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CN-219449702-U - Separation culture apparatus of hair follicle stem cells

CN219449702UCN 219449702 UCN219449702 UCN 219449702UCN-219449702-U

Abstract

The utility model discloses a device for separating and culturing hair follicle stem cells, which comprises a culture bottle body, an upper cover and a connecting bottleneck, wherein the upper cover is in threaded connection with the connecting bottleneck, an angle adjusting structure is arranged between the culture bottle body and the connecting bottleneck, the angle adjusting structure comprises a first spherical surface, the first spherical surface is arranged at the top end of the culture bottle body, and a second spherical surface is arranged at the bottom end of the connecting bottleneck; according to the separating and culturing device for the hair follicle stem cells, provided by the utility model, when the culture medium is added into the bottle body of the culture bottle, the hair follicle stem cells can be directly poured, and particularly, when the hair follicle stem cells are decomposed, some instruments need to be stretched into the bottle body of the culture bottle, so that the inclined upper cover can be avoided, the instruments cannot be stretched into the bottle body of the culture bottle, the rotating angle can be limited, the first spherical surface and the second spherical surface are prevented from falling off during rotation, and the sealing gasket of the limiting groove can reduce the leakage of liquid.

Inventors

  • ZHU XUELIANG
  • LUO JINGUO
  • CHEN XUELING
  • HE XIAOMING
  • ZHU TAO

Assignees

  • WUHAN JINBODA HEALTH MAN CO LTD

Dates

Publication Date
20230801
Application Date
20230410
Priority Date
20230410

Claims (6)

  1. 1. An isolated culture device of hair follicle stem cells, which is characterized in that: including blake bottle body (1), upper cover (2) and connecting bottleneck (5) threaded connection, be provided with angle adjustment structure (3) between blake bottle body (1) and connecting bottleneck (5), angle adjustment structure (3) include first sphere (31), first sphere (31) set up in blake bottle body (1)'s top, the bottom of connecting bottleneck (5) is provided with second sphere (32), first sphere (31) and second sphere (32) overlap each other, reserve groove (33) have been seted up to the edge of second sphere (32), the surface of first sphere (31) is provided with a pair of connecting pin (35), a pair of connecting hole (34) have been seted up on the surface of second sphere (32), and connecting pin (35) of first sphere (31) are rotated with connecting hole (34) of second sphere (32) and are connected.
  2. 2. The isolated culture device of hair follicle stem cells according to claim 1, wherein: the centers of the first spherical surface (31) and the second spherical surface (32) are overlapped, and the first spherical surface (31) and the second spherical surface (32) are in spherical connection.
  3. 3. The isolated culture device of hair follicle stem cells according to claim 2, wherein: the straight line of the connecting hole (34) passes through the sphere center of the second sphere (32), and the straight line of the connecting pin (35) passes through the sphere center of the first sphere (31).
  4. 4. A device for isolated culture of hair follicle stem cells according to claim 3, wherein: the shape of the reserved groove (33) is a crescent surface, and the surface of the reserved groove (33) is coplanar with the sphere center of the second sphere (32).
  5. 5. The isolated culture device of hair follicle stem cells according to claim 2, wherein: be provided with seal structure (4) between first sphere (31) and second sphere (32), seal structure (4) are including spacing step (41), and spacing step (41) are as an organic whole with the top of blake bottle body (1), two sets of spacing groove (44) have been seted up to the outer wall of first sphere (31), and the inside of spacing groove (44) all is provided with sealed pad (42), and the inner wall of second sphere (32) is provided with two sets of ladder ring (43), and ladder ring (43) and spacing groove (44) position looks adaptation.
  6. 6. The isolated culture device of hair follicle stem cells of claim 5, wherein: diameter phase adaptation of ladder ring (43) and spacing groove (44), and block between ladder ring (43) and the spacing groove (44) of first sphere (31) of second sphere (32).

Description

Separation culture apparatus of hair follicle stem cells Technical Field The utility model relates to the field of hair follicle stem cells, in particular to a device for separating and culturing hair follicle stem cells. Background Hair follicle stem cells are one of the hair follicle outer root sheath bulge parts of human, have multidirectional differentiation potential, can differentiate into epidermis, hair follicle and sebaceous glands and participate in the process of skin wound healing, and the hair follicle stem cell culture has important significance. According to the Chinese patent application number: the method for separating and culturing the rat hair follicle stem cells described in CN201310290238.8, under the patent name of "method for separating and culturing the rat hair follicle stem cells", is characterized by comprising the following steps of: 1) Selecting one week old SD rat tentacle skin … …, rinsing with PBS, placing into a 50mL culture flask, adding DMEM/F12 to supplement culture medium, allowing follicular protuberance to climb out to form clone after 1 week, horizontally standing at room temperature for 5min to uniformly distribute lysate on cell surface, blowing cells with a pipette to make cells fall off, wherein culture medium is required to be added into the phase culture flask for isolation culture environment, and later liquid in the culture flask is required to be poured into a culture dish for blowing hair follicle stem cells with the pipette; however, the common separation culture flask has a certain disadvantage, and because the flask body needs to be fixed in some places and can not incline or the inclination is limited by working space, the arm can block the sight, and at the moment, the dumping culture solution is easy to spill from the flask mouth, especially when the hair follicle is treated, the hair follicle stem cells are blown by using a pipetting gun, and the common inclined bottleneck needs to influence the entering of an instrument, so that the end part of the instrument can not extend into the bottom end of the culture flask. Disclosure of Invention The utility model mainly aims to provide a device for separating and culturing hair follicle stem cells, which can effectively solve the technical problems in the background technology. In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows: the utility model provides a hair follicle stem cell's separation culture apparatus, includes blake bottle body, upper cover and connecting bottleneck threaded connection, be provided with angle adjustment structure between blake bottle body and the connecting bottleneck, angle adjustment structure includes first sphere, first sphere sets up in the top of blake bottle body, the bottom of connecting the bottleneck is provided with the second sphere, first sphere and second sphere mutually overlap, and the reservation groove has been seted up to the edge of second sphere, the surface of first sphere is provided with a pair of connecting pin, a pair of connecting hole has been seted up to the surface of second sphere, and the connecting pin of first sphere rotates with the connecting hole of second sphere to be connected. As a preferable technical scheme of the utility model, the sphere centers of the first sphere and the second sphere are coincided, and the first sphere and the second sphere are connected. As a preferable technical scheme of the utility model, the straight line of the connecting hole passes through the center of the second spherical surface, and the straight line of the connecting pin passes through the center of the first spherical surface. As a preferable technical scheme of the utility model, the shape of the reserved groove is a crescent surface, and the surface where the reserved groove is positioned is coplanar with the sphere center of the second spherical surface. As a preferable technical scheme of the utility model, a sealing structure is arranged between the first spherical surface and the second spherical surface, the sealing structure comprises a limiting step, the limiting step is arranged at the top end of the body of the culture bottle, two groups of limiting grooves are formed in the outer wall of the first spherical surface, sealing gaskets are arranged in the limiting grooves, two groups of stepped rings are arranged on the inner wall of the second spherical surface, and the stepped rings are matched with the limiting grooves in position. As a preferable technical scheme of the utility model, the diameters of the stepped ring and the limiting groove are matched, and the stepped ring of the second sphere is clamped with the limiting groove of the first sphere. Compared with the prior art, the utility model has the following beneficial effects: the utility model is provided with the angle adjusting structure, so that the culture medium can be directly poured when being added into the culture bottle