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CN-122029227-A - Stable bacterial cellulose suspensions

CN122029227ACN 122029227 ACN122029227 ACN 122029227ACN-122029227-A

Abstract

The present invention relates to stable bacterial cellulose suspensions, BC powders with good redispersibility, compositions comprising the same, and methods of making and using the same.

Inventors

  • D. Fapian
  • A. Pelson
  • M. Mengqijia

Assignees

  • 赛璐吉有限责任公司

Dates

Publication Date
20260512
Application Date
20240823
Priority Date
20230824

Claims (20)

  1. 1. A processed Bacterial Cellulose (BC) suspension having an acetate to BC ratio (acetate to BC ratio) of at least 0.1:1, and: a. At least 0.2:1, such as at least 0.3:1, such as at least 0.4:1, such as at least 0.5:1, such as at least 0.8:1, such as at least 1:1, such as at least 1.1:1, such as at least 1.2:1, such as at least 1.5:1, such as at least 1.6:1, such as at least 1.7:1, such as at least 2:1, such as at least 2.1:1, such as at least 2.2:1, such as at least 2.5:1, such as at least 2.7:1, such as at least 2.8:1, such as at least 2.9:1, for example at least 3:1, for example at least 3.5:1, for example at least 4:1, for example at least 4.5:1, for example at least 4.6:1, for example at least 4.7:1, for example at least 5:1, for example at least 5.5:1, for example at least 6:1, for example at least 6.5:1, for example at least 7:1, for example at least 7.5:1, for example at least 8:1, for example at least 8.5:1, for example at least 9:1, for example at least 9.5:1, for example at least 10:1, or higher; and/or B. At most 10:1, such as at most 9.5:1, such as at most 9:1, such as at most 8.5:1, such as at most 8:1, such as at most 7.5:1, such as at most 7:1, such as at most 6.5:1, such as at most 5.5:1, such as at most 5:1, such as at most 4.7:1, such as at most 4.6:1, such as at most 4.5:1, such as at most 4:1, such as at most 3.5:1, such as at most 3:1, such as at most 2.9:1, such as at most 2.8:1, such as at most 2.7:1, such as at most 2.5:1, such as at most 2.2:1, such as at most 2:1, such as at most 1.7:1, such as at most 1.1, such as at most 1.1:1, such as at most 1, such as at most 1.1:1, such as at most 1:1, such as at most 4.5:1, such as at most 4:1, such as at most 1:1:1, such as at most 1.5:1, such as at most 1:1, such as at most 0.1:1, such as at most 0.1, such as at most 0.1:1, such as at most 1:1, such as at most 1:1:1) C. Between 0.1:1 and 10:1, such as between 0.5:1 and 7.5:1, such as between 1:1 and 5:1, such as between 1:1 and 4.6:1, such as between 1.1:1 and 4:1, such as between 2:1 and 3:1, such as between 2.1:1 and 2.8:1.
  2. 2. The processed BC suspension according to any one of the preceding claims, wherein said acetate comprises at least the monosaccharides glucose (Glc) and mannose (Man), and optionally rhamnose (Rha), arabinose (Ara), xylose (Xyl), galactose (Gal), uronic acid (UrA) and/or glucuronic acid (GlcA).
  3. 3. The processed BC suspension of any one of the preceding claims, wherein said BC suspension further comprises levan and/or levan.
  4. 4. The processed BC suspension according to any one of the preceding claims, wherein said acetate comprises at least three different monosaccharides, such as at least four different monosaccharides, such as five different monosaccharides, wherein said monosaccharides comprise at least Glc and Man, preferably wherein said acetate comprises four different monosaccharides, including Glc, man, rha, urA and GlcA or consists of Glc, man, rha, urA and GlcA.
  5. 5. A suspension of processed BC according to any one of the preceding claims, comprising between 0.02% and 3% (w/w) BC solids, wherein said suspension: a. Containing BC fiber clusters having a size between 50 nm and 3000 μm, such as between 50 nm and 2500 μm; b. having a viscosity of between 0.5 and 5000 mPa s at a shear stress of 100/s at 25 ℃, and/or a viscosity of between 5 and 250 000 mPas at a shear stress of 1/s at 25 ℃, such as between 1000 and 200 000, such as between 2000 and 150 000, such as between 3000 and 100 000, such as between 5000 and 60 000 mPas, and C. Has a water retention capacity of at least 80 g water/g BC.
  6. 6. A suspension of processed BC according to any one of the preceding claims, wherein said suspension: It is stable and therefore has the advantage that, It is a smooth surface, which is not a smooth surface, The particles are not agglomerated, Non-flocculating, and/or No water-cellulose separation is exhibited.
  7. 7. A suspension of processed BC according to any one of the preceding claims, wherein said suspension: a. Comprises between 0.02% and 2.8% (w/w) BC solids, such as between 0.02% and 2.4% (w/w) BC solids, such as between 0.02% and 2% (w/w) BC solids, such as between 0.03% and 2.8% (w/w) BC solids, such as between 0.03% and 2.4% (w/w) BC solids, such as between 0.03% and 2% (w/w) BC solids, such as between 0.04% and 2.8% (w/w) BC solids, such as between 0.04% and 2.4% (w/w) BC solids, such as between 0.04% and 2% (w/w) BC solids; b. Comprising BC clusters having a size between 50nm and 2000 μm, such as between 50nm and 1700 μm, such as between 60 nm and 1300 μm, such as between 65 nm and 1200 μm, such as between 65 nm and 1100 μm, such as between 70 nm and 1000 μm, such as between 50nm and 500 μm, such as between 50nm and 300 μm, such as between 50nm and 200 μm, such as between 65 nm and 200 μm, such as between 70 nm and 150 μm; c. Having a water retention capacity of at least 90g water/g BC (g/g), such as at least 100 g/g, such as at least 110 g/g, such as at least 120 g/g, such as at least 130 g/g, such as at least 140 g/g, such as at least 150 g/g, such as at least 160 g/g, such as at least 170 g/g, such as at least 180 g/g, such as at least 190 g/g, such as at least 200 g/g; d. The crystallinity index of BC is at least 65%, such as at least 70%, such as at least 75%, such as at least 80%, such as at least 85%, when measured by Fourier Transform Infrared (FTIR) spectroscopy.
  8. 8. The processed BC suspension of any one of the preceding claims, wherein: a. The suspension has a viscosity of between 0.5 and 2000 mPa s under a shear stress of 25 ℃, 100/s, for example between 0.5 and 1mPa s under a shear stress of 25 ℃, 100/s, for example between 20 and 100 mPa s, for example between 300 and 1000 mPa s, for example 1500 mPa s, for example between 10 and 1000 mPa s, for example between 5 and 1000 mPa s, and/or B. The suspension has a viscosity of between 10 000 and 220 000 mPa-s under a shear stress of 25 ℃, 1/s, for example between 70 and 220 000 mPa-s under a shear stress of 25 ℃, 1/s, for example between 10 and 40 000, for example between 12 and 35 000 mPa-s, for example between 15 and 35 000 mPa-s, for example between 16 and 30 000 mPa-s; Optionally, wherein the viscosity is measured at a BC solids concentration of 1% (w/w).
  9. 9. The processed BC suspension of any one of the preceding claims, wherein said suspension is capable of stabilizing particles in a composition.
  10. 10. The processed BC suspension according to any one of the preceding claims, wherein said suspension is capable of stabilizing particles in a composition, such as an aqueous composition, such as a composition comprising water, when the BC solids content of said suspension is between 0.02% (w/w) and 10%, such as between 0.03% (w/w) and 7.5%, such as between 0.035% (w/w) and 6%, such as between 0.04% (w/w) and 5% (w/w), or such as BC solids content is between 0.02% (w/w) and 0.4% (w/w).
  11. 11. The processed BC suspension according to any one of the preceding claims, wherein said suspension is capable of stabilizing particles in a composition, such as an aqueous composition, such as a suspension comprising water, when said composition has a BC solids content of at most 0.04% (w/w).
  12. 12. The processed BC suspension of any preceding claim, wherein said suspension comprises impurities, Optionally, wherein the impurity is an impurity having a neutral charge or having a negative charge, Optionally, wherein the impurity is one or more compounds selected from the group consisting of: A protein; Nitrogen; ions and salts thereof, such as calcium, sodium, potassium, magnesium and salts thereof, such as calcium chloride, calcium carbonate, calcium sulfate, sodium chloride, sodium carbonate, sodium sulfate, potassium chloride, potassium sulfate, potassium carbonate, magnesium chloride, magnesium carbonate or magnesium sulfate, and Silicon and its derivatives, such as silica, silicate, siloxane or silicon dioxide.
  13. 13. The processed BC suspension of any one of the preceding claims, wherein said suspension has: a. At least 0.05 wt%, such as at least 0.1 wt%, such as at least 0.15 wt%, such as at least 0.2 wt%, and/or b. at least 0.05 wt%, such as at least 0.1 wt%, such as at least 0.15 wt%, such as at least 0.2 wt%, of ions and salts thereof and/or silicon and derivatives thereof.
  14. 14. The processed BC suspension of any one of the preceding claims, wherein said composition is: a. Personal care products, cosmetics, pharmaceuticals, biomedical products or foods, and/or B. Ingredients for personal care products, cosmetics, pharmaceuticals, biomedical products or food products.
  15. 15. The processed BC suspension of any one of the preceding claims, wherein said processed BC suspension does not comprise viable bacteria, proteins or DNA.
  16. 16. The processed BC suspension of any one of the preceding claims, wherein said suspension is stable: a. at a pH between 2 and 13, for example in a composition having a pH between 2 and 13; b. Optionally in a composition comprising the BC suspension and between 5 and 35% (w/w) of an oil, for example between 10 and 20% (w/w) of an oil, optionally wherein the oil is a polar oil or a non-polar oil selected from the group consisting of paraffinic oil and jojoba oil, in the presence of an oil, for example in a composition comprising the BC suspension and an oil; c. Optionally in the presence of a salt, e.g. in a composition comprising a BC suspension and a salt, optionally in a composition comprising a BC suspension and between 5 and 25% (w/w) of a salt, e.g. between 5 and 15% (w/w) of a salt, optionally wherein the salt is a sodium or calcium salt, preferably selected from the group consisting of sodium chloride, sodium citrate, sodium dihydrogen phosphate and sodium dihydrogen phosphate; d. Optionally in the presence of a surfactant, e.g. in a composition comprising a BC suspension and a surfactant, optionally in a composition comprising a BC suspension and between 5 and 25% (w/w) of a surfactant, e.g. between 5 and 15% (w/w), optionally wherein the surfactant is a cationic surfactant, an anionic surfactant or a zwitterionic surfactant, e.g. selected from the group consisting of cocamidopropyl betaine (CAPB), sodium Dodecyl Sulfate (SDS), sodium Laureth Sulfate (SLS), lauryl glucoside and a surfactant comprising a quaternary ammonium cation (QUAT), and/or E. Optionally in a composition comprising the BC suspension and between 5 and 70% (v/v) ethanol, e.g. between 20 and 60% (w/w) ethanol, in the presence of ethanol, e.g. in a composition comprising the BC suspension and ethanol.
  17. 17. A method of preparing an acetate-containing processed BC suspension, the method comprising the steps of: a. incubating a cellulose-producing bacterium in a medium, wherein the cellulose-producing bacterium produces an acetate-containing BC, thereby obtaining a BC-containing fermentation broth; b. treating the BC containing fermentation broth to obtain a processed fermentation broth containing a processed BC suspension, and C. Recovering the acetate-containing processed BC suspension from the processed fermentation broth, thereby obtaining the acetate-containing processed BC suspension, Wherein the acetate-containing processed BC suspension is as defined in any one of claims 1 to 14, Further, the ratio of acetate to BC (acetate: BC ratio) in said BC suspension containing acetate therein is as defined in claim 1.
  18. 18. The method according to claim 17, wherein step b. Does not significantly remove and/or degrade acetate from said BC and/or processed BC suspension containing acetate.
  19. 19. The method according to any one of claims 17 to 18, wherein step b does not comprise: -bleaching, for example treating the BC-comprising fermentation broth and/or the processed fermentation broth with a bleaching agent, such as H 2 O 2 and/or NaClO 2 /NaClO and/or NaOH; Coarse filtration, for example coarse centrifugation and/or coarse filtration, for example filtration with a filter of >1 μm, optionally in combination with washing, and/or -Treating the BC comprising fermentation broth and/or the processed fermentation broth, optionally wherein the alkali treatment is repeated more than once, for example with a strong alkali, for example sodium hydroxide having a concentration of higher than 0.5 to 2M, for example 0.5 to 1M, and/or repeating a base treatment, for example with a base, for example sodium hydroxide having a concentration of lower than 0.5M, more than once.
  20. 20. The method according to any one of claims 17 to 19, wherein the method does not comprise the step of adding and/or mixing any additional synthetic and/or anionic polymer, such as pectin, carboxymethyl cellulose, hydroxyethyl cellulose, xanthan gum, alginate, gellan gum (gellan gum), diutan gum (diutan gum), welan gum (welan gum), rhamnose gum (rhamsan gum), carrageenan, guar gum, agar, acacia gum, pedicel gum (gum ghatti), karaya gum (karaya gum), tragacanth gum, tamarind gum or locust bean gum, to the BC-containing fermentation broth, BC-containing processed fermentation broth and/or the acetate-containing processed BC suspension.

Description

Stable bacterial cellulose suspensions Technical Field The present invention relates to stable bacterial cellulose suspensions, BC powders with good redispersibility, compositions comprising the same, and methods of making and using the same. Background Bacterial Cellulose (BC) is an extracellular polysaccharide, usually produced by Acetobacter (Acetobacter)/Gluconobacter (Gluconobacter)/colpitis (Komagataeibacter) by converting sugar (glucose, sucrose, etc.), and can be recovered by purifying and recovering insoluble fiber components. When produced in a dynamic fermenter, the resulting BC consists of a variety of cellulose fibers (from very fine cellulose fibers with a width of 40 to 100 nanometers to larger fibers with a length of 100-150 microns or more). Chemically, BC is identical to cellulose of vegetable origin, such as microfibrillated cellulose (MFC). However, the high aspect ratio, crystallinity and purity of BC fibers distinguish the material from MFC. Due to their unique physical properties, BC fibers tend to form highly reticulated network structures, resulting in high viscosity and yield stress systems in aqueous liquids or solutions. Accordingly, BC has a variety of application prospects as rheology modifiers in personal care, household care, and food products (e.g., thickeners and stabilizers of solids in water or formulation mixtures). Furthermore, because crystalline cellulose is very resistant to harsh environments (e.g., high temperatures, extreme pH, salts, and surfactants), the use of BC in the formulation under the above conditions is advantageous over other biopolymers that are more sensitive to these conditions. Differently modified polyacrylic acids (known as carbomers) are currently the most commonly used rheology modifiers in personal care products. These are anionic polymers which are effective thickeners for aqueous suspensions at low concentrations (< 1 wt%), resulting in high viscosity and smooth gels. Especially carbomers, are desirable in their organoleptic properties and tend to overcome some of the disadvantages of these polymers, such as being incompatible with cationic surfactants and low pH, and being less prone to biodegradation. Regarding carbomers, the most urgent problem is that they are not natural and can accumulate in nature (Rozman and Kalcikov a, 2021). Thus, many industries are currently looking for green alternatives. There are a wide variety of natural polymers used as rheology modifiers, such as proteins and polysaccharides. Nevertheless, these polymers are often used to modify food products, and are not so widely used in personal care products. Because of their undesirable organoleptic properties (tacky, greasy or sticky feel), incompatibility with common personal care ingredients, or stability problems (time, temperature or pH). In view of the above, BC has the potential to replace carbomers in personal care products due to its high viscosity, non-tacky/non-greasy/non-tacky organoleptic properties, and stability under a wide range of conditions. Nevertheless, the most prominent problem with BC and MFC as rheology modifiers is that the fibers are insoluble in water. Thus, a large amount of energy is required to disperse the fibers by mixing, and even so, cellulose-water separation and agglomeration occur with time or at high shear rates. This severely limits the application of BC and MFC, especially in personal care products where organoleptic properties are highly important. For example, MFC dispersions have been reported to produce a "stringy feel" after application to the skin due to fiber aggregation. Furthermore, MFC solutions are known to be unstable and to separate easily into a cellulosic fraction and a water fraction, which limits their application, especially in personal care products. To address the problem of BC insolubility, one of two strategies is generally employed: 1) Chemical modification of cellulose hydroxyl groups, e.g. by TEMPO-oxidation (Saito et al, 2007; jun et al, 2019), or 2) Co-precipitation with reagents (e.g. CMC or other negatively charged polymers) (WO 2006/127810). In case 1) an important processing step is added, involving hazardous chemicals such as hypochlorite and exacerbating scalability. In case 2), CMC is regarded as a cellulose derivative, which, due to being highly chemically modified, is regarded as a semisynthetic substance, which prevents natural labeling. Natural polysaccharides such as xanthan, chitin or pectin have also been shown to disperse BC. Nevertheless, large amounts of these polymers, e.g.30-50% of the dry content, are usually required to achieve the desired effect. This results in diminished performance of the final BC suspension in terms of organoleptic properties, viscosity, solids stability, and stability in various systems. Disclosure of Invention The invention is defined in the claims. The invention described herein relates to stable Bacterial Cellulose (BC) suspensions, BC powders with good re