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CN-224208049-U - Microfluidic chip

CN224208049UCN 224208049 UCN224208049 UCN 224208049UCN-224208049-U

Abstract

The utility model discloses a microfluidic chip, which belongs to the technical field of medical instruments, and comprises an upper cover and a separation container, wherein the upper cover is arranged on the separation container, the top of the separation container is provided with a sealing layer, the bottom of the upper cover is abutted with the sealing layer, the upper cover and the sealing layer are enclosed to form an inner cavity, a separation chip set is arranged in the inner cavity, the top of the separation chip set is abutted with the top of the upper cover, the bottom of the separation chip set is abutted with the sealing layer, the separation chip set comprises a plurality of layers of separation chips, and the separation chips are used for separating blood cells in blood from enriched PRP plasma. Solves the problem that the boundary layer position is difficult to judge and control caused by the thinner thickness of the boundary layer between the blood plasma and the red blood cells after blood centrifugation and delamination, and finally prepares the platelet-rich blood plasma with stable PRP enrichment multiple, more PRP collection quantity and less red blood cell residue.

Inventors

  • SUN SHOUYU
  • SHEN YANZHEN
  • YOU ZIYANG
  • TIAN XIAOLEI

Assignees

  • 山东同齐医学科技有限公司

Dates

Publication Date
20260508
Application Date
20250417

Claims (6)

  1. 1. The microfluidic chip comprises an upper cover (1) and a separation container (2), wherein the upper cover (1) is arranged on the separation container (2), and is characterized in that an encapsulation layer (202) is arranged at the top of the separation container (2), the bottom of the upper cover (1) is abutted against the encapsulation layer (202), an inner cavity is formed by encircling the upper cover (1) and the encapsulation layer (202), a separation chip set is arranged in the inner cavity, the top of the separation chip set is abutted against the top of the upper cover (1), the bottom of the separation chip set is abutted against the encapsulation layer (202), the separation chip set comprises a plurality of layers of separation chips (3), and the separation chips (3) are used for separating blood cells in blood from enriched PRP plasma.
  2. 2. The microfluidic chip of claim 1, wherein: The separation chip (3) comprises a blood inlet (301), a blood channel (302) which is arranged in a mirror image mode, a plurality of PRP outlets (307) and a blood cell outlet (308) which is arranged in the mirror image mode, one end of the blood channel (302) is communicated with the blood inlet (301), and the other end of the blood channel (302) is communicated with the blood cell outlet (308).
  3. 3. The microfluidic chip of claim 2, wherein: Each blood channel (302) is provided with a contraction section (303), a throat (304) and an expansion section (305) which are communicated in sequence, one end of the expansion section (305) is communicated with the throat (304), and the other end of the expansion section (305) is communicated with a blood cell outlet (308); The expanding section (305) is provided with a micro-channel (306) near the throat (304) in a mirror image way, one end of the micro-channel (306) is communicated with the expanding section (305), and the other end of the micro-channel (306) is communicated with the PRP outlet (307).
  4. 4. A microfluidic chip according to claim 3, wherein: a plurality of PRP inlets (203) and blood cell inlets (204) which are arranged in a mirror image mode are arranged on the seal layer (202), the PRP inlets (203) are aligned with the PRP outlets (307), and the blood cell inlets (204) are aligned with the blood cell outlets (308).
  5. 5. The microfluidic chip according to claim 4, wherein: The separation container (2) comprises a PRP collecting area (205) and a blood cell collecting area (207), wherein the PRP inlet (203) is communicated with the PRP collecting area (205), and the blood cell inlet (204) is communicated with the blood cell collecting area (207); The side wall of the separating container (2) at the PRP collecting zone (205) is provided with an extraction port (201).
  6. 6. The microfluidic chip of claim 5, wherein: an upper cover injection opening (101) is arranged at the top of the upper cover (1), and the upper cover injection opening (101) is aligned with the blood inlet (301).

Description

Microfluidic chip Technical Field The utility model relates to a microfluidic chip, and belongs to the technical field of medical appliances. Background Platelet Rich Plasma (PRP), which is a platelet concentrate obtained after centrifugation of autologous whole blood, has a platelet concentration at least 2 times higher than the baseline concentration. Since autologous whole blood is mostly adopted in the preparation of PRP at present, PRP is generally referred to as autologous PRP and mainly contains components such as platelets, fibrin, white blood cells and the like. Alpha particles in the platelets release a large amount of growth factors after the platelets are activated, and the proportion of the growth factors is consistent with the proportion of physiological states in the body, so that the alpha particles play a very important role in accelerating wound healing, promoting tissue regeneration and repair, relieving pain and the like. Has been widely applied to various medical fields in recent years, and has wide application space and development prospect. The method for preparing PRP mainly comprises a primary centrifugation method, a secondary centrifugation method, a tertiary centrifugation method and the like, and the most widely used method mainly comprises the secondary centrifugation method. The existing suit and equipment for preparing PRP need to carry out operations such as transferring blood cells for many times and extracting PRP as a product in the preparation process, the preparation process is complicated, the operations are carried out manually, the problems of product pollution and overlong preparation time exist, and secondly, as most containers used in centrifugation are of a tubular or cup-shaped structure, the positions of boundary layers are difficult to judge and control due to the fact that the thicknesses of the boundary layers of blood plasma and red blood cells are thinner after blood centrifugation and layering, and finally the prepared PRP is unstable in enrichment multiple, less in PRP collection amount, higher in red blood cell residue and the like. Disclosure of utility model The utility model aims to solve the technical problems of overcoming the defects of the prior art, providing a microfluidic chip, solving the problem that the position of a boundary layer is difficult to judge and control due to the fact that the thickness of the boundary layer between blood plasma and red blood cells is thinner after blood centrifugation and delamination, and finally preparing platelet-rich blood plasma with stable PRP enrichment multiple, more PRP collection quantity and less red blood cell residue. The utility model solves the problems that the technical proposal adopted is as follows: The utility model provides a micro-fluidic chip, includes upper cover and separating vessel, and the upper cover is installed on separating vessel, and separating vessel top is equipped with the seal, and upper cover bottom and seal butt are enclosed to establish with the seal and are formed the inner chamber, and separation chipset is installed to the intracavity, separation chipset top and upper cover top butt, bottom and seal butt, and separation chipset includes multilayer separation chip, and separation chip is used for the separation of blood cell in the blood and enrichment PRP plasma. Preferably, the separation chip comprises a blood inlet, a blood channel arranged in a mirror image mode, a plurality of outlets and a blood cell outlet arranged in a mirror image mode, one end of the blood channel is communicated with the blood inlet, and the other end of the blood channel is communicated with the blood cell outlet. Preferably, each blood channel is provided with a contraction section, a throat and an expansion section which are communicated in sequence, one end of the expansion section is communicated with the throat, and the other end of the expansion section is communicated with a blood cell outlet; the expansion section is provided with micro-channels in mirror image near the throat, one end of the micro-channel is communicated with the expansion section, and the other end of the micro-channel is communicated with the PRP outlet. Preferably, the seal layer is provided with a plurality of PRP inlets and blood cell inlets arranged in a mirror image mode, the PRP inlets are aligned with the PRP outlets, and the blood cell inlets are aligned with the blood cell outlets. Preferably, the separation vessel comprises a PRP collection zone, a blood cell collection zone, a PRP inlet in communication with the PRP collection zone, and a blood cell inlet in communication with the blood cell collection zone; the side wall of the separation vessel at the PRP collecting zone is provided with an extraction port. Preferably, the top of the upper cover is provided with an upper cover injection opening, and the upper cover injection opening is aligned with the blood inlet. Compared with the prior art, the