CN-224203027-U - Detection device for density gradient centrifugal sample

CN224203027UCN 224203027 UCN224203027 UCN 224203027UCN-224203027-U

Abstract

The utility model provides a detection device for a density gradient centrifugal sample, and belongs to the technical field of detection devices. The utility model comprises a sample sucking platform mechanism, a sample detecting chamber, a sample detecting device and a sample detecting device, wherein the sample sucking platform mechanism is used for sucking layered samples to be detected in a centrifuge tube layer by layer and conveying the layered samples to the sample detecting chamber of the optical detector mechanism; the optical detector mechanism is provided with a xenon lamp light source and a beam shrinking light path system and supports the detection of the full wavelength of 200nm-1100 nm; and the automatic collection platform mechanism is used for sub-packaging the samples detected by the optical detector mechanism. According to the utility model, the separation system and the collection system are integrated into a continuous flow, so that the artificial interference is reduced, and the resolution and the efficiency are improved. By adopting the xenon lamp light source and the beam shrinking light path system, the detection precision and stability are improved based on the principle of a spectrophotometer, the detection wavelength can be freely selected, and the limitation of the traditional dual wavelength is broken through.

Inventors

LI WENQI
WANG CHAOXING
ZHANG TIANYI
XIAO YUBO

Assignees

北京质胜康华科技有限公司
清华大学

Dates

Publication Date: 20260505
Application Date: 20250401

Claims (10)

1. A detection apparatus for density gradient centrifugation samples, comprising: the sample sucking platform mechanism is used for sucking layered samples to be detected in the centrifuge tube layer by layer and conveying the layered samples to a sample detection chamber of the optical detector mechanism; the optical detector mechanism is provided with a xenon lamp light source and a beam shrinking light path system and supports the detection of the full wavelength of 200nm-1100 nm; And the automatic collection platform mechanism is used for sub-packaging the samples detected by the optical detector mechanism.
2. The detection apparatus for density gradient centrifugation samples as claimed in claim 1, wherein the sample sucking platform mechanism comprises: the base is arranged on a platform of the sample sucking platform mechanism; The hydraulic support is arranged on the base; The sliding table is connected to the hydraulic support in a sliding manner; The fixing sleeve is arranged on the base and used for fixing the centrifuge tube; The lower end of the hydraulic rod is provided with a hydraulic suction head for sucking the layered sample to be detected in the centrifuge tube, and the upper end of the hydraulic rod is connected with the sliding table; And the driving device is used for driving the sliding table to lift along the extending direction of the hydraulic support.
3. A detection apparatus for density gradient centrifugation samples as claimed in claim 2, wherein the drive means comprises: a screw mechanism having a screw; And the hydraulic motor is connected with the screw rod through a coupler, and drives the sliding table to drive the hydraulic rod to lift through the screw rod.
4. The detection apparatus for density gradient centrifugation samples as claimed in claim 1, wherein the optical detector mechanism further comprises: and the signal detector is used for collecting ultraviolet spectrum absorption signals of the sample.
5. The detection apparatus for density gradient centrifugation samples as claimed in claim 1, wherein the automated collection platform mechanism comprises: And the collecting needle is used for collecting the sample detected in the sample detection chamber into the sample collecting plate.
6. The detection apparatus for density gradient centrifugation samples as claimed in claim 5, wherein the automated collection platform mechanism further comprises: and the driving system is used for driving the collection needle to move to the area where the sample collection plate is located.
7. The detection apparatus for density gradient centrifugation samples as claimed in claim 6, wherein the driving system comprises an X-axis driving system for driving the collection needle to move in the X-axis direction and/or a Y-axis driving system for driving the collection needle to move in the Y-axis direction.
8. The apparatus according to claim 7, wherein the X-axis driving system drives the Y-axis driving system to move in the X-axis direction, and the collection needle is provided on the Y-axis driving system.
9. The apparatus of claim 1, wherein the automated collection platform mechanism further comprises a waste reservoir.
10. The detection apparatus for density gradient centrifugation samples as claimed in any one of claims 1-9, wherein the automated collection platform mechanism further comprises a heat sink.

Description

Detection device for density gradient centrifugal sample Technical Field The utility model relates to the technical field of detection devices, in particular to a detection device for a density gradient centrifugal sample. Background Density gradient centrifugation is the basic and most commonly used separation means in biochemical and life science research, and uses the difference of sedimentation coefficients of different substances, under the condition of a certain centrifugal speed or centrifugal force, the substances with different sedimentation coefficients are separated to form a strip in the region with different density gradients, and typical applications thereof include separation of different density lipoproteins, purification and separation of large cell particles, separation of nucleic acid, separation of subcellular particles, separation of biological membranes and the like, and common density gradient preparation liquids include sucrose, cesium chloride, sodium bromide and the like. How to collect our target substances quickly, conveniently and accurately from the different density bands formed after density gradient centrifugation is a problem that is still to be solved at present. The three methods of collecting after finishing the density gradient centrifugation are firstly to pierce the liquid surface slowly from above by naked eyes and then to draw the liquid into the target layer manually, secondly to pierce the bottom of the centrifuge tube by a needle and then to collect the liquid, and the flow rate can be controlled by improvement in the later period, but the two methods can lead to particle residues of part of the centrifuge tube wall due to the vertical movement of the gradient, so that the resolution is not high, and the third method is a full-automatic density gradient preparation and separation system of the Biocomp company, which solves the problem of particle residues of the centrifuge tube wall by the movement of a piston from top to bottom, and increases the resolution and accuracy of the collection by installing corresponding LED lamps with the wavelengths of UV 260nm and 280nm, but the current Biocomp company only has two light sources with the wavelengths of 260nm and 280nm, and is very effective for collecting spectral materials with the two characteristic wavelengths, such as nucleic acid and protein, but has larger limitation, and the first is a separation system and a collection system which is two independent systems, and the second method is limited by the selection of two wavelengths, so that the sample signal collection of the two types of particles can not be collected by selecting a large sample, such as a liposome. Disclosure of utility model In view of this, in order to solve the technical problems that in the prior art, the separation system and the collection system of the fully automatic density gradient preparation and separation system are two independent systems and can only realize the selection of two wavelengths, so that the collection of some samples such as liposome, cell macroparticles and the like has no selectable signal collection mode. The utility model provides a detection device for a density gradient centrifugal sample, which reduces human interference and improves resolution and efficiency by integrating a separation system and a collection system into a continuous flow. By adopting the xenon lamp light source and the beam shrinking light path system, the detection precision and stability are improved based on the principle of a spectrophotometer, the detection wavelength can be freely selected, and the limitation of the traditional dual wavelength is broken through. In order to achieve the above purpose, the present utility model provides the following technical solutions: A detection apparatus for density gradient centrifugation samples, comprising: the sample sucking platform mechanism is used for sucking layered samples to be detected in the centrifuge tube layer by layer and conveying the layered samples to a sample detection chamber of the optical detector mechanism; the optical detector mechanism is provided with a xenon lamp light source and a beam shrinking light path system and supports the detection of the full wavelength of 200nm-1100 nm; And the automatic collection platform mechanism is used for sub-packaging the samples detected by the optical detector mechanism. Preferably, the sample aspiration platform mechanism comprises: the base is arranged on a platform of the sample sucking platform mechanism; The hydraulic support is arranged on the base; The sliding table is connected to the hydraulic support in a sliding manner; The fixing sleeve is arranged on the base and used for fixing the centrifuge tube; The lower end of the hydraulic rod is provided with a hydraulic suction head for sucking the layered sample to be detected in the centrifuge tube, and the upper end of the hydraulic rod is connected with the sliding table; A