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CN-122017266-A - Full-automatic sample pretreatment system and method

CN122017266ACN 122017266 ACN122017266 ACN 122017266ACN-122017266-A

Abstract

The invention relates to a full-automatic sample pretreatment system and method, comprising the following steps of firstly, treating a test sample, namely uniformly injecting blood from the same collection source into a plurality of test tubes to form a test tube sample; step two, centrifugal separation test, step three, test result analysis and step four, test sample treatment. According to the invention, the optimal centrifugal parameters are determined by comprehensively calculating the layering coefficient, the target coefficient and the evaluation index through multi-dimensional sample analysis and multi-condition centrifugal test, and factors such as layering effect, target component retention and impurity control of the blood sample are comprehensively considered, so that the sample processing result meets the actual detection requirement, and the accuracy of the experimental result is greatly improved.

Inventors

  • ZHANG LI

Assignees

  • 启东芳景生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260331

Claims (9)

  1. 1. The full-automatic sample pretreatment method is characterized by comprising the following steps of: The first step of test sample treatment, namely uniformly injecting blood from the same collection source into a plurality of test tubes to form test tube samples; Step two, centrifugal separation testing, namely determining a centrifugal rotating speed range and a centrifugal time range according to the separation requirement of blood components, sequentially selecting any centrifugal rotating speed range and any centrifugal time range in the centrifugal rotating speed range and the centrifugal time range, combining the centrifugal rotating speed range and the centrifugal time range, and taking the combined centrifugal rotating speed range and the centrifugal time range as test conditions of test tube samples, and carrying out centrifugal separation treatment on each test tube sample, wherein a plurality of test tube samples are arranged under each test condition, a test group is formed, and the test conditions of each test group are different; Analyzing the test tube sample after centrifugation to obtain a layering coefficient and a target coefficient, comprehensively processing the layering coefficient and the target coefficient to obtain an evaluation index, and determining the optimal centrifugal rotating speed and the optimal centrifugal time according to the evaluation index; The fourth step of sample testing, namely, marking the sample to be tested, wherein the marking content comprises the name of a patient, the sample number, the acquisition date and time, performing centrifugal separation treatment on the sample to be tested at the optimal centrifugal speed and the optimal centrifugal time, analyzing and judging the blood quality of the sample by comparing the standard pattern with the centrifugally separated sample to be tested, and performing test tube cap opening, rotating stand and test operation on the sample to be tested which meets the detection requirement, and refrigerating the rest sample to be tested according to the storage requirement.
  2. 2. The method according to claim 1, wherein the first step specifically comprises the following steps: Uniformly mixing fresh blood from the same collection source in a workbench, accurately measuring the same amount of blood by a pipette according to the specification of a test tube, and uniformly injecting the same amount of blood into a plurality of test tubes which are subjected to autoclaving treatment and provided with scale marks, thereby forming a test tube sample; After the injection is completed, the mouth of the test tube is closed with a sealing test tube stopper and each test tube is numbered.
  3. 3. The fully automated sample pretreatment method according to claim 1, wherein in the second step, each test tube sample is centrifuged, and wherein the centrifugation operation comprises: sequentially placing test tube samples into a centrifugal machine according to a set sequence, and starting the centrifugal machine to carry out centrifugal treatment on each test tube sample; And after the centrifugal separation is finished, taking out the test tube sample from the centrifugal machine when the centrifugal machine stops running.
  4. 4. The fully automated sample pretreatment method according to claim 1, wherein the analyzing the centrifuged test tube sample to obtain the delamination coefficients comprises the following steps: Acquiring test tube sample images in each test group after centrifugal separation, importing the images into image analysis software, distinguishing a plasma layer from a red blood cell mixing region through color recognition and region division functions, and acquiring the area of the plasma layer and the area of the red blood cell mixing region by using the image analysis software; Dividing the area of the red blood cell mixing region by the area of the plasma layer to obtain the red blood cell mixing ratio, sequentially obtaining the red blood cell mixing ratio of each test tube sample in each test group, and then carrying out average calculation to obtain the average value of the red blood cell mixing ratio of the test conditions corresponding to each test group; Obtaining the thickness of the white film layer of the test tube sample in each test group after centrifugal separation by a flow cytometer, calculating the average value of the thickness of the white film layer and the standard difference value of the thickness of the white film layer of the test tube sample in each test group, dividing the standard difference value of the thickness of the white film layer of the test tube sample in each test group by the average value of the thickness of the white film layer, and obtaining the variation coefficient of the test tube sample in each test group; Obtaining the height of the red blood cell layer of the test tube sample in each test group after centrifugal separation, carrying out average value calculation on the height of the red blood cell layer of the test tube sample in each test group to obtain the average value of the height of the red blood cell layer, and recording the average value as the actual height of the red blood cell layer; Obtaining hematocrit before centrifugal separation of test tube samples in each test group and volumes of the test tube samples in each test group through a hematocrit analyzer, multiplying the volumes of the test tube samples in each test group by the corresponding hematocrit to obtain hematocrit of the test tube samples in each test group, carrying out mean calculation to obtain a mean value of the hematocrit under the test conditions corresponding to each test group, and dividing the mean value of the hematocrit by the bottom area of the test tube sample to obtain theoretical sedimentation height; and dividing the theoretical sedimentation height by the theoretical sedimentation height after carrying out difference calculation on the theoretical sedimentation height and the actual erythrocyte height, and obtaining an error coefficient by taking an absolute value.
  5. 5. The method according to claim 4, wherein the mean value, the coefficient of variation and the coefficient of error of the red blood cell mixture ratio are respectively marked as 、 、 Wherein For the purpose of the test group number, And substitutes the formula: Obtaining layering coefficients of each test group Wherein 、 、 Respectively the maximum red blood cell mixing ratio, the maximum variation ratio and the maximum error ratio, 、 、 Respectively the weight factors corresponding to the mean value of the red blood cell mixing ratio, the variation coefficient and the error coefficient of the test tube sample.
  6. 6. The fully automatic sample pretreatment method according to claim 1, wherein the analysis of the centrifuged test tube sample to obtain the target coefficient comprises the following steps: Taking preset chemical substances in blood as target components, measuring the target component content of test tube samples in each test group before centrifugal separation treatment, and marking the target component content as target content before centrifugation; Dividing the post-centrifugation target content of the test tube samples in each test group by the corresponding pre-centrifugation target content to obtain the recovery rate of the test tube samples in each test group, and marking as ; Average value calculation is carried out on the recovery rate of the test tube samples in each test group to obtain the recovery average rate of each test group, and the recovery average rate is marked as Wherein To test the numbering of test tube samples within a test group, ; By the formula: calculating the deviation coefficient of each test group ; Obtaining impurity component content of test tube samples in each test group measured after centrifugal separation treatment, and marking the impurity component content as impurity content after centrifugation; Dividing the centrifuged impurity content of the test tube samples in each test group by the total content corresponding to each test tube sample to obtain impurity coefficients of the test tube samples in each test group; The impurity coefficients of the test tube samples in each test group are subjected to average value calculation to obtain the average value of the impurity coefficients of each test group, and the average value is marked as ; Coefficient of deviation And the mean value of impurity coefficients Substitution formula: Obtaining target coefficients of each test group Wherein 、 Respectively the maximum deviation proportion and the maximum impurity proportion, 、 The weight factors corresponding to the deviation coefficient and the impurity coefficient mean value are respectively adopted.
  7. 7. The method for fully automatic sample preprocessing according to claim 1, wherein the comprehensive processing of the layering coefficient and the target coefficient is performed to obtain an evaluation index, and the optimal centrifugal speed and the optimal centrifugal time are determined according to the evaluation index, and the specific process comprises the following steps: Presetting weight factors of a layering coefficient and a target coefficient, respectively carrying out product calculation on the layering coefficient and the target coefficient and the weight factors corresponding to the layering coefficient and the target coefficient, and summing to obtain an evaluation index, and obtaining the evaluation index of each test group; The evaluation indexes of the test groups are arranged from left to right according to descending order, and after the minimum evaluation index is determined, the centrifugal revolution number and the centrifugal time in the test conditions corresponding to the minimum evaluation index are respectively used as the optimal centrifugal revolution number and the optimal centrifugal time.
  8. 8. The fully automatic sample pretreatment method according to claim 1, wherein in the fourth step, according to the need of sample detection, the sample to be tested, which needs to be refrigerated after centrifugal separation, is refrigerated, and the method specifically comprises the following steps: When the sample to be tested is put into the refrigeration equipment, the staff needs to check the marking information of the sample to be tested again, and the storage position and the refrigeration time of the sample to be tested are registered in detail in the sample to be tested storage record of the refrigeration equipment, wherein the storage record contains the number of the sample to be tested, the name of a patient, the refrigeration starting time, the expected storage duration and the specific position stored in the refrigeration equipment.
  9. 9. A fully automated sample pre-processing system employing the fully automated sample pre-processing method of any one of claims 1-8, the processing system comprising: The centrifugal separation testing module is used for carrying out centrifugal separation treatment on the test tube sample after setting up testing conditions; the test result analysis module is used for analyzing the centrifuged test tube sample to obtain an evaluation index, and obtaining the optimal centrifugal rotating speed and the optimal centrifugal time according to the evaluation index; and the test sample processing module is used for carrying out centrifugal separation processing on the sample by using the optimal centrifugal rotating speed and the optimal centrifugal time and carrying out corresponding processing on the processed sample.

Description

Full-automatic sample pretreatment system and method Technical Field The invention relates to the technical field of medical instruments, in particular to a full-automatic sample pretreatment system and method. Background In the medical instrument industry, the process of injecting an acquired sample to be detected into a special test tube and sealing and storing the sample is called sampling, the sampled sample is the sample to be detected, and a series of operation procedures of the sample to be detected, which are completed by an inspector before the sample to be detected is detected, are collectively called preprocessing. In the pretreatment process, the blood sample is required to be subjected to centrifugal treatment to obtain the convenience for subsequent inspection operation, but in the traditional centrifugal separation process, centrifugal condition selection only has a approximate range, and the optimal centrifugal parameters cannot be accurately determined according to different detection requirements, so that the recovery rate and purity of target components are influenced, and the accuracy and reliability of detection results are interfered. There is a need for a fully automated sample preprocessing system and method that addresses the above-identified issues. Disclosure of Invention The invention aims to solve the problems and provides a fully-automatic sample pretreatment system and a fully-automatic sample pretreatment method. In order to achieve the above purpose, the present invention adopts the following technical scheme: The full-automatic sample pretreatment method comprises the following steps: The first step of test sample treatment, namely uniformly injecting blood from the same collection source into a plurality of test tubes to form test tube samples; Step two, centrifugal separation testing, namely determining a centrifugal rotating speed range and a centrifugal time range according to the separation requirement of blood components, sequentially selecting any centrifugal rotating speed range and any centrifugal time range in the centrifugal rotating speed range and the centrifugal time range, combining the centrifugal rotating speed range and the centrifugal time range, and taking the combined centrifugal rotating speed range and the centrifugal time range as test conditions of test tube samples, and carrying out centrifugal separation treatment on each test tube sample, wherein a plurality of test tube samples are arranged under each test condition, a test group is formed, and the test conditions of each test group are different; Analyzing the test tube sample after centrifugation to obtain a layering coefficient and a target coefficient, comprehensively processing the layering coefficient and the target coefficient to obtain an evaluation index, and determining the optimal centrifugal rotating speed and the optimal centrifugal time according to the evaluation index; The fourth step of sample testing, namely, marking the sample to be tested, wherein the marking content comprises the name of a patient, the sample number, the acquisition date and time, performing centrifugal separation treatment on the sample to be tested at the optimal centrifugal speed and the optimal centrifugal time, analyzing and judging the blood quality of the sample by comparing the standard pattern with the centrifugally separated sample to be tested, and performing test tube cap opening, rotating stand and test operation on the sample to be tested which meets the detection requirement, and refrigerating the rest sample to be tested according to the storage requirement. Preferably, the step one specifically includes the following parts: Uniformly mixing fresh blood from the same collection source in a workbench, accurately measuring the same amount of blood by a pipette according to the specification of a test tube, and uniformly injecting the same amount of blood into a plurality of test tubes which are subjected to autoclaving treatment and provided with scale marks, thereby forming a test tube sample; After the injection is completed, the mouth of the test tube is closed with a sealing test tube stopper and each test tube is numbered. Preferably, in the second step, each test tube sample is subjected to centrifugation, wherein the centrifugation operation includes: sequentially placing test tube samples into a centrifugal machine according to a set sequence, and starting the centrifugal machine to carry out centrifugal treatment on each test tube sample; And after the centrifugal separation is finished, taking out the test tube sample from the centrifugal machine when the centrifugal machine stops running. Preferably, the analysis of the centrifuged test tube sample to obtain a delamination coefficient comprises the following steps: Acquiring test tube sample images in each test group after centrifugal separation, importing the images into image analysis software, distinguishing a plasma layer from a