CN-224203041-U - Surface-enhanced Raman micro-fluid detection system for optical fiber optical tweezers

CN224203041UCN 224203041 UCN224203041 UCN 224203041UCN-224203041-U

Abstract

The utility model relates to a fiber-optic tweezers surface enhanced Raman micro-fluid detection system, and belongs to the field of biochemical analysis instruments. The system comprises a fish bone type micro-flow substrate, a microsphere control optical fiber, a Raman excitation light transmission optical fiber, a Raman signal receiving optical fiber, a capturing light source, a Raman excitation light source, a spectrometer, an electric control displacement platform and a control system, wherein the fish bone type micro-flow substrate is provided with a groove for fluid to pass through, the groove is also provided for placing the microsphere control optical fiber, the Raman excitation light transmission optical fiber and the Raman signal receiving optical fiber, the microsphere control optical fiber is arranged on the electric control displacement platform and connected with the capturing light source, the distance between the microspheres can be regulated and controlled, further dynamic adjustment of sensitivity enhancement factors of a detection system is realized, and the front ends of the Raman signal receiving optical fiber and the Raman excitation light transmission optical fiber are immersed in a central detection area of the fish bone type micro-flow substrate, and the rear ends of the Raman signal receiving optical fiber and the Raman excitation light source are respectively connected with the spectrometer and the Raman excitation light source.

Inventors

YU JIAN
Long Huihong
LI SIYING
YANG YUCHUAN
Tong Shandong

Assignees

重庆邮电大学

Dates

Publication Date: 20260505
Application Date: 20250410

Claims (7)

1. The surface-enhanced Raman micro-fluid detection system for the optical fiber tweezers is characterized by comprising a fish bone-shaped micro-fluid substrate, a microsphere control optical fiber I (4), a microsphere control optical fiber II (5), a Raman excitation light transmission optical fiber (6), a Raman signal receiving optical fiber (3), a capturing light source (12), a Raman excitation light source (13), a spectrometer (11), an electric control displacement platform (15) and a control system (14); The fish bone-shaped microfluidic substrate is provided with grooves for fluid to pass through, and grooves for placing a microsphere control optical fiber I (4), a microsphere control optical fiber II (5), a Raman excitation light transmission optical fiber (6) and a Raman signal receiving optical fiber (3); the microsphere control optical fiber I (4) and the microsphere control optical fiber II (5) are arranged on an electric control displacement platform (15), and the tail ends of the microsphere control optical fiber I (4) and the microsphere control optical fiber II (5) are connected with a light source of capturing light (12); The front ends of the Raman signal receiving optical fiber (3) and the Raman excitation light transmission optical fiber (6) are immersed into a central detection area (10) of the fish bone type micro-fluidic substrate, and the rear ends of the Raman signal receiving optical fiber and the Raman excitation light transmission optical fiber are respectively connected with a spectrometer (11) and a Raman excitation light source (13); The spectrometer (11), the light capturing light source (12), the Raman excitation light source (13) and the electric control displacement platform (15) are all connected with the control system (14).
2. The fiber optic tweezers surface enhanced Raman micro-fluidic detection system according to claim 1, wherein the "fishbone" micro-fluidic substrate comprises a main "backbone" groove (i) and eight side branch "fishbone" grooves (a, b, g, h, c, d, e, f), wherein four side branch "fishbone" grooves (a, b, g, h) and one main "backbone" groove (i) are grooves for fluid to pass through, and four side branch "fishbone" grooves (c, d, e, f) are grooves for placing functional optical fibers.
3. The surface-enhanced raman microfluidic detection system of claim 1 or 2, wherein the "fishbone" microfluidic substrate further comprises a microfluidic channel inlet I (1) and a microfluidic channel inlet II (2) through which a fluid to be detected is injected into the detection system.
4. The surface enhanced raman micro-fluidic detection system of claim 1 or 2, wherein the "fishbone" type micro-fluidic substrate further comprises a micro-fluidic channel outlet I (7) and a micro-fluidic channel outlet II (8).
5. The surface-enhanced raman micro-fluidic detection system of claim 4, wherein the "fishbone" type micro-fluidic substrate is provided with a channel switch (9) at the intersection of the micro-fluidic channel outlet I (7) and the micro-fluidic channel outlet II (8), and is connected to a control system (14) for controlling the fluid output channel.
6. The surface enhanced raman micro-fluidic detection system of fiber optic tweezers according to claim 5, wherein the user is further enabled to implement sorting of fluids by control of the channel switch (9) by the control system (14).
7. The surface-enhanced raman micro-fluidic detection system of fiber optical tweezers according to claim 1, wherein when the control system (14) receives the signal from the spectrometer (11), the control system sends the signal to the electric control displacement platform (15) and the capturing light source (12) according to the user's need, and under the combined action of the two, the positions of 2 coated metal particle microspheres (101) are regulated and controlled through the microsphere control fibers I, II (4, 5).

Description

Surface-enhanced Raman micro-fluid detection system for optical fiber optical tweezers Technical Field The utility model belongs to the field of biochemical analysis instruments, relates to the fields of Raman spectroscopy, particle light control and biomedicine, and in particular relates to a surface-enhanced Raman micro-fluid detection system of optical fiber optical tweezers. Background Raman spectroscopy is a spectroscopic technique that uses the phenomenon of optical raman scattering to study molecular vibrations, and can reveal microscopic information such as the morphology, molecular structure, phase, chemical composition, and crystal state of a sample. At present, compared with other detection technologies, the Raman spectrum technology has the characteristics of non-destructive property, rapidness, simple sample preparation, rich information and the like, and is widely applied to various fields such as biomedicine, material science, chemical analysis, artistic archaeology and the like. The Surface Enhanced Raman Spectroscopy (SERS) technology not only inherits the advantages of conventional raman spectroscopy detection. In addition, SERS enhances the raman signal of molecules adsorbed on metal nanostructures 10 4 to 10 15 times by a localized surface-bulk resonance electric field enhancement (LSPR) mechanism. The high sensitivity enables SERS to detect molecules with extremely low concentration, and is widely applied to the fields of trace analysis, biomedical detection, environmental monitoring and the like. However, the existing SERS technology still faces three major core challenges that ① substrate preparation technology is complex, signal reproducibility is poor due to insufficient control accuracy of nanostructure uniformity, ② 'hot spot' regions are randomly spatially distributed, target molecules are difficult to accurately locate to an enhanced region, and the problem of biocompatibility of ③ metal nanostructures and detection environments restricts in-vivo application of the target molecules. Advances in optical manipulation technology provide new solutions to the above-mentioned bottlenecks. Optical tweezers technology is a typical representation of the photodynamic effect, whose physical basis is derived from the principle of gradient force and scattering force balance proposed by Arthur Ashkin in 1986. When the focused laser beam acts on the dielectric particles, an optical potential well of the order of Buffalo (10 -12 N) can be generated, and three-dimensional non-contact control of micro-nano particles is realized. The modern optical tweezers system has developed various forms such as holographic optical tweezers, optical fiber optical tweezers and the like, and is widely applied to the fields of single cell sorting, DNA mechanical property measurement and the like. However, the integration of the traditional optical tweezers technology and a spectrum detection system has obvious technical barriers that ① high-power capture laser causes the risk of sample thermal injury, the compatibility with fragile biological samples is poor, the space coupling precision of a ② optical tweezers optical path and a detection optical path is required to reach submicron level, the system is huge due to the multi-module discrete design, the space pose of particles is randomly changed in the ③ dynamic capture process, and stable and reliable in-situ spectrum acquisition is difficult to realize. The prior art has two major trends, namely, on one hand, SERS substrate preparation is developing towards the controllable and functional directions, for example, a periodic nano array prepared by adopting electron beam lithography can improve signal uniformity but has high manufacturing cost, and on the other hand, a light power-spectrum combination technology becomes a front-edge hot spot, for example, an optical tweezers-SERS probe developed by Harvard university team can acquire Raman fingerprints of single virus particles while capturing the single virus particles, but the prior proposal needs to alternately switch capturing and detecting lasers, and signal loss is caused by time sequence control errors. How to realize the in-situ synchronous detection of the optical power control and the enhanced spectrum and break through the limit of the spatial resolution and the detection sensitivity in the prior art becomes a core technical problem to be overcome in the field. Disclosure of utility model In view of the above, the utility model aims to provide a novel optical fiber optical tweezers surface enhanced Raman micro-fluid detection system, which aims at solving the defects of low sensitivity, lack of flexibility, complex detection system, poor LSPR hot spot enhancement controllability, poor repeatability and the like of the existing Raman detection system. In addition, compared with the traditional Raman detection system, the system provided by the utility model has the advantages that the system complexity