CN-122029237-A - Method for dissolving poorly water-soluble protein, protein solution obtained by the method, and molded article thereof

Abstract

The present invention provides a method for dissolving a poorly water-soluble protein, and a molded article produced using the protein solution obtained by the method. The present invention is a method for dissolving a poorly water-soluble protein, comprising a step of mixing a hydroxyl-containing polymer, a poorly water-soluble protein and an ionic liquid. The present invention also enables the use of the poorly water-soluble protein solution obtained by this method to produce a molded article.

Inventors

• Katsuji Xiaoda
• Gu Heqitai
• Jin Wuzuo

Assignees

• 富尔玛株式会社

Dates

Publication Date

20260512

Application Date

20241015

Priority Date

20231016

Claims (20)

1. 1. A method for dissolving a poorly water-soluble protein, comprising a step of mixing a hydroxyl-containing polymer, a poorly water-soluble protein and an ionic liquid.
2. 2. The method of claim 1, further comprising the step of mixing a co-solvent.
3. 3. The method of claim 2, wherein the co-solvent is a surfactant.
4. 4. A method according to claim 3, wherein the surfactant is a cationic surfactant with a super acid as a counter ion.
5. 5. The method of claim 3, wherein the surfactant is present in an amount of about 0.05 wt.% to about 10 wt.% based on 100 wt.% of the total weight of the poorly water soluble protein and hydroxyl-containing polymer.
6. 6. The method of claim 1, wherein the poorly water soluble protein is present in an amount of about 5% to about 90% by weight based on 100% by weight of the total weight of the poorly water soluble protein and hydroxyl-containing polymer.
7. 7. The method of claim 1, wherein the poorly water soluble proteins include eggshell membrane, wool, chicken feathers, silk and scales, and their partial hydrolysates and/or partial reduction products.
8. 8. The method of claim 1, wherein the ionic liquid comprises an imidazolium cation, a pyridinium cation, and an alkylammonium cation.
9. 9. The method of claim 1, wherein the hydroxyl-containing polymer comprises a modified hydroxyl-containing polymer having functional groups incorporated on the hydroxyl-containing polymer.
10. 10. The method of claim 1, wherein the hydroxyl-containing polymer comprises a saponified of cellulose, polyvinyl alcohol, and polyvinyl acetate.
11. 11. A lysate obtained by dissolving a hydroxyl-containing polymer and a poorly water-soluble protein in an ionic liquid.
12. 12. The lysate of claim 11, further comprising a cosolvent.
13. 13. The lysate of claim 12, wherein the co-solvent is a surfactant.
14. 14. The lysate of claim 13, wherein the surfactant is a cationic surfactant that counter-ions a super acid.
15. 15. The lysate of claim 13 wherein the surfactant is present in an amount of about 0.05% to about 10% by weight based on 100% by weight of the total weight of the poorly water soluble protein and hydroxyl-containing polymer.
16. 16. The lysate of claim 11 wherein the poorly water soluble protein is present in an amount of about 5% to about 90% by weight based on 100% by weight of the total weight of the poorly water soluble protein and hydroxyl-containing polymer.
17. 17. The lysate of claim 11, wherein the poorly water soluble proteins include eggshell membranes, wool, chicken feathers, silk and scales, and their partial hydrolysates and/or partial reduction products.
18. 18. The lysate of claim 11, wherein the ionic liquid comprises an imidazolium cation, a pyridinium cation, and an alkylammonium cation.
19. 19. The solubilizate of claim 11, wherein the hydroxyl-containing polymer comprises a modified hydroxyl-containing polymer having a functional group incorporated on the hydroxyl-containing polymer.
20. 20. The lysate of claim 11 wherein the hydroxyl-containing polymer comprises saponified species of cellulose, polyvinyl alcohol, and polyvinyl acetate.

Description

Method for dissolving poorly water-soluble protein, protein solution obtained by the method, and molded article thereof Technical Field The present invention relates to a method for dissolving a poorly water-soluble protein and a protein solution obtained by the method, and more particularly, to a method for dissolving a hydroxyl-containing polymer and a poorly water-soluble protein in an ionic liquid, a poorly water-soluble protein solution obtained by the method, and a molded article thereof. Background In recent years, the sustainable development target (SDGs) has been attracting attention. In order to move to sustainable society, efforts are being made to effectively utilize the past abandoned resources and to maintain a rich environment without damaging the earth. As one of the above, it is desired to prepare a uniform solution of a poorly water-soluble protein, which is a natural product that is difficult to recycle and is discarded, and a hydroxyl-containing polymer that is a natural product, and to recycle the solution as a fiber and/or film-like molded product. Disclosure of Invention Means for solving the problems The inventors of the present invention found a method for dissolving a poorly water-soluble protein in order to realize recycling, in addition to effectively utilizing waste resources. Furthermore, the present inventors have found a method of extracting and solubilizing a poorly water-soluble protein in the form of a poorly water-soluble protein in a high molecular region to exhibit a novel function without lowering the molecular weight of the poorly water-soluble protein by hydrolysis. Therefore, the invention provides a method for dissolving water-insoluble proteins by using ionic liquid. According to a main aspect of the present invention, there is provided the following invention: (item 1) A method for dissolving a poorly water-soluble protein, comprising a step of mixing a hydroxyl-containing polymer, a poorly water-soluble protein and an ionic liquid. (Item 2) The method according to any one of the preceding claims, further comprising the step of mixing a cosolvent. (Item 3) A method according to any preceding claim, wherein the co-solvent is a surfactant. (Item 4) The method according to any one of the preceding claims, wherein the surfactant is a cationic surfactant having a super acid as a counter ion. (Item 5) The method according to any one of the preceding claims, wherein the surfactant is present in an amount of about 0.05 wt.% to about 10 wt.%, based on 100 wt.% of the total weight of the poorly water soluble protein and the hydroxyl-containing polymer. (Item 6) The method according to any one of the preceding claims, wherein the poorly water soluble protein is present in an amount of about 5% to about 90% by weight based on 100% by weight of the total weight of the poorly water soluble protein and the hydroxyl-containing polymer. (Item 7) The method according to any one of the preceding claims, wherein the poorly water soluble proteins include eggshell membrane, wool, chicken feathers, silk and scales, and their partial hydrolysates and/or partial reduction products. (Item 8) A method according to any preceding claim, wherein the ionic liquid comprises an imidazolium cation, a pyridinium cation and an alkylammonium cation. (Item 9) The method according to any one of the preceding claims, wherein the hydroxyl-containing polymer comprises a modified hydroxyl-containing polymer having functional groups bonded to the hydroxyl-containing polymer. (Item 10) The method according to any one of the preceding claims, wherein the hydroxyl group-containing polymer comprises a saponified product of cellulose, polyvinyl alcohol and polyvinyl acetate. (Item 11) A lysate obtained by dissolving a hydroxyl-containing polymer and a poorly water-soluble protein in an ionic liquid. (Item 12) The lysate of any preceding claim, further comprising a cosolvent. (Item 13) The lysate according to any one of the preceding claims, wherein the cosolvent is a surfactant. (Item 14) The lysate according to any one of the preceding items, wherein the surfactant is a cationic surfactant having a super acid as a counter ion. (Item 15) The lysate of any one of the preceding claims, wherein the surfactant is present in an amount of about 0.05 wt% to about 10 wt%, based on 100 wt% of the total weight of the poorly water soluble protein and the hydroxyl-containing polymer. (Item 16) The lysate of any one of the preceding claims, wherein the poorly water-soluble protein is present in an amount of about 5% to about 90% by weight, based on 100% by weight of the total weight of the poorly water-soluble protein and the hydroxyl-containing polymer. (Item 17) The lysate of any preceding claim, wherein the poorly water soluble proteins comprise eggshell membrane, wool, chicken feathers, silk and scales, and partial hydrolysates and/or partial reduction products thereof. (Item 18) A lysate according to any preceding clai