CN-122017197-A - Accurate quantitative analysis device for soil organic matter plants and microbial source carbon

CN122017197ACN 122017197 ACN122017197 ACN 122017197ACN-122017197-A

Abstract

The invention provides a precise quantitative analysis device for soil organic matter plants and microbial source carbon, and belongs to the technical field of soil environment analysis. The device comprises an analyzer main body, a rotating mechanism arranged at the bottom end of the detection groove and limiting mechanisms arranged at two sides of the detection groove. The rotating mechanism comprises a placing disc, a lower pressing ring, a driving rod, a transmission assembly and a rotating assembly, and automatic rotation of the detecting disc is realized through linkage of mechanical structures such as a ratchet wheel, a coil spring and friction transmission. The invention solves the technical problems of high labor intensity, low efficiency and poor uniformity of shaking effect in the prior art that the soil in the detection tray is subjected to shaking pretreatment by manpower. By automatically converting the 'putting in' action into centrifugal shaking, the efficiency and uniformity of sample pretreatment are obviously improved, and the accuracy and reliability of the follow-up quantitative analysis data of the carbon source are ensured.

Inventors

SUN JIALI
LI FEI
TIAN SHICHAO
YUN XIANGJUN
BAI HAIHUA
LIU XIAOLONG
CHUN LIANG
GUO XIAOMENG

Assignees

中国农业科学院草原研究所

Dates

Publication Date: 20260512
Application Date: 20260226

Claims (10)

1. Accurate quantitative analysis device of soil organic matter plant and microorganism source carbon, characterized by comprising: The analyzer comprises an analyzer main body (100), wherein the top end of the analyzer main body (100) is rotationally connected with a sealing cover (101), the upper surface of the analyzer main body (100) is provided with a detection groove (102), and a detection disc (103) is arranged in the detection groove (102); The rotary mechanism (200) is arranged at the bottom end of the detection groove (102), the rotary mechanism (200) comprises a placing disc (201) which is arranged in the detection groove (102) in a suspending mode, a lower pressing ring (202) is fixedly connected to the bottom end of the placing disc (201), the bottom end of the lower pressing ring (202) extends to the inside of the analyzer main body (100), the outer side of the lower pressing ring (202) is supported with the inner wall of the analyzer main body (100) through an elastic component (203), driving rods (204) are symmetrically arranged at the left side and the right side of the inner wall of the lower pressing ring (202), a transmission component (205) is connected between the two driving rods (204) in a transmission mode, a rotary component (206) is connected to the inner side of the transmission component (205), the rotary component (206) comprises a central shaft (206 a) which is rotatably connected to the inner center of the analyzer main body (100), and the top end of the central shaft (206 a) extends to the bottom of the detection groove (102) and is in transmission connection with the detection disc (103) through a friction component (207); The device also comprises limiting mechanisms (300) fixedly arranged on the left side and the right side of the top end of the detection groove (102).
2. The device for accurately quantifying and analyzing the carbon of the soil organic matter plant and the microorganism source according to claim 1, wherein a first rotating cavity (104) is formed in the analyzer main body (100) and located right below the detection groove (102), a second rotating cavity (105) is communicated with the bottom end of the first rotating cavity (104), limit grooves (106) are formed in the left side and the right side of the first rotating cavity (104), and expansion grooves (107) are formed in the outer sides of the two limit grooves (106).
3. The device for precisely quantifying and analyzing the carbon of the soil organic matter plant and the microorganism source according to claim 2, wherein the bottom ends of the lower pressure ring (202) are telescopically connected to the inner side of the interior of the telescopic groove (107), the elastic component (203) comprises supporting plates (203 a) fixedly arranged on the left side and the right side of the outer surface of the lower pressure ring (202), the two supporting plates (203 a) are respectively and slidably connected to the outer sides of the two telescopic grooves (107), the bottom ends of the two supporting plates (203 a) are fixedly connected with telescopic springs (203 b), and the bottom ends of the two telescopic springs (203 b) are fixedly connected with the bottom wall of the telescopic groove (107).
4. The device for accurately quantifying and analyzing soil organic plant and microbial source carbon according to claim 3, wherein the transmission assembly (205) comprises a rotary table (205 a) rotatably connected to the inner top wall of the first rotary cavity (104), a hollow rotary ring (205 b) is fixedly connected to the bottom end of the rotary table (205 a), two spiral grooves (205 c) are circumferentially arrayed on the surface of the hollow rotary ring (205 b), and openings (205 d) are formed in the bottom ends of the two spiral grooves (205 c).
5. The device for accurately quantifying and analyzing the carbon content of the organic matters in soil and the microorganism source according to claim 4, wherein the opposite surfaces of the two driving rods (204) respectively penetrate through the two limiting grooves (106) and extend into the first rotating cavity (104), and the inner ends of the two driving rods (204) are respectively arranged under the two openings (205 d).
6. The device for accurately quantifying and analyzing the carbon of the soil organic matter plant and the microorganism source according to claim 5, wherein the rotating assembly (206) further comprises a rotating shaft (206 b) fixedly installed at the bottom end of the central shaft (206 a), the central shaft (206 a) is rotatably connected to the centers of the first rotating cavity (104) and the second rotating cavity (105) through the rotating shaft (206 b), the outer wall of the lower side of the central shaft (206 a) is fixedly connected with the initial end of a coil spring (206 c), and the tail end of the coil spring (206 c) is fixedly connected with the inner wall of the second rotating cavity (105).
7. The precise quantitative analysis device for soil organic matter plants and microbial source carbon according to claim 6, wherein the center of the turntable (205 a) is hollow, ratchet teeth (205 e) are arranged on the circumference array of the inner wall of the turntable, the upper portion of the central shaft (206 a) sequentially penetrates through the centers of the hollow swivel (205 b) and the turntable (205 a) and is coaxially arranged with the hollow swivel, storage grooves (206 a 1) are symmetrically formed in the left end and the right end of the outer surface of the upper side of the central shaft (206 a), pawls (206 d) matched with the ratchet teeth (205 e) are rotatably connected in the two storage grooves (206 a 1), the inner sides of the two pawls (206 d) are elastically and telescopically connected in the corresponding storage grooves (206 a 1) through bending springs (206 e), and the clamping ends of the two pawls (206 d) are spliced in the ratchet teeth (205 e) close to the corresponding pawls.
8. The device for accurately quantifying and analyzing the carbon of the soil organic matter plant and the microorganism source according to claim 7, wherein a connecting groove (103 a) is formed in the center of the bottom end of the detection disc (103), a connecting disc (206 f) matched with the connecting groove (103 a) is fixedly arranged at the top end of the central shaft (206 a), the friction assembly (207) comprises an inner friction disc (207 a) fixedly arranged in the connecting groove (103 a), an outer friction disc (207 b) fixedly arranged in the connecting disc (206 f), and the upper surface of the outer friction disc (207 b) is abutted against the bottom surface of the inner friction disc (207 a).
9. The device for accurately quantifying and analyzing soil organic plant and microbial source carbon according to claim 8, wherein a through groove (201 a) is formed in the center of the bottom of the placing plate (201), the inner diameter of the through groove (201 a) is larger than the outer diameter of the connecting groove (103 a), and the connecting disc (206 f) is coaxially arranged under the through groove (201 a).
10. The device for accurately quantifying and analyzing the carbon of the soil organic matter plant and the microorganism source according to claim 9, wherein the limiting mechanism (300) comprises fixed plates (301) fixedly arranged at the left side and the right side of the top end of the detection groove (102), sliding grooves (302) are respectively arranged on the opposite surfaces of the two fixed plates (301), limiting stops (303) are respectively and slidably connected in the two sliding grooves (302), return springs (304) are respectively and fixedly connected on the opposite surfaces of the two limiting stops (303), the other ends of the two return springs (304) are fixedly connected with the inner wall of the sliding grooves (302), and poking blocks (305) are respectively and fixedly arranged at the top ends of the two limiting stops (303); Guide inclined planes (303 a) are formed in the upper sides of the two limit stops (303), arc grooves (303 b) are formed in the bottom ends of the two limit stops (303), and the top edge of the detection disc (103) is slidably connected in the arc grooves (303 b) in the bottoms of the two limit stops (303).

Description

Accurate quantitative analysis device for soil organic matter plants and microbial source carbon Technical Field The invention relates to the technical field of soil environment analysis, in particular to a precise quantitative analysis device for soil organic matter plants and microbial source carbon. Background The accurate quantitative analysis device for the organic matter plant and microorganism source carbon has the core functions that organic carbon in a soil sample can be subjected to accurate tracing and quantification through an integrated physical, chemical or spectroscopic method (such as a detection module based on a biomarker or a stable isotope), and the content of carbon components derived from plant residues (such as lignin and cellulose derivatives) and carbon components derived from microorganisms and metabolites thereof (such as amino sugar and phospholipid fatty acid) is distinguished and accurately calculated, so that key data support is provided for evaluating the stability of a soil carbon reservoir, the function of a microorganism carbon pump and the health state of the soil. However, the existing accurate quantitative analysis device has a significant defect in the actual operation flow, which affects the analysis efficiency and the stability of the result. Specifically, after a soil sample to be detected is quantitatively loaded into a special detection disc, in order to ensure that an analyzer can obtain uniform and representative signals in subsequent detection, the soil in the detection disc is generally required to be sufficiently and uniformly shaken, and then the soil is stably placed into a detection groove of an analyzer body for reading, and the step is important for acquiring accurate and reliable data. However, when facing a large number of sample detection tasks, the repeated shaking and placing operation which is performed manually is not only greatly increased in labor intensity of workers, but also the shaking force, time length and uniformity are difficult to be kept consistent due to uncontrollable manual operation, so that the shaking effect of the subsequent samples is reduced. The inconsistent pretreatment can directly introduce human errors, influence the comparability of data among samples, and finally restrict the accuracy and the efficiency of high-throughput detection of the device. Disclosure of Invention This section is intended to outline some aspects of embodiments of the application and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the application and in the title of the application, which may not be used to limit the scope of the application. The present invention has been made in view of the above and/or problems occurring in the prior art. Therefore, the invention aims to solve the technical problem of providing an integrated analysis device capable of automatically, efficiently and uniformly finishing pretreatment (shaking) of soil samples so as to solve the problems of high labor intensity and poor consistency caused by manual operation, thereby ensuring the accuracy and reliability of data under high-throughput detection. In order to solve the technical problems, the invention provides the technical scheme that the accurate quantitative analysis device for soil organic matter plants and microbial source carbon comprises; the analyzer comprises an analyzer main body, wherein the top end of the analyzer main body is rotatably connected with a sealing cover, the upper surface of the analyzer main body is provided with a detection groove, and a detection disc is arranged in the detection groove; The rotary mechanism comprises a placing disc which is arranged in the detection groove in a suspending mode, a lower pressing ring is fixedly connected to the bottom end of the placing disc, the bottom end of the lower pressing ring extends to the inside of the analyzer main body, the outer side of the lower pressing ring is supported with the inner wall of the analyzer main body through an elastic component, driving rods are symmetrically arranged on the left side and the right side of the inner wall of the lower pressing ring, a transmission component is connected between the two driving rods in a transmission mode, the inner side of the transmission component is connected with the rotary component in a transmission mode, the rotary component comprises a central shaft which is connected to the center of the inside of the analyzer main body in a rotating mode, and the top end of the central shaft extends to the bottom of the detection groove and is connected with the detection disc in a transmission mode through a friction component; the device also comprises limiting mechanisms fixedly arranged at the left side and the right side of the top end of the detection groove. The precise quantitative analysis device for the soil organic matter plant and the microbia