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CN-122018133-A - Microscope objective lens system and method with large object space field of view and long working distance

CN122018133ACN 122018133 ACN122018133 ACN 122018133ACN-122018133-A

Abstract

The invention relates to the technical field of optical imaging, in particular to a microscope objective system with a large object space field of view and a long working distance and a method. The optical system comprises a microscope objective system, wherein the microscope objective system comprises twenty optical lenses, two solution layers and a cover glass, which are arranged on the same optical axis, and a relay group and an objective lens are sequentially arranged from an object side to an image side. The combination of a large object space view (diameter 7.5 mm-8.5 mm), a long working distance (7.5 mm-8.5 mm), a high collection numerical aperture (0.9-1.1) and a 0.5-0.6 numerical aperture is realized for the first time, the technical contradiction that the resolution is sacrificed for a long working distance and the high collection efficiency is difficult to consider for a large view of the traditional objective lens is solved, and the fine structure analysis requirement of single neuron and dendritic spine level is met.

Inventors

  • Teng Huaijin
  • LI YE
  • ZHANG ZHIJUN

Assignees

  • 新辉润(重庆)光学仪器有限公司

Dates

Publication Date
20260512
Application Date
20260416

Claims (10)

  1. 1. The microscope objective system with large object space view and long working distance comprises a microscope objective system and is characterized by comprising twenty optical lenses, two solution layers and a cover glass, wherein the twenty optical lenses are arranged on the same optical axis, and a relay group and an objective lens are sequentially arranged from an object side to an image side; the relay group comprises a G1 lens group and a G2 lens group; The G1 lens group comprises a surface S1, a lens L2, a lens L3, a lens L4, a lens L5, a lens L6 and a surface S2 from the object side to the image side in sequence; the G2 lens group comprises a surface S2, a lens L7, a lens L8, a lens L9, a lens L10, a lens L11 and a surface S3 from the object side to the image side in sequence; The objective lens sequentially comprises a surface S3, a lens L12, a lens L13, a lens L14, a lens L15, a lens L16, a lens L17, a lens L18, a lens L19, a lens L20, a solution layer L21, a cover glass L22 and a solution layer L23 from the object side to the image side.
  2. 2. The objective lens system for a large object field of view long working distance microscope of claim 1, wherein the surface S2 is a common surface of the G1 lens group and the G2 lens group, and the surface S3 is an engagement surface of the relay group and the objective lens.
  3. 3. A large object field of view, long working distance microscope objective system as claimed in claim 1, wherein said surface S1 is a diaphragm; the lens L1 is a negative focal power lens, the object side is a concave surface, the image side is a convex surface, the lens L2 and the lens L3 form a first group of positive focal power cemented lenses, the object side of the lens L2 is a concave surface, the image side of the lens L3 is a convex surface, the lens L4 and the lens L5 form a second group of positive focal power cemented lenses, the object side of the lens L4 is a convex surface, the image side of the lens L4 is a concave surface, the image side of the lens L5 is a concave surface, the lens L6 is a negative focal power lens, the object side is a concave surface, the image side is a convex surface, the surface S2 is a focal surface of the parallel light passing through the G1 lens group, the lens L7 is a positive focal power lens, the object side is a concave surface, the image side is a convex surface, the lens L8 and the lens L9 form a negative focal power cemented lens, the object side of the lens L8 is a convex surface, the image side of the lens L9 is a concave surface, the object side is a convex surface, the object side of the lens L11 is a negative focal power lens, and the image side of the lens L10 is a concave surface.
  4. 4. The large object field long working distance microscope objective system according to claim 1, wherein the surface S3 is a common surface of the objective lens and the relay group, and the image side surface of L23 is a surface on which the sample is placed in actual use of the microscope objective system, i.e. the object side.
  5. 5. The objective lens system for a large object field of view long working distance microscope according to claim 1, wherein the lens L12 is a negative power lens, the object side is a convex surface, the image side is a concave surface, the lenses L13, L14, L15, L17, L18, L19, L20 are positive power lenses, the lens L16 is a negative power lens, the lenses L13, L14 are convex surfaces on the object side and the image side, the lenses L15, L17, L18, L19 are convex surfaces on the object side and the image side are concave surfaces, the lens L16 is a concave surface on the object side and the image side, and the lens L20 is a convex surface on the object side and the image side is a plane.
  6. 6. A large object field of view long working distance microscope objective system as claimed in claim 1, characterized in that the focal length of the lens L12 and the focal length of the objective lens satisfy-0.25 < <-0.24; The focal length of the lens L13 and the focal length of the objective lens meet 0.26< <0.28; The focal length of the lens L14 and the focal length of the objective lens meet 0.19 </DEG. <0.20; The focal length of the lens L15 and the focal length of the objective lens meet 0.43< <0.44; Lens L16 focal length the focal length of the objective lens satisfies: -1.2< <-1.1; The focal length of the lens L17 and the focal length of the objective lens meet 0.30< <0.40; The focal length of the lens L18 and the focal length of the objective lens meet 0.50< <0.60; The focal length of the lens L19 and the focal length of the objective lens meet 0.30< <0.40; The focal length of the lens L20 and the focal length of the objective lens meet 0.70< <0.80; Wherein f is the focal length of the objective lens, For the focal length of the lens L12, For the focal length of the lens L13, For the focal length of the lens L14, For the focal length of the lens L15, For the focal length of the lens L16, For the focal length of the lens L17, For the focal length of the lens L18, For the focal length of the lens L19, Is the focal length of lens L20.
  7. 7. The objective lens system for a large object field of view long working distance microscope as set forth in claim 1, wherein the solution layers L21, L23 are working medium layers, and the working distance of the objective lens is 7.5 mm-8.5 mm, and the working distance is the distance from the image side surface of the lens L20 to the image side surface of the lens L23.
  8. 8. The large object field of view long working distance microscope objective system according to claim 1, wherein the numerical aperture of the microscope objective system is 0.5-0.6, the collection numerical aperture is 0.9-1.1, and the diameter of a final imaging surface is 7.5 mm-8.5 mm, and the final imaging surface is a surface on which a sample is placed in actual use, namely an object side.
  9. 9. The objective lens system for a large object field of view and long working distance microscope according to claim 2, wherein the effective focal length of the G1 lens group is 100mm, the effective focal length of the G2 lens group is 225mm, 2.25 times of beam expansion is realized by matching G1 and G2, the distance between the surface S2 and the object side surface of the lens L7 is 260mm, and an optical modulation element and a light splitting device can be arranged outside the distance range.
  10. 10. A large object field, long working distance microscope objective method for use in a large object field, long working distance microscope objective system as claimed in any one of claims 1 to 8, comprising the steps of: s1, guiding parallel incident light of a near infrared band of 920 nm-940 nm into a relay group diaphragm surface S1 of a microscope objective system, regulating a light passing aperture through a diaphragm, then, entering a G1 lens group, and converging the parallel light to a focal plane S2 through the synergistic effect of a negative focal power lens and two positive focal power cemented lenses in the G1 lens group to finish primary aberration correction; S2, the light beam at the focal plane S2 is incident to a G2 lens group, 2.25 times of light beam expansion is realized through modulation of a positive focal power lens, a negative focal power cemented lens and a positive and negative focal power single lens in the G2 lens group, and meanwhile, field curvature and distortion are corrected, and optimized parallel light is transmitted to an objective lens through a junction plane S3 of a relay group and the objective lens; S3, the light beam of the joint surface S3 is incident to the objective lens, light beam convergence and near infrared band chromatic aberration correction are completed through nine single lenses with preset focal length ratios in the objective lens, and the light beam is focused on an object space sample area with the diameter of 7.5 mm-8.5 mm after passing through a solution layer L21, a cover glass L22 and a solution layer L23 of the objective lens, so that large-field imaging under the working distance of 7.5 mm-8.5 mm is realized; Wherein, the ratio of the focal length of each lens in the objective lens to the total focal length f of the objective lens satisfies: -0.25< <-0.24; 0.26< <0.28; 0.19< <0.20; 0.43< <0.44; -1.2< <-1.1; 0.30< <0.40; 0.50< <0.60; 0.30< <0.40; 0.70< <0.80; S4, collecting fluorescent signals generated by exciting a sample by near infrared light by using a collecting numerical aperture of 0.9-1.1 of an objective lens, reversely transmitting the fluorescent signals along an original light path, separating the fluorescent signals from excitation light through an external optical splitter between a focal plane S2 and a G2 lens group after passing through the objective lens and a relay group, transmitting the separated fluorescent signals to photoelectric detection equipment, and restoring the separated fluorescent signals into a sample imaging image with high signal-to-noise ratio.

Description

Microscope objective lens system and method with large object space field of view and long working distance Technical Field The invention relates to the technical field of optical imaging, in particular to a microscope objective system with a large object space field of view and a long working distance and a method. Background In neuroscience research, mouse models are the core tools for revealing the mystery of brain structure and function. With rapid development of brain coupling atlas drawing, in vivo neuron imaging, optogenetic intervention and other technologies, the conventional microscope objective increasingly shows key technical bottlenecks in terms of imaging range, working distance and imaging mode in research on mouse brain. First, the limited sample imaging range of conventional objectives is contrary to the need for full brain or large brain area observations pursued by neuroscience research. For example, when the projection map of the neurons of the whole brain slice is drawn, the field of view of a conventional objective lens is small, a large number of complicated slice splicing needs to be carried out, so that the efficiency is extremely low, and the integrity information of a nerve loop is easily damaged due to mechanical displacement and image registration errors. This severely constrains the development of high-throughput, systematic brain science research. Secondly, in critical in-vivo imaging and living body operation scenes, the working distance of an objective lens becomes a decisive factor, on one hand, when observing deep brain regions (such as hippocampus) of anesthetized or awake mice, the front end of the objective lens must pass through craniofacial window, cerebrospinal fluid or an implantable glass slide, and long working distance is a precondition for ensuring physical space and safety; The existing long working distance objective lens generally takes the cost of sacrificing numerical aperture and imaging resolution, is difficult to meet the fine structure analysis requirement of single neuron and even dendritic spine level, furthermore, the importance of infrared imaging capability is increasingly prominent, near infrared wave band has deeper penetrating capability and weaker scattering property in biological tissues, is a key window for carrying out deep brain region in-vivo function imaging (such as two-photon excitation), however, the existing objective lens has the problems of insufficient wave band optimization, low transmittance, imperfect chromatic aberration correction and the like, and limits the sensitivity and signal-to-noise ratio of deep high-resolution imaging. The prior art lacks a microscope objective which can simultaneously meet the large object field of view range, long working distance and high numerical aperture to adapt to in-vivo multi-mode experiments and optimize near infrared bands to support deep functional imaging, so that a large object field of view, long working distance microscope objective system and a method are provided. Disclosure of Invention The invention aims to provide a microscope objective lens system with large object space vision and long working distance and a microscope objective lens method, which solve the problems of small imaging vision, short working distance, small collection numerical aperture and poor near infrared band imaging performance in the prior art, realize the combination of large vision, long working distance and high collection efficiency, and meet the requirements of deep high-resolution imaging and multi-mode experiments in the field of neuroscience. In order to solve the above technical problems, one of the purposes of the present invention is to provide a microscope objective system with a large object field of view and a long working distance, which comprises a microscope objective system, wherein the microscope objective system comprises twenty optical lenses arranged with the same optical axis, two solution layers and a cover glass, and a relay group and an objective lens are sequentially arranged from an object side to an image side; the relay group includes a G1 lens group and a G2 lens group; The G1 lens group comprises a surface S1, a lens L2, a lens L3, a lens L4, a lens L5, a lens L6 and a surface S2 from the object side to the image side in sequence; The G2 lens group sequentially comprises a surface S2, a lens L7, a lens L8, a lens L9, a lens L10, a lens L11 and a surface S3 from the object side to the image side; The objective lens includes, in order from the object side to the image side, a surface S3, a lens L12, a lens L13, a lens L14, a lens L15, a lens L16, a lens L17, a lens L18, a lens L19, a lens L20, a solution layer L21, a cover glass L22, and a solution layer L23. As a further improvement of the present technical solution, the surface S2 is a common surface of the G1 lens group and the G2 lens group, and the surface S3 is a junction surface of the relay group and the objective lens.