CN-122017373-A - Portable plant surface potential detection device and method

Abstract

The invention discloses a portable plant surface potential detection device and a method, wherein the device comprises a detection electrode assembly, a portable electrochemical workstation and a terminal computer, the detection electrode assembly is electrically connected with the electrochemical workstation through a shielding conductive wire, and the electrochemical workstation is in bidirectional communication connection with the terminal computer. The invention creatively adopts an open circuit electrochemical method to carry out the surface potential monitoring of plants, replaces the traditional high-price patch clamp equipment with a low-cost portable electrochemical workstation, has portable modularized design for each part of the device, can be quickly assembled and disassembled, and realizes the on-site real-time monitoring of the surface potential of the plant stalks and leaves in the field natural growth state. Under the detection and stimulation parameters of the device, the personal error is effectively reduced, the detection data is accurate and the stability is high, the potential response rule of the plant under the stimulation of fire, injury and the like can be captured, and the device has the characteristics of low cost, portability and high suitability, and has wide application prospect.

Inventors

• SUN LIJUN
• Niu Mengrui
• ZHANG YALI
• SHI JIAN
• FAN SUSU

Assignees

• 南通大学

Dates

Publication Date

20260512

Application Date

20260324

Claims (10)

1. 1. The portable plant surface potential detection device is characterized by comprising a detection electrode assembly, a portable electrochemical workstation and a terminal computer, wherein the detection electrode assembly is electrically connected with the portable electrochemical workstation through a conductive wire, and the portable electrochemical workstation is in two-way communication connection with the terminal computer through a communication data wire.
2. 2. The portable plant surface potential detecting device of claim 1, wherein the detecting electrode assembly comprises a reference electrode, a counter electrode, a working electrode and a fixture for fixing each electrode to the plant surface, the reference electrode, the counter electrode and the working electrode being wire electrodes.
3. 3. The portable plant surface potential detecting device according to claim 2, wherein the wire electrode is made of a platinum wire-platinum wire, a platinum wire-silver wire or a silver wire-silver wire.
4. 4. The portable plant surface potential detecting device according to claim 1, wherein the sampling interval and the detection running time of the portable electrochemical workstation are flexibly set by a terminal computer, and the minimum sampling interval is 0.1s.
5. 5. The portable plant surface potential detecting device of claim 1, wherein the conductive wire is a shielded wire.
6. 6. The portable plant surface potential detection device of claim 1, wherein the terminal computer incorporates electrochemical workstation specific data processing software.
7. 7. The portable plant surface potential detection device of claim 1, wherein the device is adapted for surface potential detection of plant fire stimuli, injury shear stimuli.
8. 8. The portable plant surface potential detecting device according to claim 7, wherein the fire stimulation time is 0.9 to 1.1s, the distance is 0.9 to 1.1cm, and the stimulation interval is 150s to 200s.
9. 9. A portable plant surface potential detection method using the apparatus of claim 1, comprising the steps of: S1, stably attaching a detection electrode assembly to a plant detection part through a fixing piece; S2, connecting the detection electrode assembly with a portable electrochemical workstation, and connecting the portable electrochemical workstation with a terminal computer in a communication way; S3, setting detection parameters and stimulation parameters through a terminal computer; S4, applying preset stimulus to the plants, and starting the portable electrochemical workstation to collect plant surface potential signals; and S5, receiving and processing signals by the terminal computer, and realizing real-time display, storage and analysis of data.
10. 10. The method according to claim 9, wherein the stimulus in the step S4 is one of fire stimulus and injury shear stimulus, and the stimulus parameters include stimulus time, stimulus distance, and stimulus interval.

Description

Portable plant surface potential detection device and method Technical Field The invention relates to the technical field of plant physiological detection, in particular to a portable plant surface potential detection device and method. Background The change of the plant surface potential is a physiological response mechanism of a plant core, and the accurate and real-time monitoring of the plant surface potential is an important foundation for exploring the physiological mechanism of the plant, promoting the accurate development of agriculture and developing the application of plant energy. The most commonly used method for plant electrophysiological monitoring is a patch clamp method, and a patch clamp special instrument is matched for use, so that potential detection at the cell level can be realized, but the technology has the obvious technical defects that firstly, patch clamp equipment is high in manufacturing cost and high in experimental investment cost, and is difficult to realize large-scale and popularization application, secondly, the equipment is huge in size and complex in structure, can only be operated in a laboratory fixed scene, and cannot meet the field monitoring requirements in natural growth environments such as fields and outdoors, and thirdly, the patch clamp method is mainly used for potential detection at the plant cell level, and is difficult to realize the dynamic monitoring of the overall potential of the surfaces of plant stems and leaves quickly, and suitability and detection efficiency are limited. In the prior art, a plant surface potential monitoring means which has low cost, portability and field detection capability is not available, and the actual requirements of plant physiological mechanism field research, crop growth state field monitoring, accurate regulation and control of agricultural production and the like cannot be met. Therefore, the plant surface potential detection device which is low in price, high in portability and capable of realizing field on-site detection is developed, and has important research value and application prospect. Disclosure of Invention The invention aims to provide a portable plant surface potential detection device and a portable plant surface potential detection method, wherein the device adopts an open circuit electrochemical method to replace the traditional patch clamp equipment, has the advantages of low cost, small volume and capability of being quickly assembled on site, can adapt to the surface potential detection of plant stems and leaves under different stimuli, and solves the technical problems that the traditional patch clamp equipment is high in manufacturing cost and cannot be used for field on-site monitoring. The portable plant surface potential detection device comprises a detection electrode assembly, a portable electrochemical workstation and a terminal computer, wherein the detection electrode assembly is electrically connected with the portable electrochemical workstation through a conductive wire, the portable electrochemical workstation is in two-way communication connection with the terminal computer through a communication data wire, the detection electrode assembly is used for collecting surface potential signals when being attached to a plant detection part, the portable electrochemical workstation is used for receiving and primarily processing the surface potential signals, and the terminal computer is used for setting detection parameters, receiving the processed signals and realizing visualization, storage and analysis of data. The detection electrode assembly is connected with the portable electrochemical workstation, the portable electrochemical workstation and the terminal computer realize two-way communication through the communication data line, and the portable electrochemical workstation, the terminal computer and the portable electrochemical workstation are portable components, can be rapidly assembled and disassembled, and are suitable for laboratory and outdoor field detection. The portable electrochemical workstation is a signal receiving and preliminary processing unit, is small in size and convenient to carry, core detection parameters such as sampling interval and detection running time can be set remotely through a terminal computer, the minimum sampling interval can be set to be 0.1s, instantaneous change of surface potential of a plant after being stimulated can be accurately captured, the workstation can receive surface potential analog signals transmitted by a detection electrode assembly, preliminary processing such as signal amplification and conversion is completed, digitized signals are transmitted to the terminal computer for subsequent processing, and meanwhile, the workstation can feed back detection states in real time, so that experimenters can conveniently adjust detection parameters in time. The terminal computer is a control and data processing unit for detection, adopts a por