CN-122017187-A - Method for detecting germination capacity of compound-induced broomrape seeds in soil

CN122017187ACN 122017187 ACN122017187 ACN 122017187ACN-122017187-A

Abstract

The application relates to a method for detecting compound induction broomrape seed germination capacity in soil, which is characterized in that broomrape seeds are buried under the soil through a containing bag, artificial compound medicines to be detected are applied in the soil, the application times are determined according to the needs, the water holding capacity of the soil is kept at 65% -70%, the containing bag is taken out after the 1 st application starts on the N th day, or the broomrape seeds are buried under the root soil of crops to be detected, cheng Zhuangdai is taken out after the N th day, and the capability of the applied medicines or rhizosphere secretions to stimulate the broomrape seeds is analyzed according to the broomrape seed germination capacity. The detection method can take out the broomrape seeds in the soil at any time and observe the germination condition of the broomrape seeds to infer whether the compound in the soil can stimulate the broomrape seeds to germinate, the detection process is time-saving and labor-saving, the detection cost is lower, and the detection result is more accurate, so the detection method has more popularization and application prospects.

Inventors

CHEN LIANFANG
LU MIN
WANG YINGSHAN
YANG HANLI
WANG JIANWEN
WANG YANPEI
FENG CHUANQI

Assignees

新疆生产建设兵团第二师农业科学研究所

Dates

Publication Date: 20260512
Application Date: 20251016

Claims (9)

1. A method for detecting the germination capacity of compound-induced broomrape seeds in soil, comprising the steps of: s1, preparing a detection device, namely preparing a net bottom dish for collecting broomrape seeds by using gauze to prepare a cavity containing bag with one end open; S2 detection of the stimulating ability of artificial compounds to germinate broomrape seeds: Placing at least 50 broomrape seeds in a field, fastening a bag opening, shaking the bag to scatter the broomrape seeds, paving the bag to be buried at a position 10 cm-15 cm below the soil, applying artificial compound medicines to be detected in the soil, determining the application times according to the needs, keeping the water holding capacity of the soil at 65% -70%, taking out Cheng Zhuangdai days from the 1 st application (N is a natural number larger than 1), flushing out external soil under flowing water, flushing the broomrape seeds into a net bottom dish, placing the net bottom dish under a magnifying glass of more than 15 times, and analyzing the capability of the applied medicines for stimulating the broomrape seeds according to the germination rate of the broomrape seeds; s3, detection of germination stimulation capability of crop rhizosphere secretion on broomrape seeds: placing at least 50 broomrape seeds into the containing bag, fastening the bag mouth, shaking the containing bag to disperse the broomrape seeds, spreading Cheng Zhuangdai to be buried at the position 10 cm-15 cm below the root soil of the crop to be detected in the field, taking out Cheng Zhuangdai after N (N is a natural number greater than 1) is used for days, flushing outside soil under running water, flushing the broomrape seeds into a net bottom dish, placing the net bottom dish under a magnifying glass which is more than 15 times, and analyzing the capability of stimulating the broomrape seeds by rhizosphere secretion according to the germination rate of the broomrape seeds.
2. The method for detecting the germination capacity of broomrape seeds induced by a compound in soil according to claim 1, wherein in S1, the holding bag is made of white nylon filter gauze with the aperture of 0.14mm, the filter gauze encloses a cavity with the side length of 3cm multiplied by 1.5cm multiplied by 1cm, wherein the bag mouth is one of 1cm multiplied by 1.5cm surfaces, and the other 1cm multiplied by 1.5cm surface is the bottom surface.
3. The method for detecting the germination capacity of broomrape seeds induced by a compound in soil according to claim 2, wherein the ring pocket is fixedly provided with an elastic ring, the elastic ring is a circular high-elasticity elastic ring with a cross section diameter of 2mm and a diameter of the ring in a natural state of 2cm.
4. The method for detecting the germination capacity of compound-induced broomrape seeds in soil according to claim 1, wherein the bottom dish comprises an annular ring, the inner diameter of the annular ring is 2.5cm, the outer diameter of the annular ring is 3cm, the wall thickness of the annular ring is 5mm, nylon gauze with the aperture of 0.14mm is arranged at the lower port of the annular ring, the nylon gauze shields the lower port of the annular ring, and the upper end of the annular ring is open.
5. A method for detecting the germination capacity of broomrape seeds induced by a compound in soil according to claim 3, wherein in S2, the opening of the bag is tied up by an elastic ring, one end of the marking tape is tied to the opening, the bag is buried under the soil, the other end of the marking tape is exposed out of the soil surface, and a marking rod is inserted at the marking tape.
6. A method for detecting the germination capacity of broomrape seeds induced by a compound in soil according to claim 3, wherein in S3, the opening of the containing bag is fastened by an elastic ring, one end of the marking belt is tied to the opening of the bag, the other end of the marking belt is exposed out of the soil surface after the containing bag is buried under the soil, and a marking rod is inserted at the marking belt, wherein the marking rod exceeds the top of the crop by 20cm.
7. The method for detecting the germination capacity of compound-induced broomrape seeds in soil according to claim 1, wherein in S3, the broomrape seeds in the field soil of the host crop are detected in the germination peak period, after the seedlings emerge from the host crop or after the transplanting, a plurality of holding bags are buried in the soil, the detection is carried out every 3 days, and when the germination rate of the extracted broomrape seeds reaches the highest peak, the optimal time for the application is reached.
8. The method for detecting the germination capacity of broomrape seeds induced by a compound in soil according to claim 1, wherein in S3, broomrape variety crops are screened, the host crops to be detected are detected once a month or once in the whole growth period, and the lower the germination rate of the broomrape seeds is, the more the broomrape variety crops can be made into broomrape crops.
9. The method for detecting the germination capacity of broomrape seeds induced by a compound in soil according to claim 1, wherein in S3, the broomrape seeds are screened for trapping crops, the non-host crops to be detected are detected once a month or once in the whole growth period, and the broomrape seeds can become trapped crops as the germination rate is higher.

Description

Method for detecting germination capacity of compound-induced broomrape seeds in soil Technical Field The application relates to the technical field of prevention and control of parasitic weed broomrape, in particular to a method for detecting the germination capacity of compound-induced broomrape seeds in soil. Background The broomrape is a parasitic weed, has no roots or green leaves, cannot absorb nutrition in soil, cannot carry out photosynthesis to produce nutrition, and can produce a sucker to penetrate through the root epidermis of a host plant after seed germination, absorb the nutrition and moisture of the host plant to grow and develop, so that the host is damaged, and the host crops of the broomrape are crops such as tomatoes, melons, stevia rebaudiana, sunflowers and the like, so that the yield and the quality of the crops are reduced after damage, the crops are reduced, and are in dead reception when serious, so that the income of farmers is reduced, and related processing industries are also reaped. The broomrape has the characteristics that 1, broomrape has strong fertility, each plant of broomrape can produce tens of thousands of seeds, so that a large number of offspring can be produced once a small amount of seed occurs, 2, the broomrape seeds have wide propagation paths and can be propagated through people and livestock moving everywhere, farm machinery and wind power water flow, and 3, the broomrape seeds have strong vitality and can survive for ten years in soil. Thus, as the years of planting of host crops increases, the broomrape hazard rapidly spreads and aggravates. The traditional broomrape control method is manual pulling, but the method is time-consuming and labor-consuming and is easy to damage the root of the host crops. The broomrape seeds are tiny like dust, mostly elliptical, about 0.3mm long and 0.15mm in diameter, the thousand grain weight is only 13mg, and the broomrape seeds cannot be identified after being scattered into the soil, so that the accurate germination time in the soil is not observed. Unlike common non-parasitic weeds, broomrape seeds need to germinate in soil under the stimulation of compounds such as strigolactone secreted by host roots, but the compounds such as strigolactone are difficult to extract and have poor stability. Based on this principle, researchers have proposed measures to mitigate the brookfield hazard from the following aspects: 1. Artificially synthesizing strigolactone analogues, applying the strigolactone analogues to soil when no host crops are planted, wherein the strigolactone analogues are expected to induce the emergence of broomrape seeds, the broomrape seeds die because no nutrition is found in the hosts after the emergence, the emergence becomes suicide emergence, the suicide emergence can reduce the live broomrape seeds in the soil, and the broomrape harm is greatly reduced after the host crops are planted in the following stubbles; 2. Screening and trapping crops, wherein some crop roots can secrete compounds to stimulate broomrape seeds to germinate in soil, but the broomrape seeds are not parasitic, and the broomrape seeds die after the broomrape seeds germinate because hosts cannot be found and nutrition is lacking; 3. Screening host crops of resistant broomrape or resistant broomrape varieties, wherein compounds secreted by the roots of the host crops of different varieties stimulate broomrape seeds to have strong or weak germination capacity, the host varieties with relatively weak stimulation capacity are called host crops of resistant broomrape varieties, and the host crops of resistant broomrape or resistant broomrape varieties are planted, so that the damage of broomrape can be reduced; 4. The medicament prevents and removes the broomrape seeds in the germination peak period. The secretion of the root of the host crop stimulates the broomrape seeds to germinate, the seed germination period is most sensitive to herbicide, and the weeding effect is optimal after the broomrape seeds germinate and before parasitizing. The damage to broomrape is reduced by applying herbicide based on the difference in sensitivity of broomrape to part of the herbicide as compared to the host. According to the measures for reducing the damage of the broomrape, the capability of the compound in the soil to stimulate the germination of the broomrape seeds needs to be detected, and the detection method in the prior art comprises the detection of the germination test in an indoor culture dish, the detection of a pot plant test and the detection of the peak period of the broomrape germination stimulated by the host crop by the yield of the broomrape in the field. The defect of the test of sprouting in the culture dish is that the broomrape seeds in the culture dish are in the air after absorbing water, the concentration, the duration and the like of the stimulus required by sprouting are quite different from those in the culture dish under the co