CN-122017142-A - Evaluation method and application of mesosulfuron-methyl herbicide-resistant wheat

Abstract

The invention discloses an evaluation method and application of mesosulfuron-methyl herbicide-resistant wheat, and belongs to the technical field of wheat breeding. The method comprises the following steps of detecting endogenous hormone content and specific metabolite content of wheat to be evaluated, detecting plant height, flag leaf shape, seed yield and quality index of the wheat, comparing the detection result with a non-applied control or a known variety, and comprehensively evaluating the tolerance of the wheat to the mesosulfuron. The invention constructs a set of multi-index comprehensive evaluation system. The method effectively overcomes the defects that the traditional method depends on subjective field observation and evaluation lag and has single index, realizes objective identification of the mesosulfuron-methyl resistance of the wheat variety, and provides reliable technical support for rapid screening of resistant varieties, early evaluation of breeding materials and field safety medication. The invention also provides application of the method in preparation of a detection kit.

Inventors

• CAO QIAO
• HE MINGQI
• GAO ZHENXIAN
• FU XIAOYI
• HAN RAN
• SHI ZHANLIANG
• BAN JINFU
• SHAN ZILONG
• GAO XINMEI

Assignees

• 石家庄市农林科学研究院

Dates

Publication Date

20260512

Application Date

20260211

Claims (9)

1. 1. The evaluation method of the mesosulfuron-methyl herbicide-resistant wheat is characterized by comprising the following steps of: s1, stress treatment, namely applying mesosulfuron-methyl to the wheat to be evaluated in the green-turning period; s2, physical and chemical index detection, namely carrying out endogenous hormone content detection and metabolite content detection on 10-15 days after application of the mesosulfuron-methyl; s3, detecting a phenotype index, namely, 18-24 days after the application of the mesosulfuron-methyl, and detecting the phenotype index; s4, detecting yield quality indexes, namely harvesting the wheat to be evaluated, and detecting the yield quality indexes; s5, comprehensively evaluating the tolerance of the wheat to be evaluated to the mesosulfuron-methyl herbicide by comparing the detection results of the wheat to be evaluated with the wheat of a control group without application or the wheat of the known mesosulfuron-methyl herbicide; Wherein the endogenous hormones include gibberellin, indoleacetic acid, and abscisic acid; the metabolites include creatine phosphate disodium salt and cystine.
2. 2. The method for evaluating wheat resistant to mesosulfuron-methyl herbicide according to claim 1, wherein in step S5, wheat to be evaluated having smaller gibberellin content decrease and smaller indoleacetic acid and abscisic acid content increase is evaluated as the wheat resistant to mesosulfuron-methyl herbicide, compared to a sensitive variety or a control group to which no pesticide is applied.
3. 3. The method for evaluating wheat resistant to mesosulfuron-methyl herbicide according to claim 2, wherein in step S5, wheat to be evaluated having smaller increase in creatine phosphate disodium salt content and smaller decrease in cystine content is evaluated as wheat resistant to mesosulfuron-methyl herbicide compared to a sensitive variety or a control group to which no drug is applied.
4. 4. A method of evaluating a wheat resistant to mesosulfuron-methyl herbicide as defined in claim 3, wherein said phenotypic indicator comprises wheat plant height and flag leaf morphology; the yield and quality indexes are the wheat grain yield, thousand grain weight, spike grain number, spike number and volume weight.
5. 5. The method for evaluating wheat resistant to mesosulfuron-methyl herbicide according to claim 4, wherein the method for detecting the plant height of wheat is to detect the length from the wheat floor to the top end of the main spike; The detection indexes of the flag leaf shape comprise the area, perimeter, length and width of the flag leaf.
6. 6. The method for evaluating a wheat resistant to a mesosulfuron-methyl herbicide according to any one of claims 1 to 5, characterized in that analysis of variance is performed on the results of detection of physicochemical index, phenotypic index and yield quality index to compare the level of difference significance.
7. 7. The method for evaluating wheat resistant to mesosulfuron-methyl herbicide as claimed in claim 6, wherein the wheat is harvested in a mature period and is harvested in real time for detecting a yield quality index.
8. 8. Use of the evaluation method of the mesosulfuron-methyl herbicide resistant wheat according to claim 7 for preparing a kit for detecting the tolerance of mesosulfuron-methyl Long Nai.
9. 9. A test kit for evaluating wheat's tolerance to a mesosulfuron-methyl herbicide, said kit comprising reagents for specifically detecting the content of endogenous hormones and metabolites in a wheat sample: The endogenous hormones are gibberellin, indoleacetic acid and abscisic acid; the metabolites are creatine phosphate disodium salt and cystine; the kit adopts the evaluation method of claim 2 or 3 as a criterion for tolerance to the mesosulfuron-methyl herbicide.

Description

Evaluation method and application of mesosulfuron-methyl herbicide-resistant wheat Technical Field The invention belongs to the technical field of wheat breeding, and particularly relates to an evaluation method of mesosulfuron-methyl herbicide-resistant wheat and application thereof. Background Wheat is one of the most important grain crops, and its production level is directly related to grain safety. Weeds in the wheat field not only compete with wheat for growth resources such as water, fertilizer and illumination, but also serve as a bridge for diseases and insect pests, and seriously jeopardize the yield and quality of the wheat. Although chemical weeding is the most important prevention and treatment means in wheat production due to the advantages of high efficiency, time saving, labor saving and the like, the problem of phytotoxicity is increasingly prominent along with the large and continuous use of herbicides, and the phytotoxicity cannot be ignored. Among many chemical herbicides, the sulfonylurea systemic herbicide mesosulfuron (Mesosulfuron-methyl) exerts a herbicidal action by inhibiting the activity of acetolactate synthase, and has remarkable control effects on various malignant gramineous weeds in wheat fields such as festival wheat, alopecuroide, brome and the like. However, the agent has different degrees of phytotoxicity risks on different wheat varieties, tolerance differences among varieties and influence of environmental stress, and can cause growth inhibition, leaf malformation and even yield reduction of sensitive varieties. Currently, methods for evaluating wheat tolerance to mesosulfuron-methyl herbicides rely primarily on field symptom observations or final yield determinations. The traditional methods have obvious defects that objective and quantitative evaluation is difficult to realize in field symptom observation, experience levels of observers are different, subjective judgment standards are different, and small changes with high identification difficulty cannot be captured by naked eye observation. If the tolerance of wheat to the mesosulfuron herbicide is judged only by relying on morphological indexes and yield, the result is inaccurate and the measurement and statistics workload is large, secondly, the existing evaluation system focuses on visible morphological indexes, ignores the endogenous physiological index change of plants in early stage of mesosulfuron stress, and still lacks a comprehensive evaluation method capable of integrating multidimensional and multi-period indexes. Disclosure of Invention In order to overcome the defects in the prior art, the invention firstly discloses and applies the correlation between the content change of specific metabolites and endogenous hormones gibberellin, indoleacetic acid and abscisic acid and the methyl disulfone tolerance performance of wheat, takes the correlation as one of early and sensitive evaluation indexes, combines with detection indexes such as physiological phenotype and the like, and aims to provide an early and systematic comprehensive evaluation method for the methyl disulfone herbicide-resistant wheat. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: the evaluation method of the mesosulfuron-methyl herbicide-resistant wheat comprises the following steps in sequence: s1, stress treatment, namely applying mesosulfuron-methyl to the wheat to be evaluated in the green-turning period; s2, physical and chemical index detection, namely carrying out endogenous hormone content detection and metabolite content detection on 10-15 days after application of the mesosulfuron-methyl; s3, detecting phenotype indexes, namely detecting the phenotype indexes 18 to 24 days after application; s4, detecting yield quality indexes, namely harvesting the wheat to be evaluated, and detecting the yield quality indexes; s5, comprehensively evaluating the tolerance of the wheat to be evaluated to the mesosulfuron-methyl herbicide by comparing the detection results of the wheat to be evaluated with the wheat of a control group without application or the wheat of the known mesosulfuron-methyl herbicide; Wherein the endogenous hormones include gibberellin, indoleacetic acid, and abscisic acid; the metabolites include creatine phosphate disodium salt and cystine. The inventor team unexpectedly discovers the association relation between endogenous hormones gibberellin, indoleacetic acid and abscisic acid, and the content change of metabolic products creatine phosphate disodium salt and cystine and the wheat methylsulfonyl Long Nai receptor, and provides clues for early diagnosis of the phytotoxicity of the methylsulfonyl weedicide. Preferably, the physicochemical index test is performed on day 14 after administration of mesosulfuron; phenotypic index assays were performed on day 21 after administration of mesosulfuron; Wherein, the 30g/L mesosulfuron-methyl dispersible oil suspending agent is