CN-224203212-U - Enzyme-labeled instrument equipped with automatic sample-adding and inactivating module

CN224203212UCN 224203212 UCN224203212 UCN 224203212UCN-224203212-U

Abstract

The utility model relates to an enzyme-labeled instrument provided with an automatic sample adding and inactivating module, which comprises a rack, a pipetting module, a detection module, an inactivating module, a plurality of independent inactivating units, a sample adding device and a sample holder, wherein the inactivating module comprises an inactivating cavity, a multichannel sample adding head and a rotating component, the inactivating cavity is internally provided with the plurality of independent inactivating units, each inactivating unit comprises a heating plate, a cooling plate and a temperature control sensor, the multichannel sample adding head is connected with the top of the inactivating cavity through a lifting rod and comprises a plurality of sample adding channels, each sample adding channel is provided with a micropump and a flow controller, the bottom of each sample adding channel is provided with a nozzle, the rotating component comprises a rotating shaft and a plurality of fixing arms, and the rotating shaft penetrates through the top of the inactivating cavity and is in transmission connection with a motor, and the tail end of each fixing arm is provided with the sample holder. The enzyme-labeled instrument with the automatic sample adding and inactivating module can realize automatic sample adding, accurate inactivation and multi-stage treatment of samples, remarkably improve the inactivation efficiency and the automation degree, and enhance the universality and the compatibility of equipment.

Inventors

LU HAO
Ouyang jiayi
QUAN MINGXIA
LI ZHAOFENG
LI DANYANG

Assignees

江南大学

Dates

Publication Date: 20260505
Application Date: 20250522

Claims (10)

1. An enzyme-labeled instrument equipped with an automatic sample-adding and inactivating module, comprising: The top of the rack is provided with a sample tray which is round and can rotate around a central shaft; the pipetting module is positioned at one side of the sample tray and is connected with the rack through a sliding rail; The detection module is fixed in the middle of the rack and positioned below the sample tray; The inactivation module comprises an inactivation cavity, a multichannel sample adding head and rotating components, wherein a plurality of independent inactivation units are arranged in the inactivation cavity, each inactivation unit comprises a heating plate, a cooling plate and a temperature control sensor, the heating plates are fixed on the upper part of the inner wall of the inactivation cavity, the cooling plates are fixed on the lower part of the inner wall of the inactivation cavity, the temperature control sensors are embedded in the side wall of the inactivation cavity and are electrically connected with the heating plates and the cooling plates, the multichannel sample adding head is connected with the top of the inactivation cavity through lifting rods, the multichannel sample adding head comprises a plurality of sample adding channels, each sample adding channel is provided with a micropump and a flow controller, the bottom of each sample adding channel is provided with a nozzle, each rotating component comprises a rotating shaft and a plurality of fixing arms, the rotating shaft penetrates through the top of the inactivation cavity and is in transmission connection with a motor, the fixing arms are radially distributed on the outer wall of the rotating shaft, and the tail end of each fixing arm is provided with a sample holder.
2. The enzyme-labeled instrument equipped with the automatic sample adding and inactivating module according to claim 1, wherein the top of the inactivating cavity is provided with a sample inlet and a sample outlet, the sample inlet and the sample outlet are respectively positioned at two sides of the inactivating cavity, and electromagnetic valves are respectively arranged at the sample inlet and the sample outlet.
3. The enzyme label instrument with the automatic sample adding and inactivating module according to claim 1, wherein a gear set is arranged between the multichannel sample adding head and the lifting rod, threads are arranged on the outer wall of the lifting rod, the threads are meshed with the gear set, and the gear set is in transmission connection with the stepping motor.
4. The enzyme label instrument equipped with the automatic sample adding and inactivating module according to claim 1, wherein the inner wall of the sample holder is provided with an anti-slip pad, and protrusions are uniformly distributed on the surface of the anti-slip pad.
5. The enzyme label instrument with the automatic sample adding and inactivating module according to claim 1, wherein the heating plate and the cooling plate are made of metal.
6. The microplate reader equipped with an automatic sample addition and inactivation module according to claim 1, wherein the number of the sample addition channels is four to eight.
7. The microplate reader equipped with an automatic sample application and inactivation module according to claim 4, wherein an elastic member is disposed between the sample holder and the fixed arm, and the sample holder is connected to the fixed arm via the elastic member.
8. The enzyme label instrument with the automatic sample adding and inactivating module according to claim 1, wherein the number of the fixing arms is four to six, and the fixing arms are identical in length and distributed on the outer wall of the rotating shaft in an annular array mode.
9. The microplate reader equipped with an automatic sample application and inactivation module according to claim 1, wherein the motor is connected with the rotating shaft through a belt or gear transmission system.
10. The enzyme label instrument with the automatic sample adding and inactivating module according to claim 1, wherein the outer wall of the nozzle is sleeved with a sealing ring, and the nozzle is tightly connected with the bottom of the sample adding channel through the sealing ring.

Description

Enzyme-labeled instrument equipped with automatic sample-adding and inactivating module Technical Field The utility model relates to the technical field of enzyme-labeled instruments, in particular to an enzyme-labeled instrument provided with an automatic sample adding and inactivating module. Background The enzyme-labeled instrument is used as core equipment for biochemical detection, and the application scene of the enzyme-labeled instrument is obviously expanded along with the rapid development of the field of bioengineering. In recent years, breakthrough advances in synthetic biology, metabolic engineering, and protein engineering have driven the exponential growth in demand for high-throughput, automated detection equipment. In practical application, especially in experiments involving pathogenic microorganisms or high-activity biological samples, how to realize automatic sample loading and inactivation integrated operation becomes a key problem for improving detection efficiency and guaranteeing operation safety. In the search, patent document CN112585475B discloses a biochemical cartridge and an analysis device, in which a plurality of electrodes are arranged on a flow path to realize sample transport, and an opening is provided for sampling a biological sample. The design utilizes an electric field to drive liquid drops to move, so that the controllability and the flexibility of the microfluidic system are improved to a certain extent. However, the technology mainly relies on an external high-voltage power supply to control the movement of liquid drops, so that the problems of high energy consumption, high system complexity and the like exist, and in addition, the maintenance cost is high under the conditions of frequent sample replacement or multi-batch continuous detection because the electrodes are easy to pollute and difficult to clean and reuse. Another related art is a biochemical analysis device of publication No. CN112986600B, which detects a change in the height of a nozzle contacting a sample using a liquid level sensor and judges whether an abnormal condition exists based on the detected change. The method can effectively identify the fault state (such as nozzle blockage or collision) in the sample adding process, so that the running stability of the equipment is improved. The device does not relate to the design of a sample inactivation function, can not meet the application requirements of the whole process from sampling to processing in a closed environment, and meanwhile, a control system of the device only carries out response mechanism setting aiming at single type errors, and lacks an integrated management strategy to coordinate the linkage relation between automatic sample adding and subsequent inactivation steps. The prior art has a certain progress in the aspects of micro liquid control and process monitoring, but the core problems that a general automatic sample adding module suitable for various sample types is lacking, a functional unit capable of realizing efficient inactivation treatment on the same platform is lacking, and the whole structure is complex, the manufacturing cost is high, and the large-scale popularization and application are not facilitated are still solved. Disclosure of utility model Therefore, the enzyme-labeled instrument provided by the utility model has the characteristics of being provided with an automatic sample adding and inactivating module, can simplify the flow steps and reduce the cost while ensuring the operation safety, and can enhance the intelligent level of equipment so as to meet the requirements of diversified scientific research and clinical inspection occasions. The utility model provides an enzyme label instrument with an automatic sample adding and inactivating module, which comprises a rack, a pipetting module, a detection module, an inactivating module and a transmission device, wherein the rack is provided with a sample tray at the top, the sample tray is circular and can rotate around a central shaft, the pipetting module is positioned at one side of the sample tray and is connected with the rack through a sliding rail, the detection module is fixed in the middle of the rack and positioned below the sample tray, the inactivating module comprises an inactivating cavity, a multichannel sample adding head and a rotating component, a plurality of independent inactivating units are arranged in the inactivating cavity, each inactivating unit comprises a heating plate, a cooling plate and a temperature control sensor, the heating plate is fixed on the upper part of the inner wall of the inactivating cavity, the cooling plate is fixed on the lower part of the inner wall of the inactivating cavity, the temperature control sensor is embedded in the side wall of the inactivating cavity and is electrically connected with the heating plate and the cooling plate, the multichannel sample adding head is connected with the top of the inactivatin