Search

CN-122017223-A - Measurement module and chemiluminescence immunoassay analyzer

CN122017223ACN 122017223 ACN122017223 ACN 122017223ACN-122017223-A

Abstract

The invention discloses a measuring module and a chemiluminescent immunoassay analyzer, which belongs to the technical field of chemiluminescent immunoassay, and comprises a measuring part, wherein the measuring part comprises an analyzer main body, an injection assembly for injecting trace substances is arranged in the analyzer main body, a first chute and a second chute are arranged in the analyzer main body, a micro magnetic shielding sheet is arranged, a magnetic field is weakened firstly to prevent adsorption of magnetic beads from bumping walls, then weakening is relieved to gather the magnetic beads, finally weakening is carried out to enable the magnetic beads to be tiled again, dynamic adjustment is realized, the central gathering of 'no offset, no adherence and no edge accumulation' of the magnetic beads is realized through a symmetrical gradient weak magnetic field formed by a main magnetic block and an annular magnetic block, finally, a magnetic field interference dead zone is eliminated by virtue of a shielding sheet, the magnetic beads form a uniform tiling state, the conditions that the surface luminescence of the magnetic beads is blocked by each other and signal acquisition errors caused by the offset, the adherence or the edge accumulation of the magnetic beads are avoided, and the detection stability and accuracy are improved.

Inventors

  • WANG YANING
  • CHEN YILIN
  • DUAN HONGRU
  • Ji Beiying
  • YAN CHENGFU

Assignees

  • 深圳威高生基医疗科技有限公司

Dates

Publication Date
20260512
Application Date
20260320

Claims (10)

  1. 1. The measuring module and the chemiluminescent immunoassay analyzer are characterized by comprising The measuring component comprises an analyzer main body (10), an injection assembly (11) for injecting trace substances is arranged in the analyzer main body (10), a first sliding groove (12) and a second sliding groove (14) are arranged in the analyzer main body (10), and a measuring assembly (13) for detecting chemiluminescence signals in a reaction cup (106) is arranged in the analyzer main body (10); Tiling part, it is including setting up in the regulation subassembly (20) in analyzer main part (10) for adjust the magnetic bead in reaction cup (106), be provided with in regulation subassembly (20) and be used for weakening shielding assembly (21) of main magnetic path (205) magnetic field, be provided with in analyzer main part (10) and be used for carrying out transmission subassembly (22) of adjusting shielding assembly (21).
  2. 2. The measurement module and the chemiluminescent immunoassay analyzer of claim 1 wherein the analyzer body (10) comprises a body housing (101), a feed chute (102) is formed in the side surface of the body housing (101), a conveyor belt (103) is arranged in the feed chute (102), a plurality of clamping grooves (104) are formed in the conveyor belt (103), clamping blocks (105) are clamped in the clamping grooves (104), and reaction cups (106) are arranged on the side surface of the clamping blocks (105); The conveyer belt (103) runs through feed chute (102), the size of reaction cup (106) and the size looks adaptation of feed chute (102), reaction cup (106) are located the inside of feed chute (102), first spout (12) have been seted up to the inside of main part shell (101), first spout (12) are located the top of feed chute (102).
  3. 3. The measurement module and the chemiluminescent immunoassay analyzer of claim 2 wherein the injection assembly (11) comprises a motor (111) disposed at the top of the main body casing (101), a screw (112) is disposed below the motor (111), a slide plate (113) is screwed on the screw (112), and an injection head (114) is disposed at the bottom of the slide plate (113); The top that main part shell (101) is run through to lead screw (112) extends to the inside of first spout (12), injection head (114) are located the side of lead screw (112), sliding connection between the inner wall of lead screw (112) and first spout (12), the bottom of injection head (114) runs through the inner wall of first spout (12) and extends to the inside of feed chute (102), injection head (114) are located directly over reaction cup (106).
  4. 4. The measurement module and the chemiluminescent immunoassay analyzer of claim 3 wherein the measurement assembly (13) comprises a first connecting plate (131) fixedly connected to the side of the slide plate (113), a first rack (132) is fixedly connected to the bottom of the first connecting plate (131), a hollowed-out groove (133) is formed in the first rack (132), a driving gear (134) is meshed with the side of the first rack (132), a bevel gear (135) is fixedly connected to the side of the driving gear (134), a transmission gear (136) is arranged below the driving gear (134), a second rack (137) is meshed with the side of the transmission gear (136), a shade (138) is fixedly connected to the bottom of the second rack (137), and a photon counter (139) is arranged inside the shade (138).
  5. 5. The measurement module and the chemiluminescent immunoassay analyzer of claim 4 wherein the first connecting plate (131) is located at one side of the injection head (114) far away from the screw rod (112), the transmission gear (136) is located above the light shielding cover (138), a bevel gear (135) is fixedly connected to the top of the transmission gear (136), the two bevel gears (135) are meshed with each other, the first rack (132) is located at one side of the light shielding cover (138) far away from the injection head (114), the second rack (137) penetrates through the hollow groove (133), the second rack (137) is in sliding connection with the hollow groove (133), the first connecting plate (131) is in sliding connection with the first chute (12), the second chute (14) is arranged in the interior of the feed groove (102), the light shielding cover (138) is in sliding connection with the second chute (14), and the second rack (137) penetrates through the inner wall of the second chute (14) to extend to the outer side of the main body (101).
  6. 6. The measurement module and the chemiluminescent immunoassay analyzer of claim 2 wherein the adjustment assembly (20) comprises a cylinder (201) disposed inside a main body casing (101), wherein a telescopic rod (202) is disposed in the cylinder (201), an adjustment casing (203) is fixedly connected to the top of the telescopic rod (202), an annular magnet (204) and a main magnet (205) are disposed inside the adjustment casing (203), and an adjustment groove (206) is formed inside the main body casing (101); The main magnet (205) is located at the center of the annular magnet (204), the adjusting groove (206) is located below the feeding groove (102), the adjusting groove (206) is communicated with the feeding groove (102), and the adjusting shell (203) is slidably connected with the inner wall of the adjusting groove (206).
  7. 7. The measurement module and the chemiluminescent immunoassay analyzer of claim 6 wherein the shielding assembly (21) comprises two third sliding grooves (211) formed in the adjusting housing (203), wherein the third sliding grooves (211) are internally and slidably connected with second connecting plates (212), one sides of the two second connecting plates (212) close to each other are fixedly connected with micro-magnetic shielding sheets (213), a first elastic piece (214) and an air bag (215) are arranged in the third sliding grooves (211), ventilation grooves (216) are formed in the adjusting housing (203), and corrugated pipes (217) are arranged on two sides of the adjusting housing (203).
  8. 8. The measurement module and the chemiluminescent immunoassay analyzer of claim 7 wherein the two micro magnetic shielding plates (213) are combined to form a shielding plate, the cross section of the main magnetic block (205) is identical to the cross section of the shielding plate, the shielding plate is located right above the main magnetic block (205), the second connecting plate (212) is elastically connected with the inner wall of the third sliding groove (211) through a first elastic piece (214), the open end of the air bag (215) is fixedly connected with the inner wall of the third sliding groove (211), the air bag (215) is located on one side, far away from the micro magnetic shielding plate (213), of the second connecting plate (212), one side, close to the second connecting plate (212), of the air bag (215) is fixedly connected with the second connecting plate (212), the first elastic piece (214) is located between the micro magnetic shielding plate (213) and the air bag (215), the inside of the corrugated pipe (217) is communicated with the inside of the air bag (215) through a ventilation groove (216), and one end, far away from the groove (216), of the corrugated pipe (217) is fixedly connected with the inner wall of the regulation groove (206).
  9. 9. The measurement module and the chemiluminescent immunoassay analyzer of claim 7 wherein the transmission assembly (22) comprises a pressing plate (221) fixedly connected to one side of the sliding plate (113) far away from the first connecting plate (131), and two cavities (224) formed in the main body casing (101), wherein a first trigger plate (222) is arranged above the pressing plate (221), a second trigger plate (223) is arranged below the pressing plate (221), a piston rod (225) is slidably connected inside the cavities (224), a second elastic piece (226) is arranged inside the cavities (224), and an air suction groove (227), an air inflation groove (228) and a diversion groove (229) are formed inside the main body casing (101).
  10. 10. The measurement module and the chemiluminescent immunoassay analyzer of claim 9 wherein the pressure plate (221) is slidably connected to an inner wall of the first chute (12), a bottom of the first trigger plate (222) is abutted to a top of the pressure plate (221), the piston rod (225) is elastically connected to an inner wall of the cavity (224) through a second elastic member (226), one end of the piston rod (225) far away from the second elastic member (226) penetrates through the inner wall of the cavity (224) to extend into the first chute (12), one side of the first trigger plate (222) far away from the second trigger plate (223) is fixedly connected to the piston rod (225), an air suction groove (227) is formed in a side surface of the first trigger plate (222), an air charging groove (228) is formed in a side surface of the second trigger plate (223), the two cavities (224) are respectively communicated with an inner part of the shunt groove (229) through the air suction groove (227) and the air charging groove (228), and the air charging groove (228) are both communicated with the shunt tube (217) through the air suction groove (227).

Description

Measurement module and chemiluminescence immunoassay analyzer Technical Field The invention relates to the technical field of chemiluminescent immunoassay, in particular to a measuring module and a chemiluminescent immunoassay instrument. Background The core principle of the chemiluminescent immunoassay technology is that a superparamagnetic bead is used as a carrier of an immune complex based on specific immune binding reaction of an antigen and an antibody, a specific antibody/antigen fixed on the surface of the magnetic bead captures an object to be detected in a sample, and then a detection reagent marked with a tracer luminescent substance (such as acridinium ester, alkaline phosphatase, horseradish peroxidase and the like) is combined to form the immune complex of the magnetic bead-the object to be detected-the luminescent marker, and the chemiluminescent substrate is added to trigger the luminescent reaction subsequently, so that the photon counter (PMT) is used for collecting the optical signal intensity, and further quantitative analysis of the concentration of the object to be detected is realized. The measurement module is used as a core signal acquisition unit of the chemiluminescent immunoassay analyzer, has the core functions of realizing the accurate positioning of immune complex magnetic beads and the efficient capturing of optical signals, and the distribution state (such as whether offset, adherence, stacking and bottom adherence) of the magnetic beads, the sufficiency of luminescent reaction and the purity of detection environment directly determine the accuracy and stability of optical signal acquisition, and is a key link affecting the detection performance of the whole machine. However, the measurement module and the whole machine of the existing chemiluminescent immunoassay analyzer still have a plurality of technical defects in practical application, and the technical defects are as follows: The magnetic field of the existing measurement module is mostly static in design, the magnetic field intensity and distribution cannot be dynamically adjusted according to the requirements of different stages such as transfer of a reaction cup, addition of a substrate, detection and collection, and when the magnetic field is detected, either the magnetic beads are offset, attached to the wall or stacked at the edge due to insufficient magnetic field intensity, or the magnetic beads are excessively focused and stacked due to excessively strong central magnetic field peak, and the luminous surfaces on the surfaces of the magnetic beads are blocked by each other due to abnormal magnetic bead distribution, so that the optical signal collection error is caused, and the stability and the accuracy of detection are seriously influenced. In view of this, we propose a measurement module and a chemiluminescent immunoassay analyzer. Disclosure of Invention The invention aims to provide a measuring module and a chemiluminescent immunoassay analyzer, which solve the technical problems in the background technology. The technical scheme includes that the measuring module and the chemiluminescent immunoassay analyzer comprise a measuring component, wherein the measuring component comprises an analyzer main body, an injection assembly for injecting trace substances is arranged in the analyzer main body, a first chute and a second chute are arranged in the analyzer main body, and a measuring assembly for detecting chemiluminescent signals in a reaction cup is arranged in the analyzer main body; tiling part, it is including setting up the regulation subassembly in the analyzer main part for adjust the magnetic bead in the reaction cup, be provided with the shielding component that is used for weakening main magnetic path magnetic field in the regulation subassembly, be provided with the drive assembly that is used for adjusting the shielding component in the analyzer main part. As an alternative scheme of the file technical scheme, the analyzer main body comprises a main body shell, a feeding groove is formed in the side face of the main body shell, a conveyor belt is arranged in the feeding groove, a plurality of clamping grooves are formed in the conveyor belt, clamping blocks are clamped in the clamping grooves, and reaction cups are arranged on the side face of the clamping blocks; the conveyer belt runs through the feed chute, the size of reaction cup and the size looks adaptation of feed chute, the reaction cup is located the inside of feed chute, first spout has been seted up to the inside of main part shell, first spout is located the top of feed chute. As an alternative scheme of the technical scheme of the invention, the injection assembly comprises a motor arranged at the top of a main body shell, a screw rod is arranged below the motor, a sliding plate is connected to the screw rod in a threaded manner, and an injection head is arranged at the bottom of the sliding plate; the screw rod penetrates through