CN-122028654-A - Biological memristor for periodontitis detection and preparation method and application thereof

CN122028654ACN 122028654 ACN122028654 ACN 122028654ACN-122028654-A

Abstract

The invention belongs to the technical field of medical appliances, and particularly relates to a biological memristor for periodontitis detection and a preparation method and application thereof. The biological memristor can effectively distinguish normal saliva, gingivitis saliva and I-phase periodontitis saliva through an electrical response test, shows a current response with obvious difference and a larger resistance window, highlights the potential of the biological memristor in early detection of periodontitis, provides a low-cost and convenient solution for real-time oral health monitoring, and opens up a new approach for early diagnosis of oral diseases.

Inventors

ZHANG NAN
ZHOU QIANQIAN
SUN BAI
Gao Rongru
LIU CHE

Assignees

西安交通大学口腔医院

Dates

Publication Date: 20260512
Application Date: 20260226

Claims (8)

1. The biological memristor for periodontitis detection is characterized by sequentially comprising a substrate, an anthocyanin functional layer and an Ag electrode from bottom to top; the thickness of the anthocyanin film layer is 600-620 nm, and the thickness of the Ag electrode is 100-150 nm; The anthocyanin film layer is obtained by spin coating an anthocyanin solution on a substrate, wherein the concentration of anthocyanin in the anthocyanin solution is 0.01 g/mL-0.017 g/mL.
2. The bio-memristor for periodontitis detection of claim 1, wherein the substrate is an FTO glass substrate.
3. A method of manufacturing a bio-memristor for periodontitis detection as defined in claim 2, comprising the steps of: Spin-coating the anthocyanin solution on an FTO glass substrate to obtain an anthocyanin functional layer; depositing an Ag electrode on the anthocyanin functional layer by adopting magnetron sputtering to obtain the biological memristor; The anthocyanin solution is obtained by dissolving anthocyanin and polyvinylidene fluoride in N-methylpyridine-2-formamide according to a mass ratio of 5-10:1.
4. The method according to claim 3, wherein the spin coating is performed at a speed of 100rpm min -1 ~2000rpm·min -1 for 20s to 60s.
5. The method according to claim 3, wherein the parameters of the magnetron sputtering are that the argon gas pressure is 0.4 pa-0.7 pa, the power is 60 w-70 w, and the sputtering time is 600 s-900 s.
6. Use of the biological memristor of claim 2 in oral detection.
7. The use according to claim 6, wherein the oral cavity detection is periodontitis and gingivitis detection.
8. The use according to claim 7, wherein the bio-memristor is placed in saliva to be tested, detected by a change in current.

Description

Biological memristor for periodontitis detection and preparation method and application thereof Technical Field The invention belongs to the technical field of medical appliances, and particularly relates to a biological memristor for periodontitis detection and a preparation method and application thereof. Background The current oral health plays a key role in overall health, affecting basic physiological functions such as chewing, swallowing, speech and the like. Research has shown that oral diseases, particularly periodontitis, not only cause localized pain and dysfunction, but also increase the risk of systemic diseases such as cardiovascular diseases and diabetes. Among the numerous oral diseases, periodontitis is the most common and clinically significant disease. Periodontitis is a chronic inflammatory disease affecting the teeth and their supporting tissues, resulting in alveolar bone resorption, loosening and even loss of teeth. Early detection of periodontitis is of paramount importance, as the effects of the disease can be significantly reduced by timely intervention. In fact, most oral health problems can be effectively prevented and treated at an early stage. Current detection methods are still limited in their ability to detect early stages, particularly before clinical manifestations appear evident. Conventional oral examination relies on careful visual and tactile assessment of teeth, gums, tongue, oral mucosa and salivary glands, and common instruments include dental mirrors, forceps and probes. Taking periodontitis as an example, routine examination typically uses periodontal probes to measure the depth of investigation, loss of adhesion, and bleeding at investigation, which are the basic clinical criteria for judging periodontitis. Meanwhile, X-rays are also widely used to evaluate the level of alveolar bone resorption, helping to determine the severity of the disease. However, these methods have limitations in that the probe measurement is heavily dependent on the experience of the operator and the applied pressure, which may cause deviations, whereas conventional X-ray films generally only reveal significant bone destruction and cannot capture early changes. In recent years, new detection techniques have been applied to early detection of periodontitis. For example, cone Beam Computed Tomography (CBCT) can provide three-dimensional images that more intuitively and accurately assess periodontal tissue and bone mass, however its radiation exposure and high cost limit its use in large-scale population screening. On the other hand, the pathogenesis of periodontal disease is closely related to chronic inflammation caused by bacterial infection, with actinomycetes (a. Actinomycetemcomitans) and porphyromonas gingivalis (p. Gingivalis) being key pathogens. Studies have shown that H 2O2 levels in saliva are significantly higher in periodontitis groups than in healthy control and gingivitis groups. Notably, the concentration of lactic acid in saliva has been shown to be a specific biomarker for assessing the symptomatic state of periodontitis. This phenomenon is closely related to the anaerobic metabolic process of periodontal pathogens, which produce lactic acid by anaerobic metabolism in an anoxic environment. Lactic acid is not easy to be cleared in time in the oral cavity, and thus gradually accumulates, thereby reflecting the inflammation degree of periodontal tissues. In this context, saliva has been explored as a non-invasive method for detecting inflammatory factors and biomarkers associated with periodontitis, showing great potential. Although the existing periodontal inspection method plays an important role in clinical practice, there are still disadvantages in sensitivity of early detection, ease of operation and low cost accessibility. In order to shift from reactive therapy to preventive care, there is an urgent need to develop new technologies that can dynamically capture inflammatory states and tissue destruction. Disclosure of Invention In order to solve the technical problems, the invention provides a biological memristor for periodontitis detection and application thereof. The invention provides a biological memristor for periodontitis detection, which sequentially comprises a substrate, an anthocyanin functional layer and an Ag electrode from bottom to top; the thickness of the anthocyanin film layer is 600-620 nm, and the thickness of the Ag electrode is 100-150 nm; The anthocyanin film layer is obtained by spin coating an anthocyanin solution on a substrate, wherein the concentration of anthocyanin in the anthocyanin solution is 0.01 g/mL-0.017 g/mL. In another preferred embodiment, the substrate is an FTO glass substrate. The second aspect of the invention provides a preparation method of the biological memristor for periodontitis detection, which comprises the following steps: Spin-coating the anthocyanin solution on an FTO glass substrate to obtain an anthocyanin functio