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CN-122017145-A - Method and system for analyzing nanomaterial spatial distribution effect in nanomaterial-plant interaction mechanism based on microfluidic chip

CN122017145ACN 122017145 ACN122017145 ACN 122017145ACN-122017145-A

Abstract

The invention belongs to the technical field of nanomaterial evaluation, and provides a method and a system for analyzing a nanomaterial spatial distribution effect in a nanomaterial-plant interaction mechanism based on a microfluidic chip. According to the method, plant roots are loaded in a middle cavity of a microfluidic chip (comprising at least 3 cavities which are mutually communicated) for plant adaptation, liquid culture mediums containing nano materials are respectively poured into cavities on two sides of the middle cavity, a plurality of space effect models are formed, each space effect model comprises a symmetrical exposure model (the concentration of the nano materials in the liquid culture mediums poured into the cavities on two sides is the same) and an asymmetrical exposure model (the concentration of the nano materials in the liquid culture mediums poured into the cavities on two sides is different), the total concentration of the nano materials in the liquid culture mediums poured into the cavities on two sides of the symmetrical exposure model and the asymmetrical exposure model is the same, and root growth process data are monitored and analyzed to obtain the plant root system. The invention can accurately analyze the space distribution effect of the nano material in heterogeneous environment.

Inventors

  • CHEN BAOLIANG
  • LAN YILUN
  • ZHU XIANGYU

Assignees

  • 浙江大学

Dates

Publication Date
20260512
Application Date
20260212

Claims (10)

  1. 1. The method for analyzing the nanomaterial space distribution effect in the nanomaterial-plant interaction mechanism based on the microfluidic chip is characterized in that the microfluidic chip comprises at least 3 cavities which are communicated with each other, and comprises the following steps: Filling liquid culture mediums containing nano materials into cavities at two sides of the cavity with the plant root system respectively to obtain a plurality of space effect models, wherein the space effect models comprise symmetrical exposure models and asymmetrical exposure models, the symmetrical exposure models are different in concentration of the nano materials in the liquid culture mediums filled into the cavities at two sides, the symmetrical exposure models are the same in concentration of the nano materials in the liquid culture mediums filled into the cavities at two sides, and the total concentration of the nano materials in the liquid culture mediums filled into the cavities at two sides of the symmetrical exposure models is the same; Monitoring data of plant root growth processes in a plurality of space effect models to obtain a database; and analyzing the database to obtain the spatial distribution effect of the nano material in the nano material-plant interaction mechanism.
  2. 2. The method according to claim 1, wherein a guiding protrusion is arranged in the middle cavity of the 3 cavities, the cross section of the guiding protrusion is triangular, and the guiding protrusion guides the root system of the plant to grow along the straight line of the cavity.
  3. 3. The method according to claim 1, wherein the 3 cavities are arranged in parallel, and two side cavities of the 3 cavities are symmetrically distributed on two sides of the middle cavity.
  4. 4. The method of claim 1, wherein the plant comprises arabidopsis thaliana.
  5. 5. The method of claim 1, wherein the nanomaterial comprises molybdenum disulfide nanoplatelets.
  6. 6. The method of claim 1 or 5, wherein the concentration of nanomaterial in the liquid medium is independently 0-200 mg/L.
  7. 7. The method of claim 1, wherein the stem of the plant is vertical to the horizontal during adaptation and plant root growth.
  8. 8. The method of claim 1, wherein the perfusion flow rate of the liquid medium is 0.2-1.0 ml/h.
  9. 9. The method of claim 1, wherein the root growth process data comprises microscopic images, active oxygen, auxin, ferrous ions, and fluorescent images.
  10. 10. A system for analyzing the effect of spatial distribution of nanomaterials in a nanomaterial-plant interaction mechanism based on microfluidic chips, comprising: A microfluidic chip; A growth process monitoring system; and a processing module for monitoring the data.

Description

Method and system for analyzing nanomaterial spatial distribution effect in nanomaterial-plant interaction mechanism based on microfluidic chip Technical Field The invention relates to the technical field of fertilizer evaluation, in particular to a method and a system for analyzing a nanomaterial spatial distribution effect in a nanomaterial-plant interaction mechanism based on a microfluidic chip. Background Nanomaterials (NMs) are widely used as new fertilizers or biostimulants in sustainable agriculture, but their environmental effects and biosafety assessment are challenging. The traditional research adopts a method of homogeneously mixing NMs into soil or culture medium, and the dose-dependent effect is examined. However, the real soil environment has a high degree of spatial heterogeneity, resulting in a non-uniform distribution of NMs across the rhizosphere, creating localized "hot spots". Therefore, the biological effect of NMs is the result of the combined action of the dose effect and the spatial distribution effect, and the traditional method can not separate the dose effect from the spatial distribution effect, so that the deep understanding of the NMs-plant interaction mechanism and the guidance of precise agricultural application are severely restricted. In recent years, the microfluidic technology provides a powerful tool for life science research by high-precision control and real-time in-situ observation capability of the microfluidic technology on a microenvironment. In the prior art, although a microfluidic chip is used for the research of interaction between plant root systems and microorganisms or nutrition gradient response, no report on how to independently regulate and control plant root system response by using a system to actively control and analyze NMs spatial distribution effect, which is a key environment variable, is yet to be seen. Therefore, a method capable of accurately simulating NMs spatial heterogeneity distribution and analyzing unique biological effects and mechanisms thereof in real time and in situ is developed and is important for promoting basic research and safety application of environmental nanotechnology. Disclosure of Invention In view of the above, the present invention is directed to a method and a system for analyzing the spatial distribution effect of nanomaterials in a nanomaterial-plant interaction mechanism based on a microfluidic chip. The method can accurately analyze the space distribution effect of the nano material in heterogeneous environment. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides a method for analyzing a nanomaterial spatial distribution effect in a nanomaterial-plant interaction mechanism based on a microfluidic chip, wherein the microfluidic chip comprises at least 3 cavities which are communicated with each other, and the method comprises the following steps of: Filling liquid culture mediums containing nano materials into cavities at two sides of the cavity with the plant root system respectively to obtain a plurality of space effect models, wherein the space effect models comprise symmetrical exposure models and asymmetrical exposure models, the symmetrical exposure models are different in concentration of the nano materials in the liquid culture mediums filled into the cavities at two sides, the symmetrical exposure models are the same in concentration of the nano materials in the liquid culture mediums filled into the cavities at two sides, and the total concentration of the nano materials in the liquid culture mediums filled into the cavities at two sides of the symmetrical exposure models is the same; Monitoring data of plant root growth processes in a plurality of space effect models to obtain a database; and analyzing the database to obtain the spatial distribution effect of the nano material in the nano material-plant interaction mechanism. Preferably, a guide protrusion is arranged in a middle cavity of the 3 cavities, the cross section of the guide protrusion is triangular, and the guide protrusion guides root systems of plants to grow along the straight line of the cavities. Preferably, the 3 cavities are arranged in parallel, and cavities on two sides of the 3 cavities are symmetrically distributed on two sides of the middle cavity. Preferably, the plant comprises arabidopsis thaliana. Preferably, the nanomaterial comprises molybdenum disulfide nanoplatelets. Preferably, the concentration of the nano material in the liquid culture medium is independently 0-200 mg/L. Preferably, the stems of the plants are vertical to the horizontal plane during adaptation of the plants and growth of the plant roots. Preferably, the perfusion flow rate of the liquid culture medium is 0.2-1.0 mL/h. Preferably, the root growth process data includes microscopic images, active oxygen, auxin, ferrous ions, and fluorescent images. The invention provides a system fo