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CN-122017250-A - TSP-1 detection device

CN122017250ACN 122017250 ACN122017250 ACN 122017250ACN-122017250-A

Abstract

The invention provides TSP-1 detection equipment which is characterized by comprising a sensing core module, wherein the sensing core module takes Metglas alloy as a substrate, and after chromium and Jin Gaixing are carried out on the surface of the sensing core module, a TSP-1 antibody is attached to the sensing core module. The device is a novel detection scheme with high sensitivity, high specificity, low cost and portability, realizes rapid and accurate detection of TSP-1, has detection limit (12.857 ng pair mL ‑1 ) and linear range (12.857-100 ng pair mL ‑1 ) which are completely matched with OA clinical diagnosis requirements, avoids interference of external factors such as electromagnetic interference and light source influence, can be directly applied to detection of actual joint fluid samples of OA patients, provides an efficient technical means for early diagnosis and disease prognosis monitoring of OA, reduces detection cost and improves convenience and practicability of clinical detection.

Inventors

  • CHE XIANDA
  • WEI XIAOCHUN
  • CAO FUYANG
  • LI PENGCUI
  • LI LU
  • ZHANG CHENGMING
  • ZHANG GUOHAO
  • WU GAIGE

Assignees

  • 山西医科大学第二医院(山西医科大学第二临床医学院)

Dates

Publication Date
20260512
Application Date
20260204

Claims (10)

  1. 1. A TSP-1 detection device is characterized by comprising a sensing core module; The sensing core module takes Metglas alloy as a substrate, and after chromium and Jin Gaixing are carried out on the surface, the TSP-1 antibody is attached to the sensing core module.
  2. 2. The TSP-1 detection apparatus of claim 1, wherein: and the chromium and the gold are sequentially deposited on the surface of the substrate to form a chromium layer and a gold layer.
  3. 3. The TSP-1 detection apparatus of claim 1, wherein: The thickness of the chromium layer and the gold layer is 50-150nm.
  4. 4. The TSP-1 detection apparatus of claim 1, wherein: the surface-modified Metglas alloy is subjected to amination treatment.
  5. 5. The TSP-1 detection apparatus of claim 1, wherein: The TSP-1 antibody is activated and then bonded to the modified alloy.
  6. 6. The TSP-1 detection apparatus of claim 1, wherein: The specific preparation method of the sensing core module comprises the following steps: S1, ME chip pretreatment and modification: S1.1, cutting a Metglas alloy material to a target size; S1.2, sequentially depositing a chromium layer and a gold layer on the surface of the bare chip by adopting a plasma sputtering technology; S1.3, cleaning the chip to remove surface impurities; s2.TSP-1 antibody immobilization: S2.1, amination treatment, namely horizontally immersing the chip modified by the S1 into an amination reagent, and incubating at room temperature to form amino active sites on the surface of the chip; s2.2, activating the antibody, namely activating the TSP-1 antibody under the action of an antibody activating reagent to obtain an activated antibody solution; S2.3, antibody fixation, namely immersing the chip subjected to S2.1 amination into an activated antibody solution formed by S2.2, and incubating to realize covalent bonding of amino and activated carboxyl to realize antibody fixation; S2.4, cleaning and blocking, namely after washing the chip by using a buffer solution to remove the nonspecific binding antibodies, immersing the chip into a blocking reagent to block the nonspecific binding sites.
  7. 7. The TSP-1 detection apparatus of claim 1, wherein: the system also comprises a signal detection module; The signal detection module comprises a glass tube wrapping a coil, an analyzer and a direct current bias magnet; the sensing core module is arranged in the glass tube; The coil wound outside the glass tube is electrically connected with the analyzer to generate an alternating magnetic field; the direct current bias magnet is arranged on the outer side of the glass tube and used for maximizing the vibration amplitude of the sensor.
  8. 8. Use of a TSP-1 detection device as claimed in any one of claims 1 to 7 for quantitatively detecting TSP-1 in an ex-vivo sample.
  9. 9. The use according to claim 8, wherein: the ex-vivo sample is an ex-vivo serum or joint fluid sample.
  10. 10. Use of a TSP-1 detection device as claimed in any one of claims 1-7 as an OA early diagnosis device.

Description

TSP-1 detection device Technical Field The invention relates to the technical field of biological medicines, in particular to a TSP-1 detection device, and specifically relates to TSP-1 magneto-elastic test paper. Background The existing early diagnosis of Osteoarthritis (OA) has the problems that the traditional imaging technology can only detect late OA, and cannot realize early screening, and the early diagnosis of OA depends on high-sensitivity detection of a marker thrombospondin (TSP-1), but the existing TSP-1 detection method (such as an enzyme-linked immunoassay method and the like) has the defects of complex operation, high preparation cost, time consumption for detection, unmatched clinical requirements of linear range and the like, and is difficult to meet the requirements of clinical convenience and accurate detection, and the method comprises the following specific steps: the existing method I is an enzyme-linked immunoassay method. The method has the advantages of complex operation flow, long detection period, high equipment and reagent cost and unsuitability for rapid detection and field application scenes. Existing methods two, immunohistochemical and biochemical assays. The method cannot capture low-concentration markers in early patients and has no early screening capability at all. Aiming at the problems that the existing method is poor in portability, depends on large laboratory equipment and professional operating environments, cannot realize clinical field detection, primary medical screening or postoperative bedside monitoring, and has unmatched practicality, such as disjointed detection range (linear range and detection limit) and OA clinical diagnosis requirements, or cannot detect low-concentration early markers or cannot cover middle-high-concentration pathological states, so that the full-course monitoring requirements are difficult to meet. In addition, the existing scheme has the problem of weak anti-interference capability, namely, part of optical fiber sensors and electrochemical sensors are easily influenced by external factors such as electromagnetic interference, light source fluctuation, environmental medium corrosion and the like, and the detection accuracy and reliability are difficult to guarantee. In addition, the unbalance of the cost and the efficiency is also a key which leads to the fact that the scheme cannot be popularized at present. That is, the existing technology is either expensive to detect or time-consuming to operate, and cannot meet the requirements of economy and rapidity of clinical application. Disclosure of Invention The invention aims to overcome the defects, provides a novel detection scheme with high sensitivity, high specificity, low cost and portability, realizes rapid and accurate detection of TSP-1, has detection limits (12.857 ng, mL-1) and linear ranges (12.857-100 and ng, and mL-1) which are completely matched with the clinical diagnosis requirements of OA, simultaneously avoids the interference of external factors such as electromagnetic interference, light source influence and the like, can be directly applied to the detection of actual joint fluid samples of an OA patient, provides a high-efficiency technical means for early diagnosis and disease prognosis monitoring of the OA, reduces the detection cost and improves the convenience and practicability of clinical detection. The invention provides TSP-1 detection equipment which is characterized by comprising a sensing core module; And the sensing core module takes Metglas alloy as a substrate, and after chromium and Jin Gaixing are carried out on the surface of the sensing core module, the TSP-1 antibody is attached to the sensing core module. Further, the TSP-1 detection device is characterized in that chromium and gold are sequentially deposited on the surface of a substrate to form a chromium layer and a gold layer. Further, the TSP-1 detection device is characterized in that the thicknesses of the chromium layer and the gold layer are 50-150nm. Furthermore, the invention provides TSP-1 detection equipment which is further characterized in that the surface-modified Metglas alloy is subjected to amination treatment. Further, the invention provides TSP-1 detection equipment which is characterized in that the TSP-1 antibody is combined with the modified alloy after being subjected to activation treatment. Further, the invention provides TSP-1 detection equipment, which is further characterized in that the specific preparation method of the sensing core module is as follows: S1, ME chip pretreatment and modification: S1.1, cutting a Metglas alloy material to a target size; S1.2, sequentially depositing a chromium layer and a gold layer on the surface of the bare chip by adopting a plasma sputtering technology; S1.3, cleaning the chip to remove surface impurities; s2.TSP-1 antibody immobilization: S2.1, amination treatment, namely horizontally immersing the chip modified by the S1 into an