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CN-224212640-U - Double-cover square culture dish

CN224212640UCN 224212640 UCN224212640 UCN 224212640UCN-224212640-U

Abstract

The utility model discloses a double-cover square culture dish which comprises a dish body and two covers matched with the dish body, wherein the dish body is of a box body structure with an opening at the top, the cover body A and the cover body B are of box body structures with openings at the bottom and are used for covering the openings of the dish body, a plurality of partition strips protruding into the dish body are further arranged on the inner surface of the cover body B, and gaps are formed between the partition strips and the inner bottom surface of the dish body. The utility model not only solves the problem that the droplet paths are crossed in the tilting process of the dish body when the satisfaction detection experiment of the bactericidal antibody is carried out by using the common culture dish, but also improves the accuracy of the experimental result, and has the characteristics of simple structure, low cost, convenience and practicability.

Inventors

  • ZHANG CHAO
  • XIE RONGXIAN
  • Li Ruoyue
  • HUANG FANG

Assignees

  • 复星安特金(成都)生物制药股份有限公司

Dates

Publication Date
20260508
Application Date
20250418

Claims (12)

  1. 1. The double-cover square culture dish is characterized by comprising a dish body (1) and two kinds of cover bodies matched with the dish body (1), wherein the cover body A (2) and the cover body B (3) are arranged, the dish body (1) is of a box body structure with an opening at the top, the cover body A (2) and the cover body B (3) are of box body structures with openings at the bottom so as to be used for covering the openings of the dish body (1), a plurality of partition strips (4) protruding into the dish body (1) are further arranged on the inner surface of the cover body B (3), and gaps (5) are formed between the partition strips (4) and the inner bottom surface of the dish body (1).
  2. 2. Double-lid square culture dish according to claim 1, characterized in that the parting bead (4) is in a strip-like structure.
  3. 3. The double-lid square culture dish according to claim 2, wherein the strip-like structure is a rectangular parallelepiped structure.
  4. 4. A double-lid square culture dish according to claim 3, wherein the width of the division bar (4) is 5-25 mm, and the length of the division bar (4) is 10-30 mm shorter than the side length of the dish body (1).
  5. 5. Double-lid square culture dish according to claim 4, characterized in that the width of the division bar (4) is 10-20 mm.
  6. 6. A double-lid square culture dish according to claim 3, characterized in that the height of the division bar (4) is 3/5~4/5 of the depth of the dish body (1).
  7. 7. The double-cover square culture dish according to claim 6, wherein the height of the division bars (4) is 2/3-4/5 of the depth of the dish body (1).
  8. 8. Double-lid square petri dish according to claim 1, characterized in that the spacers (4) are arranged at intervals and parallel to the side walls of the lid B (3).
  9. 9. The double-cover square culture dish according to claim 8, wherein the division bars (4) are uniformly distributed in the cover body B (3), and the interval distance between two adjacent division bars (4) is 5-10 mm.
  10. 10. Double-lid square culture dish according to claim 8, characterized in that the number of the division bars (4) is 3-10.
  11. 11. Double-lid square culture dish according to claim 1, characterized in that the dish body (1) and both covers are made of plastic or glass.
  12. 12. Double-lid square culture dish according to any one of claims 1 to 11, characterized in that the parting bead (4) is integrally formed with the lid body B (3).

Description

Double-cover square culture dish Technical Field The utility model provides a double-cover square culture dish, in particular relates to experimental equipment for culturing microorganisms by a solid culture medium, and belongs to the technical field of experimental equipment. Background In the development of bacterial vaccines, it is often necessary to conduct an evaluation of the immunogenicity of the serum after immunization of animals or humans with bacterial vaccines, where a critical experiment is to detect the level of vaccine-induced bactericidal antibody titres against the targeted bacteria in the serum. In bactericidal antibody titer testing experiments, it is generally necessary to mix bacteria, complement and gradient diluted serum, then drop the incubated mixture onto a culture plate containing a specific antibiotic, and tilt the plate to cause the liquid to flow in strips. After a period of incubation under suitable conditions, viable bacteria are allowed to form visible colonies. The number of surviving colonies was counted using an automatic counter or manually, and the bactericidal titer was defined as the highest dilution of serum that killed 50% of the bacteria, based on the number of complement controls Kong Junla without serum. The experimental result shows that the serum dilution gradient is increased from left to right, and the serum sterilization gradient is weakened, so that bacterial colonies gradually appear, and the serum sterilization titer can be judged. In the practical operation process of the bactericidal antibody titer detection experiment, a common round culture dish is used, only the middle wider part can be utilized in the experiment, and only a limited number of mixed liquid drops can be dripped for comparison experiment, so that the detection flux is lower. In the tilting process of the common square culture dish after dripping mixed liquid drops, the liquid drops are difficult to ensure to stably and uniformly flow forward to form a colony belt with uniform distribution, the liquid drops at different positions are in a non-blocking structure, and are easy to cross together after tilting, so that the colony belt is not clear after culturing, and the judgment of an experimental result is seriously influenced. In the prior art, a partition mode is mostly adopted to divide the culture dish into a plurality of culture holes by arranging partition in the space in the culture dish, for example, a partition type biological culture dish disclosed in the utility model patent with the publication number of CN209619350U only divides the culture dish main body into two parts, which cannot meet the experimental needs, and a disposable detachable partition type square culture dish disclosed in the utility model patent with the publication number of CN220550172U is arranged in a porous mode with a cross lattice, so that a plurality of clear colony bands cannot be formed. It can be seen that the prior art does not provide a solution to the above or related problems. Disclosure of utility model The utility model aims to utilize the cover body B which is matched with the dish body and is internally provided with a plurality of parting strips, so that a solid culture medium which is heated and melted in the dish body forms spaced grooves which can be used for culturing a bacterial colony belt after solidification, thereby solving the problem that a common culture dish cannot form a clear and non-crossed bacterial colony belt in a bactericidal antibody titer detection experiment, and further ensuring the reliability of an experiment result. The utility model is realized by the following technical scheme: The double-cover square culture dish comprises a dish body and two covers matched with the dish body, wherein the dish body is of a box body structure with an opening at the top, the cover body A and the cover body B are of box body structures with openings at the bottom and are used for covering the openings of the dish body, a plurality of partition strips protruding into the dish body are further arranged on the inner surface of the cover body B, and gaps are formed between the partition strips and the inner bottom surface of the dish body. Preferably, the parting bead is in a strip structure, wherein a cuboid structure is preferred, the inside of the strip structure can be solid or hollow, and the four corners of the strip structure protruding into the dish body can be right-angle structures or round-angle structures. Preferably, the width of the parting bead is 5-25 mm (10-20 mm is further preferred), and the length of the parting bead is 10-30 mm shorter than the side length of the dish body. Preferably, the height of the parting bead is 3/5~4/5 (preferably 2/3-4/5) of the depth of the dish body (namely the vertical distance between the highest point of the side wall of the dish body and the inner bottom surface), and the height of the gap between the parting bead and the inner bott