CN-224203507-U - Multiband microscope lighting device based on digital micromirror device

Abstract

The utility model relates to the technical field of optical devices, and provides a multiband microscope lighting device based on a digital micro-mirror device, which comprises a shell component and a microscope lighting component, wherein the microscope lighting component is arranged in the shell component and comprises a mounting base body, a light source component, a reflecting mirror component and the digital micro-mirror device, the light source component is used for emitting light beams with different wave bands, the reflecting mirror component is positioned on a transmission light path of the light beams, the reflecting mirror component is used for reflecting the light beams, the digital micro-mirror device is arranged on the mounting base body and corresponds to the position of an emission port, and the digital micro-mirror device is used for receiving the light beams reflected by the reflecting mirror component and modulating the intensity, the direction and the pattern of the light beams and is used for emitting the modulated light beams from the emission port. The utility model uses the digital micro-mirror device as a core, integrates the light source components capable of emitting a plurality of light beams with different wave bands, can selectively irradiate a target area, reduces fluorescence bleaching and phototoxicity of non-target areas, and further improves the signal to noise ratio of imaging.

Inventors

• WANG WENJUAN
• CHEN CHUNLAI

Assignees

• 清华大学

Dates

Publication Date

20260505

Application Date

20250516

Claims (10)

1. 1. A multi-band microscope illumination device based on a digital micromirror device, comprising: The shell assembly is provided with an entrance port and an exit port; A microscope illumination assembly comprising: the mounting base body is arranged in the shell assembly; The light source assembly is arranged on the mounting base body and penetrates through the entrance port, and is connected with the case and used for emitting a plurality of light beams with different wave bands; The reflecting mirror assembly is arranged on the mounting base body and positioned on a transmission light path of the light beam, and is used for reflecting the light beam; And the digital micro-mirror device is arranged on the mounting base body and corresponds to the position of the emission port, and is used for receiving the light beam reflected by the reflecting mirror assembly, modulating the intensity, the direction and the pattern of the light beam and emitting the modulated light beam from the emission port.
2. 2. The digital micromirror device-based multiband microscope illumination device according to claim 1, wherein the light source assembly comprises: The fixed base is arranged on the mounting base body; The light source main body is arranged on the fixed base and penetrates through the entrance port, and is connected with the case and used for emitting a plurality of light beams with different wave bands; The adjustable mechanical diaphragm is arranged on the fixed base and positioned at the light emitting end of the light source main body and is used for adjusting the size of the light beam; The aspheric condensing lens is arranged on the fixed base and is positioned on one side of the adjustable mechanical diaphragm, which is away from the light source main body, and the aspheric condensing lens is used for converging light beams.
3. 3. The digital micromirror device-based multiband microscope illumination device according to claim 1, wherein the mirror assembly comprises: The adjusting component is arranged on the mounting base body; the reflecting mirror main body is obliquely arranged on the surface of the adjusting component and is used for reflecting the light beam; The adjusting component is used for adjusting the angle of the reflecting mirror main body and keeping the angle of the reflecting mirror main body low in drift.
4. 4. The digital micromirror device-based multiband microscope illumination device according to claim 3, wherein the adjustment assembly comprises: The adjusting mirror bracket is arranged on the mounting base body and is suitable for adjusting the position along the mounting base body; The supporting main body is arranged on the adjusting mirror bracket and is provided with a supporting inclined plane; wherein, the reflector main body is fixedly arranged on the supporting inclined plane.
5. 5. The digital micromirror device-based multiband microscope illumination device according to claim 1, wherein the digital micromirror device comprises: The circuit layer is arranged on the mounting substrate; The micro mirror unit is rotationally connected with the circuit layer through a torsion hinge and is used for modulating the intensity, the direction and the pattern of the light beam; The micro mirror unit comprises an electrode and an elastic piece, wherein the electrode and the elastic piece are respectively arranged at two sides of the micro mirror unit, the electrode is used for controlling the micro mirror unit to rotate under the action of static electricity, and the elastic piece is used for recovering the micro mirror unit to an initial position when power is cut off.
6. 6. The digital micromirror device based multiband microscope illumination device according to claim 1, wherein the microscope illumination assembly further comprises: And the lens component is arranged on the mounting base body and is opposite to the digital micro-mirror device, and the lens component is used for converging the light beams modulated by the digital micro-mirror device.
7. 7. The digital micromirror device based multiband microscope illumination device according to claim 6, wherein the lens assembly comprises: A fixed carrier arranged on the mounting substrate; And the plano-convex lens is arranged on the fixed carrier and is used for converging the light beams modulated by the digital micromirror device.
8. 8. The illumination device for a multiband microscope based on a digital micro-mirror device according to claim 1, wherein the light source assembly is connected to the housing through a liquid light guide transmission, and the light source assembly is used for emitting six independent light beams, and the switching speed between the channels is 0.1ms.
9. 9. The digital micromirror device based multi-band microscope illumination device according to any one of claims 1 to 8, wherein the housing assembly comprises: a first housing; the second shell is arranged opposite to the first shell, and a heat dissipation hole is formed in the second shell; The light source component and the reflecting mirror component are arranged in the first shell, and the digital micro-mirror device is arranged in the second shell.
10. 10. The digital micromirror device based multiband microscope illumination device according to claim 9, wherein the mounting base comprises: A first substrate; The second base body is connected with the first base body, is arranged perpendicular to the first base body, and has a diagonal line parallel to the central line of the first base body; The digital micro-mirror device is arranged on the second base body and is positioned at the mounting port, and the first shell and the second shell are both abutted against the positioning flange and detachably connected with the second base body.

Description

Multiband microscope lighting device based on digital micromirror device Technical Field The utility model relates to the technical field of optical devices, in particular to a multiband microscope illumination device based on a digital micromirror device. Background Fluorescence imaging occupies a central position in modern biology and medical research, and has wide application in a plurality of fields such as cell biology, molecular biology, drug screening, disease diagnosis and the like. This technique achieves high resolution and visual observations of cellular structure, molecular dynamics, and biological processes by means of fluorescent markers, for example using fluorescent proteins (like GFP, mCherry) or dyes (like DRAQ 5). However, in some specific high-throughput biological experiments, such as CRISPR-based gene function screening, fluorescence imaging has particular needs. In studying cell phenotypes (such as nuclear size, protein localization), it is desirable to excite multiple fluorescent markers rapidly and accurately, and to make selective observations of specific regions. Conventional lighting devices exhibit significant drawbacks in this scenario: For example, the conventional illumination device irradiates the whole visual field indifferently, the illumination pattern is fixed, and the selective excitation of the target area cannot be realized, so that the fluorescent bleaching phenomenon occurs, and meanwhile, the background noise is increased, so that the imaging quality is influenced. Disclosure of utility model The utility model provides a multiband microscope lighting device based on a digital micro-mirror device, which is used for solving the technical defects in the prior art, and integrates a light source assembly capable of emitting light beams with different wavebands by taking the digital micro-mirror device as a core, so that a target area can be selectively irradiated, fluorescence bleaching and phototoxicity of non-target areas are reduced, and further, the signal to noise ratio of imaging is improved. The utility model provides a multiband microscope illumination device based on a digital micromirror device, comprising: The shell assembly is provided with an entrance port and an exit port; A microscope illumination assembly disposed within the housing assembly, the microscope illumination assembly comprising: a mounting substrate; The light source assembly is arranged on the mounting base body and penetrates through the entrance port, and is connected with the case and used for emitting a plurality of light beams with different wave bands; The reflecting mirror assembly is arranged on the mounting base body and positioned on a transmission light path of the light beam, and is used for reflecting the light beam; And the digital micro-mirror device is arranged on the mounting base body and corresponds to the position of the emission port, and is used for receiving the light beam reflected by the reflecting mirror assembly, modulating the intensity, the direction and the pattern of the light beam and emitting the modulated light beam from the emission port. According to the multiband microscope illumination device based on the digital micromirror device provided by the utility model, the light source assembly comprises: The fixed base is arranged on the mounting base body; The light source main body is arranged on the fixed base and penetrates through the entrance port, and is connected with the case and used for emitting a plurality of light beams with different wave bands; The adjustable mechanical diaphragm is arranged on the fixed base and positioned at the light emitting end of the light source main body and is used for adjusting the size of the light beam; The aspheric condensing lens is arranged on the fixed base and is positioned on one side of the adjustable mechanical diaphragm, which is away from the light source main body, and the aspheric condensing lens is used for converging light beams. According to the present utility model, there is provided a digital micromirror device-based multi-band microscope illumination apparatus, the mirror assembly comprising: The adjusting component is arranged on the mounting base body; the reflecting mirror main body is obliquely arranged on the surface of the adjusting component and is used for reflecting the light beam; The adjusting component is used for adjusting the angle of the reflecting mirror main body and keeping the angle of the reflecting mirror main body low in drift. According to the present utility model, there is provided a digital micromirror device-based multi-band microscope illumination apparatus, the adjustment assembly comprising: The adjusting mirror bracket is arranged on the mounting base body and is suitable for adjusting the position along the mounting base body; The supporting main body is arranged on the adjusting mirror bracket and is provided with a supporting inclined plane; wherein, the reflector