CN-224203032-U - Multimode microscopic image acquisition device

CN224203032UCN 224203032 UCN224203032 UCN 224203032UCN-224203032-U

Abstract

The utility model discloses a multimode microscopic image acquisition device which comprises a mounting platform, a translation driving mechanism mounted on the mounting platform and a sample cabin assembly connected with the translation driving mechanism, wherein a first through hole is formed in the top of the mounting platform, a light source bright field assembly is further arranged above the first through hole on the top of the mounting platform, a Z-axis lifting mechanism is further mounted at the bottom of the mounting platform, the Z-axis lifting mechanism is further in driving connection with a fluorescence imaging assembly, the fluorescence imaging assembly is arranged below the first through hole, and a sample mixing assembly is further mounted on the mounting platform. The utility model integrates sample processing components such as mixing, temperature regulation and the like, can collect bright field signals, fluorescent signals and absorbance of multiple spectrums at the same time, and provides a basic technical tool for multi-parameter characterization of complex biological samples.

Inventors

WANG ZHIKUN
LIN WEIHUI

Assignees

深圳艾米医疗科技有限公司

Dates

Publication Date: 20260505
Application Date: 20250521

Claims (10)

1. The multi-mode microscopic image acquisition device is characterized by comprising a mounting platform, a translation driving mechanism mounted on the mounting platform and a sample cabin assembly connected with the translation driving mechanism, wherein a first through hole is formed in the top of the mounting platform, a light source bright field assembly is further arranged above the first through hole in the top of the mounting platform, a Z-axis lifting mechanism is further mounted at the bottom of the mounting platform, the Z-axis lifting mechanism is further in driving connection with a fluorescence imaging assembly, the fluorescence imaging assembly is arranged below the first through hole, and a sample mixing assembly is further mounted on the mounting platform.
2. The multimode microscopic image acquisition device according to claim 1, wherein the sample compartment component comprises a sample carrying platform and heating plates, the sample carrying platform comprises a first connecting seat connected with a translation driving mechanism, one ends of two sides of the first connecting seat are respectively connected with a carrying seat in an integrated mode, the two carrying seats are arranged in parallel, one ends of the two carrying seats extend outwards in the direction away from the first connecting seat, the first through hole is located between the two carrying seats, each carrying seat is further provided with a clamping component, the heating plates are arranged at the bottoms of the first connecting seat, one ends of the heating plates are respectively branched, and the two heating portions are respectively correspondingly arranged at the bottoms of one carrying seat.
3. The multimode microscopic image acquisition device according to claim 2, wherein the lower parts of the opposite sides of the two bearing seats are respectively provided with a bearing table, the clamping assembly comprises an elastic clamping piece, the elastic clamping piece is arranged along the length direction of the bearing table, one end of the elastic clamping piece is arranged at the top of the bearing seat, the other end of the elastic clamping piece is bent downwards and extends to the bearing table, and the end part of the elastic clamping piece extending to the bearing table is also bent upwards.
4. The multi-modality microscopic image acquisition device of claim 3, wherein the sample pod assembly further includes a thermostatic cover mounted to the two carrying seats, and wherein a light-transmitting glass is further mounted to the thermostatic cover.
5. The multimode microscopic image acquisition device according to claim 2, wherein the translation driving mechanism comprises a Y-axis translation mechanism installed at the bottom of the installation platform, a Y-axis sliding rail arranged at the top of the installation platform, a Y-axis sliding table slidingly arranged on the Y-axis sliding rail and connected with the Y-axis translation mechanism, and an X-axis translation mechanism installed at the top of the Y-axis sliding table, wherein an X-axis sliding rail is further arranged at the top of the Y-axis sliding table, and the first connecting seat is slidingly arranged on the X-axis sliding rail and connected with the X-axis translation mechanism.
6. The multi-mode microscopic image acquisition device according to claim 1, wherein the top of the mounting platform is further provided with a first bracket, the first bracket is further connected with a mounting cross plate, the light source bright field assembly comprises a white light source assembly arranged on the mounting cross plate and a red-green light source assembly arranged on the white light source assembly, and a code scanner is further arranged on one side of the mounting cross plate.
7. The multi-mode microscopic image acquisition device according to claim 1, wherein a fixed seat is further connected to the bottom of the mounting platform, a Z-axis lifting mechanism is mounted on the fixed seat, the fluorescent imaging assembly comprises a Z-axis sliding table, a fluorescent light source assembly and an imaging assembly, a Z-axis sliding rail is further arranged on one side of the fixed seat along the vertical direction, the Z-axis sliding table is slidingly arranged on the Z-axis sliding rail and is connected with the Z-axis lifting mechanism, the imaging assembly is arranged on one side of the Z-axis sliding table along the vertical direction, and the fluorescent light source assembly is mounted on the imaging assembly.
8. The multimode microscopic image acquisition device according to claim 1, wherein the sample mixing assembly comprises a mixing motor, a connecting cylinder and a mixing magnet, a second through hole is further formed in the position, close to the first through hole, of the top of the mounting platform, the mixing motor is mounted at the bottom of the mounting platform, an output shaft of the mixing motor is inserted into the second through hole and is arranged, the connecting cylinder is mounted on the output shaft of the mixing motor, the outer wall of the connecting cylinder is further connected with the mounting cylinder, the upper portion of the mounting cylinder penetrates out of the second through hole and extends upwards, and the mixing magnet is mounted in the mounting cylinder.
9. The multi-mode microscopic image acquisition device according to claim 1, further comprising a magnetic fixing assembly, wherein the magnetic fixing assembly comprises a fixing clamping seat and a fixing magnet, the fixing clamping seat is detachably arranged on the fluorescent imaging assembly, the top of the fixing clamping seat is further provided with a mounting groove, and the fixing magnet is mounted in the mounting groove.
10. The multimode microscopic image acquisition device according to claim 7, wherein the bottom of the fixing seat is further connected with a supporting frame, and a main control board assembly and a driving board assembly connected with the main control board assembly are further installed on the supporting frame.

Description

Multimode microscopic image acquisition device Technical Field The utility model relates to the technical field of microscopic imaging, in particular to a multi-mode microscopic image acquisition device. Background With the rapid development of life sciences and clinical medicine, the requirement for multi-dimensional characterization of complex samples is difficult to meet by the traditional single-mode microscopic imaging technology. Multiple methodological studies require simultaneous acquisition of multiple physical signals (e.g., fluorescence, multispectral, optical density) while imaging. The existing commercial multispectral microscope system is characterized in that a filter wheel is adopted to realize spectrum light splitting in order to achieve universality and economic benefits. However, such schemes suffer from the significant disadvantage that mechanical filter switching results in limited temporal resolution (typical switching time >50 ms) and the inability to capture fast dynamic processes. Disclosure of utility model The utility model aims to provide a multi-mode microscopic image acquisition device which integrates sample processing components such as mixing, temperature adjustment and the like, can acquire bright field signals, fluorescent signals and absorbance of multiple spectrums at the same time, and provides a basic technical tool for multi-parameter characterization of complex biological samples. In order to achieve the above purpose, the following technical scheme is adopted: The multi-mode microscopic image acquisition device comprises a mounting platform, a translation driving mechanism and a sample cabin assembly, wherein the translation driving mechanism is mounted on the mounting platform, the sample cabin assembly is connected with the translation driving mechanism, a first through hole is formed in the top of the mounting platform, a light source bright field assembly is further arranged above the first through hole in the top of the mounting platform, a Z-axis lifting mechanism is further mounted at the bottom of the mounting platform, the Z-axis lifting mechanism is further in driving connection with a fluorescence imaging assembly, the fluorescence imaging assembly is arranged below the first through hole, and a sample mixing assembly is further mounted on the mounting platform. The sample cabin assembly comprises a sample carrying platform and heating plates, wherein the sample carrying platform comprises a first connecting seat connected with a translation driving mechanism, one ends of two sides of the first connecting seat are respectively and integrally connected with a bearing seat, the two bearing seats are arranged in parallel, one ends of the two bearing seats extend outwards in the direction away from the first connecting seat, the first through holes are arranged between the two bearing seats, each bearing seat is further provided with a clamping assembly, the heating plates are arranged at the bottoms of the first connecting seat, one ends of the heating plates are respectively branched with two heating parts, and the two heating parts respectively extend to the bottoms of one bearing seat correspondingly. Further, the lower parts of the opposite sides of the two bearing seats are respectively provided with a bearing table extending outwards, the clamping assembly comprises elastic clamping pieces, the elastic clamping pieces are arranged along the length direction of the bearing tables, one ends of the elastic clamping pieces are arranged at the tops of the bearing seats, the other ends of the elastic clamping pieces are bent downwards and extend to the bearing tables to be arranged, and the end parts of the elastic clamping pieces extending towards the bearing tables are also bent upwards. Further, the sample cabin assembly further comprises constant temperature covers arranged on the two bearing seats, and light-transmitting glass is further arranged on the constant temperature covers. Further, the translation driving mechanism comprises a Y-axis translation mechanism arranged at the bottom of the mounting platform, a Y-axis sliding rail arranged at the top of the mounting platform, a Y-axis sliding table which is arranged on the Y-axis sliding rail in a sliding manner and connected with the Y-axis translation mechanism, and an X-axis translation mechanism arranged at the top of the Y-axis sliding table, wherein an X-axis sliding rail is further arranged at the top of the Y-axis sliding table, and the first connecting seat is arranged on the X-axis sliding rail in a sliding manner and connected with the X-axis translation mechanism. Furthermore, a first bracket is further arranged at the top of the mounting platform, a mounting transverse plate is further connected to the first bracket, the light source bright field assembly comprises a white light source assembly arranged on the mounting transverse plate and a red-green light source assembly arranged on th