CN-122028911-A - Composition for activating hair follicle stem cells, composition for inhibiting expression of wnt3 gene in hair follicle stem cells, and composition for inhibiting expression of bmp4 gene in hair follicle stem cells

Abstract

The purpose of the present invention is to provide a composition for activating hair follicle stem cells, which contains a natural and safe-approved component that can activate hair follicle stem cells as an active ingredient. The above object is achieved by a composition for activating hair follicle stem cells, which comprises at least 1 st active ingredient selected from the group consisting of 10-hydroxy-2-decenoic acid and derivatives thereof, and salts thereof, and at least 1 st active ingredient selected from the group consisting of 2-decenoic acid and derivatives thereof, and salts thereof.

Inventors

• Hideto Okamoto
• TSUBOKAWA RYO
• OKUMURA HIROAKI
• Yuan Guishi
• FUKADA TOSHIYUKI

Assignees

• 株式会社山田养蜂场本社

Dates

Publication Date

20260512

Application Date

20231117

Priority Date

20230928

Claims (8)

1. 1. A composition for activating hair follicle stem cells, which is characterized in that, Comprises at least 1 st active ingredient selected from the group consisting of 10-hydroxy-2-decenoic acid and its derivatives and salts thereof, and at least 1 st active ingredient selected from the group consisting of 2-decenoic acid and its derivatives and salts thereof, and 2 nd active ingredient.
2. 2. A composition for inhibiting the expression of wnt3 gene in hair follicle stem cells or a composition for inhibiting the expression of bmp4 gene in hair follicle stem cells, characterized in that, Comprises at least 1 st active ingredient selected from the group consisting of 10-hydroxy-2-decenoic acid and its derivatives and salts thereof, and at least 1 st active ingredient selected from the group consisting of 2-decenoic acid and its derivatives and salts thereof, and 2 nd active ingredient.
3. 3. The composition according to claim 1 or 2, wherein, The composition is food, beverage, cosmetic, quasi drug or medicinal product.
4. 4. A method for activating hair follicle stem cells is characterized in that, Comprises applying at least 1 st active ingredient selected from the group consisting of 10-hydroxy-2-decenoic acid and its derivatives and salts thereof, and at least 1 st active ingredient selected from the group consisting of 2-decenoic acid and its derivatives and salts thereof, to a subject to activate hair follicle stem cells of the subject.
5. 5. A method for inhibiting the expression of wnt3 gene in hair follicle stem cells, which is characterized in that, Comprising applying at least 1 st active ingredient selected from the group consisting of 10-hydroxy-2-decenoic acid and derivatives thereof and salts thereof, and at least 1 st active ingredient selected from the group consisting of 2-decenoic acid and derivatives thereof and salts thereof to a subject to inhibit expression of wnt3 gene in hair follicle stem cells of the subject.
6. 6. A method for inhibiting the expression of the bmp4 gene in hair follicle stem cells, characterized in that, Comprising applying at least 1 st active ingredient selected from the group consisting of 10-hydroxy-2-decenoic acid and derivatives thereof and salts thereof, and at least 1 st active ingredient selected from the group consisting of 2-decenoic acid and derivatives thereof and salts thereof to a subject to inhibit expression of the bmp4 gene in hair follicle stem cells of the subject.
7. 7. A use of the composition is characterized in that, The use of at least 1 st active ingredient selected from the group consisting of 10-hydroxy-2-decenoic acid and derivatives thereof and salts thereof, and at least 1 st active ingredient selected from the group consisting of 2-decenoic acid and derivatives thereof and salts thereof, for the manufacture of a composition for at least 1 action selected from the group consisting of activation of hair follicle stem cells, inhibition of expression of wnt3 gene in hair follicle stem cells and inhibition of expression of bmp4 gene in hair follicle stem cells.
8. 8. The use according to claim 7, wherein, The composition is food, beverage, cosmetic, quasi drug or medicinal product.

Description

Composition for activating hair follicle stem cells, composition for inhibiting expression of wnt3 gene in hair follicle stem cells, and composition for inhibiting expression of bmp4 gene in hair follicle stem cells Technical Field The present invention relates to a composition for activating hair follicle stem cells, a composition for inhibiting the expression of wnt3 gene in hair follicle stem cells, and a composition for inhibiting the expression of bmp4 gene in hair follicle stem cells. Background Both the Wnt signaling pathway and the Bmp (Bone Morphogenetic Protein: bone morphogenic protein) signaling pathway are composed of multiple factors, and transmit signals into cells via receptors on the cell surface. These signal pathways all play an important role in the early stages of production, and function in such a manner as to regulate intercellular signals related to differentiation of cells and arrangement of tissues and appropriately control interactions between cells. Wnt3 in the Wnt signaling pathway (WNT FAMILY Member 3: wnt family Member 3) and Bmp4 in the Bmp signaling pathway (Bone Morphogenetic Protein: bone morphogenic protein 4) are known to be involved in the maintenance of stem cell properties of hair follicle stem cells (for example, refer to non-patent documents 1 and 2). Hair follicle stem cells are quiescent until telogen, while maintaining stem cell properties as they are. Hair follicle stem cells proliferate and differentiate while transitioning from telogen to anagen. If the expression levels of Wnt3 protein and Bmp4 protein are reduced, the hair follicle stem cells are released from the resting state and shift from resting phase to growing phase. That is, due to the decrease in Wnt3 protein and Bmp4 protein in the hair follicle stem cells, the hair follicle stem cells in the telogen phase are induced to be activated in a growth phase in such a manner as to promote proliferation of the hair follicle stem cells and promote differentiation of the hair follicle stem cells. Further, as a result of activation of hair follicle stem cells, the proportion of hair follicle stem cells increases. As a therapeutic agent for alopecia and male pattern alopecia, a hair tonic is known. As an active ingredient of hair-growing agents, various substances are known, among which unsaturated fatty acids are known. Namely, hair growth agents containing an unsaturated fatty acid as an active ingredient are known (for example, refer to patent document 1 and non-patent document 3). On the other hand, as a natural-derived substance containing various unsaturated fatty acids, there is royal jelly. 10-hydroxy-2-decenoic acid and 10-hydroxydecanoic acid contained in royal jelly are known to be effective for improving female alopecia (for example, refer to patent document 2). Prior art literature Patent literature Patent document 1 Japanese patent application laid-open No. 2017-81962 Patent document 2 Japanese patent No. 6808737 Non-patent literature Non-patent document 1:Plikus MV et al, nature 2008 Jan 17; 451 (7176): 340-4. Non-patent document 2, lim X et al, proc NATL ACAD SCI USA 2016 Mar 15; 113 (11): E1498-505. Non-patent document 3 Brotzu G et al Dermatol Ther.2019 Jan; 32 (1): e12778. Disclosure of Invention Problems to be solved by the invention The substances described in patent documents 1 and 2 and non-patent document 3 are useful for the treatment or improvement of alopecia, male pattern alopecia and female pattern alopecia. However, these documents do not describe that the active ingredients described in these documents can activate hair follicle stem cells. In addition, it has been hardly known to date that the hair follicle stem cells are derived from natural and safe components that are approved and activated. Accordingly, the present invention has been made to solve the problems described above, and an object of the present invention is to provide a composition for activating hair follicle stem cells, which contains a natural and safe-approved component capable of activating hair follicle stem cells as an active ingredient. The present invention also provides a composition for inhibiting the expression of wnt3 gene in hair follicle stem cells and a composition for inhibiting the expression of bmp4 gene in hair follicle stem cells, which can activate hair follicle stem cells, as the problems to be solved by the present invention. Solution for solving the problem The present inventors have made intensive studies to solve the above problems, and have attempted to obtain an active ingredient for activating hair follicle stem cells by combining a plurality of ingredients individually or appropriately. As a result of repeated studies, it has been found that a combination of 10-hydroxy-2-decenoic acid and 2-decenoic acid can activate hair follicle stem cells by inhibiting the expression of wnt3 gene in hair follicle stem cells and inhibiting the expression of bmp4 gene in hair follicle stem cells. Fu