CN-224199369-U - Production fungus jar is used in methyl bacillus cultivation

CN224199369UCN 224199369 UCN224199369 UCN 224199369UCN-224199369-U

Abstract

The utility model provides a production fungus pot for culturing methyl bacilli, which belongs to the technical field of fungus culture and comprises a fungus pot body, wherein a pot cover is rotatably connected to the top wall of the fungus pot body, symmetrically distributed feeding pipes are fixedly connected to the top wall of the pot cover, handles are fixedly connected to the top wall of the pot cover, a driving motor B is fixedly connected to the outer wall of the fungus pot body, and a discharge port is fixedly connected to the outer wall of the fungus pot body. According to the utility model, the extrusion hollow plate is driven to rotate by the driving motor B, so that the air outlet holes are periodically aligned with the ventilation cylinder, the pulse spraying of mixed gas is realized, denser microbubbles can be formed, the contact area of the gas and thalli is increased, the dissolution of oxygen and methane is accelerated, the mass transfer efficiency is remarkably improved, the metabolic activity of the methylobacterium is promoted, the linkage design of the sealing frame and the conical spring is matched, the reverse flow of the gas is avoided, and the stability and the controllability of the aeration process are ensured.

Inventors

CHAO YUANQING
QIU RONGLIANG
Jin Wanyi
YIN YALIN
ZHANG YUN
JIANG GENGBO
Xu Gangqi
WANG TANFEI
WANG SHIZHONG
TANG YETAO

Assignees

中山大学

Dates

Publication Date: 20260505
Application Date: 20250528

Claims (5)

1. The utility model provides a production fungus jar for bacillus methylotrophicus cultivation, includes fungus jar body (1), its characterized in that, fungus jar body (1) roof rotates and is connected with cover (5), inlet pipe (7) of cover (5) roof fixedly connected with symmetric distribution, cover (5) roof fixedly connected with handle (6), fungus jar body (1) outer wall fixedly connected with driving motor B (15), fungus jar body (1) outer wall fixedly connected with bin outlet (2), still include: The gas lift flow reaction assembly comprises an inner bottom plate (20) fixedly connected to the inner wall of the fungus pot body (1), ventilation barrels (26) uniformly distributed are fixedly connected to the outer wall of the inner bottom plate (20), sliding frames (28) are fixedly connected to the inner wall of the ventilation barrels (26), conical springs (29) are sleeved on the outer walls of the sliding frames (28), sealing frames (30) are slidably connected to the outer walls of the sliding frames (28), the outer walls of the sealing frames (30) are abutted against the inner walls of the ventilation barrels (26), the sealing frames (30) are fixedly connected with the conical springs (29), and extrusion rods (27) are fixedly connected to the outer walls of the sealing frames (30); The rotary gas transmission assembly is arranged on the inner wall of the fungus pot body (1) and is matched with the airlift reaction assembly; The reciprocating movement detection assembly is arranged on the top wall of the fungus pot body (1).
2. The production fungus jar for bacillus methylotrophicus cultivation of claim 1, wherein the rotary gas transmission assembly comprises a fixed sealing seat (19) fixedly connected with the inner wall of the fungus jar body (1), the outer wall of the fixed sealing seat (19) is rotationally connected with a rotary sealing disc (21), the outer wall of the rotary sealing disc (21) is fixedly connected with an extrusion hollow plate (22), the outer wall of the extrusion hollow plate (22) is fixedly connected with the output end of a driving motor B (15), uniformly distributed gas outlet holes (24) are formed in the top wall of the extrusion hollow plate (22), a plug-in preventing plate (25) is fixedly connected with the outer wall of the gas outlet holes (24), uniformly distributed gas inlet holes (23) are formed in the outer wall of the rotary sealing disc (21), and the gas inlet holes (23) are positioned on the inner wall of the fixed sealing seat (19).
3. The production fungus jar for bacillus methylotrophicus cultivation according to claim 1, wherein the reciprocating detection assembly comprises a driving motor A (8) fixedly connected to the top wall of the fungus jar body (1), the output end of the driving motor A (8) is fixedly connected with a reciprocating screw rod (9), the outer wall of the reciprocating screw rod (9) is rotationally connected with a symmetrically distributed locating plate (10), the locating plate (10) is fixedly connected with the fungus jar body (1), symmetrically guide rods (11) symmetrically distributed are fixedly connected between the locating plates (10), the outer wall of the guide rods (11) is slidably connected with a mounting plate (12), the outer wall of the mounting plate (12) is propped against the outer wall of the reciprocating screw rod (9), the outer wall of the mounting plate (12) is fixedly connected with a methanol concentration sensor (13), and the outer wall of the mounting plate (12) is fixedly connected with an oxygen sensor (14).
4. The production fungus pot for culturing the methylobacterium according to claim 1, wherein the outer wall of the fungus pot body (1) is fixedly connected with symmetrically distributed support columns (3), and the outer wall of the support column (3) is fixedly connected with a support plate (4).
5. The production fungus pot for culturing the methylobacterium according to claim 1, wherein the outer wall of the fungus pot body (1) is fixedly connected with an L-shaped plate (16), the outer wall of the L-shaped plate (16) is fixedly connected with a pump (17), a water outlet of the pump (17) is fixedly connected with an input pipe (18), the input pipe (18) is fixedly connected with the fungus pot body (1), and the input pipe (18) is communicated with a fixed sealing seat (19).

Description

Production fungus jar is used in methyl bacillus cultivation Technical Field The utility model relates to the technical field of fungus cultivation, in particular to a production fungus pot for culturing methylobacterium. Background The methylobacterium is a gram-negative bacterium with special metabolic capability, can utilize one-carbon compounds such as methanol, methane and the like as unique carbon sources and energy sources for growth and reproduction, and has important application value in the fields of biodegradation, biofuel synthesis, plant growth promotion and the like. Because the metabolic characteristics depend on strict gas environment (such as the accurate ratio of methane to oxygen) and even nutrition distribution, the adoption of a production fungus tank for large-scale culture becomes a key way for industrial application. Parameters such as temperature, gas concentration, stirring rate and the like can be accurately regulated and controlled through fungus pot culture, the activity and metabolic efficiency of the methylobacterium are guaranteed, and high-density and high-yield cultivation is realized. However, the existing bacillus methylotrophicus culture fungus tanks have some problems, namely 1, an aeration link, a traditional continuous aeration mode is easy to cause uneven gas distribution and overlarge bubble size, so that the dissolution efficiency of oxygen and methane is low, the special requirements of bacillus methylotrophicus on a carbon source and dissolved oxygen are difficult to meet, 2, in a detection link, most fungus tanks are arranged by adopting fixed-point sensors, concentration gradient changes caused by fungus liquid layering cannot be adapted, errors exist in monitoring the concentration of methanol and the oxygen content of different liquid layers, and further, the accurate regulation and control of culture parameters are difficult to realize, so that the growth efficiency of thalli is low, and the fluctuation of metabolite yield is large. Therefore, there is a need for a production tank for culturing methylobacterium to solve the above problems. Disclosure of utility model The utility model aims to provide a production fungus pot for culturing methyl bacillus, which solves the problems in the background technology. In order to achieve the above object, the present utility model provides the following technical solutions: The utility model provides a production fungus jar for bacillus methylotrophicus cultivation, includes fungus jar body, fungus jar body roof rotates and is connected with the cover, cover top wall fixedly connected with symmetric distribution's inlet pipe, cover top wall fixedly connected with handle, fungus jar body outer wall fixedly connected with driving motor B, fungus jar body outer wall fixedly connected with bin outlet still includes: The air lift flow reaction assembly comprises an inner bottom plate fixedly connected to the inner wall of the fungus pot body, air exchange tubes which are uniformly distributed are fixedly connected to the outer wall of the inner bottom plate, a sliding frame is fixedly connected to the inner wall of the air exchange tubes, conical springs are sleeved on the outer wall of the sliding frame, a sealing frame is slidably connected to the outer wall of the sliding frame, the outer wall of the sealing frame abuts against the inner wall of the air exchange tubes, the sealing frame is fixedly connected with the conical springs, and an extrusion rod is fixedly connected to the outer wall of the sealing frame; The rotary gas transmission assembly is arranged on the inner wall of the fungus pot body and is matched with the airlift flow reaction assembly; The reciprocating movement detection assembly is arranged on the top wall of the fungus pot body. As the preferable technical scheme of the application, the rotary gas transmission assembly comprises a fixed sealing seat fixedly connected with the inner wall of the fungus pot body, the outer wall of the fixed sealing seat is rotationally connected with a rotary sealing disc, the outer wall of the rotary sealing disc is fixedly connected with an extrusion hollow plate, the outer wall of the extrusion hollow plate is fixedly connected with the output end of a driving motor B, the top wall of the extrusion hollow plate is provided with uniformly distributed gas outlet holes, the outer wall of the gas outlet holes is fixedly connected with an anti-plugboard, the outer wall of the rotary sealing disc is provided with uniformly distributed gas inlet holes, and the gas inlet holes are positioned on the inner wall of the fixed sealing seat. As the preferable technical scheme of the application, the reciprocating movement detection assembly comprises a driving motor A fixedly connected to the top wall of the fungus pot body, the output end of the driving motor A is fixedly connected with a reciprocating screw, the outer wall of the reciprocating screw is rotationally connecte