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CN-122017217-A - One-card-three-way dry chemical detection reagent strip, detection system and method

CN122017217ACN 122017217 ACN122017217 ACN 122017217ACN-122017217-A

Abstract

The invention discloses a one-card-three-joint dry chemical detection reagent strip, a detection system and a detection method, and relates to the technical field of dry biochemical detection. The one-card triple-dry chemical detection reagent strip comprises a card body substrate and three reaction channels arranged on the card body substrate, wherein the three reaction channels are independent of each other and are respectively a glucose reaction channel, a uric acid reaction channel and a triglyceride reaction channel, each reaction channel comprises a sample receiving layer, a reagent reaction layer, a water absorption layer and an optical detection window, and the reagent reaction layer comprises an enzyme preparation, a substrate, a chromogenic reagent, EDTA or salts thereof. EDTA is arranged in the reagent reaction layer to complex and detect Ca 2+ in a sample, so that the coagulation and ion interference risk caused by a hypercalcemia sample is reduced.

Inventors

  • WU HONG
  • YUAN YAQI
  • MA YIRUI
  • SUN YUHAN

Assignees

  • 天津师范大学

Dates

Publication Date
20260512
Application Date
20260328

Claims (10)

  1. 1. A one-card three-way dry detection reagent strip is characterized by comprising a card body substrate and three reaction channels arranged on the card body substrate, wherein the three reaction channels are independent of each other and are a glucose reaction channel, a uric acid reaction channel and a triglyceride reaction channel respectively, each reaction channel comprises a sample receiving layer, a reagent reaction layer, a water absorption layer and an optical detection window, and the reagent reaction layer comprises an enzyme preparation, a substrate, a chromogenic reagent, EDTA or salts thereof.
  2. 2. The one-card three-way dry detection reagent strip of claim 1, wherein the glucose reaction channel adopts a double-enzyme coupling colorimetric method, and the reagent reaction layer comprises glucose dehydrogenase, nicotinamide adenine dinucleotide, diaphorase, thiazole blue, EDTA or salts thereof.
  3. 3. The one-card three-way dry detection reagent strip of claim 1, wherein the uric acid reaction channel adopts a uricase-peroxidase coupling method, and the reagent reaction layer comprises uricase, peroxidase, 3-methyl-2-benzothiazolinone hydrazone hydrochloride, dimethylaniline, EDTA or salts thereof.
  4. 4. The one-card three-way dry detection reagent strip of claim 1, wherein the triglyceride reaction channel adopts an LPL-GK coupling method, and the reagent reaction layer comprises lipoprotein lipase, glycerol kinase, glycerophosphate oxidase, peroxidase, 4-aminoantipyrine, N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt, EDTA or salts thereof.
  5. 5. The method for preparing the one-card-triplex dry chemical detection reagent strip according to any one of claims 1 to 4, which is characterized by comprising the following steps: (1) Injection molding a high polymer raw material to obtain a card body substrate, wherein the card body substrate comprises a sample adding area and three reaction channels; (2) Pretreating and cutting a sample receiving pad, a reaction membrane and a water absorbing pad; (3) Preparing three impregnating solutions of glucose impregnating solution, uric acid impregnating solution and triglyceride impregnating solution; (4) Respectively soaking the three reaction membranes in glucose soaking liquid, uric acid soaking liquid and triglyceride soaking liquid, and then draining and drying to respectively obtain a glucose reaction layer, a uric acid reaction layer and a triglyceride reaction layer; (5) The glucose reaction layer, the uric acid reaction layer and the triglyceride reaction layer are respectively stuck on three reaction channels of the card body substrate by adopting glue and are aligned with the optical detection windows, corresponding reagent reaction layers are respectively formed in the three reaction channels after the glucose reaction layer, the uric acid reaction layer and the triglyceride reaction layer are stuck, a sample receiving pad is stuck at the joint of a sample adding area and a shunt channel by adopting glue and is in close contact with the three reaction channels, a sample receiving layer is formed after the sample receiving pad is stuck, a water absorbing pad is stuck at the tail ends of the three reaction channels by adopting glue and is in continuous chromatographic detection, and a water absorbing layer is formed after the water absorbing pad is stuck; (6) And covering a transparent film on the surface of the card body substrate, performing hot pressing lamination, and then drying and packaging to obtain the one-card-triple chemical detection reagent strip.
  6. 6. The method for preparing a one-card three-way dry detection reagent strip according to claim 5, wherein the three impregnating solutions comprise EDTA or salts thereof in an amount of 2.0-8.0 mmol/L.
  7. 7. The application of the one-card three-way dry chemical detection reagent strip according to any one of claims 1 to 4, wherein the one-card three-way dry chemical detection reagent strip is applied to a detection process of glucose, uric acid or triglyceride in a serum sample or a whole blood sample of dogs.
  8. 8. A one-card three-way dry chemical detection system is characterized by comprising the one-card three-way dry chemical detection reagent strip, a reading device and a calibration information carrier, wherein the one-card three-way dry chemical detection reagent strip is disclosed in any one of claims 1-4, the reading device is used for respectively reading optical signals of a glucose reaction channel, a uric acid reaction channel and a triglyceride reaction channel, and the calibration information carrier is used for converting the optical signals into concentration values of corresponding items.
  9. 9. The one-card three-way dry chemical detection system according to claim 8, wherein the reading device is any one of a reflective colorimeter, a transmissive photometer, and an imaging reading device, or a combination thereof, and the calibration information carrier is any one of a batch ID card, a two-dimensional code, a barcode, and an electronic calibration file, or a combination thereof.
  10. 10. A method of detection using the detection system according to any one of claims 8 or 9, comprising the steps of: S1, adding a sample to be detected to a sample adding area of a one-card three-way dry chemical detection reagent strip, and enabling the sample to be detected to enter three reaction channels respectively; s2, completing enzymatic chromogenic reaction of three reaction channels in preset time; S3, the reading device reads colorimetric signals of the three reaction channels; And S4, calling batch calibration information to convert colorimetric signals of the three reaction channels into concentrations of glucose, uric acid and triglyceride respectively and outputting the concentrations.

Description

One-card-three-way dry chemical detection reagent strip, detection system and method Technical Field The invention relates to the technical field of dry biochemical detection, in particular to a one-card-triplex dry chemical detection reagent strip, a detection system and a detection method. Background Glucose, uric acid and triglyceride are common metabolic indexes in animal clinic, wherein glucose is used for screening and monitoring abnormal glucose metabolism, uric acid is used for evaluating related purine metabolism and renal excretion and can be used as related indexes for assisting diagnosis of hyperuricemia, and triglyceride is used for evaluating abnormal lipid metabolism. The existing laboratory detection often depends on a large-scale biochemical instrument, has long turnover time, and is unfavorable for the instant decision of primary diagnosis and treatment. The dry biochemical reagent strip has the advantages of simple operation, small sample consumption, adaptation to field detection and the like. Chinese patent CN118294442a discloses a saliva glucose test strip, which specifically comprises a detection reagent, a polymer film and filter paper, wherein the detection reagent comprises a saliva mucolytic agent and a detection drug, the detection drug is adhered to and infiltrated on the filter paper, the saliva mucolytic agent is adhered to and infiltrated on the polymer film, and the polymer film and the filter paper are combined into a whole to obtain the saliva glucose test strip. The test strip can detect saliva glucose, realize noninvasive and convenient monitoring of blood glucose level, and reduce the number of people who obtain accurate detection results through invasive detection when blood glucose is detected. Despite the advantages of using reagent strips for detection, there is still a need for a systematic solution in terms of multi-project integration, channel consistency, serum high calcium/clotting risk control, and long-term stability. Disclosure of Invention The invention aims to provide a one-card triple-dry chemical detection reagent strip, a detection system and a detection method, which are used for solving the problems that the existing chemical detection reagent strip cannot integrate various detection, and the high-calcium serum detection has higher coagulation risk, poor stability and the like. In order to achieve the above purpose, the first aspect of the present invention provides a card triple dry chemical detection reagent strip, which comprises a card body substrate and three reaction channels arranged on the card body substrate, wherein the three reaction channels are independent of each other and are a glucose reaction channel, a uric acid reaction channel and a triglyceride reaction channel respectively, each reaction channel comprises a sample receiving layer, a reagent reaction layer, a water absorbing layer and an optical detection window, and the reagent reaction layer comprises an enzyme preparation, a substrate, a chromogenic reagent, EDTA or salts thereof. Preferably, the glucose reaction channel adopts a double-enzyme coupling colorimetric method, and the reagent reaction layer comprises glucose dehydrogenase, nicotinamide adenine dinucleotide, diaphorase, thiazole blue, EDTA or salts thereof. Preferably, the uricase reaction channel adopts a uricase-peroxidase coupling method, and the reagent reaction layer comprises uricase, peroxidase, 3-methyl-2-benzothiazolinone hydrazone hydrochloride, dimethylaniline, EDTA or salts thereof. Preferably, the triglyceride reaction channel adopts an LPL-GK coupling method, and the reagent reaction layer comprises lipoprotein lipase, glycerol kinase, glycerophosphate oxidase, peroxidase, 4-aminoantipyrine, N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt, EDTA or salts thereof. The second aspect of the invention provides a method for preparing a one-card three-way dry chemical detection reagent strip, comprising the following steps: (1) Injection molding a high polymer raw material to obtain a card body substrate, wherein the card body substrate comprises a sample adding area and three reaction channels; (2) Pretreating and cutting a sample receiving pad, a reaction membrane and a water absorbing pad; (3) Preparing three impregnating solutions of glucose impregnating solution, uric acid impregnating solution and triglyceride impregnating solution; (4) Respectively soaking the three reaction membranes in glucose soaking liquid, uric acid soaking liquid and triglyceride soaking liquid, and then draining and drying to respectively obtain a glucose reaction layer, a uric acid reaction layer and a triglyceride reaction layer; (5) The glucose reaction layer, the uric acid reaction layer and the triglyceride reaction layer are respectively stuck on three reaction channels of the card body substrate by adopting glue and are aligned with the optical detection windows, corresponding reagent reaction layers are