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CN-224207443-U - Polypeptide extraction element

CN224207443UCN 224207443 UCN224207443 UCN 224207443UCN-224207443-U

Abstract

The utility model relates to the technical field of polypeptide extraction, and discloses a polypeptide extraction device, which comprises an extractor body, wherein a feed inlet is formed in the top surface of the extractor body, a limiting ring attached to the outer side is arranged on the top surface of the extractor body, an inlet closing component for covering the feed inlet is arranged in the limiting ring, and a detachable raw material freezing and thawing component is arranged on the limiting ring. Through setting up raw materials freeze thawing subassembly in feed inlet department, can carry out freeze thawing to polypeptide raw materials and directly send into the extractor body and carry out the extraction, avoid appearing the pollution phenomenon, but the raw materials freeze thawing subassembly of quick detach makes things convenient for daily maintenance.

Inventors

  • MA WENJIE
  • WANG WENHAN
  • YU JUN

Assignees

  • 上海博腾智拓医药科技有限公司

Dates

Publication Date
20260508
Application Date
20250430

Claims (9)

  1. 1. The utility model provides a polypeptide extraction element, includes extractor body (1), and extractor body (1) top surface is provided with feed inlet (101), its characterized in that, extractor body (1) top surface is provided with spacing ring (102) in the laminating outside, is provided with in spacing ring (102) and covers entry closure assembly (2) of feed inlet (101), is provided with detachable raw materials freeze thawing assembly (3) on spacing ring (102).
  2. 2. The polypeptide extraction device according to claim 1, wherein the inlet closing assembly (2) comprises electric telescopic rods (201) arranged on two sides of the inner wall of the limiting ring (102), the tail ends of the electric telescopic rods (201) are connected with closing plates (202), the symmetrically arranged closing plates (202) are driven by the electric telescopic rods (201) to stretch out and draw back, the symmetrically arranged closing plates are mutually attached to cover the feed inlet (101), and limiting strips (103) attached to two sides of the closing plates (202) are arranged on the top surface of the extractor body (1).
  3. 3. The polypeptide extraction device according to claim 2, wherein the raw material freeze-thawing assembly (3) comprises a freeze-thawing shell (301) which is arranged on the top surface of the limiting ring (102) in a fitting manner, a limiting block (302) which is arranged on the outer side of the limiting strip (103) is arranged on the bottom surface of the freeze-thawing shell (301), a blanking valve pipe (303) is downwards arranged in the middle of the bottom surface of the freeze-thawing shell (301), the blanking valve pipe (303) is arranged in the feeding port (101), a freeze-thawing bin (304) which is fixedly connected with the blanking valve pipe (303) is arranged in the freeze-thawing shell (301), the lower end of the freeze-thawing bin (304) is in a round table shape, the upper end of the freeze-thawing bin (304) is in a cylindrical shape, a heating table (305) which is arranged on the outer side of the lower end of the freeze-thawing bin (304), a cooling annular plate (306) which is in a fitting manner is arranged on the outer side of the upper end of the freeze-thawing bin (304), temperature sensors (307) are arranged on the inner walls, the temperature sensors (307) are arranged on the inner walls, six groups of the inner walls of the freeze-thawing bin (304) are evenly arranged, the top surfaces are arranged on the inner walls, the side of the freeze-thawing bin (304) are connected with the freeze-thawing port (308), and the freeze-thawing port (308) is arranged on the side, and the side of the freeze-thawing assembly.
  4. 4. A polypeptide extraction device according to claim 3, characterized in that the top surface of the freeze-thaw housing (301) is provided with viewing windows (310) at symmetrical sides of the addition port (308).
  5. 5. The polypeptide extraction device according to claim 3 or 4, wherein the bottom surface of the freeze-thawing casing (301) is symmetrically provided with fixing blocks (311) attached to the inner wall of the limiting ring (102), and the fixing blocks (311) are fixedly connected with the limiting ring (102) through bolts.
  6. 6. The polypeptide extraction device according to claim 3 or 4, wherein a stirring motor (312) is arranged in the middle of the top surface of the freeze thawing shell (301), a driving shaft of the stirring motor (312) is connected with a stirring rod (313), the stirring rod (313) is arranged in the freeze thawing chamber (304), an arc stirring blade (314) is arranged on the stirring rod (313) positioned at the upper end of the freeze thawing chamber (304), and a scraping stirring blade (315) attached to the inner wall of the freeze thawing chamber (304) is arranged on the stirring rod (313) positioned at the lower end of the freeze thawing chamber (304).
  7. 7. The polypeptide extraction device according to claim 5, wherein a stirring motor (312) is arranged in the middle of the top surface of the freeze thawing shell (301), a driving shaft of the stirring motor (312) is connected with a stirring rod (313), the stirring rod (313) is arranged in the freeze thawing chamber (304), an arc stirring blade (314) is arranged on the stirring rod (313) positioned at the upper end of the freeze thawing chamber (304), and a scraping plate stirring blade (315) attached to the inner wall of the freeze thawing chamber (304) is arranged on the stirring rod (313) positioned at the lower end of the freeze thawing chamber (304).
  8. 8. The polypeptide extraction device according to claim 6, wherein control boxes (316) are arranged on two sides of the top surface of the freeze thawing shell (301), a pressing stirrer (317) attached to the inner wall of the upper end of the freeze thawing bin (304) is arranged on the bottom surface of each control box (316), and the pressing stirrer (317) is kept in non-contact with the arc-shaped stirring blades (314).
  9. 9. The polypeptide extraction device according to claim 7, wherein control boxes (316) are arranged on two sides of the top surface of the freeze thawing shell (301), a pressing stirrer (317) attached to the inner wall of the upper end of the freeze thawing bin (304) is arranged on the bottom surface of each control box (316), and the pressing stirrer (317) is kept in non-contact with the arc-shaped stirring blades (314).

Description

Polypeptide extraction element Technical Field The utility model relates to the technical field of polypeptide extraction, in particular to a polypeptide extraction device. Background The polypeptide is a compound formed by connecting 2-50 amino acids through peptide bonds, and is widely involved in physiological processes such as signal transmission, immunoregulation, enzyme catalysis and the like of organisms. The polypeptide extraction is mainly realized by a natural extraction method, a chemical synthesis method and a gene recombination method. The extraction process requires strict control of temperature, such as cell disruption at low temperature, enzymolysis at proper temperature or synthesis, so as to avoid polypeptide denaturation, and the purification and activity are ensured by combining chromatographic separation, freeze-drying and other technologies, and the method is finally applied to the fields of medicines, foods, cosmetics and the like. In the polypeptide extraction stage, freeze thawing treatment is needed to be carried out on the extracted sample, namely, the cells are repeatedly frozen and thawed for a plurality of times within the temperature range of-20 ℃ to 37 ℃ so that the cell membrane structure is destroyed, intracellular polypeptides are released, and polypeptide denaturation caused by high temperature is avoided. The existing polypeptide extraction can separate the freeze thawing treatment process from the extraction process, and transfer the polypeptide by other tools after the freeze thawing treatment is finished, the operation is complex, samples can be exposed to room temperature or high temperature in the transfer process, so that the polypeptide is denatured or degraded, and the samples can be contacted with environmental microorganisms or impurities, so that the purity of the polypeptide is reduced due to the introduction of pollution. Disclosure of utility model Aiming at the defects of the prior art, the utility model provides a polypeptide extraction device which can be used for carrying out freeze thawing treatment on polypeptide raw materials and directly sending the polypeptide raw materials into an extractor body for extraction, so that the pollution phenomenon is avoided. In order to achieve the purpose, the technical scheme is that the polypeptide extraction device comprises an extractor body, a feed inlet is formed in the top surface of the extractor body, a limiting ring attached to the outer side is arranged on the top surface of the extractor body, an inlet closing component for covering the feed inlet is arranged in the limiting ring, and a detachable raw material freezing and thawing component is arranged on the limiting ring. Further, the inlet closing assembly comprises electric telescopic rods arranged on two sides of the inner wall of the limiting ring, the tail ends of the electric telescopic rods are connected with closing plates, the symmetrically arranged closing plates stretch under the driving of the electric telescopic rods, the closing plates are mutually attached to cover the feeding hole in a closing mode, and limiting strips attached to two sides of the closing plates are arranged on the top surface of the extractor body. Further, the raw materials freeze thawing subassembly is including the freeze thawing shell that the laminating set up at the spacing top surface, freeze thawing shell bottom surface is provided with the stopper of laminating in spacing outside, be provided with the unloading valve pipe downwards in the middle of the freeze thawing shell bottom surface, the unloading valve pipe is placed in the feed inlet, be provided with in the freeze thawing shell with unloading valve pipe fixed connection's freeze thawing storehouse, freeze thawing storehouse lower extreme is round platform shape and upper end is cylindrically, freeze thawing storehouse lower extreme outside is provided with the heating bench of laminating, freeze thawing storehouse upper end outside is provided with the cooling annular slab of laminating, freeze thawing storehouse upper end and lower extreme all are provided with temperature sensor on the inner wall, temperature sensor evenly is provided with six groups on freeze thawing storehouse inner wall circumference, freeze thawing shell top surface one side is provided with the interpolation mouth that leads to freeze thawing storehouse, the interpolation mouth side is provided with the temperature display panel who connects temperature sensor. Further, the top surface of the freeze-thawing shell is provided with observation windows on symmetrical sides of the adding port. Further, fixed blocks attached to the inner wall of the limiting ring are symmetrically arranged on the bottom surface of the freeze-thawing shell, and the fixed blocks are fixedly connected with the limiting ring through bolts. Further, be provided with agitator motor in the middle of the freeze thawing shell top surface, agitator motor's dri