence of the photo-aging micro-plastic on the toxicity of the pollutants according to the IBR values of the photo-aging micro-plastic and pollutant combined exposure groups, the single pollutant exposure groups and the relative sizes of the IBR values of the original micro-plastic and pollutant combined exposure groups.
2. The method according to claim 1, wherein the microplastic in step S1 is a polystyrene microplastic having a particle size of 1.0±0.05 μm; the ultraviolet aging treatment is carried out by continuously irradiating for 14 days under 50 ℃ and 50% wet condition under the ultraviolet light source with the wavelength of 254 nm, and the total irradiation intensity is 58 mW cm -2 .
3. The method of claim 1, wherein the contaminant in step S2 is a persistent organic contaminant; the exposure time of the zebra fish embryos lasted from 2 h days after fertilization to 5 days after fertilization.
4. The method of claim 1, wherein in step S3, the plurality of biological indicators comprises embryo tail spontaneous movement, juvenile fish behaviours, neurotransmitter content, neuron fluorescence imaging.
5. The method of claim 4, wherein the behavioral indicators comprise embryo autokinetic movement frequency and/or average juvenile fish swimming speed, and wherein the neurotransmitter content comprises acetylcholine (ACh), 5-hydroxytryptamine (5-HT), and gamma-aminobutyric acid (GABA).
6. The method according to claim 1, wherein in step S4, the normalization process uses a formula Is carried out, wherein, As the index value of the index value, Is the mean value of the two values, Is the standard deviation.
7. The method of claim 6, wherein in step S4, the comprehensive biomarker response Index (IBR) is calculated according to the formula Wherein Δsi is the difference or change in biological index relative to the control group after normalization of item i.
8. The method according to claim 1, wherein in step S5, the decision rule is: (1) Respectively calculating IBR values of the independent exposure group of the pollutants, the composite exposure group of the original microplastic and the pollutants and the composite exposure group of the photo-aged microplastic and the pollutants; (2) Comparing the IBR values of the light-aged microplastic and contaminant composite exposure group with the IBR values of the contaminant individual exposure group: when the former is significantly higher than the latter, judging that the photoaged microplastic has an amplifying effect on the toxicity of pollutants under the composite exposure condition; (3) Further comparing the IBR values of the photo-aged microplastic and contaminant composite exposure group with the IBR values of the original microplastic and contaminant composite exposure group: when the two are close or have no obvious difference, the influence of the photo-aging process on the micro-plastic composite toxicity is judged to be limited; (4) When the IBR value of the light aging micro-plastic and pollutant composite exposure group is lower than the IBR value of the original micro-plastic and pollutant composite exposure group or lower than the IBR value of the pollutant independent exposure group, judging that the light aging micro-plastic has protection or antagonism on the pollutant toxicity under the composite exposure condition; (5) And according to the judging result, classifying and outputting the toxic effect type of the photo-aging micro-plastic under the condition of pollutant composite exposure.
9. The method of claim 1, wherein the method is useful for ecotoxicity screening, environmental risk stratification assessment, and regulatory decision support of new or typical contaminants in an environment.

Description

Comprehensive biomarker response Index (IBR) based photoaging micro-plastic composite toxicity detection and risk judgment method Technical Field The invention belongs to the technical field of toxicity detection and risk assessment of environmental pollutants, and particularly relates to a light aging micro-plastic composite toxicity detection and risk judgment method based on a comprehensive biomarker response Index (IBR). Background Microplastic becomes an important new source of contaminants as the plastic accumulates and photo ages in the environment for a long period of time. The photoaging changes the surface structure and chemical composition of the microplastic, so that the microplastic has stronger pollutant adsorption and biological interaction capability. The polystyrene microplastic (PS-MPs) generates a large amount of oxidation functional groups (such as carbonyl and hydroxyl) after aging, so that the adsorption capacity is obviously enhanced. When coexisting with persistent organic pollutants (such as hexabromocyclododecane and HBCD), obvious composite toxic effects can occur, and potential threats are caused to the neural development and the behavioural functions of aquatic organisms. The current microplastic toxicity research and risk supervision are mainly focused on original microplastic, but a large number of microplastic in the actual environment undergo aging processes such as illumination, oxidation and the like, the surface structure, the electrical property and the active components of the microplastic are obviously changed, the toxicity is possibly obviously enhanced, and the composite toxicity enhancement effect with environmental pollutants is generated. The existing detection method cannot quantify toxicity change caused by photo-aging by ① system, ② judges the amplifying effect of photo-aging microplastic on pollutant toxicity, ③ provides objective and quantitative composite toxicity judgment index, and ④ is used for new pollutant supervision and ecological safety rapid evaluation. In the prior art, the toxicity of the microplastic depends on a single index (such as swimming speed or ROS level), and a system integration and risk classification means are lacked, so that the compound pollution effect is difficult to objectively characterize. Therefore, it is highly desirable to establish a comprehensive judgment method based on multidimensional biological response signals to quantitatively reveal the degree and risk level of the composite toxicity of the photoaged microplastic. Disclosure of Invention The invention aims at solving the existing problems and provides a comprehensive biomarker response Index (IBR) based photoaging micro-plastic composite toxicity detection and risk judgment method. The invention is realized by the following technical scheme: A light aging micro plastic composite toxicity detection and risk determination method based on comprehensive biomarker response Index (IBR) comprises the following steps: s1, preparing photo-aging microplastic: carrying out ultraviolet light aging treatment on the original micro plastic to obtain photo-aging micro plastic; s2, biological exposure experiment: Dividing zebra fish embryos or juvenile fish into at least four exposure groups, including a blank control group, a pollutant single exposure group, an original microplastic and pollutant composite exposure group and a photoaged microplastic and pollutant composite exposure group, and performing exposure culture; s3, multidimensional biological response detection: Detecting a plurality of biological indexes related to the neurotoxicity of each group of zebra fish after the exposure is finished; s4, calculating a comprehensive biomarker response Index (IBR): performing standardization processing on each biological index measured in the step S3, and calculating comprehensive biomarker response Index (IBR) of each exposure group based on the standardized value; S5, judging the composite toxicity risk: comparing the IBR values of the exposure groups, and judging the influence of the photo-aging micro-plastic on the toxicity of the pollutants according to the IBR values of the photo-aging micro-plastic and pollutant combined exposure groups, the single pollutant exposure groups and the relative sizes of the IBR values of the original micro-plastic and pollutant combined exposure groups. Further, the microplastic in the step S1 is polystyrene microplastic with the particle size of 1.0+/-0.05 mu m; the ultraviolet aging treatment is carried out by continuously irradiating for 14 days under 50 ℃ and 50% wet condition under the ultraviolet light source with the wavelength of 254 nm, and the total irradiation intensity is 58 mW cm -2. Further, the contaminants in step S2 are persistent organic contaminants; the exposure time of the zebra fish embryos lasted from 2 h days after fertilization to 5 days after fertilization. Further, the plurality of biological indicators includes embryo