Search

CN-224199403-U - High-precision organ chip model with temperature control function

CN224199403UCN 224199403 UCN224199403 UCN 224199403UCN-224199403-U

Abstract

The utility model discloses a high-precision organ chip model with a temperature control function, which comprises a top cover, a cell culture chamber, a bottom cover, a nanowire, a thin film resistance heater and a temperature control unit, wherein a plurality of cell culture chambers which are mutually communicated are arranged between the top cover and the bottom cover. The utility model overcomes the defect that the mode is not applicable to a multi-organ chip or a tumor hyperthermia chip in the prior art, for example, a multi-organ chip for liver-kidney-intestine combination is used, an intestinal reservoir (top) is required to simulate 37 ℃ intestinal cavity liquid, a liver reservoir (middle) is required to maintain 37 ℃ metabolic environment, a kidney reservoir (bottom) is cooled to 25 ℃ to simulate urine discharge, a tumor area of the tumor hyperthermia chip is required to be quickly heated to 43 ℃, and a surrounding normal tissue area (adjacent reservoir) is required to be maintained at 37 ℃. Thereby providing a high-precision organ-chip model with a temperature control function capable of dynamically adjusting temperatures of a plurality of regions of an organ chip.

Inventors

  • LU JIE

Assignees

  • 南京翼扬生物医学科技有限公司

Dates

Publication Date
20260505
Application Date
20250527

Claims (7)

  1. 1. A high-precision organ chip model with a temperature control function is characterized by comprising a top cover (1), a cell culture chamber (2), a bottom cover (3), a nanowire (4), a thin film resistance heater and a temperature control unit (5), wherein a plurality of cell culture chambers (2) which are mutually communicated are arranged between the top cover (1) and the bottom cover (3), a plurality of temperature control units (5) which are in one-to-one correspondence with each cell culture chamber (2) are arranged on one side of the top cover (1), and the two opposite ends of the nanowire (4) are respectively connected with the corresponding temperature control units (5) and the corresponding cell culture chambers (2), and one end, far away from the temperature control units (5), of the nanowire (4) is provided with the thin film resistance heater.
  2. 2. The high-precision organ-chip model with temperature control function according to claim 1, characterized in that the nanowire (4) is an Ag nanowire and the outer surface thereof is covered with an AgCl nanowire.
  3. 3. The high-precision organ-chip model with temperature control function of claim 1, wherein the thin film resistive heater is specifically a thin film platinum resistive heater.
  4. 4. The high-precision organ-chip model with the temperature control function according to claim 1, wherein a plurality of top middle reservoirs (101) which are communicated with each cell culture chamber (2) in a one-to-one correspondence are arranged on the top cover (1), a plurality of bottom middle reservoirs (301) which are communicated with each cell culture chamber (2) in a one-to-one correspondence are arranged on the bottom cover (3), and a plurality of micro-channels (302) which can communicate each bottom middle reservoir (301) are arranged on the bottom cover (3).
  5. 5. The high-precision organ-chip model with temperature control function according to claim 4, wherein a plurality of negative pressure units (6) which are communicated with the reservoir (301) in each bottom in a one-to-one correspondence manner through tubules are arranged on one side of the bottom cover (3).
  6. 6. The high-precision organ-chip model with temperature control function according to claim 5, wherein the negative pressure unit (6) is embodied as a micro peristaltic pump or a syringe.
  7. 7. The high-precision organ-chip model with temperature control function according to claim 1, wherein the top cover (1) and the bottom cover (3) are made of transparent materials.

Description

High-precision organ chip model with temperature control function Technical Field The utility model relates to the field of organ bionic and micro-environment reconstruction micro-fluidic chips, in particular to a high-precision organ chip model with a temperature control function. Background Organ-on-a-chip (Organ-on-a-chip) is a micro-fluidic chip bionic system which is prepared based on micro-processing technology and simulates the complex microstructure, micro-environment and physiological functions of specific organs of human body, and is also called as a micro-physiological system (Microphysiological system). It is a new experimental method which can be used for biomedical research such as drug evaluation and disease model construction. Chinese patent CN201721402861.8 discloses an additional device for controlling temperature and humidity of a microfluidic chip in real time, and the temperature control device is used for controlling temperature of an organ chip body directly through a circulating gas inlet and outlet. The applicant found the following technical problems when implementing the above technical solutions: the method is not applicable to a multi-organ chip or a tumor hyperthermia chip, for example, a multi-organ chip for liver-kidney-intestine combination, wherein intestinal reservoir (top) is required to simulate 37 ℃ intestinal cavity liquid, liver reservoir (middle) is maintained in 37 ℃ metabolic environment, kidney reservoir (bottom) is cooled to 25 ℃ to simulate urine discharge, and tumor area of the tumor hyperthermia chip is required to be quickly heated to 43 ℃, and surrounding normal tissue area (adjacent reservoir) is maintained in 37 ℃. Therefore, it is an urgent need to solve the problem of the present utility model to provide a high-precision organ-chip model with a temperature control function capable of dynamically adjusting temperatures of a plurality of regions of an organ chip. Disclosure of utility model Aiming at the technical problems, the utility model aims to overcome the defect that the mode is not applicable to a multi-organ chip or a tumor hyperthermia chip in the prior art, for example, a multi-organ chip for liver-kidney-intestine combination is needed, an intestinal reservoir (top) is needed to simulate 37 ℃ intestinal cavity liquid, a liver reservoir (middle) is needed to maintain a 37 ℃ metabolic environment, a kidney reservoir (bottom) is cooled to 25 ℃ and simulate urine discharge, a tumor area of the tumor hyperthermia chip is needed to be quickly heated to 43 ℃, and a surrounding normal tissue area (adjacent reservoir) is needed to be maintained at 37 ℃. Thereby providing a high-precision organ-chip model with a temperature control function capable of dynamically adjusting temperatures of a plurality of regions of an organ chip. In order to achieve the aim, the utility model provides a high-precision organ chip model with a temperature control function, which comprises a top cover, a cell culture chamber, a bottom cover, a nanowire, a thin film resistance heater and a temperature control unit, wherein a plurality of cell culture chambers which are mutually communicated are arranged between the top cover and the bottom cover, a plurality of temperature control units which are in one-to-one correspondence with each cell culture chamber are arranged on one side of the top cover, the opposite ends of the nanowire are respectively connected with the corresponding temperature control units and the cell culture chambers, and the thin film resistance heater is arranged at one end of the nanowire far away from the temperature control units. Preferably, the nanowire is an Ag nanowire and its outer surface is covered with an AgCl nanowire. Preferably, the thin film resistive heater is specifically a thin film platinum resistive heater. Preferably, the top cover is provided with a plurality of top middle reservoirs communicated with each cell culture chamber in a one-to-one correspondence manner, the bottom cover is provided with a plurality of bottom middle reservoirs communicated with each cell culture chamber in a one-to-one correspondence manner, and a plurality of micro-channels capable of communicating the bottom middle reservoirs. Preferably, one side of the bottom cover is provided with a plurality of negative pressure units which are communicated with the reservoir in each bottom in a one-to-one correspondence manner through thin tubes. Preferably, the negative pressure unit is in particular a micro peristaltic pump or a syringe. Preferably, the top cover and the bottom cover are made of transparent material. According to the technical scheme, compared with the prior art, the intelligent temperature control system has the advantages that a plurality of normal tissues and a tumor area culture chamber are cultured in a cell culture chamber, a thin film resistance heater is heated through a nanowire by an independently arranged temperature control unit, so tha